Therapies such as direct tension-free microsurgical repair or transplantation of a nerve autograft,are nowadays used to treat traumatic peripheral nerve injuries（PNI）,focused on the enhancement of the intrinsic rege...Therapies such as direct tension-free microsurgical repair or transplantation of a nerve autograft,are nowadays used to treat traumatic peripheral nerve injuries（PNI）,focused on the enhancement of the intrinsic regenerative potential of injured axons.However,these therapies fail to recreate the suitable cellular and molecular microenvironment of peripheral nerve repair and in some cases,the functional recovery of nerve injuries is incomplete.Thus,new biomedical engineering strategies based on tissue engineering approaches through molecular intervention and scaffolding offer promising outcomes on the field.In this sense,evidence is accumulating in both,preclinical and clinical settings,indicating that platelet-rich plasma products,and fibrin scaffold obtained from this technology,hold an important therapeutic potential as a neuroprotective,neurogenic and neuroinflammatory therapeutic modulator system,as well as enhancing the sensory and motor functional nerve muscle unit recovery.展开更多
Bone marrow mesenchymal stem cells were isolated from New Zealand white rabbits, culture-expanded and differentiated into Schwann cell-like cells. Autologous platelet-dch plasma and Schwann cell-like cells were mixed ...Bone marrow mesenchymal stem cells were isolated from New Zealand white rabbits, culture-expanded and differentiated into Schwann cell-like cells. Autologous platelet-dch plasma and Schwann cell-like cells were mixed in suspension at a density of 1 x 106 cells/mL, prior to introduction into a poly （lactic-co-glycolic acid） conduit. Fabricated tissue-engineered nerves were implanted into rabbits to bridge 10 mm sciatic nerve defects （platelet-rich plasma group）. Controls were established using fibrin as the seeding matrix for Schwann cell-like cells at identical density to construct tissue-engineered nerves （fibrin group）. Twelve weeks after implantation, toluidine blue staining and scanning electron microscopy were used to demonstrate an increase in the number of regenerating nerve fibers and thickness of the myelin sheath in the platelet-rich plasma group compared with the fibrin group. Fluoro-gold retrograde labeling revealed that the number of Fluoro-gold-positive neurons in the dorsal root ganglion and the spinal cord anterior horn was greater in the platelet-rich plasma group than in the fibrin group. Electrophysiological examination confirmed that compound muscle action potential and nerve conduction velocity were superior in the platelet-rich plasma group compared with the fibrin group. These results indicate that autologous platelet-rich plasma gel can effectively serve as a seeding matrix for Schwann cell-like cells to construct tissue-engineered nerves to promote perJpheral nerve regeneration.展开更多
背景:牙列缺损患者缺牙区的软硬组织不足是影响口腔种植治疗效果的重要因素,在拔牙后或种植体植入期间使用血小板浓缩物进行辅助治疗有望解决这一难题,改善口腔种植疗效。目的:本研究旨在通过检索近10年发表的有关血小板浓缩物在种植领...背景:牙列缺损患者缺牙区的软硬组织不足是影响口腔种植治疗效果的重要因素,在拔牙后或种植体植入期间使用血小板浓缩物进行辅助治疗有望解决这一难题,改善口腔种植疗效。目的:本研究旨在通过检索近10年发表的有关血小板浓缩物在种植领域应用的文献,总结目前文献研究中的详细情况及研究热点,进而探索该领域研究的流行趋势,为该领域未来研究及发展方向提供指引和参考。方法:在Web of Science数据库核心合集中检索在2012-2021年间发表的有关血小板浓缩物在口腔种植领域应用的文献,将检出文献进行去重及筛选后,使用Citespace 5.8 R3软件对筛选后的文献进行关键词与文献共被引等情况的分析,并将分析结果进行可视化展示。结果与结论:本研究共纳入有效文献328篇。2012-2021年间,血小板浓缩物在口腔种植领域应用的文献数量在前期呈略微上升,而在后期趋于平稳。在本研究纳入的文献中,通过对关键词的使用频次分析、关键词的聚类分析以及高被引文献的分析可知,在2012-2021年间,该领域的研究中血小板浓缩物与“自体骨”的临床疗效受到了学界的较多关注;同时,血小板浓缩物中富含的多种“生长因子”的相关文章也逐年增加。在口腔种植相关研究中,组织工程常用种子细胞“间充质干细胞”、临床种植骨量不足的常用材料“膜”及常用增骨术式“上颌窦提升术”均与血小板浓缩物存在一定的研究热度。展开更多
文摘Therapies such as direct tension-free microsurgical repair or transplantation of a nerve autograft,are nowadays used to treat traumatic peripheral nerve injuries（PNI）,focused on the enhancement of the intrinsic regenerative potential of injured axons.However,these therapies fail to recreate the suitable cellular and molecular microenvironment of peripheral nerve repair and in some cases,the functional recovery of nerve injuries is incomplete.Thus,new biomedical engineering strategies based on tissue engineering approaches through molecular intervention and scaffolding offer promising outcomes on the field.In this sense,evidence is accumulating in both,preclinical and clinical settings,indicating that platelet-rich plasma products,and fibrin scaffold obtained from this technology,hold an important therapeutic potential as a neuroprotective,neurogenic and neuroinflammatory therapeutic modulator system,as well as enhancing the sensory and motor functional nerve muscle unit recovery.
