Daoist medicine,a special form of traditional Chinese medicine with distinct Daoist styles,has played an important role in the history of plague control in ancient China.Emerged from the pandemics at the end of the Ha...Daoist medicine,a special form of traditional Chinese medicine with distinct Daoist styles,has played an important role in the history of plague control in ancient China.Emerged from the pandemics at the end of the Han Dynasty(146 C.E.-220 C.E.),Daoism,the native Chinese religion,has always had a religious mission to end the spread of plagues;thus,it has actively participated in treating plagues through various techniques of medicine and religion.In the practice of plague treatment for thousands of years,Daoist medicine has developed a special etiology of plague that includes Qi-based theories(Qi is considered to be the force that informs and animates all things in Daoism,thus,plague is also considered to be caused by specific and hazardous Qi.)and worm-based theories,forming a strategy of plague control that includes prevention before an epidemic,active treatment during the epidemic,and recovery after the epidemic;these developed plague prevention methods combine isolation,disinfection,and drug use.Additionally,it is the first to practice a primitive immunoprophylaxis method that uses tissues and secretions of infected persons and animals to infect healthy individuals to gain disease immunity.It has formed a plague treatment method mainly based on drug treatment and ritual treatment,supplemented by acupuncture and physical treatment.It has left behind many effective drugs and prescriptions for plague treatment,some of which have a wide clinical application.Hence,Daoist medicine has made great contributions to plague control in Chinese history.Its strategies,methods,medicines,and prescriptions for plague control still have considerable theoretical and application today,especially in the coronavirus disease 2019(COVID-19)pandemic.展开更多
Inspired by recent environmental historical studies on animal extinctions and human-animal relations,this paper shifts scholarly attention from the plague-centered narrative of the great Pneumonic Plague Epidemics(191...Inspired by recent environmental historical studies on animal extinctions and human-animal relations,this paper shifts scholarly attention from the plague-centered narrative of the great Pneumonic Plague Epidemics(1910—11)to the fate of the plague host animals,Tarbagan marmots(Marmota sibirica>,and examines their ncar-extinction in Northwest Manchuria(Hulunbuir)from the 1900s to 1930s.Focusing on changing images of Tarbagan marmots from“inexpensive,”“sacred,”and“beneficial”in the pre-modem period to“valuable,”“dangerous,”and“noxious”in the early twentieth century,it argues that three interrelated factors:the international fur trade,pneumonic plagues,and environment changes together resulted in the“retreat of the marmots.”It also]uses this case study to help us better understand larger historical changes that occurred by contextualizing them in terms of human-marmot relations in Manchuria,China and beyond.展开更多
Plague is one of the severe infectious diseases which has had a huge impact on human society throughout history.Although there is abundant research on the disease,few studies focus on the concept of plague itself.It i...Plague is one of the severe infectious diseases which has had a huge impact on human society throughout history.Although there is abundant research on the disease,few studies focus on the concept of plague itself.It is generally believed that Shu Yi was first coined by Chinese doctors in the late 19th century,and it was closely related to the introduction of new medicine.This statement is not accurate,because plague was prevalent in Yunnan and Guangdong provinces in the late Qing Dynasty.At that time,people had already recognized the relationship between the outbreak of plague and the death of rats,and gradually named the epidemic disease as Shu Yi.This name was used by literati and doctors by coincidence.As the epidemic became more and more serious,more related works were widely disseminated,and Shu Yi changed from a folk name to a formal name.Later,with the help of the government’s active introduction of modern health and epidemic prevention mechanisms and the implementation of rodent control measures,Shu Yi became more popular and gradually recognized by all walks of society.The emergence and prevalence of Shu Yi created the history of zoonosis in China,and thus formed a new paradigm of naming human epidemics after related animal names.展开更多
