The degradation of Escherichia coli bacteria by treatment with cold, weakly ionised, highly dissociated oxygen plasma, with an electron temperature of 3 eV, a plasma density of 8 × 10^15 m^-3 and a neutral oxygen...The degradation of Escherichia coli bacteria by treatment with cold, weakly ionised, highly dissociated oxygen plasma, with an electron temperature of 3 eV, a plasma density of 8 × 10^15 m^-3 and a neutral oxygen atom density of 3.5 × 10^21 m^-3 was studied. To determine the 'real' plasma effects, two methods were used for evaluation and determination, as well as a comparison of the number of bacteria that had survived: the standard plate count technique (PCT) and advanced fluorescence-activated cell sorting (FACS). Bacteria were deposited onto glass substrates and kept below 50 ℃ during the experiments with oxygen plasma. The results showed that the bacteria had fully degraded after about 2 min of plasma treatment, depending slightly on the amount of bacteria that had been deposited on the substrates. The very precise determination of the O flux on the substrates and the two-method comparison allowed for the determination of the critical dose of oxygen atoms required for the destruction of a bacterial cell wall--about 6 × 10^24 m^-2--as well as deactivation of the substrates--about 8 × 10^25 m^-2. These results were taken in order to discuss other results obtained by comparable studies and scientific method evaluations in the determination of plasma effects on bacteria.展开更多
Cast Mg-6 wt pct Al alloy solution-treated at 683 K for 16 h and aged at 498 K was coated by plasma electrolytic oxidation (PEO) method.The Mg-6 wt pct Al alloy aged for 16 h exhibited the highest microhardness and ...Cast Mg-6 wt pct Al alloy solution-treated at 683 K for 16 h and aged at 498 K was coated by plasma electrolytic oxidation (PEO) method.The Mg-6 wt pct Al alloy aged for 16 h exhibited the highest microhardness and wear resistance.After PEO coating,however,the microhardness and wear resistance of coatings on Mg- 6 wt pct Al alloy showed a tendency to decrease with increasing aging time,which was in aggrement with the change of thickness with aging time.In addition,the coatings on solution-treated Mg-6 wt pct Al alloy had better microhardness and wear resistance than those on aged Mg-6 wt pct Al alloys.Consequently,it can be understood that the aging treatment has a deleterious influence on the mechanical properties of coatings on Mg-6 wt pct Al alloy.展开更多
基金The financial support from the Slovenian Research Agency (ARRS)NATO CLG/SPS.984555+1 种基金EU COST grant MP1101the MESS 171037 and 41011 projects for partial support
文摘The degradation of Escherichia coli bacteria by treatment with cold, weakly ionised, highly dissociated oxygen plasma, with an electron temperature of 3 eV, a plasma density of 8 × 10^15 m^-3 and a neutral oxygen atom density of 3.5 × 10^21 m^-3 was studied. To determine the 'real' plasma effects, two methods were used for evaluation and determination, as well as a comparison of the number of bacteria that had survived: the standard plate count technique (PCT) and advanced fluorescence-activated cell sorting (FACS). Bacteria were deposited onto glass substrates and kept below 50 ℃ during the experiments with oxygen plasma. The results showed that the bacteria had fully degraded after about 2 min of plasma treatment, depending slightly on the amount of bacteria that had been deposited on the substrates. The very precise determination of the O flux on the substrates and the two-method comparison allowed for the determination of the critical dose of oxygen atoms required for the destruction of a bacterial cell wall--about 6 × 10^24 m^-2--as well as deactivation of the substrates--about 8 × 10^25 m^-2. These results were taken in order to discuss other results obtained by comparable studies and scientific method evaluations in the determination of plasma effects on bacteria.
文摘Cast Mg-6 wt pct Al alloy solution-treated at 683 K for 16 h and aged at 498 K was coated by plasma electrolytic oxidation (PEO) method.The Mg-6 wt pct Al alloy aged for 16 h exhibited the highest microhardness and wear resistance.After PEO coating,however,the microhardness and wear resistance of coatings on Mg- 6 wt pct Al alloy showed a tendency to decrease with increasing aging time,which was in aggrement with the change of thickness with aging time.In addition,the coatings on solution-treated Mg-6 wt pct Al alloy had better microhardness and wear resistance than those on aged Mg-6 wt pct Al alloys.Consequently,it can be understood that the aging treatment has a deleterious influence on the mechanical properties of coatings on Mg-6 wt pct Al alloy.