Plectranthus hilliardiae hybrid的组织培养与快速繁殖(简报)

以Plectranthushilliardiaehybrid植株上带顶芽或带节的嫩茎段为外植体,在1/2MS+LH20mg/L+CAH20mg/L+YE20mg/L+4PU0.25mg/L培养基上,芽诱导效果较好,芽增殖倍数为3.2;在MS+IBA0.25mg/L生根培养基上,生根率达90%。

Plectranthus madagascanensis的组织培养及快速繁殖(简报)

以Plectranthus madagascanensis茎段为外植体进行组织培养,建立再生体系。结果表明,在1/2MS+6-BA0.1mg/L培养基上,芽诱导效果较好,增殖倍数为4.4;1/2MS+IBA0.5mg/L培养基适宜诱导不定根,诱导率达65%。

Phytochemical profiling and biological activity of Plectranthus amboinicus(Lour.) mediated by various solvent extracts against Aedes aegypti larvae and toxicity evaluation

Objective:To analyze the phytochemical compounds and to investigate the bio-toxic efficacy of various solvent extracts of Plectranthus amboinicus against mosquito larvae activity and lethality on non-targeting organisms.Methods:The methanol,ethyl acetate,hexane,and aqueous extracts of Plectranthus amboinicus were subjected to analyze the mosquitocidal activity against the dengue vector,Aedes aegypti and toxicity assays on zebra fish and brine shrimp.Three replications were performed,and negative control was also maintained.Amongst,ethyl acetate extract of Plectranthus amboinicus was chosen for the determination of bio-active compounds.Results:The mosquitocidal assays of methanol and ethyl acetate extracts of Plectranthus amboinicus showed the maximal activity with minimal concentration against the 4 th instar mosquito-larvae of Aedes aegypti through the following lethal concentration(LC50 and LC90)values:53.36&92.51μg/m L and 13.64&86.09μg/mL,respectively.In addition,the plant extracts showed no toxicity on zebra fish embryo and brine shrimp assays.The gas-chromatography analysis of the ethyl acetate extract of Plectranthus amboinicus revealed the presence of seven different compounds.Among them,PAEA-fraction 60 contained a major active bioactive compound,hexadecanoic acid,methyl ester(270.0).Conclusions:Plectranthus amboinicus possesses mosquitocidal properties and could be used as a potential alternative source for preparing the mosquitocidal agents.

Green Synthesis of Silver Nanoparticles Using Plectranthus Amboinicus Leaf Extract for Preparation of CMC/PVA Nanocomposite Film

In the present study,the biogenic silver nanoparticles have been synthesized using aqueous leaf extract of Plectranthus amboinicus(PA),which acted as both reducing and stabilizing agents.The PA synthesized silver nanoparticles were blended with carboxymethyl cellulose/polyvinyl alcohol(CMC/PVA)biocomposite.The prepared AgNPs as well as the biogenic AgNPs incorporated CMC/PVA films were investigated using UV-visible spectrophotometry,Fourier-transform infrared spectroscopy(FT-IR),dynamic light scattering(DLS),scanning electron microscope(SEM),and X–ray diffraction(XRD).The DLS results showed that biogenic AgNPs had the average particle size of 65.70 nm with polydispersity index of 0.44.The surface plasmon resonance of AgNPs,which was determined by UV-vis spectrophotometry,showed the value of 410.00 nm.These results therefore confirmed the reduction Ag+into Ag°and the formation of AgNPs in the medium.The SEM imaging showed that AgNPs was quasi-spherical and monodisperse.The XRD peaks at 33.07°,44.19°,64.58°and 77.47°confirmed the crystalline nature and presence of AgNPs.The CMC/PVA films that incorporated with AgNPs displayed best mechanical strength and morphological properties than the pure CMC/PVA film.The film of CMC/PVA-AgNPs exhibited significant antibacterial activities against Bacillus spizizenii,Staphylococcus aureus,Salmonella typhi and Escherichia Coli.

