研究了胡椒(Pipernigrum Linnaeus)和中粒种咖啡(Coffea canephora Pierre ex Froehner)种子在常温自然贮藏条件的萌发特性。结果表明,刚采收的胡椒种子和中粒种咖啡种子的含水量、发芽率、活力指数分别为29.32%和46.11%、88%和92.0%、1...研究了胡椒(Pipernigrum Linnaeus)和中粒种咖啡(Coffea canephora Pierre ex Froehner)种子在常温自然贮藏条件的萌发特性。结果表明,刚采收的胡椒种子和中粒种咖啡种子的含水量、发芽率、活力指数分别为29.32%和46.11%、88%和92.0%、1.46和5.57。自然贮藏20d,干燥脱水对胡椒种子的伤害较轻微,种子发芽率和活力指数仍高达82.7%和1.03;之后,由于严重脱水,种子发芽率和活力指数迅速下降;自然贮藏60d,种子含水量下降到10.91%时,完全丧失发芽力。自然贮藏30d,干燥脱水对中粒种咖啡种子的伤害较轻微,种子发芽率和活力指数仍高达80.5%和4.10;之后由于严重脱水,种子发芽率和活力指数急剧下降;自然贮藏90d,种子含水量下降到9.81%时,种子几乎完全丧失发芽力。由此认为,胡椒和中粒种咖啡是一种中间性种子,过度脱水使质膜结构和功能严重受损,最终导致种子活力的丧失。因此,在播种时,采用常温自然贮藏不超过20d的胡椒种子及贮藏不超过30d的中粒种咖啡种子为佳。展开更多
Background:Wound healing has being extensively investigated over the world.Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage,delaying in granulation tissue formation,re...Background:Wound healing has being extensively investigated over the world.Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage,delaying in granulation tissue formation,reducing in angiogenesis and decreasing in collagen reorganization.These facts consequently lead to chronic wound healing.Piper betle Linn(Betle)leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment.However,the effect of betle leaf on wound healing is not yet well elucidated.In this study,we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice.Methods:Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells.Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract.Malondialdehyde(MDA)was also evaluated to examine the products of lipid hydroperoxide(LPO)under conditions of with or without betle leaf extract treatment.Results:The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models.In addition,this extract significant decreased level of malondialdehyde(MDA)in liver of treated-mice compared with that in non-treated mice.Conclusions:Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds.展开更多
文摘研究了胡椒(Pipernigrum Linnaeus)和中粒种咖啡(Coffea canephora Pierre ex Froehner)种子在常温自然贮藏条件的萌发特性。结果表明,刚采收的胡椒种子和中粒种咖啡种子的含水量、发芽率、活力指数分别为29.32%和46.11%、88%和92.0%、1.46和5.57。自然贮藏20d,干燥脱水对胡椒种子的伤害较轻微,种子发芽率和活力指数仍高达82.7%和1.03;之后,由于严重脱水,种子发芽率和活力指数迅速下降;自然贮藏60d,种子含水量下降到10.91%时,完全丧失发芽力。自然贮藏30d,干燥脱水对中粒种咖啡种子的伤害较轻微,种子发芽率和活力指数仍高达80.5%和4.10;之后由于严重脱水,种子发芽率和活力指数急剧下降;自然贮藏90d,种子含水量下降到9.81%时,种子几乎完全丧失发芽力。由此认为,胡椒和中粒种咖啡是一种中间性种子,过度脱水使质膜结构和功能严重受损,最终导致种子活力的丧失。因此,在播种时,采用常温自然贮藏不超过20d的胡椒种子及贮藏不超过30d的中粒种咖啡种子为佳。
基金supported by Grants-in-Aid for Scientific Research under grant number KLEPT-14-02.
文摘Background:Wound healing has being extensively investigated over the world.Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage,delaying in granulation tissue formation,reducing in angiogenesis and decreasing in collagen reorganization.These facts consequently lead to chronic wound healing.Piper betle Linn(Betle)leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment.However,the effect of betle leaf on wound healing is not yet well elucidated.In this study,we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice.Methods:Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells.Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract.Malondialdehyde(MDA)was also evaluated to examine the products of lipid hydroperoxide(LPO)under conditions of with or without betle leaf extract treatment.Results:The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models.In addition,this extract significant decreased level of malondialdehyde(MDA)in liver of treated-mice compared with that in non-treated mice.Conclusions:Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds.
