Somatic Embryogenesis and Plantlet Regeneration in Callus Cultures Derived from Mature Zygotic Embryos of Slash Pine

White, translucent, and mucilaginous embryogenic callus was initiated in cultured mature zygotic embryoexplants of two different seed sources of slash pine(Pinus elliottii) on several culture media containing auxin and cytokinin.Somatic proembryos were induced on media containing 2,4-D and BA. Maturatiol1 was successfully achieved on mediumsupplemented with ABA. Somatic embryos germinated into regeneration plantlets On DCR medium containing activated charcoal. HiStological observatiol1 and scanning electron inicroscopic observatiol1 showed that proembryos derived from em-bryonal suspensor mass(ESM) were formed on the surface or the inside of embryogenic callus, and the prolitbration of pro-embryos was mainly from clcavage polyembryos.

大岩桐叶柄诱导再生植株

用大岩桐叶柄作为外植体,分别对试管苗诱导、增殖和生根条件进行了初步研究。结果表明:适宜诱导培养基以MS+6-BA2 0mg·L-1+NAA0 2mg·L-1+3%蔗糖+0 7%琼脂最佳,35d其诱导率为1 324,增殖培养基以MS+6-BA2 0mg·L-1+NAA0 1mg·L-1+3%蔗糖+0 7%琼脂为最好,30d其增殖倍数可达5 3倍。生根培养基以1/2MS+NAA0 6mg·L-1+3%蔗糖+0 7%琼脂为最好,生根率可达89 7%。

木薯种质资源离体保存技术研究

为探讨木薯（Manihot esculenta Crantz）种质资源的离体保存方法,以GR891木薯品种试管苗为试材,研究了植物生长抑制剂（PP333、B9、S3307、ABA）对试管苗在常温（25℃）条件下的保存效果。结果表明,在培养室温度（25±2）℃、光照度1500～2 000 lx、光照时间12 h/d的条件下,培养基中加入PP333、B9和S3307均可提高木薯试管苗的存活率。在MS＋6-BA 0.2 mg/L＋NAA 0.1 mg/L＋蔗糖30 g/L＋琼脂6.5 g/L培养基上,木薯常温保存的适宜PP333质量浓度为1.0～1.5 mg/L或B9和S3307质量浓度均为0.5～1.0 mg/L,保存240 d时存活率超过90%,试管苗根系发达、叶色浓绿、生长健壮,0.2～2.0 mg/L ABA培养基上试管苗长势和存活率都明显低于CK（对照）。