目的:调查广西黑衣壮族人群4个短串联重复序列(short tandem repeats,STR)基因座:D5S818、D7S820、D13S317、vWA的群体遗传分布资料,探讨7个民族间的遗传关系。方法:应用荧光标记STR基因扫描技术分析152名广西黑衣壮族无关个体。以...目的:调查广西黑衣壮族人群4个短串联重复序列(short tandem repeats,STR)基因座:D5S818、D7S820、D13S317、vWA的群体遗传分布资料,探讨7个民族间的遗传关系。方法:应用荧光标记STR基因扫描技术分析152名广西黑衣壮族无关个体。以遗传距离和系统发生树来分析7个民族间遗传关系。结果:4个STR基因座平均观察杂合度为0.7814;平均多态信息总量为0.7375;累积个体识别力达0.9928809,累积非父排除率达0.9928809。新疆乌孜别克族、新疆维吾尔族、甘肃裕固族、云南白族、广西黑衣壮族、浙江畲族聚为一群,东部蒙古族自成一群。结论:广西黑衣壮族的4个STR基因座属高度遗传多态性标记;新疆乌孜别克族和维吾尔族、广西黑衣壮族和浙江畲族遗传关系较近。展开更多
Simple sequences repeat (SSR) molecular maker, as a new type of DNA molecular marker, the second generation based on the polymerase chain reaction (PCR), is valuable and of great potential as genetic markers for i...Simple sequences repeat (SSR) molecular maker, as a new type of DNA molecular marker, the second generation based on the polymerase chain reaction (PCR), is valuable and of great potential as genetic markers for its characteristics of abundant quantity, high polymorphic, reproducibility, specific site amplification, high occurring frequency, and co-dominant inheritance etc. This paper outlined its principles and characteristics, and introduced its application to variety identification, phylogenetic relationship analysis, genetic diversity analysis, DNA fingerprinting and linkage map constructing etc. in recent years in Citrus and its close relatives.展开更多
文摘目的:调查广西黑衣壮族人群4个短串联重复序列(short tandem repeats,STR)基因座:D5S818、D7S820、D13S317、vWA的群体遗传分布资料,探讨7个民族间的遗传关系。方法:应用荧光标记STR基因扫描技术分析152名广西黑衣壮族无关个体。以遗传距离和系统发生树来分析7个民族间遗传关系。结果:4个STR基因座平均观察杂合度为0.7814;平均多态信息总量为0.7375;累积个体识别力达0.9928809,累积非父排除率达0.9928809。新疆乌孜别克族、新疆维吾尔族、甘肃裕固族、云南白族、广西黑衣壮族、浙江畲族聚为一群,东部蒙古族自成一群。结论:广西黑衣壮族的4个STR基因座属高度遗传多态性标记;新疆乌孜别克族和维吾尔族、广西黑衣壮族和浙江畲族遗传关系较近。
文摘Simple sequences repeat (SSR) molecular maker, as a new type of DNA molecular marker, the second generation based on the polymerase chain reaction (PCR), is valuable and of great potential as genetic markers for its characteristics of abundant quantity, high polymorphic, reproducibility, specific site amplification, high occurring frequency, and co-dominant inheritance etc. This paper outlined its principles and characteristics, and introduced its application to variety identification, phylogenetic relationship analysis, genetic diversity analysis, DNA fingerprinting and linkage map constructing etc. in recent years in Citrus and its close relatives.