The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study is...The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study isolated nine compounds from the EtOAc extract,all of which were obtained from Pithecellobium Mart.for the first time.Based on 1H-NMR and 13C-NMR data,the structures of these compounds,including two ladiosiplodins and two dihydroisocoumarins,were elucidated.展开更多
Aim To investigate the chemical constituents from the twigs and leaves of Pithecellobium clypearia Benth and their immunomodulatory effects. Methods The constituents were separated and purified by various chromatograp...Aim To investigate the chemical constituents from the twigs and leaves of Pithecellobium clypearia Benth and their immunomodulatory effects. Methods The constituents were separated and purified by various chromatographic methods and their structures were identified on the basis of spectral analysis. The immufiomodulatory effects of all the compounds were examined by a Con A-induced T lymphocytes proliferation assay. Results Eight compounds were isolated and identified as （-）- epigallocatechin （1）, （-）-5, 7, 3′, 4′, 5′-pentahydroxyflavan （2）, （-）-epigallocatechin-7-gallate （3）, （-）-5, 3′, 4′, 5′-tetrahydroxyfiavan- 7-gallate （4）, quercitin-3-O-α-L-rhamnpyranoside （5）, myricitin-3-O-α-L-rhamnpyranoside （6）, gallic acid （7）, and ethyl gallate （8）, respectively. Conclusion Compounds 3 and 8 were isolated from this genus for the first time, and compound 1 was isolated from this species for the first time. Compound 3 exhibited a strong inhibition on the T lymphocytes proliferation induced by Con A with an IC50 of 4.4 μmol·L^-1.展开更多
文摘The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study isolated nine compounds from the EtOAc extract,all of which were obtained from Pithecellobium Mart.for the first time.Based on 1H-NMR and 13C-NMR data,the structures of these compounds,including two ladiosiplodins and two dihydroisocoumarins,were elucidated.
文摘Aim To investigate the chemical constituents from the twigs and leaves of Pithecellobium clypearia Benth and their immunomodulatory effects. Methods The constituents were separated and purified by various chromatographic methods and their structures were identified on the basis of spectral analysis. The immufiomodulatory effects of all the compounds were examined by a Con A-induced T lymphocytes proliferation assay. Results Eight compounds were isolated and identified as （-）- epigallocatechin （1）, （-）-5, 7, 3′, 4′, 5′-pentahydroxyflavan （2）, （-）-epigallocatechin-7-gallate （3）, （-）-5, 3′, 4′, 5′-tetrahydroxyfiavan- 7-gallate （4）, quercitin-3-O-α-L-rhamnpyranoside （5）, myricitin-3-O-α-L-rhamnpyranoside （6）, gallic acid （7）, and ethyl gallate （8）, respectively. Conclusion Compounds 3 and 8 were isolated from this genus for the first time, and compound 1 was isolated from this species for the first time. Compound 3 exhibited a strong inhibition on the T lymphocytes proliferation induced by Con A with an IC50 of 4.4 μmol·L^-1.