基金supported by the High Education Development Foundation of Shandong Province,No.J11LF22
文摘Bone marrow mesenchymal stem cells were isolated from New Zealand white rabbits, culture-expanded and differentiated into Schwann cell-like cells. Autologous platelet-dch plasma and Schwann cell-like cells were mixed in suspension at a density of 1 x 106 cells/mL, prior to introduction into a poly （lactic-co-glycolic acid） conduit. Fabricated tissue-engineered nerves were implanted into rabbits to bridge 10 mm sciatic nerve defects （platelet-rich plasma group）. Controls were established using fibrin as the seeding matrix for Schwann cell-like cells at identical density to construct tissue-engineered nerves （fibrin group）. Twelve weeks after implantation, toluidine blue staining and scanning electron microscopy were used to demonstrate an increase in the number of regenerating nerve fibers and thickness of the myelin sheath in the platelet-rich plasma group compared with the fibrin group. Fluoro-gold retrograde labeling revealed that the number of Fluoro-gold-positive neurons in the dorsal root ganglion and the spinal cord anterior horn was greater in the platelet-rich plasma group than in the fibrin group. Electrophysiological examination confirmed that compound muscle action potential and nerve conduction velocity were superior in the platelet-rich plasma group compared with the fibrin group. These results indicate that autologous platelet-rich plasma gel can effectively serve as a seeding matrix for Schwann cell-like cells to construct tissue-engineered nerves to promote perJpheral nerve regeneration.
文摘背景:牙列缺损患者缺牙区的软硬组织不足是影响口腔种植治疗效果的重要因素,在拔牙后或种植体植入期间使用血小板浓缩物进行辅助治疗有望解决这一难题,改善口腔种植疗效。目的:本研究旨在通过检索近10年发表的有关血小板浓缩物在种植领域应用的文献,总结目前文献研究中的详细情况及研究热点,进而探索该领域研究的流行趋势,为该领域未来研究及发展方向提供指引和参考。方法:在Web of Science数据库核心合集中检索在2012-2021年间发表的有关血小板浓缩物在口腔种植领域应用的文献,将检出文献进行去重及筛选后,使用Citespace 5.8 R3软件对筛选后的文献进行关键词与文献共被引等情况的分析,并将分析结果进行可视化展示。结果与结论:本研究共纳入有效文献328篇。2012-2021年间,血小板浓缩物在口腔种植领域应用的文献数量在前期呈略微上升,而在后期趋于平稳。在本研究纳入的文献中,通过对关键词的使用频次分析、关键词的聚类分析以及高被引文献的分析可知,在2012-2021年间,该领域的研究中血小板浓缩物与“自体骨”的临床疗效受到了学界的较多关注;同时,血小板浓缩物中富含的多种“生长因子”的相关文章也逐年增加。在口腔种植相关研究中,组织工程常用种子细胞“间充质干细胞”、临床种植骨量不足的常用材料“膜”及常用增骨术式“上颌窦提升术”均与血小板浓缩物存在一定的研究热度。