The Great Plague:The Diary of Alice Paynton,London 1665-1666 adopts the realistic painting style and direct writing to the painful experience,describes the situation of the Great Plague in London truly from the perspe...The Great Plague:The Diary of Alice Paynton,London 1665-1666 adopts the realistic painting style and direct writing to the painful experience,describes the situation of the Great Plague in London truly from the perspective of children,and breaks people’s cognitive expectation of children’s literature,the construction of children’s Gray inner world is completed behind the true description of the Great Plague in London with the true brushwork,which presents a different world of childlike innocence.Despite the importance of epidemics writing in literature,the child’s diary perspective of them is one of the least developed of Plague Narrative.Only when authenticity and the child’s grey interior are integrated will we reveal a true picture of society as it was.展开更多
Chi Zijian’s novel White Snow Crow is about the pestis in Northeast China from the autumn and winter of 1910 to the spring of 1911,focusing on the living conditions of people in Fujiadian,Harbin,under the shadow of t...Chi Zijian’s novel White Snow Crow is about the pestis in Northeast China from the autumn and winter of 1910 to the spring of 1911,focusing on the living conditions of people in Fujiadian,Harbin,under the shadow of the pestis,and thus connects the vicissitudes of Harbin in the development of modern Chinese history.Re-reading this novel in the context of the post-epidemic era not only allows us to immerse ourselves in the specific temporal and spatial fields described in the text with an immersive reading mindset,feel the heavy impact that the disaster has brought to the people of Northeast China,but also provide us with a different perspective to observe the current social reality.In particular,the social problems shown by the novel through the pestis and the description of ordinary people’s life experience under the plague still deserve further discussion.展开更多
Season of the Witch is one of the great productions with all these characteristics which were introduced during August around China.The story was told during the final historical period of the Crusades.The Crusades we...Season of the Witch is one of the great productions with all these characteristics which were introduced during August around China.The story was told during the final historical period of the Crusades.The Crusades were a series of religious wars.The crusaders comprised military units from all over Western Europe.During 14th century Black Plague was threatened the Whole Europe.The Plague destroyed the Crusade and each town.People began to suspect some women which abused as witches should be take responsibilities.They put some women to death.The movie not only tell us a story,but lead us to find out lights from the dark world during the black age.展开更多
In view of the serious threat to people's health caused by the Coronavirus Disease 2019(COVID-19),according to the relevant spirit of the Notice on Establishing the Cooperation Mechanism of traditional Chinese and...In view of the serious threat to people's health caused by the Coronavirus Disease 2019(COVID-19),according to the relevant spirit of the Notice on Establishing the Cooperation Mechanism of traditional Chinese and Western Medicine in the Prevention and Treatment of COVID-19 and other Infectious Diseases issued by the National Health Commission of the People's Republic of China,National Administration of Traditional Chinese Medicine of the People's Republic of China,on the basis of our early clinical research of using press needle embedding(intradermal acupuncture)to prevent and treat related diseases,the symptoms,etiology and pathogenesis of the disease were analyzed.We try to use the simple and easy intradermal acupuncture therapy to prevent and assist the treatment of the disease and its complications,hoping to make a modest contribution to help patients relieve part of suffering,and also provide advice for the doctors in the treatment of the disease.展开更多
The glycoprotein H (gH) gene homologue of duck plague virus (DPV) was cloned by degenerate polymerase chain reaction (PCR) and sequenced. It was located immediately downstream from the thymidine kinase gene (TK...The glycoprotein H (gH) gene homologue of duck plague virus (DPV) was cloned by degenerate polymerase chain reaction (PCR) and sequenced. It was located immediately downstream from the thymidine kinase gene (TK). In addition, the 3'-end of the gene homologue to herpesvirus UL21 was located downstream from the gH gene. DPV gH gene open reading frame (ORF) was 2 505 bp in length and its primary translation product was a polypeptide of 834 amino acids long. It possessed several characteristics of membrane glycoproteins, including an N-terminal hydrophobic signal sequence, an external domain containing eight putative N-linked glycosylation sites, a C-terminal transmembrane domain, and a charged cytoplasmic tail. Comparison with other herpesvirus revealed identities of 20.2, 25.1, 23.0, 23.0, 26.5 and 26.0% with the gH counterparts of the human herpesvirus virus 1 (HSV1), equine herpesvirus 4 (EHV4), bovine herpesvirus 1 (BHV1), pseudorabies virus (PRV), gallid herpesvirus 2 (GHV2) and gallid herpesvirus 3 (GHV3), respectively.展开更多
