To explore the effect of fertilizers on the yield and quality of Platostoma palustre,in this study,P.palustre was utilized as the research material,and field experiments were conducted with different application rates...To explore the effect of fertilizers on the yield and quality of Platostoma palustre,in this study,P.palustre was utilized as the research material,and field experiments were conducted with different application rates of compound fertilizer and organic fertilizer and non-targeted metabolomics analysis was further employed to compare and analyze the differences in the metabolic components between the compound fertilizer and organic fertilizer treatments.The results of field experiments demonstrated that both compound and organic fertilizers could promote the fresh weight,shade dry weight,and dry weight of P.palustre,with 450 kg hm−2 compound fertilizer and 4500 kg hm−2 organic fertilizer presenting the optimum effects.Non-targeted metabolomics revealed that 1096 metabolites were identified in 450 kg hm−2 compound fertilizer and 4500 kg hm−2 organic fertilizer,and 885 metabolites were annotated in the Human Metabolome Database(HMDB).There were 318 differential metabolites(DMs)found between the two treatments,and 263 metabolites were annotated in HMDB.The abundance of 2 phenolic compounds and 12 organic oxygen compounds in the treatment of 4500 kg hm−2 organic fertilizer was significantly higher than that of the 450 kg hm−2 compound fertilizer,while the abundance of 21 organic oxygen compounds,14 flavonoids,3 phenolic compounds,and 5 cinnamic acids and their derivatives was significantly up-regulated in 450 kg hm−2 compound fertilizer treatment.In addition,5 metabolic pathways were significantly enriched,and the flavone and flavonol biosynthesis was the most significantly differential metabolic pathway.These results suggested that the application of both compound fertilizers and organic fertilizers can increase the yield of P.palustre,but their effects on metabolites were different.This study has considerable implications for the planting and cultivation of P.palustre,furnishing a scientific foundation for an efficient and rational application of fertilizer.展开更多
基金funded by the Fund Projects of the Central Government in Guidance of Local Science and Technology Development(GuiKeZY22096020)Guangxi Key R&D Plan Project(2023AB23078)+1 种基金National Natural Science Foundation of China(82260750)Appropriate Technology Development and Promotion Project of Guangxi Traditional Chinese Medicine Administration(GZSY23-07).
文摘To explore the effect of fertilizers on the yield and quality of Platostoma palustre,in this study,P.palustre was utilized as the research material,and field experiments were conducted with different application rates of compound fertilizer and organic fertilizer and non-targeted metabolomics analysis was further employed to compare and analyze the differences in the metabolic components between the compound fertilizer and organic fertilizer treatments.The results of field experiments demonstrated that both compound and organic fertilizers could promote the fresh weight,shade dry weight,and dry weight of P.palustre,with 450 kg hm−2 compound fertilizer and 4500 kg hm−2 organic fertilizer presenting the optimum effects.Non-targeted metabolomics revealed that 1096 metabolites were identified in 450 kg hm−2 compound fertilizer and 4500 kg hm−2 organic fertilizer,and 885 metabolites were annotated in the Human Metabolome Database(HMDB).There were 318 differential metabolites(DMs)found between the two treatments,and 263 metabolites were annotated in HMDB.The abundance of 2 phenolic compounds and 12 organic oxygen compounds in the treatment of 4500 kg hm−2 organic fertilizer was significantly higher than that of the 450 kg hm−2 compound fertilizer,while the abundance of 21 organic oxygen compounds,14 flavonoids,3 phenolic compounds,and 5 cinnamic acids and their derivatives was significantly up-regulated in 450 kg hm−2 compound fertilizer treatment.In addition,5 metabolic pathways were significantly enriched,and the flavone and flavonol biosynthesis was the most significantly differential metabolic pathway.These results suggested that the application of both compound fertilizers and organic fertilizers can increase the yield of P.palustre,but their effects on metabolites were different.This study has considerable implications for the planting and cultivation of P.palustre,furnishing a scientific foundation for an efficient and rational application of fertilizer.
