The Food and Agriculture Organization has highlighted pineapple as one of the most important tropical fruits. Since classical pineapple breeding is difficult, biotechnology has emerged as an attractive instrument. We ...The Food and Agriculture Organization has highlighted pineapple as one of the most important tropical fruits. Since classical pineapple breeding is difficult, biotechnology has emerged as an attractive instrument. We obtained two new pineapple somaclonal variants derived from in vitro culture of cv. Red Spanish Pinar: P3R5 and Dwarf. The AFLP analysis revealed an existing genetic distance. So far 44 phenotype indicators selected due to their relation to a wide range of important agricultural, morphological and physiological processes have been evaluated. P3R5 differed from the donor in 19 variables (19/44;43.18%), while Dwarf varied in 31 indicators (31/44;70.45%). The number of shoots was significantly different among the three plant materials. Dwarf showed two shoots per plant while P3R5 and the donor did not form any shoots. We also observed that water use efficiency, chlorophyll b concentration, total chlorophyll concentration, transpiration rate, chlorophyll a concentration, thickness of leaf photosynthetic parenchyma, fruit mass with crown, content of free phenolics and superoxide dismutase specific activity were also very different among the three plant materials. The Euclidean distances of each somaclonal variant to the donor plant material taking into consideration the genotype (AFLP) and the phenotype evaluations were also calculated. Regarding the genotype information, P3R5 is separated from cv. Red Spanish Pinar by 2.83 units of Euclidean distance, and Dwarf by 3.00 units. However, the phenotype indicators revealed higher differences: 3.74 in P3R5 and 4.71 in Dwarf. To our knowledge, this is the first report of a comprehensive analysis of pineapple somaclonal variants.展开更多
为筛选适合澳洲坚果(Macadamia ternifolia F. Muell.)幼龄果园间套种的菠萝[Ananas comosus(Linn.)Merr.]品种,利用随机区组设计,对台农16号、台农17号、金菠萝、台农4号4个优良菠萝品种进行试种试验,调查植株农艺性状、果实性状及品质...为筛选适合澳洲坚果(Macadamia ternifolia F. Muell.)幼龄果园间套种的菠萝[Ananas comosus(Linn.)Merr.]品种,利用随机区组设计,对台农16号、台农17号、金菠萝、台农4号4个优良菠萝品种进行试种试验,调查植株农艺性状、果实性状及品质,并进行相关性分析。结果表明,在澳洲坚果幼龄果园下套种,台农16号菠萝植株高度、叶片宽度、平均单果重、可溶性固形物含量、可溶性糖含量均最高,果肉纤维少,质地嫩滑,风味清甜。综合评价,台农16号是作为澳洲坚果间套种的最适菠萝品种。展开更多
Fruit is one of the essential sources of human nutrition.Consumers around the world need to be able to purchase fruit of reliable flavor and nutritional quality.Physical appearance and physicochemical properties play ...Fruit is one of the essential sources of human nutrition.Consumers around the world need to be able to purchase fruit of reliable flavor and nutritional quality.Physical appearance and physicochemical properties play a key role in determining desirable quality and flavor.However,for some fruits such as watermelon,durian,pineapple,it is very hard to determine quality and flavor by external appearance.Therefore,a practical method to predict physical and physicochemical properties of fruit needs to be developed.In this study,a computerized technique is investigated to determine pineapple grades and their physical and physicochemical properties,including ripeness,total soluble solids,pH value and water content.The results reveal that by grading using pulp characteristics it is possible to classify pineapples into three distinct groups,which are significantly different in TSS,pH value and water content.In addition,predicting pineapple grades using flicking sounds and signal processing demonstrates that pineapples classified as grade 1 and grade 3 are significantly different in TSS,pH value and water content.This suggests that the estimation of the texture of pineapple pulp and its physicochemical properties can be performed prior to cutting.Therefore,it is feasible to develop an automated grading technique that can be used to determine pineapple quality as accurately as destructive grading to predict pineapple grades,texture and physicochemical properties.展开更多
文摘The Food and Agriculture Organization has highlighted pineapple as one of the most important tropical fruits. Since classical pineapple breeding is difficult, biotechnology has emerged as an attractive instrument. We obtained two new pineapple somaclonal variants derived from in vitro culture of cv. Red Spanish Pinar: P3R5 and Dwarf. The AFLP analysis revealed an existing genetic distance. So far 44 phenotype indicators selected due to their relation to a wide range of important agricultural, morphological and physiological processes have been evaluated. P3R5 differed from the donor in 19 variables (19/44;43.18%), while Dwarf varied in 31 indicators (31/44;70.45%). The number of shoots was significantly different among the three plant materials. Dwarf showed two shoots per plant while P3R5 and the donor did not form any shoots. We also observed that water use efficiency, chlorophyll b concentration, total chlorophyll concentration, transpiration rate, chlorophyll a concentration, thickness of leaf photosynthetic parenchyma, fruit mass with crown, content of free phenolics and superoxide dismutase specific activity were also very different among the three plant materials. The Euclidean distances of each somaclonal variant to the donor plant material taking into consideration the genotype (AFLP) and the phenotype evaluations were also calculated. Regarding the genotype information, P3R5 is separated from cv. Red Spanish Pinar by 2.83 units of Euclidean distance, and Dwarf by 3.00 units. However, the phenotype indicators revealed higher differences: 3.74 in P3R5 and 4.71 in Dwarf. To our knowledge, this is the first report of a comprehensive analysis of pineapple somaclonal variants.
文摘为筛选适合澳洲坚果(Macadamia ternifolia F. Muell.)幼龄果园间套种的菠萝[Ananas comosus(Linn.)Merr.]品种,利用随机区组设计,对台农16号、台农17号、金菠萝、台农4号4个优良菠萝品种进行试种试验,调查植株农艺性状、果实性状及品质,并进行相关性分析。结果表明,在澳洲坚果幼龄果园下套种,台农16号菠萝植株高度、叶片宽度、平均单果重、可溶性固形物含量、可溶性糖含量均最高,果肉纤维少,质地嫩滑,风味清甜。综合评价,台农16号是作为澳洲坚果间套种的最适菠萝品种。
文摘Fruit is one of the essential sources of human nutrition.Consumers around the world need to be able to purchase fruit of reliable flavor and nutritional quality.Physical appearance and physicochemical properties play a key role in determining desirable quality and flavor.However,for some fruits such as watermelon,durian,pineapple,it is very hard to determine quality and flavor by external appearance.Therefore,a practical method to predict physical and physicochemical properties of fruit needs to be developed.In this study,a computerized technique is investigated to determine pineapple grades and their physical and physicochemical properties,including ripeness,total soluble solids,pH value and water content.The results reveal that by grading using pulp characteristics it is possible to classify pineapples into three distinct groups,which are significantly different in TSS,pH value and water content.In addition,predicting pineapple grades using flicking sounds and signal processing demonstrates that pineapples classified as grade 1 and grade 3 are significantly different in TSS,pH value and water content.This suggests that the estimation of the texture of pineapple pulp and its physicochemical properties can be performed prior to cutting.Therefore,it is feasible to develop an automated grading technique that can be used to determine pineapple quality as accurately as destructive grading to predict pineapple grades,texture and physicochemical properties.