The DNA of duck plague virus (DPV) thymidine kinase (TK) gene was cloned and sequenced from a vaccine virus in the study. Degenerate oligonucleotide primers for the consensus site of herpesvirus UL24, TK, and glyc...The DNA of duck plague virus (DPV) thymidine kinase (TK) gene was cloned and sequenced from a vaccine virus in the study. Degenerate oligonucleotide primers for the consensus site of herpesvirus UL24, TK, and glycoprotein H(gH) gene were used in the polymerase chain reaction (PCR) to amplify DNA product with 3 741-base-pairs (bp) in size. DNA sequence analysis revealed a 1 077-base-pairs (bp) open reading frame (ORF) encoding a 358 amino acid polypeptide homologous to herpesvirus TK proteins. The predicted TK protein shared 31.2, 41.3, 35.7, 37.4, and 28.4% identity with herpes simplex virus typel, equine herpesvirus type 4, Marek's disease virus 2, herpesvirus turkey, and infectious laryngotracheitis virus, respectively. Comparison of the amino acid sequences of other herpesvirus TK proteins showed that these proteins were not conserved on the whole, otherwise the portion of the TK proteins corresponding to the nucleotide binding domain and the nucleoside binding site were highly conserved among herpesvirus. Comparison with the amino acid sequences of the conserved nucleotide and nucleoside binding domains of other eleven herpesvirus TK proteins to the predicted DPV peptide confirmed its identity as the DPV TK protein.展开更多
Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, ...Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the duck plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-AgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HJTrapTM HeparJn HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-△gC-EGFP or wild-type strain Chinese virulent duck plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on Viral adsorption is independent of interactions between gC and heparin sulfate on cell surface. All in all, this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cells. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during duck plague virus infection.展开更多
The purpose of the paper was to assess the frequency of secondary transmissions of primary pneumonic plague relative to the onset of fever.A simple backcalculation method was employed to estimate the frequency of seco...The purpose of the paper was to assess the frequency of secondary transmissions of primary pneumonic plague relative to the onset of fever.A simple backcalculation method was employed to estimate the frequency of secondary transmissions relative to disease-age.A likelihood-based procedure was taken using observed distributions of the serial interval(n = 177) and incubation period(n = 126).Furthermore,an extended model was developed to account for the survival probability of cases.The simple backcalculation suggested that 31. 0%(95%confidence intervals(CI):11.6,50.4) and 28.0%(95%CI:10.2,45.8) of the total number of secondary transmissions had occurred at second and third days of the disease,respectively,and more than four-fifths of the secondary transmission occurred before the end of third day of disease.The survivorship-adjusted frequency of secondary transmissions was obtained,demonstrating that the infectiousness in later stages of illness was not insignificant and indicates that the obtained frequencies were likely biased on underlying factors including isolation measures.In conclusion,the simple exercise suggests a need to implement countermeasures during pre-clinical stage or immediately after onset.Further information is needed to elucidate the finer details of the disease-age specific infectiousness.展开更多
Objective LcrV is an important component for the development of a subunit vaccine against plague.To reduce immunosuppressive activity of LcrV,a recombinant LcrV variant lacking amino acids 271 to 326 (rV270) was pre...Objective LcrV is an important component for the development of a subunit vaccine against plague.To reduce immunosuppressive activity of LcrV,a recombinant LcrV variant lacking amino acids 271 to 326 (rV270) was prepared by different methods in this study.Methods A new strategy that produced non-tagged or authentic rV270 protein was designed by insertion of rV270-thrombin-hexahistidine fusion gene into the vector pET24a,or by insertion of hexahistidine-enterokinase-rV270 or hexahistitine-factor Xa-rV270 fusion gene into the vector pET32a.After Co2+ affinity chromatography,a purification strategy was developed by cleavage of His tag on column,following Sephacryl S-200HR column filtration chromatography.Results Removal of His tag by thrombin,enterokinase and factor Xa displayed a yield of 99.5%,32.4% and 15.3%,respectively.Following Sephacryl S-200HR column filtration chromatography,above 97% purity of rV270 protein was obtained.Purified rV270 that was adsorbed to 25% (v/v) Al(OH)3 adjuvant in phosphate-buffered saline (PBS) induced very high titers of antibody to rV270 in BALB/c mice and protected them (100% survival) against subcutaneous challenge with 106 CFU of Y.pestis virulent strain 141.Conclusion The completely authentic rV270 protein can be prepared by using enterokinase or factor Xa,but they exhibited extremely low cleavage activity to the corresponding recognition site.Thrombin cleavage is an efficient strategy to prepare non-tagged rV270 protein and can be easily operated in a large scale due to its relatively low cost and high cleavage efficacy.The recombinant rV270 can be used as a key component to develop a subunit vaccine of plague.展开更多