Antioxidant and Antibacterial Activities in the Leaf Extracts of Indian Borage (<i>Plectranthus amboinicus</i>)

The different solvent extracts of the leaves of Plectranthus amboinicus were evaluated for their antioxidant and anti-bacterial properties. The ethyl acetate extract of the leaves which had the highest polyphenolic content showed an appreciable DPPH radical scavenging activity as well as total antioxidant capacity as compared with extracts obtained using hexane, acetone, methanol, hydroalcohol and freeze dried form. The acetone extract of the leaves which had the second highest polyphenolic content showed appreciable antibacterial activity with least MIC values against the tested foodborne pathogens namely Staphylococcus aureus, Bacillus cereus, Escherichia coli and Yersinia enterocolitica. The findings indicated the potentiality of ethyl acetate and acetone extract of the leaves of Indian borage for use as biopreservatives as they showed high antioxidant and antibacterial activities.

<i>In Vitro</i>Micropropagation of a Valuable Medicinal Plant, <i>Plectranthus amboinicus</i>

The effect of the plant growth regulators benzyl amino purine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin (KIN) on in vitro shoot induction and proliferation of Plectranthus amboinicus was examined. Explants obtained from lateral shoots and apical shoots of P. amboinicus were inoculated on Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP, NAA and KIN. When the effect of each growth regulator was considered singly, the highest rate of shoot induction (80% of explants producing shoots) and highest number of shoots produced (2.4 shoots per explant) were obtained from lateral shoot explants cultured on MS media supplemented with 3.0 mg/L BAP within 6 - 7 weeks. Better results were obtained using MS medium supplemented with 1 mg/L BAP + 5 mg/L NAA. Shoot proliferation rose to 85%, while 5.7 shoots per explants were recorded. Among the different media tested for rooting, MS medium supplemented with 1.0 mg/L IBA was the most effective for root induction. The quality of the roots obtained was better than that obtained using MS media supplemented with NAA or IAA.

Phytochemical Screening, Toxicity, Analgesic and Anti-Pyretic Studies of Aqueous Leaf Extract of <i>Plectranthus barbatus</i>[Andrews. Engl.] in Rats

Plectranthus barbatus is a popular tropical perennial plant with a wide variety of traditional medicinal uses in tropical Africa, Hindu, Ayurvedic and traditional medicines of Brazil and China. The whole plant and the leaves have many folkloric uses for diverse ailments including pain, heart disease, convulsions, coughs and colds, asthma, bronchitis and tonsillitis among others. This study investigated the phytochemical components, acute toxicity, analgesic and anti-pyretic activities of the aqueous leaf extract of Plectranthus barbatus locally known as Ekizeera in Uganda. The plant leaves were authenticated, collected and decoction was done according to local method. Phytochemical screening was conducted using methods outlined by Trease and Evans and Harborne to determine the components of the extract. Acute toxicity tests were conducted in rats using modified Lorke’s method to determine the safety of the plant material. Analgesic studies were carried out using both a mechanical method (thermally induced pain by tail-flick) and a chemical method (formalin induced pain) in rats by administering extracts orally at 100, 200 and 400 mg/kg of body weight. The method of Al-Ghamdi, modified for local laboratory setting by Adzu was adopted and used for anti-pyretic test. Decoction yielded 9.9% extract. Phytochemical screening confirmed presence of saponins, tannins, alkaloids, terpenoids and essential oils. Acute toxicity tests revealed no deaths in rats after oral treatment with up to 10,000 mg/kg of extract. Tail-flick test was non-significant (p > 0.05) while formalin-induced pain test demonstrated significant activity (p -tests. Anti-pyretic activity was non-significant (p > 0.05) with student t-test. These results suggest that the aqueous leaf extract of Plectranthus barbatus contains specific phytochemicals, has a potent dose dependent analgesic activity, no anti-pyretic activity and can be regarded as a safe medicinal plant to use traditionally, which might further be developed for conventional medical practice.

碰碰香的温室无土栽培技术及开发利用研究

采用无土栽培技术进行碰碰香温室栽培,可解决黑龙江地区冬季不能室外种植的问题。文中综述了碰碰香的无土栽培过程和技术,并介绍了碰碰香相关产品的开发用途,为碰碰香的栽培和产品加工提供一定的参考。