Technical Regulations for Planting of Forage Sorghum in Hebei Province

According to the summary of many years of experiments, the application scope, basic demands, preparation before seeding, seeding demands, field management, mowing and harvesting, and other technical requirements of technical regulations for planting of forage sweet sorghum were studied, and specific measures and technical indicators of the technical regulations were analyzed, aiming to provide normalized, standardized, industrial and marketization technical support for the planting of forage sweet sorghum.

Effect of microenvironmental quantitative regulation on growth of Korean pine trees planted under secondary forest

Korean pine（Pinus koraiensis） and broadleaved mixed forest in Northeast China has been changed regressively into secondary forest with almost no conifers.Planting Korean pine trees under the canopy of secondary forest is a feasible approach for recovering Korean pine and broadleaved mixed forest.For establishing an effective growth promotion method for under-canopy planted young Korean pine trees,two stands were selected as the experiment plots,Stand A（planted in 1989） and Stand B（planted in 1982）,and an experiment of microenvironment regulation was conducted relying mainly on Opening degree（K=1,K=1.5,K=2,CK） in 2004.The results were shown that the adjustment had promoted growth of diameter and height of Korean pine planted in Stand A and Stand B,and had a significant influence on the growth rate of basal diameter,diameter at breast height and height in the two growth stands.The four years periodic increment of mean diameter and height of Korean pine planted in 1989 and in 1982 after regulation in K=1 level were 63.4%（D0） and 82.7%（H）,64.8%（D1.3） and 69.7%（H） higher than that of control respectively.Quantitative regulation had significant influence on specific leaf area of Korean pine planted in 1989,and the current year specific leaf area（SLA） was lager than perennial year SLA.Quality indexes of natural pruning capacity,normal form quotient and crown size was not significantly changed but shown a positive tendency.The regulation scheme of Opening degree K=1 might be proper for adjusting the microenvironment of Korean pine trees planted under the canopy of secondary forest when the Korean pine trees were in the growth period of 15 to 26 years old in the experiment region.

Technical Regulations for Standardized Planting of Rare and Multi-purpose Wild Fruit Kadsura coccinea

The cultivation technology were standardized for improving the yield and quality,and the commodity value and the competitiveness of fruit market,and solving the bottleneck problem—fruit quality and yield that affect the industrial development of rare and multi-purpose wild fruit Kadsura coccinea(Lem.)A.C.Smith.Through the investigation of K.coccinea production area and planting area,collection and analysis of fruit samples,it was found that in most planting area,the varieties of K.coccinea were mixed,with great differences in fruit quality and yield,which makes it impossible to realize large-scale planting.In view of these problems,a series of studies have been carried out and the technical regulations for standardized planting of rare and multi-purpose wild fruit K.coccinea were formulated.

Novel Synthesis, Crystal Structure, and Plant-growth Regulation Activity of(1S,4R)-4,7,7-Trimethyl-6-oxabicyclo[3.2.1]octane-1,4-diol

The title compound,（1S,4R）-4,7,7-trimethyl-6-oxabicyclo [3.2.1] octane-1,4-diol（C（10）H（18）O3）, has been synthesized from terpinolene via one-step catalytic synthetic method and structurally characterized by means of HRMS, IR, 1H-NMR, （13）C-NMR and single-crystal X-ray diffraction. The compound crystallizes in trigonal, space group R-3, with a = 27.892（9）, b = 27.892（9）, c = 6.720（2） A, γ = 120°, Z = 18, V = 4527（3） A3, Dc = 1.230 g/cm3, Mr = 186.24, λ（Mo Kα） = 0.71073？, μ = 0.09 mm（-1）, F（000） = 1836, the final R = 0.051 and wR = 0.161. The title compound molecule contained a 6-oxabicyclo[3.2.1]octane skeleton and two hydroxyl groups, which were connected through intermolecular O–H…O hydrogen bonds to generate a two-dimensional network. Especially, the preliminary bioassay showed that the title compound can promote the root growth and shoot elongation of rape（Brassica campestris） at low concentration（0.62570 mmol·L^-1） and inhibit them at high concentration（〉 70 mmol·L^-1）.

Research Progress in Plant Invertase

Invertase is a key enzyme in sucrose catabolism and crucial for plant assimilate distribution. With the development of molecularbiology, a lot of invertsae genes were cloned recently, and significant progress have been made in regulators on the expression of invertase genes.Thus, this article summarized theresearch progress of invertase in biological characteristics, molecular characteristics and expression regulation.

