[Objectives] The aim was to study chemical components of cultivated and wild Pinellia ternate(Thunb.) Breit and Typhonium flagelliforme(Lodd.) Blume by metabolomics technology based on UPLC-ESI-HRMS^n. [Methods] Chemi...[Objectives] The aim was to study chemical components of cultivated and wild Pinellia ternate(Thunb.) Breit and Typhonium flagelliforme(Lodd.) Blume by metabolomics technology based on UPLC-ESI-HRMS^n. [Methods] Chemical components of 27 samples of cultivated and wild P. ternate and T. flagelliforme were detected by using UPLC-ESI-HRMS^ntechnology. Differential markers between P. ternate and T. flagelliforme as well as cultivated and wild P. ternate were screened and identified by principal component analysis(PCA) and orthogonal partial least square-discriminate analysis(OPLS-DA). [Results]12 components of P. ternate(cultivated and wild variety) and T.flagelliforme and three components of cultivated and wild P. ternate were screened and identified by OPLS-DA. N-Acetyl-6-hydroxy-L-tryptophan and N-Phenylacetylglutamic were not detected in P. ternate,and these two components can be used as potential markers of P. ternate and T. flagelliforme. [Conclusions]UPLC-ESI-HRMS^n-based metabolomics technology could be used to quickly identify the chemical constituents of P. ternate and effectively distinguish P. ternate and T. flagelliforme,which has reference significance for the quality control of P. ternate.展开更多
Based on literature reviews and analysis of research reports on Pinellia ternata found locally and abroad in recent years,this article summarizes and arranges them.The research on Pinellia ternata mainly focuses on it...Based on literature reviews and analysis of research reports on Pinellia ternata found locally and abroad in recent years,this article summarizes and arranges them.The research on Pinellia ternata mainly focuses on its cultivation,tissue culture,and so on.There are only a few research on its active components and its regulation mechanism.The wild resources of Pinellia ternata are gradually decreasing,hence it is urgent to take effective measures to protect these wild resources as well as to establish germplasm resources bank and nursery.In order to meet the needs of the domestic market,it is necessary to investigate the distribution of wild Pinellia ternata resources,explore the best growing environment and conditions,artificially cultivate Pinellia ternata,as well as implement resource industrialization,sustainable development,and utilization.展开更多
基金Supported by Preferred Funding Plan of Science and Technology Activities Project for Overseas Students in Sichuan Province(2018-68)Key Project for Central Universities of Southwest Minzu University in 2018(2018NZD18)+1 种基金Research Project of Sichuan Province(2019YFS0174,2019YFS0191,2018JY0069)Innovative Scientific Research Project for Postgraduates of Southwest Minzu University(CX2017SZ092)
文摘[Objectives] The aim was to study chemical components of cultivated and wild Pinellia ternate(Thunb.) Breit and Typhonium flagelliforme(Lodd.) Blume by metabolomics technology based on UPLC-ESI-HRMS^n. [Methods] Chemical components of 27 samples of cultivated and wild P. ternate and T. flagelliforme were detected by using UPLC-ESI-HRMS^ntechnology. Differential markers between P. ternate and T. flagelliforme as well as cultivated and wild P. ternate were screened and identified by principal component analysis(PCA) and orthogonal partial least square-discriminate analysis(OPLS-DA). [Results]12 components of P. ternate(cultivated and wild variety) and T.flagelliforme and three components of cultivated and wild P. ternate were screened and identified by OPLS-DA. N-Acetyl-6-hydroxy-L-tryptophan and N-Phenylacetylglutamic were not detected in P. ternate,and these two components can be used as potential markers of P. ternate and T. flagelliforme. [Conclusions]UPLC-ESI-HRMS^n-based metabolomics technology could be used to quickly identify the chemical constituents of P. ternate and effectively distinguish P. ternate and T. flagelliforme,which has reference significance for the quality control of P. ternate.
基金Supported by Ankang Science and Technology Bureau,Shaanxi Province(2018ak03-ll)National Traditional Chinese Medicine Characteristic Technology Inheritor(t20194828003).
文摘Based on literature reviews and analysis of research reports on Pinellia ternata found locally and abroad in recent years,this article summarizes and arranges them.The research on Pinellia ternata mainly focuses on its cultivation,tissue culture,and so on.There are only a few research on its active components and its regulation mechanism.The wild resources of Pinellia ternata are gradually decreasing,hence it is urgent to take effective measures to protect these wild resources as well as to establish germplasm resources bank and nursery.In order to meet the needs of the domestic market,it is necessary to investigate the distribution of wild Pinellia ternata resources,explore the best growing environment and conditions,artificially cultivate Pinellia ternata,as well as implement resource industrialization,sustainable development,and utilization.
