AIM:To investigate the expressions of type I collagen, α2 integrin and β1 integrin in the posterior sclera of guinea pigs with defocus myopia and whether basic fibroblast growth factor (bFGF) injection inhibits the ...AIM:To investigate the expressions of type I collagen, α2 integrin and β1 integrin in the posterior sclera of guinea pigs with defocus myopia and whether basic fibroblast growth factor (bFGF) injection inhibits the formation and development of myopia by upregulating the expression of type I collagen, α2 integrin and β1 integrin. METHODS:After 14 days of treatment, the refractive state and axial length were measured and the levels of type I collagen, α2 integrin and β1 integrin were assayed in the posterior sclerae of groups of guinea pigs that wore a monocular-7D polymethylmethacrylate (PMMA) lens or had -7D lens wear followed by the peribulbar injection of Phosphate Buffer Solution (PBS) or bFGF. The untreated fellow eye served as a control. Guinea pigs with no treatment served as normal group. ·RESULTS:The results showed that 14 days of monocular defocus increased axial eye length and refraction, while bFGF delivery inhibited them markedly. Further, it was also found that the monocular-7D lens could decrease the levels of type I collagen, α2 integrin and β1 integrin expressions, while, unlike PBS, bFGF increased them significantly in comparison to contralateral control eyes and normal eyes. CONCLUSION:bFGF can prevent the formation anddevelopment of defocus myopia by upregulating the expressions of type I collagen, α2 integrin and β1 integrin. Taken together, our results demonstrate that bFGF promotes sclera remodeling to prevent myopia in guinea pigs.展开更多
Bismuth titanate (Bi4Ti3O12) platelets were prepared by molten salt method in a new salt system of CaCl2·NaCl at 650-750℃, using bismuth nitrate pentahydrate (Bi (NO3)3·H2O) and titanium butoxide (Ti...Bismuth titanate (Bi4Ti3O12) platelets were prepared by molten salt method in a new salt system of CaCl2·NaCl at 650-750℃, using bismuth nitrate pentahydrate (Bi (NO3)3·H2O) and titanium butoxide (Ti (OC4H9)4) as raw materials. The synthesis temperature of Bi4Ti3O12 platelets was decreased to 650℃ from 900-1100℃. The phase compositions and crystalline morphology of Bi4Ti3O12 platelets were investigated by XRD and SEM. The experimental results indicate that Bi4Ti3O12 platelets containing tetragonal and orthorhombic phase with the size of 1-3μm can be synthesized at 650℃ for 2 h, and the orthorhombic phase becomes the dominant phase at 750℃ for 5 h. The size and proportion of Bi4Ti3O12 platelets increase with the increment of the calcining temperature and holding time. The proportion of platelets increases to about ninety percent, and the platelets grow up to about 3-10μm at 750℃ for 5 h from 1-2μm at 650℃ for 2 h. This technical route provides a new low-temperature molten salt system for preparing platelets by molten salt methods.展开更多
文摘AIM:To investigate the expressions of type I collagen, α2 integrin and β1 integrin in the posterior sclera of guinea pigs with defocus myopia and whether basic fibroblast growth factor (bFGF) injection inhibits the formation and development of myopia by upregulating the expression of type I collagen, α2 integrin and β1 integrin. METHODS:After 14 days of treatment, the refractive state and axial length were measured and the levels of type I collagen, α2 integrin and β1 integrin were assayed in the posterior sclerae of groups of guinea pigs that wore a monocular-7D polymethylmethacrylate (PMMA) lens or had -7D lens wear followed by the peribulbar injection of Phosphate Buffer Solution (PBS) or bFGF. The untreated fellow eye served as a control. Guinea pigs with no treatment served as normal group. ·RESULTS:The results showed that 14 days of monocular defocus increased axial eye length and refraction, while bFGF delivery inhibited them markedly. Further, it was also found that the monocular-7D lens could decrease the levels of type I collagen, α2 integrin and β1 integrin expressions, while, unlike PBS, bFGF increased them significantly in comparison to contralateral control eyes and normal eyes. CONCLUSION:bFGF can prevent the formation anddevelopment of defocus myopia by upregulating the expressions of type I collagen, α2 integrin and β1 integrin. Taken together, our results demonstrate that bFGF promotes sclera remodeling to prevent myopia in guinea pigs.
文摘Bismuth titanate (Bi4Ti3O12) platelets were prepared by molten salt method in a new salt system of CaCl2·NaCl at 650-750℃, using bismuth nitrate pentahydrate (Bi (NO3)3·H2O) and titanium butoxide (Ti (OC4H9)4) as raw materials. The synthesis temperature of Bi4Ti3O12 platelets was decreased to 650℃ from 900-1100℃. The phase compositions and crystalline morphology of Bi4Ti3O12 platelets were investigated by XRD and SEM. The experimental results indicate that Bi4Ti3O12 platelets containing tetragonal and orthorhombic phase with the size of 1-3μm can be synthesized at 650℃ for 2 h, and the orthorhombic phase becomes the dominant phase at 750℃ for 5 h. The size and proportion of Bi4Ti3O12 platelets increase with the increment of the calcining temperature and holding time. The proportion of platelets increases to about ninety percent, and the platelets grow up to about 3-10μm at 750℃ for 5 h from 1-2μm at 650℃ for 2 h. This technical route provides a new low-temperature molten salt system for preparing platelets by molten salt methods.