Technical Regulations for Planting of Forage Millet Rich in Selenium in Hebei Province

According to the summary of many years of experiments, the application scope, basic demands, preparation before seeding, seeding demands, field management, mowing, transportation, storage, ledger management and other technical requirements of technical regulations for planting of forage millet rich in selenium were studied, and specific measures and technical indicators of the technical regulations were analyzed, aiming to provide normalized, standardized, industrial and marketization technical support for the planting of forage millet rich in selenium.

Mechanism of Primary Frequency Regulation for Battery Hybridization in Hydropower Plant

Battery hybridization in hydropower plants is a hydropower flexibility enhancement technology innovation that can potentially expand hydropower’s contributions to the grid,but its fundamental characteristics and influencing mechanisms are still unclear.In this paper,primary frequency regulation(PFR)performance and the mechanism of this new technology are studied.A battery hybridized hydropower plant(BH-HPP)model,based on a field-measured-data-based hydropower plant(HPP)model and a verified battery simplified model,is established.Analysis of system stability and dynamics is undertaken for three different battery control strategies by root locus and participation factor methods.Compared to conventional HPPs,analysis results theoretically reveal BH-HPP can not only accelerate system regulation rapidity but also effectively enlarge HPP stability region during PFR process.Time domain simulation verifies the results and further shows synthetic control has better performance among introduced strategies.Besides,initial design ranges of control parameters considering battery capacity and a renewable energy source scenario case are also discussed.This work could provide theoretical support for flexibility enhancement solutions for hydropower systems.

Generic load regulation strategy for enhancing energy efficiency of chiller plants

In many chiller plants,high coefficient of performance(COP)is only achieved at a few favorable part load ratios(PLRs),while the COP is low at many other non-favorable PLRs.To address this issue,this study proposes a generic load regulation strategy that aims to maintain chiller plants operating at high COP,particularly under non-favorable PLRs.This is achieved by incorporating thermal energy storage(TES)units and timely optimizing the charging and discharging power of the integrated TES units.The optimal charging and discharging power is determined by solving a dynamic optimization problem,taking into account the performance constraints of the TES units and the chiller plants.To provide an overview of the energy-saving potential of the proposed strategy,a comprehensive analysis was conducted,considering factors such as building load profiles,COP/PLR curves of chillers,and attributes of the TES units.The analysis revealed that the proposed load regulation strategy has the potential to achieve energy savings ranging from 5.7%to 10.8%for chiller plants with poor COPs under unfavorable PLRs,particularly in buildings with significant load variations.

Regulations on Planting Techniques of Harmless Feeding Sorghum bicolor(L.) Moench in Hebei

According to many years of experimental summary, regulations on planting technique of harmless feeding Sorghum bicolor （L.） Moenchwere studied from the application scope, basic demands of planting, preparation be fore seeding, demands of seeding, field management, clipping and harvesting, transportation, storage, ledger management and other technical requirements, and specific measures and technical indicators of the technical regulations were analyzed, so as to provide normalized, standardized, industrial and marketization technical support for the planting of harmless feeding S. bicolor （L.） Moench.

Post-Translational Derepression of Invertase Activity in Source Leaves via Down-Regulation of Invertase Inhibitor Expression Is Part of the Plant Defense Response

There is increasing evidence that pathogens do not only elicit direct defense responses, but also cause pronounced changes in primary carbohydrate metabolism. Cell-wall-bound invertases belong to the key regulators of carbohydrate partitioning and source-sink relations. Whereas studies have focused so far only on the transcriptional induction of invertase genes in response to pathogen infection, the role of post-translational regulation of invertase activity has been neglected and was the focus of the present study. Expression analyses revealed that the high mRNA level of one out of three proteinaceous invertase inhibitors in source leaves of Arabidopsis thaliana is strongly repressed upon infection by a virulent strain of Pseudomonas syringae pv. tomato DC3000. This repression is paralleled by a decrease in invertase inhibitor activity. The physiological role of this regulatory mechanism is revealed by the finding that in situ invertase activity was detectable only upon infection by P. syringae. In contrast, a high invertase activity could be measured in vitro in crude and cell wall extracts prepared from both infected and non-infected leaves. The discrepancy between the in situ and in vitro invertase activity of control leaves and the high in situ invertase activity in infected leaves can be explained by the pathogen-dependent repression of invertase inhibitor expression and a concomitant reduction in invertase inhibitor activity. The functional importance of the release of invertase from post-translational inhibition for the defense response was substantiated by the application of the competitive chemical invertase inhibitor acarbose. Posttranslational inhibition of extracellular invertase activity by infiltration of acarbose in leaves was shown to increase the susceptibility to P. syringae. The impact of invertase inhibition on spatial and temporal dynamics of the repression of photosynthesis and promotion of bacterial growth during pathogen infection supports a role for extracellular invertase in plant defense. The acarbose-mediated increase in susceptibility was also detectable in sid2 and cpr6 mutants and resulted in slightly elevated levels of salicylic acid, demonstrating that the effect is independent of the salicylic acid-regulated defense pathway. These findings provide an explanation for high extractable invertase activity found in source leaves that is kept inhibited in situ by post-translational interaction between invertase and the invertase inhibitor proteins. Upon pathogen infection, the invertase activity is released by repression of invertase inhibitor expression, thus linking the local induction of sink strength to the plant defense response.

