The first factor affecting dryland winter wheat grain yield under various mulching measures: Spike number

Water is the key factor limiting dryland wheat grain yield.Mulching affects crop yield and yield components by affecting soil moisture.Further research is needed to determine the relationships between yield components and soil moisture with yield,and to identify the most important factor affecting grain yield under various mulching measures.A long-term 9-yearifeld experiment in the Loess Plateau of Northwest China was carried out with three treatments:no mulch (CK),plastic mulch (M_(P)) and straw mulch (M_(S)).Yield factors and soil moisture were measured,and the relationships between them were explored by correlation analysis,structural equation modeling and significance analysis.The results showed that compared with CK,the average grain yields of M_(P) and M_(S) increased by 13.0and 10.6%,respectively.The average annual grain yield of the M_(P) treatment was 134 kg ha^(–1) higher than the M_(S) treatment.There were no significant differences in yield components among the three treatments (P<0.05).Soil water storage of the M_(S) treatment was greater than the M_(P) treatment,although the differences were not statistically signifiant.Soil water storage during the summer fallow period (SWSSF) and soil water storage before sowing (SWSS) of M_(S) were significantly higher than in CK,which increased by 38.5 and 13.6%,respectively.The relationship between M_(P) and CK was not statistically significant for SWSSF,but the SWSS in M_(P) was significantly higher than in CK.In terms of soil water storage after harvest (SWSH) and water consumption in the growth period(ET),there were no signi?cant differences among the three treatments.Based on the three analysis methods,we found that spike number and ET were positively correlated with grain yield.However,the relative importance of spike number to yield was the greatest in the M_(P )and M_(S) treatments,while that of ET was the greatest in CK.Suifcient SWSSF could indirectly increase spike number and ET in the three treatments.Based on these results,mulch can improve yield and soil water storage.The most important factor affecting the grain yield of dryland wheat was spike number under mulching,and ET with CK.These findings may help us to understand the main factors influencing dryland wheat grain yield under mulching conditions compared to CK.

Can soil organic carbon sequestration and the carbon management index be improved by changing the film mulching methods in the semiarid region?

Plastic film mulching has been widely used to increase maize yield in the semiarid area of China.However, whether long-term plastic film mulching is conducive to agricultural sustainability in this region remains controversial.A field experiment was initiated in 2013 with five different film mulching methods:(i) control method, flat planting without mulching (CK),(ii) flat planting with half film mulching (P),(iii) film mulching on ridges and planting in narrow furrows(S),(iv) full film mulching on double ridges (D), and (v) film mulching on ridges and planting in wide furrows (R).The effects on soil organic carbon (SOC) content, storage, and fractions, and on the carbon management index (CMI)were evaluated after nine consecutive years of plastic film mulching.The results showed that long-term plastic film mulching generally maintained the initial SOC level.Compared with no mulching, plastic film mulching increased the average crop yield, biomass yield, and root biomass by 48.38, 35.06, and 37.32%, respectively, which led to the improvement of SOC sequestration.Specifically, plastic film mulching significantly improved CMI, and increased the SOC content by 13.59%, SOC storage by 7.47%and easily oxidizable organic carbon (EOC) by 13.78%on average,but it reduced the other labile fractions.SOC sequestration and CMI were improved by refining the plastic film mulching methods.The S treatment had the best effect among the four mulching methods, so it can be used as a reasonable film mulching method for sustainable agricultural development in the semiarid area.