Objective The current outbreak of Zika virus(ZIKV)poses a severe threat to human health.Two ZIKV strains were isolated from mosquitoes collected from the Dejiang prefecture in China in 2016,which was the first isolati...Objective The current outbreak of Zika virus(ZIKV)poses a severe threat to human health.Two ZIKV strains were isolated from mosquitoes collected from the Dejiang prefecture in China in 2016,which was the first isolation of ZIKV in nature in China.Methods In this study,serum samples were collected from 366 healthy individuals and 104 animals from Dejiang prefecture in 2017,and the plaque reduction neutralization test(PRNT)was used to evaluate the seroprevalence of ZIKV.Results None of the 366 residents from whom the samples were collected were seropositive for ZIKV.None of the 11 pigs from whom the samples were collected were seropositive for ZIKV,while 1 of 63(1.59%)chickens and 2 of 30(6.67%)sheep were seropositive for ZIKV.Conclusions The extremely low seropositivity rate of ZIKV antibodies in animals in the Dejiang prefecture,Guizhou province in this study indicates that ZIKV can infect animals;however,there is a low risk of ZIKV circulating in the local population.展开更多
This study was conducted to define the taxonomic status of Spermophilus in the plague area of Dingbian County in Shaanxi Province,China,through the two-factor variance analysis of morphological characteristics,DNA bar...This study was conducted to define the taxonomic status of Spermophilus in the plague area of Dingbian County in Shaanxi Province,China,through the two-factor variance analysis of morphological characteristics,DNA barcoding,and chromosome karyotype analysis.展开更多
Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control m...Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control measures to prevent the spread of the disease. Therefore, the availability of efficient diagnosis tests is of paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. pestis detection. We constructed a set of LAMP primers, which were used in assays to establish the reaction conditions that would lead to the quick visualization of the results by evaluating the test tube with the naked eye. The primers were specifically designed to target the caf1 gene located on pFra/Tox (pMT), a prototypical plasmid of Y. pestis. The LAMP procedure was performed at 65°C for 45 min in a water bath and allowed for the detection of at least 10 pg of bacterial DNA. Due to its simplicity, specificity, sensitivity and rapidity, the LAMP technique is an additional tool that may be implemented in routine plague diagnoses, especially in emergencies.展开更多
Based on the description of epidemic diseases in the chapter of “The Story of Bianque” in Records of Historian, the author has analyzed and explored the truthfulness of its background data and the possibility of the...Based on the description of epidemic diseases in the chapter of “The Story of Bianque” in Records of Historian, the author has analyzed and explored the truthfulness of its background data and the possibility of the plague spreading at that time from both macro and micro angles. The symptoms of plague including chills formation of some doctrines in Traditional Chinese Medicine (TCM) such as exopathic heat disease doctrine, internal injury of spleen and stomach doctrine and epidemic febrile disease doctrine. The course of exogenous phenomena, the author has explored the effect on the initial medical theory, primitive religion and culture due to the spreading of epidemic diseases from the angles of epidemiology, the theory of images and figures, originated from epidemic diseases was proposed. The author also put forward some opinions on the relationship between epidemic diseases spreading and traditional education of TCM, sustained development of TCM.展开更多
Aims&Scope Asian Pacific Journal of Tropical Mediane(APJTM),aims to set up and provide an academic communicating platform for international physicians,medical scientists,allied health scientists and public health ...Aims&Scope Asian Pacific Journal of Tropical Mediane(APJTM),aims to set up and provide an academic communicating platform for international physicians,medical scientists,allied health scientists and public health workers,especially those of the Asia-展开更多
基金supported by the Fundamental Research Funds for the Central Universities(No.21lzujbkydx036).
