Establishment of Real-time Fluorescent Quantitative PCR for Pineapple mealybug wilt associated virus-3

[ Objective ] The paper aimed to establish a real-time fluorescent quantitative PCR （qPCR） detection method for Pineapple mealybug wilt associated vi- rus-3 （ PMWaV3 ）. [ Method] Specific TaqMan probe and primers were designed and synthesized according to the conserved sequence of coat protein（CP） gene of PMWaV-3, and the standard curve was established after optimizing the amplification condition of qPCR. [ Result] The results showed that the method was specific for the detection of PMWaV-3, and the sensitivity of the present method was about 10 times higher compared to general RT-PCR ; the variation coefficients of intra- assay and inter-assay were less than 1.73, respectively. [ Conclusion] The qPCR is an easy, fast and reliable method for quantitative detection of PMWaV-3.

菠萝凋萎相关病毒-3实时荧光定量PCR检测方法的建立

【目的】建立菠萝凋萎相关病毒-3实时荧光定量PCR检测方法。【方法】根据PMWa V-3外壳蛋白基因的保守区域设计特异性引物和探针,通过优化PMWa V-3实时荧光定量PCR检测体系,构建以重组质粒为标准品的标准曲线,建立起PMWa V-3实时荧光定量PCR检测方法。【结果】该方法能特异性地检测PMWa V-3,且灵敏度是普通RTPCR的10倍;该方法重复性良好,组间和组内变异系数均小于1.73。【结论】建立的实时荧光定量PCR方法是一种快速、简便、可信度高的PMWa V-3定量检测方法。

菠萝凋萎病毒研究进展

菠萝凋萎病毒的研究对于从根本上控制该病害具有重要意义。文章介绍了菠萝凋萎病的症状和危害,综述了菠萝凋萎病毒的分类、检测、基因组结构、致病机制和防治策略等方面的研究现状和动向,对存在问题进行了探讨。