Extraction and Content Determination of Shikimic Acid in Pinus elliottii Engelm

[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC.The HPLC conditions were as follows;Alltima NH2 （5 μm,4.6 mm × 150 mm） column separation;the mobile phase was acetonitrile-2%H3PO4 （90：10）;the flowing velocity was 1 ml/min;test wavelength was 213 nm,the width of belt was 16 nm;reference wavelength was 300 nm,the width of belt was 80 nm.[Result] By the single factor and orthogonal tests,the optimum conditions were found as follows：ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1：25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%.[Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.

Growth Responses of Young Slash Pine (Pinus elliottii Engelm.) to N, P and K Fertilizers in Red-Yellow Soil in Jiangxi Province, China

During 1991~1995, the growth responses of young slash pine to N, P and K fertilizers and rational rate and ratio of fertilizers in yellow-red soil at Fengshushan Forest Farm, Jiangxi Province were studied. N fertilizer decreased the growth of slash pine, while P not only significantly improved the increment of bottom diameter, height and crown diameter, but also caused earlier canopy closure of the stand. It also had a sustainable effect in the next two years. Slash pine had the most significant response to the treatment of 200 kg P2O5 ha-1, in which tree height, bottom diameter and crown diameter (EW and SN) were increased by 22%, 35%, 20% and 18% in 1994, 21%, 25%, 10% and 10% in 1995, respectively, compared to the control.Although there was no remarkable response to K fertilizer, good growth response to the application of 100 kg P2O5 plus 100 kg K2O ha-1 existed and tree height, bottom diameter and crown diameter (EW and SN)were increased by 31%, 14%, 23% and 21%, respectively, in 1993, compared to the control.

Effects of Ground Mulching Treatments on Soil Moisture Content in Second-generation Seed Garden of Pinus elliottii

Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that： （1） there were extreme differences in soil moisture content Ⅰ and soil moisture content Ⅲ, but no obvious difference in soil moisture content II was observed; and （2） the high-to-low order of soil moisture content Ⅰ under different ground mulching treatments was white film〉sawdust〉black film〉the control, and the highest value was higher than the lowest one by 26.69%; the high-to-low order of soil moisture content Ⅱ under different ground mulching treatments was white film〉black film〉sawdust〉the control, and the highest value was higher than the lowest one by 20.64%; and the high-to-low order of soil moisture content Ⅲ under different ground mulching treatments was black film〉white film〉sawdust〉the control, and the highest value was higher than the lowest one by 26.61%.