The inheritance and expression of bar gene and pinII gene were studied in three transgenic rice lines and their F2 hybrid populations, which were created through hybridization with a PGMS line, ZAU11S. By Basta painti...The inheritance and expression of bar gene and pinII gene were studied in three transgenic rice lines and their F2 hybrid populations, which were created through hybridization with a PGMS line, ZAU11S. By Basta painting, PCR analysis and determining of the inhibitory trypsin activity, the results show that bar gene and pinII gene in rice F2 population fit the simple Mendel's low of inheritance and close linkage, but a few plants in F2 have not sufficiently expressed. The wound inducible pin II gene has an expression regulated spatially and temporally, and the signal transduction pathway is not only upward, but also downward. The inducible expression of pin II in different rice transgenic lines is not completely coincident.展开更多
基金sponsored by the National 863 Program of China(101-01-01-01-3)the National Natural Science Foundation of China(39870421)the Key Project in Zhejiang Province(2003C22007).
文摘The inheritance and expression of bar gene and pinII gene were studied in three transgenic rice lines and their F2 hybrid populations, which were created through hybridization with a PGMS line, ZAU11S. By Basta painting, PCR analysis and determining of the inhibitory trypsin activity, the results show that bar gene and pinII gene in rice F2 population fit the simple Mendel's low of inheritance and close linkage, but a few plants in F2 have not sufficiently expressed. The wound inducible pin II gene has an expression regulated spatially and temporally, and the signal transduction pathway is not only upward, but also downward. The inducible expression of pin II in different rice transgenic lines is not completely coincident.