文摘Daoist medicine,a special form of traditional Chinese medicine with distinct Daoist styles,has played an important role in the history of plague control in ancient China.Emerged from the pandemics at the end of the Han Dynasty(146 C.E.-220 C.E.),Daoism,the native Chinese religion,has always had a religious mission to end the spread of plagues;thus,it has actively participated in treating plagues through various techniques of medicine and religion.In the practice of plague treatment for thousands of years,Daoist medicine has developed a special etiology of plague that includes Qi-based theories(Qi is considered to be the force that informs and animates all things in Daoism,thus,plague is also considered to be caused by specific and hazardous Qi.)and worm-based theories,forming a strategy of plague control that includes prevention before an epidemic,active treatment during the epidemic,and recovery after the epidemic;these developed plague prevention methods combine isolation,disinfection,and drug use.Additionally,it is the first to practice a primitive immunoprophylaxis method that uses tissues and secretions of infected persons and animals to infect healthy individuals to gain disease immunity.It has formed a plague treatment method mainly based on drug treatment and ritual treatment,supplemented by acupuncture and physical treatment.It has left behind many effective drugs and prescriptions for plague treatment,some of which have a wide clinical application.Hence,Daoist medicine has made great contributions to plague control in Chinese history.Its strategies,methods,medicines,and prescriptions for plague control still have considerable theoretical and application today,especially in the coronavirus disease 2019(COVID-19)pandemic.
文摘Inspired by recent environmental historical studies on animal extinctions and human-animal relations,this paper shifts scholarly attention from the plague-centered narrative of the great Pneumonic Plague Epidemics(1910—11)to the fate of the plague host animals,Tarbagan marmots(Marmota sibirica>,and examines their ncar-extinction in Northwest Manchuria(Hulunbuir)from the 1900s to 1930s.Focusing on changing images of Tarbagan marmots from“inexpensive,”“sacred,”and“beneficial”in the pre-modem period to“valuable,”“dangerous,”and“noxious”in the early twentieth century,it argues that three interrelated factors:the international fur trade,pneumonic plagues,and environment changes together resulted in the“retreat of the marmots.”It also]uses this case study to help us better understand larger historical changes that occurred by contextualizing them in terms of human-marmot relations in Manchuria,China and beyond.
基金funded by the National Social Science Fund of China(No.18ZDA175)。
文摘Plague is one of the severe infectious diseases which has had a huge impact on human society throughout history.Although there is abundant research on the disease,few studies focus on the concept of plague itself.It is generally believed that Shu Yi was first coined by Chinese doctors in the late 19th century,and it was closely related to the introduction of new medicine.This statement is not accurate,because plague was prevalent in Yunnan and Guangdong provinces in the late Qing Dynasty.At that time,people had already recognized the relationship between the outbreak of plague and the death of rats,and gradually named the epidemic disease as Shu Yi.This name was used by literati and doctors by coincidence.As the epidemic became more and more serious,more related works were widely disseminated,and Shu Yi changed from a folk name to a formal name.Later,with the help of the government’s active introduction of modern health and epidemic prevention mechanisms and the implementation of rodent control measures,Shu Yi became more popular and gradually recognized by all walks of society.The emergence and prevalence of Shu Yi created the history of zoonosis in China,and thus formed a new paradigm of naming human epidemics after related animal names.