Effects of film-stalk spaced dual mulching system on corn growth and yield

Film-stalk spaced dual mulching is a new type of cultivation measure that is increasingly highlighted in semi-arid areas in China.Despite its potential,there is limited understanding of how different mulching materials affect both soil quality and crop yield in these areas.To address this gap,we conducted a two-year(2020-2021)field experiment in central China to explore the yield-enhancing mechanisms and assess the impact of various mulching materials on soil and corn yield.The experiment comprised six treatments,i.e.,plastic film-whole stalk spaced mulching in fall(PSF),plastic film-whole stalk spaced mulching in spring(PSS),black and silver plastic film-whole stalk spaced mulching in spring(BPSS),biodegradable film-whole stalk spaced mulching in spring(BSS),liquid film-whole stalk spaced mulching in spring(LSS),and non-mulching cultivation(CK).Results revealed that BPSS demonstrated the most significant yield increase,surpassing CK by a notable 10.0%and other mulching treatments by 2.4%-5.9%.The efficacy of BPSS lied in its provision of favorable hydrothermal conditions for corn cultivation,particularly during hot season.Its cooling effect facilitated the establishment of optimal temperature conditions relative to transparent mulching,leading to higher root growth indices(e.g.,length and surface area),as well as higher leaf photosynthetic rate and dry matter accumulation per plant.Additionally,BPSS maintained higher average soil moisture content within 0-100 cm depth compared with biodegradable mulching and liquid mulching.As a result,BPSS increased activities of urease,catalase,and alkaline phosphatase,as well as the diversity and abundance of soil bacteria and fungi in the rhizosphere zone of corn,facilitating nutrient accessibility by the plant.These findings suggest that selecting appropriate mulching materials is crucial for optimizing corn production in drought-prone areas,highlighting the potential of BPSS cultivation.

Performance of Bt cotton evaluated in relation to mulching and weed control measures in northwest India

Background Weed infestation in cotton has been reported to offer severe competition and cause yield reduction to a large extent.Weeding via cultural practices is time consuming,tedious,and expensive due to long duration of cotton crop and regular monsoon rains during cotton production in India.Chemical weed control has been successfully utilized in cotton in the recent past.However,continuous use of similar herbicides leads to resistance in weeds against herbicides.And when sprayed to the field,herbicides not only suppress weeds but leave undesirable residues in the soil that are hazardous to the environment.Therefore,a study was performed at cotton research area at Chaudhary Charan Singh Haryana Agricultural University,Hisar,Haryana during two consecutive kharif seasons(2020 and 2021)to determine the most suitable and sustainable weed management strategy through the integration of chemical and cultural methods.Results Mulching with rice straw of 7.5 t ha^(-1)resulted in significantly higher cotton seed yield(3189 and 3084 kg ha^(-1))and better weed control in comparison to no mulch treatments(2990 and 2904 kg ha^(-1))in 2020 and 2021,respectively.Among various weed management levels,the significantly lowest cotton seed yield was recorded in untreated control(1841 and 1757 kg·ha^(-1)during 2020 and 2021,respectively)in comparison to other treatments while all other treatments were statistically at par with each other during both years of crop experimentation.Conclusion Mulching with rice straw of 7.5 t·ha^(-1)along with a pre-emergence application of pendimethalin(active ingredient)at 1.5 kg·ha^(-1)fb(followed by)one hoeings at 45 days after sowing(DAS)and fb glyphosate 2 kg·ha^(-1)(Shielded spray)at 90 DAS is a viable option for effective control of grassy and broadleaved weeds in Bt cotton in north-west India.

Rice Cultivation under Film Mulching Can Improve Soil Environment and Be Beneficial for Rice Production in China

Rice cultivation under film mulching is an integrated management technology that can conserve water, increase soil temperature, improve yield, and enhance water and nitrogen use efficiencies. Despite these advantages, the system does have its drawbacks, such as soil organic matter reduction and microplastic pollution, which impede the widespread adoption of film mulching cultivation in China. Nonetheless, the advent of degradable film, controlled-release fertilizer, organic fertilizer, and film mulching machinery is promoting the development of rice film mulching cultivation. This review outlines the impact of rice cultivation under film mulching on soil moisture, soil temperature, soil fertility, greenhouse gas emissions, weed control, and disease and pest management. It also elucidates the mechanism of changes in rice growth, yield and quality, water use efficiency, and nitrogen use efficiency. This paper incorporates a review of published research articles and discusses some uncertainties and shortcomings associated with rice cultivation under film mulching. Consequently, prospective research directions for the technology of rice film mulching cultivation are outlined, and recommendations for future research into rice cultivation under film mulching are proposed.

