Thrombosis formation on disrupted atherosclerotic plaque is the most common acuse of cardiovascular diseases, in the pathophysiology, increased platelet reactivity is a descriptor of the risk of cardiovascular events ...Thrombosis formation on disrupted atherosclerotic plaque is the most common acuse of cardiovascular diseases, in the pathophysiology, increased platelet reactivity is a descriptor of the risk of cardiovascular events in healthy persons and in patients with overt coronary artery disease. Regardless of the stimulus for activation platelet-platelet interation and thrombus formation is ultimately regulated through the GP Ⅱ b/Ⅲ a receptor展开更多
Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ ...Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature展开更多
目的探讨冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的变化。方法采用流式细胞仪三色免疫荧光分析检测健康老年人和冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的阳性百分率、蛋白酪氨酸磷酸酶(CD148)的几何荧光强度、P-选择素(CD6...目的探讨冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的变化。方法采用流式细胞仪三色免疫荧光分析检测健康老年人和冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的阳性百分率、蛋白酪氨酸磷酸酶(CD148)的几何荧光强度、P-选择素(CD62P)的阳性百分率、P-选择素(CD62P)的几何荧光强度及膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的阳性百分率、膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的几何荧光强度的表达水平。结果冠心病患者和健康老年人的血小板膜CD62P、CD62P几何荧光强度表达量相比较(6.24 vs 0.89,P<0.01;45.64 vs 25.38,P<0.05),差异有统计学意义;CD148、CD148几何荧光强度表达量相比较(7.12 vs 0.45,P<0.01;41.35 vs 20.12,P<0.01),差异有统计学意义;PAC-1及PAC-1几何荧光强度的表达量(22.64 vs 1.84,P<0.01;43.46 vs 22.45,P<0.01),差异有统计学意义,但与当日血压、血糖及血脂无相关关系。结论 CD62P及PAC-1、CD148是血小板活化的敏感指标,冠心病患者有血小板活化现象,为冠心病患者提供了理论检测指标。展开更多
文摘Thrombosis formation on disrupted atherosclerotic plaque is the most common acuse of cardiovascular diseases, in the pathophysiology, increased platelet reactivity is a descriptor of the risk of cardiovascular events in healthy persons and in patients with overt coronary artery disease. Regardless of the stimulus for activation platelet-platelet interation and thrombus formation is ultimately regulated through the GP Ⅱ b/Ⅲ a receptor
文摘Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature
文摘目的探讨冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的变化。方法采用流式细胞仪三色免疫荧光分析检测健康老年人和冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的阳性百分率、蛋白酪氨酸磷酸酶(CD148)的几何荧光强度、P-选择素(CD62P)的阳性百分率、P-选择素(CD62P)的几何荧光强度及膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的阳性百分率、膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的几何荧光强度的表达水平。结果冠心病患者和健康老年人的血小板膜CD62P、CD62P几何荧光强度表达量相比较(6.24 vs 0.89,P<0.01;45.64 vs 25.38,P<0.05),差异有统计学意义;CD148、CD148几何荧光强度表达量相比较(7.12 vs 0.45,P<0.01;41.35 vs 20.12,P<0.01),差异有统计学意义;PAC-1及PAC-1几何荧光强度的表达量(22.64 vs 1.84,P<0.01;43.46 vs 22.45,P<0.01),差异有统计学意义,但与当日血压、血糖及血脂无相关关系。结论 CD62P及PAC-1、CD148是血小板活化的敏感指标,冠心病患者有血小板活化现象,为冠心病患者提供了理论检测指标。