文摘The Great Plague:The Diary of Alice Paynton,London 1665-1666 adopts the realistic painting style and direct writing to the painful experience,describes the situation of the Great Plague in London truly from the perspective of children,and breaks people’s cognitive expectation of children’s literature,the construction of children’s Gray inner world is completed behind the true description of the Great Plague in London with the true brushwork,which presents a different world of childlike innocence.Despite the importance of epidemics writing in literature,the child’s diary perspective of them is one of the least developed of Plague Narrative.Only when authenticity and the child’s grey interior are integrated will we reveal a true picture of society as it was.
文摘Chi Zijian’s novel White Snow Crow is about the pestis in Northeast China from the autumn and winter of 1910 to the spring of 1911,focusing on the living conditions of people in Fujiadian,Harbin,under the shadow of the pestis,and thus connects the vicissitudes of Harbin in the development of modern Chinese history.Re-reading this novel in the context of the post-epidemic era not only allows us to immerse ourselves in the specific temporal and spatial fields described in the text with an immersive reading mindset,feel the heavy impact that the disaster has brought to the people of Northeast China,but also provide us with a different perspective to observe the current social reality.In particular,the social problems shown by the novel through the pestis and the description of ordinary people’s life experience under the plague still deserve further discussion.
文摘Season of the Witch is one of the great productions with all these characteristics which were introduced during August around China.The story was told during the final historical period of the Crusades.The Crusades were a series of religious wars.The crusaders comprised military units from all over Western Europe.During 14th century Black Plague was threatened the Whole Europe.The Plague destroyed the Crusade and each town.People began to suspect some women which abused as witches should be take responsibilities.They put some women to death.The movie not only tell us a story,but lead us to find out lights from the dark world during the black age.
基金Supported by 2011 Special Projects in Scientific Research of TCM Profession(201107006)2018 Preventive Treatment of Disease in Tianjin University of Traditional Chinese Medicine(XJ201801)。
文摘In view of the serious threat to people's health caused by the Coronavirus Disease 2019(COVID-19),according to the relevant spirit of the Notice on Establishing the Cooperation Mechanism of traditional Chinese and Western Medicine in the Prevention and Treatment of COVID-19 and other Infectious Diseases issued by the National Health Commission of the People's Republic of China,National Administration of Traditional Chinese Medicine of the People's Republic of China,on the basis of our early clinical research of using press needle embedding(intradermal acupuncture)to prevent and treat related diseases,the symptoms,etiology and pathogenesis of the disease were analyzed.We try to use the simple and easy intradermal acupuncture therapy to prevent and assist the treatment of the disease and its complications,hoping to make a modest contribution to help patients relieve part of suffering,and also provide advice for the doctors in the treatment of the disease.
文摘The glycoprotein H (gH) gene homologue of duck plague virus (DPV) was cloned by degenerate polymerase chain reaction (PCR) and sequenced. It was located immediately downstream from the thymidine kinase gene (TK). In addition, the 3'-end of the gene homologue to herpesvirus UL21 was located downstream from the gH gene. DPV gH gene open reading frame (ORF) was 2 505 bp in length and its primary translation product was a polypeptide of 834 amino acids long. It possessed several characteristics of membrane glycoproteins, including an N-terminal hydrophobic signal sequence, an external domain containing eight putative N-linked glycosylation sites, a C-terminal transmembrane domain, and a charged cytoplasmic tail. Comparison with other herpesvirus revealed identities of 20.2, 25.1, 23.0, 23.0, 26.5 and 26.0% with the gH counterparts of the human herpesvirus virus 1 (HSV1), equine herpesvirus 4 (EHV4), bovine herpesvirus 1 (BHV1), pseudorabies virus (PRV), gallid herpesvirus 2 (GHV2) and gallid herpesvirus 3 (GHV3), respectively.