Effect of Different Mulching Materials on Growth Parameters and Yield of Okra (Abelmoschus esculentus) Production in Minna, Nigeria

During the 2022 growing season (August to November) at the Federal University of Technology, Minna School farm, Niger State, an experiment was conducted to investigate the influence of mulching on Okras growth, yield, and moisture content was examined across four distinct growth stages (initial, development, mid, and late) and at varying soil depths (0 - 30 cm and 30 - 60 cm). The study employed a randomised complete block design with four replications, encompassing control (T0), groundnut shells mulch (T1), black polythene mulch (T2), and white polythene mulch (T3) as treatments. The highest average Okra fresh pod yield, amounting to 23.4 t/ha, was achieved by implementing white plastic mulch, contrasting with the control treatment, which yielded the lowest at 22 t/ha. Notably, the control plots exhibited yield reductions of up to 32% compared to the plots employing white plastic mulching. The utilisation of mulch had a notable impact on the overall crop yield, with the superior quality evident in the treatment employing white plastic mulch (26 t/ha). The control treatment exhibited the lowest quality at 24.3 t/ha. Groundnut shell mulch influenced moisture conservation, but no significant variance was observed compared to the control plots. Therefore, the study suggests that polythene mulch may be the most suitable type to enhance the quality of okra production by conserving soil moisture. Among the biodegradable and non-biodegradable mulches used in this study, white polythene mulch was the most effective.

Effects of Film Mulching on the Growth of Silage Maize and Weed

[Objective] The aim was to provide scientific reference for the production of silage maize in correlated regions of Tibet.[Method] Effects of film mulching on the growth period,growth traits and yield of silage maize and weed were analyzed.[Result] Under the treatment of film mulching,the growth period of silage maize could be advanced for 7-16 d; the plant length,stem diameter and leaf area were increased; and the grain output was increased by 75.9%.According to the market price of silage maize in 2008,22 500 Yuan/hm^2 was increased.There were 13 kinds of weeds in silage maize field,which belonged to 13 genera,8 families.Among them,6 kinds of weeds belonged to Gramineae,among which Echinochloa crusgalli and Setaria viridis were dominated in the weed communities.Plantago depressa,Eragrostis pilosa and Malva verticillata var.chinensis could be totally controlled by the film mulching; meanwhile the others could be also controlled in a certain degree except Echinochloa crusgalli.[Conclusion] The plastic mulching planting is worth popularizing for the developing of animal husbandry and the incoming of the local farmers in Tibet.

Effects of Different Plastic films Mulching on Soil Temperature and Moisture, the Growth and Yield of Sugarcane

[Objective] The effects of different plastic films mulching on soil temperature and moisture, and growth and yield of sugarcane were discussed in order to provide references for using different plastic film in sugarcane pro-duction. [Method]Four kinds of plastic films viz., normal colorless transparent plastic film, milky photodegradation weeding plastic film, black plastic film and gray-black plastic film were used in sugarcane cultivation by using no film mulching as the control. Soil temperature and moisture were measured during plastic film mulching period, and sugarcane agronomic traits such as emergence rate, tillering rate, plant hight, stalk diameter and effective stalk number were investigated during growth period, the cane yield and economic benefits were calculated during harvest period. [Result] The results showed that plastic film mulching could significantly increase soil temperature and moisture. Com-pared with the control, soil temperature was increased by 0.3-0.8 ℃ in three plastic films mulching treatment except for gray-black plastic film mulching. The soil moisture of all mulching treatments was 10.1%-17.4% higher than the control. Furthermore, the seedling emergence rate, tillering rate, effective stalk number and cane yield also could be improved using plastic film mulching,which were increased by 0.8%-9.9%, 20.6%-34.9%, 5190-10980 stalks/hm^2and6.4%-14.9% as compared to the control,while plant height and stalk diameter were found to be no significant effect by plastic film mulching. The results of benefit analysis indicated that, milky photodegradation weeding film mulching had the highest economic benefit, the second were normal colorless transparent plastic film mulching and black plastic film mulching, which were 5 987.2, 1 876.5 and 1 813.5 Yuan/hm^2 higher than the control. The gray-black film mulching treatment had poor benefit.[Conclusion] The milky photodegradation weeding plastic film could be vigorously extended in sugarcane production. Normal colorless transparent plastic film and black plastic film could be ex-tended gradually as a new kind of plastic film. The grayblack film should not be used for its higher cost and more thickness.

