In the present paper, the unicellular green alga Platymonas subcordiformis was entrapped in 2% (W/V) alginate, treated with CaCl2 at different concentrations (0. 5, 0.75, 1. 0, 1. 5 and 2. 0 mol/dm3), cultured at 15℃...In the present paper, the unicellular green alga Platymonas subcordiformis was entrapped in 2% (W/V) alginate, treated with CaCl2 at different concentrations (0. 5, 0.75, 1. 0, 1. 5 and 2. 0 mol/dm3), cultured at 15℃ and 25℃. The growth rate and morphology were studied and photosynthesis and respiration rates were measured. The optimum conditions of immobilized culture is in 0. 5 mol/dm3 of CaCl2, at 25℃. Photosynthesis rate of immobilized cells is higher than free cultivated cells, and that at 15℃ than at 25℃. Under scanning electronic microscope, it was shown that the immobilized cells had larger size and rougher surface. The relationship between growth of immobilized cells and abiotic factors and the prospects of applying immobilized cultivation are also discussed.展开更多
文摘In the present paper, the unicellular green alga Platymonas subcordiformis was entrapped in 2% (W/V) alginate, treated with CaCl2 at different concentrations (0. 5, 0.75, 1. 0, 1. 5 and 2. 0 mol/dm3), cultured at 15℃ and 25℃. The growth rate and morphology were studied and photosynthesis and respiration rates were measured. The optimum conditions of immobilized culture is in 0. 5 mol/dm3 of CaCl2, at 25℃. Photosynthesis rate of immobilized cells is higher than free cultivated cells, and that at 15℃ than at 25℃. Under scanning electronic microscope, it was shown that the immobilized cells had larger size and rougher surface. The relationship between growth of immobilized cells and abiotic factors and the prospects of applying immobilized cultivation are also discussed.