文摘The DNA of duck plague virus (DPV) thymidine kinase (TK) gene was cloned and sequenced from a vaccine virus in the study. Degenerate oligonucleotide primers for the consensus site of herpesvirus UL24, TK, and glycoprotein H(gH) gene were used in the polymerase chain reaction (PCR) to amplify DNA product with 3 741-base-pairs (bp) in size. DNA sequence analysis revealed a 1 077-base-pairs (bp) open reading frame (ORF) encoding a 358 amino acid polypeptide homologous to herpesvirus TK proteins. The predicted TK protein shared 31.2, 41.3, 35.7, 37.4, and 28.4% identity with herpes simplex virus typel, equine herpesvirus type 4, Marek's disease virus 2, herpesvirus turkey, and infectious laryngotracheitis virus, respectively. Comparison of the amino acid sequences of other herpesvirus TK proteins showed that these proteins were not conserved on the whole, otherwise the portion of the TK proteins corresponding to the nucleotide binding domain and the nucleoside binding site were highly conserved among herpesvirus. Comparison with the amino acid sequences of the conserved nucleotide and nucleoside binding domains of other eleven herpesvirus TK proteins to the predicted DPV peptide confirmed its identity as the DPV TK protein.
基金supported by the grants from the National Natural Science Foundation of China(31072157)the National Key Technologies R&D Program of China during the 12th Five-Year Plan period(2015BAD12B05)+1 种基金the Foundation of China Agricultural Research System(CARS-43-8)the Major Project of Education Department in Sichuan,China(16ZA0027)
文摘Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the duck plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-AgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HJTrapTM HeparJn HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-△gC-EGFP or wild-type strain Chinese virulent duck plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on Viral adsorption is independent of interactions between gC and heparin sulfate on cell surface. All in all, this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cells. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during duck plague virus infection.
文摘The purpose of the paper was to assess the frequency of secondary transmissions of primary pneumonic plague relative to the onset of fever.A simple backcalculation method was employed to estimate the frequency of secondary transmissions relative to disease-age.A likelihood-based procedure was taken using observed distributions of the serial interval(n = 177) and incubation period(n = 126).Furthermore,an extended model was developed to account for the survival probability of cases.The simple backcalculation suggested that 31. 0%(95%confidence intervals(CI):11.6,50.4) and 28.0%(95%CI:10.2,45.8) of the total number of secondary transmissions had occurred at second and third days of the disease,respectively,and more than four-fifths of the secondary transmission occurred before the end of third day of disease.The survivorship-adjusted frequency of secondary transmissions was obtained,demonstrating that the infectiousness in later stages of illness was not insignificant and indicates that the obtained frequencies were likely biased on underlying factors including isolation measures.In conclusion,the simple exercise suggests a need to implement countermeasures during pre-clinical stage or immediately after onset.Further information is needed to elucidate the finer details of the disease-age specific infectiousness.
基金supported by the National Key Program for Infectious Diseases of China (2009ZX10004-4001)
文摘Objective LcrV is an important component for the development of a subunit vaccine against plague.To reduce immunosuppressive activity of LcrV,a recombinant LcrV variant lacking amino acids 271 to 326 (rV270) was prepared by different methods in this study.Methods A new strategy that produced non-tagged or authentic rV270 protein was designed by insertion of rV270-thrombin-hexahistidine fusion gene into the vector pET24a,or by insertion of hexahistidine-enterokinase-rV270 or hexahistitine-factor Xa-rV270 fusion gene into the vector pET32a.After Co2+ affinity chromatography,a purification strategy was developed by cleavage of His tag on column,following Sephacryl S-200HR column filtration chromatography.Results Removal of His tag by thrombin,enterokinase and factor Xa displayed a yield of 99.5%,32.4% and 15.3%,respectively.Following Sephacryl S-200HR column filtration chromatography,above 97% purity of rV270 protein was obtained.Purified rV270 that was adsorbed to 25% (v/v) Al(OH)3 adjuvant in phosphate-buffered saline (PBS) induced very high titers of antibody to rV270 in BALB/c mice and protected them (100% survival) against subcutaneous challenge with 106 CFU of Y.pestis virulent strain 141.Conclusion The completely authentic rV270 protein can be prepared by using enterokinase or factor Xa,but they exhibited extremely low cleavage activity to the corresponding recognition site.Thrombin cleavage is an efficient strategy to prepare non-tagged rV270 protein and can be easily operated in a large scale due to its relatively low cost and high cleavage efficacy.The recombinant rV270 can be used as a key component to develop a subunit vaccine of plague.