Effects of Plastic Film Mulching on Physical Characters of Soil and Yield and Yield Components of Sweet Potato

Field experiments were carried out to study the effects of plastic film mulching on soil physical characters, including soil temperature, soil moisture content and soil bulk density, and yield and yield components of sweet potato. The results showed that plastic filming mulching increased soil temperature. Considering the soil temperature-increasing effect, the treatments ranked as black plastic film treatment 〉 white plastic film treatment 〉 control. However, with the deepening of soil layer, the warming effect of plastic film mulching was weakened. Black or white plastic film mulching was conducive to low T/R value, especially in the early growth stage of sweet potato. Plastic film mulching significantly improved the storage root yield of sweet potato. In terms of yield-improving effect, the treatments ranked as black plastic film treatment 〉 white plastic film treatment 〉 control. The storage root num- ber per plant showed a downward trend, but the weight of single storage root was increased.

Effects of Different Tillage and Mulching Modes on Tobacco Output Value and Soil Quality

Based on three years of long-term fixed-site field trial, the effects of differ- ent tillage and mulching modes on the yield and output value of tobacco and soil quality were investigated. The results showed that the yield and output value of to- bacco in tillage treatment were improved by 22.72 % and 37.23 % compared with non-tillage treatment, respectively; the yield and output value of tobacco in rotation treatment were improved by 1.83% and 19.41% compared with continuous cropping treatment, respectively; the yield and output value of tobacco in straw mulching treatment were improved by 3.55% and 2.4% compared with non-straw mulching treatment, respectively, which indicated that tillage, rotation and straw mulching could improve the yield and output value of tobacco to a certain extent; especially, the yield and output value of tobacco increased significantly after plowing under rotation conditions. The contents of available phosphorus （AP）, alkali-hydrolyzable nitrogen （AN）, organic matter （OM）, total nitrogen （TN） and total phosphorus （TP） in non- tillage treatment were 35.14%, 9.92%, 9.57%, 4.40% and 34.16% higher compared with tillage treatment; especially, under non-tillage conditions, soil pH and contents of available potassium （AK）, AP, AN,OM,TN,TP and total potassium （TK） in continu- ous cropping field were 2.01%, 48.68%, 73.09%, 11.45%, 7.71%, 7.31%, 47.68% and 11.78% higher compared with rotation field, indicating that non-tillage treatment and continuous cropping could improve the total content and available content of organic matter, nitrogen and phosphorus. Therefore, from the perspective of soil fer- tility improvement and sustainable tobacco production, continuous cropping under non-tillage conditions might be the most appropriate cropping pattern for local soil fertility improvement; aiming at improving the yield and output value of tobacco, green manure-tobacco→, green manure/wheat/maize→green manure-tobacco ro- tation might be the most appropriate cropping pattern.

Effects of Ground Mulching Treatments on Soil Moisture Content in Second-generation Seed Garden of Pinus elliottii

Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that： （1） there were extreme differences in soil moisture content Ⅰ and soil moisture content Ⅲ, but no obvious difference in soil moisture content II was observed; and （2） the high-to-low order of soil moisture content Ⅰ under different ground mulching treatments was white film〉sawdust〉black film〉the control, and the highest value was higher than the lowest one by 26.69%; the high-to-low order of soil moisture content Ⅱ under different ground mulching treatments was white film〉black film〉sawdust〉the control, and the highest value was higher than the lowest one by 20.64%; and the high-to-low order of soil moisture content Ⅲ under different ground mulching treatments was black film〉white film〉sawdust〉the control, and the highest value was higher than the lowest one by 26.61%.

