The effects of Timentin and cefotaxime （Cef） on shoot regeneration of the London plane tree （Platanus acerifolia Willd.） and their use for the suppression ofAgrobacterium tumefaciens in Agrobacterium-mediated gene...The effects of Timentin and cefotaxime （Cef） on shoot regeneration of the London plane tree （Platanus acerifolia Willd.） and their use for the suppression ofAgrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L^-1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L^-1; however, 300 mg·L^-1 Timentin was shown to facilitate shoot regeneration significantly and the regeneration frequency increased from 64% （control） to 88%. Effective suppression of A. tumefaciens could be obtained with 500 mg·L^-1 Cef, but plant regeneration was completely inhibited at this level. The A. tumefaciens on infected P. acerifolia leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L^-1 Timentin. Considering the effect of Cef and Timentin on plant regeneration and suppression of Agrobacteria, Timentin at 300 mg·L^-1 is the preferred application in .4. tumefaciens-mediated transformation ofP acerifolia.展开更多
The title compound,3β-acetoxy-20（29）-lupen-28-aldehyde,is a pentacyclic triterpene which was first isolated from the acetyl acetate fraction of Platanus acerifolia Willd bark and characterized by 1 H-NMR and 13 C-N...The title compound,3β-acetoxy-20（29）-lupen-28-aldehyde,is a pentacyclic triterpene which was first isolated from the acetyl acetate fraction of Platanus acerifolia Willd bark and characterized by 1 H-NMR and 13 C-NMR.Besides,the crystal of the compound was acquired,and the relative configuration of 3β-acetoxy-20（29）-lupen-28-aldehyde was determined by single-crystal X-ray diffraction analysis for the first time.It crystallizes in the monoclinic system,space group C2 with a=14.428（3）,b=6.6620（13）,c=30.399（6）,β=103.73（3）°,Z=4,C32 H50 O3,M r=482.72,Dc =1.130 g/cm 3,V=2838.5（10）3,μ（MoKa）=0.070 mm-1,F（000）= 1064,the final R=0.0657 and wR=0.1855 for 2839 independent reflections （R int=0.0802）and 1963 observed ones （I〉2（I））.Intramolecular van der waals force contributes to the stability of the structure.展开更多
基金This research is partially supported by the National Natural Science Foundation of China （Grant No. 30371015） the Bureau of Science and Technology of Wuhan. The kindness of doctor Lin Yong-jun in providing Agrobacterium tumefaciens strains EHA 105, harboring the binary vector pCAMBI2301 for this experiment is greatly appreciated. We thank all the colleagues in our laboratory, especially doctor Gao Li-ping, for constructive discussion and technical support.
文摘The effects of Timentin and cefotaxime （Cef） on shoot regeneration of the London plane tree （Platanus acerifolia Willd.） and their use for the suppression ofAgrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L^-1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L^-1; however, 300 mg·L^-1 Timentin was shown to facilitate shoot regeneration significantly and the regeneration frequency increased from 64% （control） to 88%. Effective suppression of A. tumefaciens could be obtained with 500 mg·L^-1 Cef, but plant regeneration was completely inhibited at this level. The A. tumefaciens on infected P. acerifolia leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L^-1 Timentin. Considering the effect of Cef and Timentin on plant regeneration and suppression of Agrobacteria, Timentin at 300 mg·L^-1 is the preferred application in .4. tumefaciens-mediated transformation ofP acerifolia.
基金supported by Independent Research Program (cultivation for National Natural Science Foundation of China) of Nanjing University of Science and Technology (No. 2010GJPY009)
文摘The title compound,3β-acetoxy-20（29）-lupen-28-aldehyde,is a pentacyclic triterpene which was first isolated from the acetyl acetate fraction of Platanus acerifolia Willd bark and characterized by 1 H-NMR and 13 C-NMR.Besides,the crystal of the compound was acquired,and the relative configuration of 3β-acetoxy-20（29）-lupen-28-aldehyde was determined by single-crystal X-ray diffraction analysis for the first time.It crystallizes in the monoclinic system,space group C2 with a=14.428（3）,b=6.6620（13）,c=30.399（6）,β=103.73（3）°,Z=4,C32 H50 O3,M r=482.72,Dc =1.130 g/cm 3,V=2838.5（10）3,μ（MoKa）=0.070 mm-1,F（000）= 1064,the final R=0.0657 and wR=0.1855 for 2839 independent reflections （R int=0.0802）and 1963 observed ones （I〉2（I））.Intramolecular van der waals force contributes to the stability of the structure.
