AIM: To study the effects of Pinus massoniana bark extract (PMBE) on cell proliferation and apoptosis of human hepatoma BEL-7402 cells and to elucidate its molecular mechanism.METHODS: BEL-7402 cells were incubated wi...AIM: To study the effects of Pinus massoniana bark extract (PMBE) on cell proliferation and apoptosis of human hepatoma BEL-7402 cells and to elucidate its molecular mechanism.METHODS: BEL-7402 cells were incubated with various concentrations (20-200 μg/mL) of PMBE for different periods of time. After 48 h, cell proliferation was determined by 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptosis was evaluated by morphological observation, agarose gel electrophoresis,and flow cytometry analysis. Possible molecular mechanisms were primarily explored through immunohistochemical staining.RESULTS: PMBE (20-200 μg/mL) significantly suppressed BEL-7402 cell proliferation in a time- and dose-dependent manner. After treatment of BEL-7402 cells with 160 μg/mL PMBE for 24, 48, or 72 h, a typical apoptotic 'DNA ladder'was observed using agarose gel electrophoresis. Nuclear condensation and boundary aggregation or split, apoptotic bodies were seen by fluorescence and electron microscopy.Sub-G1 curves were displayed by flow cytometry analysis.PMBE decreased the expression levels of Bcl-2 protein in a time-dependent manner after treatment of cells with 160 μg/mL PMBE.CONCLUSION: PMBE suppresses proliferation of BEL-7402 cells in a time- and dose-dependent manner and induces cell apoptosis by possibly downregulating the expression of the bcl-2 gene.展开更多
目的:评价马尾松树皮提取物(PMBE)和葡萄籽提取物(GSE)对根面牙本质耐酸蚀脱矿作用的影响。方法:表面一半被覆盖的根面牙本质块40个,被随机分为4组(n=10),分别用去离子蒸馏水(DDW)溶液、0.1%NaF溶液、12%PMBE溶液和12%GSE溶液作为实验溶...目的:评价马尾松树皮提取物(PMBE)和葡萄籽提取物(GSE)对根面牙本质耐酸蚀脱矿作用的影响。方法:表面一半被覆盖的根面牙本质块40个,被随机分为4组(n=10),分别用去离子蒸馏水(DDW)溶液、0.1%NaF溶液、12%PMBE溶液和12%GSE溶液作为实验溶液,进行为期8 d的pH循环(实验溶液,酸性缓冲液和中性缓冲液)。显微CT测定各组未脱矿侧和脱矿侧牙本质的矿物密度(dentin mineral density,DMD),场发射扫描电镜观察各组pH循环后牙本质表面显微形貌。结果:牙本质的矿物密度显示DDW组脱矿侧和未脱矿侧牙本质矿物密度差(ΔDMD)198.64±59.97,NaF组为45.94±24.21,PMBE组为90.23±28.77,GSE组为105.07±29.53。PMBE组和GSE组ΔDMD均高于NaF组(P<0.05),低于DDW组(P<0.05),两者相互之间无显著差异(P>0.05)。扫描电镜见DDW组牙本质表面牙本质小管完全开放,NaF组牙本质小管基本封闭,PMBE组和GSE组牙本质小管口成梭形或狭长微裂隙开口。结论:PMBE和GSE均能提高牙本质的耐酸蚀脱矿作用。展开更多
文摘AIM: To study the effects of Pinus massoniana bark extract (PMBE) on cell proliferation and apoptosis of human hepatoma BEL-7402 cells and to elucidate its molecular mechanism.METHODS: BEL-7402 cells were incubated with various concentrations (20-200 μg/mL) of PMBE for different periods of time. After 48 h, cell proliferation was determined by 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptosis was evaluated by morphological observation, agarose gel electrophoresis,and flow cytometry analysis. Possible molecular mechanisms were primarily explored through immunohistochemical staining.RESULTS: PMBE (20-200 μg/mL) significantly suppressed BEL-7402 cell proliferation in a time- and dose-dependent manner. After treatment of BEL-7402 cells with 160 μg/mL PMBE for 24, 48, or 72 h, a typical apoptotic 'DNA ladder'was observed using agarose gel electrophoresis. Nuclear condensation and boundary aggregation or split, apoptotic bodies were seen by fluorescence and electron microscopy.Sub-G1 curves were displayed by flow cytometry analysis.PMBE decreased the expression levels of Bcl-2 protein in a time-dependent manner after treatment of cells with 160 μg/mL PMBE.CONCLUSION: PMBE suppresses proliferation of BEL-7402 cells in a time- and dose-dependent manner and induces cell apoptosis by possibly downregulating the expression of the bcl-2 gene.
文摘目的:评价马尾松树皮提取物(PMBE)和葡萄籽提取物(GSE)对根面牙本质耐酸蚀脱矿作用的影响。方法:表面一半被覆盖的根面牙本质块40个,被随机分为4组(n=10),分别用去离子蒸馏水(DDW)溶液、0.1%NaF溶液、12%PMBE溶液和12%GSE溶液作为实验溶液,进行为期8 d的pH循环(实验溶液,酸性缓冲液和中性缓冲液)。显微CT测定各组未脱矿侧和脱矿侧牙本质的矿物密度(dentin mineral density,DMD),场发射扫描电镜观察各组pH循环后牙本质表面显微形貌。结果:牙本质的矿物密度显示DDW组脱矿侧和未脱矿侧牙本质矿物密度差(ΔDMD)198.64±59.97,NaF组为45.94±24.21,PMBE组为90.23±28.77,GSE组为105.07±29.53。PMBE组和GSE组ΔDMD均高于NaF组(P<0.05),低于DDW组(P<0.05),两者相互之间无显著差异(P>0.05)。扫描电镜见DDW组牙本质表面牙本质小管完全开放,NaF组牙本质小管基本封闭,PMBE组和GSE组牙本质小管口成梭形或狭长微裂隙开口。结论:PMBE和GSE均能提高牙本质的耐酸蚀脱矿作用。