Effects of Different Mulching Methods on Primary Substances and Secondary Substances of Lycium barbarum L.

[Objective] The aim was to provide certain theoretical basis for stuides on the high yield and high quality cultivation of Lycium barbarum L..[Method] Under yield conditions,the accumulation of primary substances and secondary substances of Lycium barbarum L.was studied under different mulching treatments.[Result] Different mulching methods all had a certain effects on the contents of main primary substances and main secondary substances in Lycium barbarum L.fruit.Among them,carbohydrates and flavonoids contents in Lycium barbarum L.were both obviously increased under straw-film mulching;carotenoid content was decreased relatively;hundred leaf weight,1 000-grain weight and yield of Lycium barbarum L.were increased to a certain extent,but there was no obvious effect on the shape of fruit.Besides,the yield of Lycium barbarum L.had no significant positive correlation with total carbohydrate and polysaccharide,almost had no correlation with flavonoids,and had no significant negative correlation with carotenoid.[Conclusion] The reasonable mulching could improve the yield and quality of Lycium barbarum L..

Light Simplified Millet Production Technique Adopting Film Mulching and Hole Sowing

In order to solve the problem that dry-land foxtail millet production completely relies on rainwater with low instable yield and tedious cultivation, Millet Research Institute of Hebei Academy of Agriculture and Forestry Sciences integrated a light simplified production technique integrating film mulching, hole sowing and fertilization with mechanized production, forming the light simplified foxtail millet production technique adopting film mulching and hole sowing. This study introduced the light simplified foxtail millet production technique adopting film mulching and hole sowing, including main links such as preparation before sowing, sowing, attached agricultural machines, field management, harvest and residual film recovery.

Effects of Water-permeability Plastic Film Mulching plus Bunch Planting on Root and Yield of Foxtail Millet

Effects of water-permeability plastic film plus bunch planting on root growth and development and yield of foxtail millet were studied by randomized block design. The results showed that water-permeability plastic film mulching plus bunch planting had a significant promoting effect on root growth and development and yield of foxtail millet. Compared with the CK, the total root length, total surface area, total root volume and number of. root tips increased by 51.30%, 47.89%, 48.39% and 41.63%, respectively. The yield increased by 48.57%, and there was significant positive correlation between root length, total surface area, total volume, number of root tips and dry matter weight of roots with yield. Developed roots are the main reason for the yield increasing effect of water-permeability plastic film mulching plus bunch planting.

Effect of Planting in Furrow and Whole Plasticfilm Mulching on Double Ridges on Improvement of Degraded Desert Grassland

[Objective] This study aimed to provide theoretical basis and technologies for the application of planting in furrow and whole plastic-film mulching on double ridges. [Method] The conventional method was used to re-seed the bunch Gramineous forage in the degraded desert grassland in Yanchi, Ningxia Hui Autonomous Region. Four kinds of conservation treatments, namely, ridging with black film mulching, ridging with transparent film mulching, ridging with no mulching and the flatplanting with no ridging and mulching were conducted to the forage to analyze the effects of each treatment on soil moisture and seedling growth. [Result] From July to November, the moisture contents of ridging with black film mulching, ridging with transparent film mulching, ridging with no mulching and the flatplanting were respectivery 9.88%, 9.24%, 8.75% and 8.13%, showing significant differences among the treatments; the survival rates of re-seeding forage were 96.2% , 93.4% , 45.6% and 28.3% , and the mulching treatment showed significant difference with the unmulching treatments in survival rate. The treatment with black film mulching showed great advantage that its moisture content on ridge top had the buffering effect of ＂load shifting＂, and the soil moisture content of black mulching treatment increased 21.5% than the flatplanting. Black film mulching also had the largest water supplement amount in soil below 1 m. Although mulching cost too much, its overall benefits were higher than that with no mulching. [Conclusion] This study lays the experimental basis for the application of planting in furrow and whole plastic-film mulching on double ridges in improving degraded grassland desertification.