Organ Abscission in Plants: With Special Emphasis on Bell Pepper

Bell pepper(Capsicum annuum L.),along with potato and tomato,is one of the three most cultivated vegetables in the world.Bell pepper is worldwide accepted due to its characteristics of color,smell,flavor,and texture.Bell pepper is also considered a nutritious food due to its vitamin and antioxidant contents.In Mexico,bell pepper production has a high value because it is grown for the international markets,particularly the United States of America.Nevertheless,the abscission offlowers and fruits is a factor that limits the yield,hinders the planning of activities,and causes a variation in the prices of peppers.Due to the importance of this process,in this work we did a bibliometric analysis and literature review of scientific advances for the understanding of the abscission pro-cess in plants,and in particular for the bell pepper.Finally,we introduce new perspectives that would help direct future research about organ abscission in plants.Likewise,the lack of research that would further clarify abscis-sion process in the bell pepper plant is discussed.

Regulations and Practice on Flue Gas Denitrification for Coal-Fired Power Plants in China

In China, according to the relative up-to-date regulations and standards, the maincontrol measure for NOX emission of coal-fired power plants is, in principle, low NOXcombustion. However, in recent years, more and more newlyapproved coal-fired plantswere required to install flue gas denitrification equipment. This article expounds if fluegas denitrification is necessary from several aspects, including constitution of NOX, itsimpact to environment, operation ofdeNOXequipment in USA, as wellas the differencein ambient air quality standard between China and World Health Organization. It setsforth themes in urgent need of study and areas where deNOX equipment is necessaryfor new projects, besides a recommendation that the emission standards for thermalpowerplants should be revised as soon as possible in China.

Study of Effects of Plant Growth Regulators on Fruit Quality of Table Grape

The effects of different treatments on the seedlessness and fruit quality of‘Miguang’table grape was studied by using plant growth regulators,gibberellin acid(GA 3)and forchlorfenuron(CPPU),under different concentrations and application time.The results showed that the effects of different treatments on the seedlessness and fruit quality were different.Seedless rate,cluster weight,berry weight,berry shape index,soluble solid content,total acid content,soluble solids to acidity ratio,pulling resistance,turgor pressure and flesh firmness without skin were comprehensively evaluated,as a result of which,the optimum treatment on‘Miguang’table grape was to apply with GA 320 mg/L+SM 200 mg/L at one week before bloom and GA 325 mg/L+CPPU 3 mg/L at two weeks after bloom.

The Effect of Plant Growth Regulators on Physico-Chemical Properties of Safflower (Carthamus tinctorius L.) Derived Biodiesel

Global concerns about the environmental impact of combustion emissions from petroleum fuels influence new research to seek for alternative energy sources. The current study investigates the possibility of using safflower (Carthamus tinctorius L.) as an alternative biodiesel raw material. Four plant growth regulators (PGR) were used to boost the production of safflower. Thirteen treatments were constituted from the four plant regulators and applied to the safflower crop arranged in completely randomised design, repeated three times. The results show that the effect of plant growth regulators was not more than that of the control. More studies have to be channelled towards the relationship between safflower and plant growth regulators.

Thansgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system

After pre-culture and treatment of osmosis, cotyledons of immature peanut (Arachis hypogaea L.) zygotic embryos were transformed via particle bombardment with a plasmid containing a chimeric hph gene conferring resistance to hygromycin and a chimeric intron-gus gene. Selection for hygromycin resistant calluses and somatic embryos was initiated at 10th d post-bombardment on medium containing 10-25 mg/L hygromycin. Under continuous selection, hygromycin resistant plantlets were regenerated from somatic embryos and were recovered from nearly 1.6% of the bombarded cotyledons. The presence and integration of foreign DNA in regenerated hygromycin resistant plants was confirmed by PCR (polymerase chain reaction) for the intron-gus gene and by Southern hybridization of the hph gene. GUS enzyme activity was detected in leaflets from transgenic plants but not from control, non-transformed plants. The production of transgenic plants are mainly based on a newly improved somatic embryogenesis regeneration system developed by us.

Influencing in vitro clonal propagation of Chonemorpha fragrans(moon) Alston by culture media strength,plant growth regulators,carbon source and photo periodic incubation

Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.

Functions of Plant Growth Substances in the Growth of Dendrobium officinale Kimura et Migo

[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substance on the growth and development of D. officinale Kimura et Migo. [Results] Plant growth regulators play an important role in the process of tissue culture rapid propagation of D. officinale Kimura et Migo,and the main regulatory pathways are as follows.( i) Plant growth regulators promote the growth and development of D. officinale Kimura et Migo through regulating the changes of its endogenous hormones.( ii) Plant growth regulators affect the accumulation of polysaccharide content in D. officinale Kimura et Migo through regulating the changes in chlorophyll content and enzymes involved in sucrose metabolism.( iii) Plant growth regulators can increase the activity of antioxidant enzymes of D. officinale Kimura et Migo,to increase its stress resistance. [Conclusions]Using molecular biology techniques,combined with the changes of plant hormones in D. officinale Kimura et Migo,and analysis on key enzyme in the synthesis of plant hormones and gene expression,it is feasible to study the rules o changes in the content of active content of D. officinale Kimura et Migo.

Multilayered regulation of secondary metabolism in medicinal plants

Medicinal plants represent a huge reservoir of secondary metabolites(SMs),substances with significant pharmaceutical and industrial potential.However,obtaining secondary metabolites remains a challenge due to their low-yield accumulation in medicinal plants;moreover,these secondary metabolites are produced through tightly coordinated pathways involving many spatiotemporally and environmentally regulated steps.The first regulatory layer involves a complex network of transcription factors;a second,more recently discovered layer of complexity in the regulation of SMs is epigenetic modification,such as DNA methylation,histone modification and small RNA-based mechanisms,which can jointly or separately influence secondary metabolites by regulating gene expression.Here,we summarize the findings in the fields of genetic and epigenetic regulation with a special emphasis on SMs in medicinal plants,providing a new perspective on the multiple layers of regulation of gene expression.

Influence of Plant Growth Regulators on Pollen Germination and Growth of Xinjiang Apricots

[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in solid media supplemented with five plant growth regulators （GA3 , NAA, 2, 4-D, 6-BA, IAA）. Then the rate of pollen germination and the length of pollen tube were respectively measured. [Result] In a certain concentration range, GA3 most significantly promoted the pollen germination and the pollen tube growth of Shushanggan, Kalayulvke, Dayoujia, Yiliakeyulvke and Kabakehuanna; NNA had the strongest improvement function on Kumaiti’s pollen germination and pollen tube growth. [Conclusion] All the five plant growth regulators promoted the pollen germination and the pollen tube growth of apricots at low concentration but inhibited them at high concentration.

Effects of Plant Growth Regulator on Absorption,Circulation and Contents of Potassium in Flue-cured Tobacco

[Objective] This study was to investigate the effects of plant growth regulator on accumulation and circulation of potassium in flue-cured tobacco.[Methods] Hydroponics experiment was adopted to study the effects of plant growth regulator（NAA,GA3,BR） on accumulation and circulation of potassium in flue-cured tobacco.[Results] The BR treatment increased the absorption of potassium,calcium and magnesium in flue-cured tobacco,reduced the potassium emission from the root,enhanced the accumulation and contents and promoted the circulation of potassium in the tobacco plants.There was no statistical difference between the NAA and GA3 treatment.And BR treatment was 0.52%,0.30%,0.28% higher than NAA treatment in enhancing potassium content in tobacco plants at 2,7,12 d after topping.In a word,the results showed that BR treatment was the most.[Conclusion] BR treatment could effectively enhance potassium content in tobacco plants after topping.