Objective:To observe the effect of respiratory-function changes on urinary output and expression of aquaporin (AQP) in kidney tissue in bronchial asthma (BA) model mice.To explore the correlation between the lung cont...Objective:To observe the effect of respiratory-function changes on urinary output and expression of aquaporin (AQP) in kidney tissue in bronchial asthma (BA) model mice.To explore the correlation between the lung controlling breathing and the lung regulating the waterways,and observe regulation by the lung-diffusing herb platycodon root (Platycodon grandiflorus (JACQ.) A.DC.).Methods:Forty-five healthy female Balb/c mice were divided randomly into normal,model and platycodon root groups.The BA model was replicated by complex sensitization and stimulation with ovalbumin (OVA).Changes in airway resistance were detected using an AniRes2005 system,and 24-hour urine output collected by metabolic cages.Histopathologic changes in the lung and kidney were observed by H&rE staining.Expression of the mRNA of AQP1 and AQP2 was detected by reverse transcription-polymerase chain reaction,immunohistochemistry,and immunofluorescence.Results:Compared with the normal group,airway resistance in the inspiratory phase intensified in the model group (P <.01).Following the pathologic changes in lung tissue,but no significant change in kidney tissue,24-hour urinary output decreased significantly (P <.05),and levels of AQP1,AQP2 and their mRNA increased significantly in the model group (P <.01).Compared with the model group,airway resistance in the inspiratory phase was weakened(P <.01).The urinary output increased (P <.05),pathologic changes in lung tissues decreased,and renal expression of AQP1,AQP2 and their mRNA decreased significantly (P <.01) in the platycodon root group.Conclusion:Changes in respiratory function in BA model mice can affect how the lung regulates water pathways.Platycodon root diffusing the lung can ameliorate the respiratoryfunction and pathologic changes in the lung tissues of BA model mice,but also regulate urinary output and renal expression of AQP1 and AQP2.展开更多
The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-...The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-generic hybridization in 2009 and 2010, 2 repeat, and 88 normal plants were obtained. The characteristics of leaves, stems and branches were the same as female for F1 generation, F2 generation, backcross generation and S1 generation, and there were some different characteristics with female which were flower colors, branching habit and plant height. A lodging-resistance plant was selected in F1, which was about 75 cm high, deep pink flower color and developed lateral branches. The DNA groups of each generation were separated by 1% agarose gel electrophoresis and there were not significant differences. Among the each generation many flower colors and forms were obtained.展开更多
In order to promote the green,healthy and sustainable development of Platycodon grandiflorus industry,the current situations of P.grandiflorus industry development in Shandong Province were analyzed.That is,an advanta...In order to promote the green,healthy and sustainable development of Platycodon grandiflorus industry,the current situations of P.grandiflorus industry development in Shandong Province were analyzed.That is,an advantageous area has been gradually formed;product processing is diversified;industrialization development pattern is gradually emerging.Three problems in the development of P.grandiflorus industry were deeply analyzed.That is,it is difficult to control product quality;the problem of soil obstacles to continuous cropping is serious;the problem of talent shortage is prominent.Four countermeasures for the next development were put forward as follows:increasing government support,strengthening the construction of talent team,implementing crop rotation or intercropping,and increasing technology promotion.展开更多
A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus indu...A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus induction; MS medium containing 6\|BA 0.3 mg/L and IAA 0.1 mg/L was suitable for crowd bud propagation; 1/2 MS medium containing IAA 0.5 mg/L and ABT 0.1 mg/L is effective in inducing root with the ratio of 95%, fast propagation of P. grandiflorum by tissue culture method could be established, which will lay down the foundation of the breeding and clone of excellent varieties of P. grandiflorum.展开更多
基金This study was supported by the National Natural Science Foundation of China(81373503).
文摘Objective:To observe the effect of respiratory-function changes on urinary output and expression of aquaporin (AQP) in kidney tissue in bronchial asthma (BA) model mice.To explore the correlation between the lung controlling breathing and the lung regulating the waterways,and observe regulation by the lung-diffusing herb platycodon root (Platycodon grandiflorus (JACQ.) A.DC.).Methods:Forty-five healthy female Balb/c mice were divided randomly into normal,model and platycodon root groups.The BA model was replicated by complex sensitization and stimulation with ovalbumin (OVA).Changes in airway resistance were detected using an AniRes2005 system,and 24-hour urine output collected by metabolic cages.Histopathologic changes in the lung and kidney were observed by H&rE staining.Expression of the mRNA of AQP1 and AQP2 was detected by reverse transcription-polymerase chain reaction,immunohistochemistry,and immunofluorescence.Results:Compared with the normal group,airway resistance in the inspiratory phase intensified in the model group (P <.01).Following the pathologic changes in lung tissue,but no significant change in kidney tissue,24-hour urinary output decreased significantly (P <.05),and levels of AQP1,AQP2 and their mRNA increased significantly in the model group (P <.01).Compared with the model group,airway resistance in the inspiratory phase was weakened(P <.01).The urinary output increased (P <.05),pathologic changes in lung tissues decreased,and renal expression of AQP1,AQP2 and their mRNA decreased significantly (P <.01) in the platycodon root group.Conclusion:Changes in respiratory function in BA model mice can affect how the lung regulates water pathways.Platycodon root diffusing the lung can ameliorate the respiratoryfunction and pathologic changes in the lung tissues of BA model mice,but also regulate urinary output and renal expression of AQP1 and AQP2.
文摘The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-generic hybridization in 2009 and 2010, 2 repeat, and 88 normal plants were obtained. The characteristics of leaves, stems and branches were the same as female for F1 generation, F2 generation, backcross generation and S1 generation, and there were some different characteristics with female which were flower colors, branching habit and plant height. A lodging-resistance plant was selected in F1, which was about 75 cm high, deep pink flower color and developed lateral branches. The DNA groups of each generation were separated by 1% agarose gel electrophoresis and there were not significant differences. Among the each generation many flower colors and forms were obtained.
文摘In order to promote the green,healthy and sustainable development of Platycodon grandiflorus industry,the current situations of P.grandiflorus industry development in Shandong Province were analyzed.That is,an advantageous area has been gradually formed;product processing is diversified;industrialization development pattern is gradually emerging.Three problems in the development of P.grandiflorus industry were deeply analyzed.That is,it is difficult to control product quality;the problem of soil obstacles to continuous cropping is serious;the problem of talent shortage is prominent.Four countermeasures for the next development were put forward as follows:increasing government support,strengthening the construction of talent team,implementing crop rotation or intercropping,and increasing technology promotion.
文摘A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus induction; MS medium containing 6\|BA 0.3 mg/L and IAA 0.1 mg/L was suitable for crowd bud propagation; 1/2 MS medium containing IAA 0.5 mg/L and ABT 0.1 mg/L is effective in inducing root with the ratio of 95%, fast propagation of P. grandiflorum by tissue culture method could be established, which will lay down the foundation of the breeding and clone of excellent varieties of P. grandiflorum.