Soil mulching can mitigate soil water defi ciency impacts on rainfed maize production in semiarid environments

Temporally irregular rainfall distribution and inefficient rainwater management create severe constraints on crop production in rainfed semiarid areas. Gravel and plastic film mulching are effective methods for improving agricultural productivity and water utilization. However, the effects of these mulching practices on soil water supply and plant water use associated with crop yield are not well understood. A 3-yr study was conducted to analyze the occurrence and distribution of dry spells in a semiarid region of Northwest China and to evaluate the effects of non-mulching （CK）, gravel mulching （GM） and plastic film mulching （FM） on the soil water supply, plant water use and maize （Zea mays L.） grain yield. Rainfall analysis showed that dry spells of ≥5 days occurred frequently in each of 3 yr, accounting for 59.9-69.2% of the maize growing periods. The 〉15-d dry spells during the jointing stage would expose maize plants to particularly severe water stress. Compared with the CK treatment, both the GM and FM treatments markedly increased soil water storage during the early growing season. In general, the total evapotranspiration （ET） was not significantly different among the three treatments, but the mulched treatments significantly increased the ratio of pre- to post-silking ET, which was closely associated with yield improvement. As a result, the grain yield significantly increased by 17.1, 70.3 and 16.7% for the GM treatment and by 28.3, 87.6 and 38.2% for the FM treatment in 2010, 2011 and 2012, respectively, compared with the CK treatment. It＇s concluded that both GM and FM are effective strategies for mitigating the impacts of water deficit and improving maize production in semiarid areas. However. FM is more effective than GM.

The status and distribution characteristics of residual mulching film in Xinjiang, China

Pollution of residual plastic film in arable lands is a severe problem in China. In this study, the status of residual film and influential factors were investigated using the methods of farm survey in combination with questionnaires and quadrat sampling at a large number of field sites in Xinjiang Uygur Autonomous Region, China. The results showed that the amount of film utilization increased largely and reached to 1.8×10~5 t in 2013. Similarly, the mulching area also substantially increased in recent decades, and reached to 2.7×10~5 ha in the same year. According to the current survey, 60.7% of the sites presented a greater mulch residue than the national film residue standard（75 kg ha^（–1））, and the maximum residual amount reached 502.2 kg ha^（–1） in Turpan, Xinjiang. The film thickness, the mulching time and the crop type all influenced mulch residue. The thickness of the film had significantly negative correlation with the amount of residual film（P0.05）, while the mulching years had significantly positive correlation with it（P0.05）. The total amount of residual film in Xinjiang was 3.43×105 t in 2011, which accounted for 15.3% of the cumulative dosage of mulching. Among all the crops, the cotton fields had the largest residual amount of mulch film（158.4 kg ha^（–1））, and also the largest contribution（2.6×10~5 tons） to the total amount of residual film in Xinjiang.

Alternate row mulching optimizes soil temperature and water conditions and improves wheat yield in dryland farming

