苦参碱对宫颈癌细胞株和宫颈上皮组织中Pin1和Cyclin D1的表达影响

目的:研究苦参碱对宫颈癌细胞株和宫颈上皮组织中Pin1和Cyclin D1的表达影响。方法:采用He La宫颈癌细胞株分为实验组和对照组,实验组采用苦参碱处理48 h,对照组采用等量正常细胞悬液处理,正常宫颈上皮组织作为正常对照组,利用免疫组化法检测Pin1、Cyclin D1蛋白表达情况,并进行比较,同时计算Pin1、Cyclin D1蛋白表达的相关性。结果 :(1)Pin1、Cyclin D1在正常宫颈上皮组织中表达呈阴性,在宫颈癌细胞株表达呈阳性;实验组Pin1为低表达,Cyclin D1表达为弱阳性;对照组Pin1为高表达,Cyclin D1表达为强阳性;(2)Pin1在正常宫颈上皮组织中无表达,实验组Pin1表达率为67.5%,与对照组(92.5%)比较,明显降低,差异有统计学意义(P<0.05);(3)Cyclin D1在正常宫颈上皮组织中无表达,实验组Cyclin D1表达率为65%,与对照组(85%)比较,明显降低,差异有统计学意义(P<0.05);(4)宫颈癌细胞株中Pin1和Cyclin D1表达均为阳性的有33例(82.5%),均为阴性的有12例(30%)。Pin1阳性但Cyclin D1阴性的有3例(7.5%),Pin1阴性但Cyclin D1阳性的有5例(12.5%),Pin1和Cyclin D1表达呈正相关(r=0.715,P<0.01)。结论:苦参碱能够明显降低宫颈癌细胞株中Pin1和Cyclin D1的表达,抑制肿瘤细胞增殖,对临床具有指导意义,值得临床推广。

Pin1 overexpression in colorectal cancer and its correlation with aberrant β-catenin expression

AIM： To investigate clinical significance of Pin1 and β-catenin expression in colorectal cancers and to demonstrate the relationship of their expression. METHODS： The role of Pin1 and β-catenin protein in colorectal tumorigenesis and their clinicopathologic significance were analyzed by immunohistochemistry, and correlation between Pin1 and β-catenin protein expressions was also studied in 124 patients with colorectal cancer who were surgically treated. RESULTS： Normal colonic epithelium either failed to express or showed focal and weak expression of Pin1 and β-catenin. Overexpression of Pin1 and β-catenin protein was found in 23 （18.54%） and 50 （40.3%） of 124 colorectal cancers, respectively. Overexpression of both proteins was not related to the lymph node metastasis, tumor stage and survival period after excision. Survival analysis results indicated that tumor stage was a valuable predictor of survival. Interestingly, a significant correlation was found between Pin1 and β-catenin protein expression. CONCLUSION： Overexpression of Pin1 and β-catenin may be closely related with the development and/or progression of colorectal carcinoma and further supports that Pin1 overexpression might contribute to the upregulation of β-catenin.