基金supported by the National Science and Technology Major Project [2018ZX10713-002,2017ZX10104001,2018ZX10711001]National key research and development project [2017YFC1200505,2016YFC1200905]the Development Grant of State Key Laboratory of Infectious Disease Prevention and Control [2015SKLID505,2014SKLID103]
文摘Objective The current outbreak of Zika virus(ZIKV)poses a severe threat to human health.Two ZIKV strains were isolated from mosquitoes collected from the Dejiang prefecture in China in 2016,which was the first isolation of ZIKV in nature in China.Methods In this study,serum samples were collected from 366 healthy individuals and 104 animals from Dejiang prefecture in 2017,and the plaque reduction neutralization test(PRNT)was used to evaluate the seroprevalence of ZIKV.Results None of the 366 residents from whom the samples were collected were seropositive for ZIKV.None of the 11 pigs from whom the samples were collected were seropositive for ZIKV,while 1 of 63(1.59%)chickens and 2 of 30(6.67%)sheep were seropositive for ZIKV.Conclusions The extremely low seropositivity rate of ZIKV antibodies in animals in the Dejiang prefecture,Guizhou province in this study indicates that ZIKV can infect animals;however,there is a low risk of ZIKV circulating in the local population.
基金supported by the grant of the Science and Technology Research and Development of Shaanxi Province[No.2012K16-12-03]
文摘This study was conducted to define the taxonomic status of Spermophilus in the plague area of Dingbian County in Shaanxi Province,China,through the two-factor variance analysis of morphological characteristics,DNA barcoding,and chromosome karyotype analysis.
文摘Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control measures to prevent the spread of the disease. Therefore, the availability of efficient diagnosis tests is of paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. pestis detection. We constructed a set of LAMP primers, which were used in assays to establish the reaction conditions that would lead to the quick visualization of the results by evaluating the test tube with the naked eye. The primers were specifically designed to target the caf1 gene located on pFra/Tox (pMT), a prototypical plasmid of Y. pestis. The LAMP procedure was performed at 65°C for 45 min in a water bath and allowed for the detection of at least 10 pg of bacterial DNA. Due to its simplicity, specificity, sensitivity and rapidity, the LAMP technique is an additional tool that may be implemented in routine plague diagnoses, especially in emergencies.
文摘Based on the description of epidemic diseases in the chapter of “The Story of Bianque” in Records of Historian, the author has analyzed and explored the truthfulness of its background data and the possibility of the plague spreading at that time from both macro and micro angles. The symptoms of plague including chills formation of some doctrines in Traditional Chinese Medicine (TCM) such as exopathic heat disease doctrine, internal injury of spleen and stomach doctrine and epidemic febrile disease doctrine. The course of exogenous phenomena, the author has explored the effect on the initial medical theory, primitive religion and culture due to the spreading of epidemic diseases from the angles of epidemiology, the theory of images and figures, originated from epidemic diseases was proposed. The author also put forward some opinions on the relationship between epidemic diseases spreading and traditional education of TCM, sustained development of TCM.
文摘Aims&Scope Asian Pacific Journal of Tropical Mediane(APJTM),aims to set up and provide an academic communicating platform for international physicians,medical scientists,allied health scientists and public health workers,especially those of the Asia-