Straw mulching allows for effective water storage in dryland wheat production. Finding a suitable straw mulching model that facilitates wheat growth was the objective of this study. A 2-year field experiment was conducted to investigate the effects of two straw mulching patterns （FM, full coverage within all the rows; HM, half coverage within alternate rows） and two mulching rates （4.5 and 9.0 t ha^-1） on soil moisture, soil temperature, grain yield, and water use efficiency （WUE） of winter wheat in northern China, with no mulching （M0） as the control. Results showed that mulching increased the soil water storage in all growth stages under high mulching rates, with a stronger effect in later growth stages. Water storage under the HM model was greater in later stages than under the FM model. Soil water content of HM groups was higher than that of FM groups, especially in surface soil layers. Evapotranspiration decreased in mulched groups and was higher under high mulching rates. Aboveground biomass during each growth stage under the HM model was higher than that under M0 and FM models with the same mulched rate, leading to a relatively higher grain yield under the HM model. Mulching increased WUE, a trend that was more obvious under HM9.0 treatment. Warming effect of soil temperature under the HM pattern persisted longer than under the FM model with the same mulching rates. Accumulated soil temperature under mulched treatments increased, and the period of negative soil temperature decreased by 9-12 days under FM and by 10-20 days under HM. Thus, the HM pattern with 9.0 t ha^-1 mulching rate is beneficial for both soil temperature and water content management and can contribute to high yields and high WUE for wheat production in China.

Mulching with plastic film improved the root quality of summersown sweet potato(Ipomoea batatas(L).Lam.)in northern China

The root quality of sweet potato cultivated during the summer season is poor in northern China;thus,this study was conducted to determine whether root quality could be improved through mulching with plastic film(MPF).The effect of MPF on root starch and its composition,the activity of starch synthesis enzymes,and other quality-related parameters were investigated in two purple flesh sweet potato cultivars,Jishu 18 and Ayamurasaki(Aya).The results indicated that root dry matter,anthocyanin content,adenosine triphosphate(ATP),and starch content were higher in both cultivars under the MPF treatment than those under the control treatment.The root adenosine diphosphate glucose pyrophosphorylase/uridine diphosphate glucose pyrophosphorylase(ADPGPPase/UDPGPPase)activity and adenosine triphosphatease(ATPase)activity were increased using MPF.However,under the MPF treatment,the amylose content,soluble sugar content,and granule-bound synthase(GBSS)activity increased in Jishu 18 but decreased in Aya,and the amylopectin content,protein content,and soluble starch synthase(SSS)activity decreased in Jishu 18 but increased in Aya.Therefore,MPF seems benifit to improve the quality of sweet potato,but the effects of this treatment condition may be dependent on the cultivar.

Buried straw layer and plastic mulching increase microflora diversity in salinized soil

Salt stress has been increasingly constraining crop productivity in arid lands of the world. In our recent study, salt stress was aleviated and crop productivity was improved remarkably by straw layer burial plus plastic iflm mulching in a saline soil. However, its impact on the microlfora diversity is not wel documented. Field micro-plot experiments were conducted from 2010 to 2011 using four tilage methods： （i） deep tilage with plastic iflm mulching （CK）, （i） straw layer burial at 40 cm （S）, （ii） straw layer burial plus surface soil mulching with straw material （S＋S）, and （iv） plastic iflm mulching plus buried straw layer （P＋S）. Culturable microbes and predominant bacterial communities were studied; based on 16S rDNA, bacterial com-munity structure and abundance were characterized using denaturing gradient gel electrophoresis （DGGE） and polymerase chain reaction （PCR）. Results showed that P＋S was the most favorable for culturable bacteria, actinomyces and fungi and induced the most diverse genera of bacteria compared to other tilage methods. Soil temperature had signiifcant positive correlations with the number of bacteria, actinomyces and fungi （P〈0.01）. However, soil water was poorly correlated with any of the microbes. Salt content had a signiifcant negative correlation with the number of microbers, especialy for bacteria and fungi （P〈0.01）. DGGE analysis showed that the P＋S exhibited the highest diversity of bacteria with 20 visible bands folowed by S＋S, S and CK. Moreover, P＋S had the highest similarity （68%） of bacterial communities with CK. The major bacterial genera in al soil samples wereFirmicutes,Proteobacteria andActinobacteria. Given the considerable increase in microbial growth, the combined use of straw layer burial and plastic iflm mulching could be a practical option for aleviating salt stress effects on soil microbial community and thereby improving crop production in arid saline soils.