OBJECTIVE The current study was designed to investigate the anti-steatosis effect of Pleurotus citrinopileatus extract(PC) and the underlying mechanism in vivo and in vitro.METHODS Acute and chronic alcoholic hepatost...OBJECTIVE The current study was designed to investigate the anti-steatosis effect of Pleurotus citrinopileatus extract(PC) and the underlying mechanism in vivo and in vitro.METHODS Acute and chronic alcoholic hepatosteatosis murine models and ethanol-treated HepG2 cells were applied.RESULTS In vitro,the anti-steatosis effect of PC was further confirmed via Nile red staining in HepG2 cells treated with ethanol.Both of acute and chronic alcohol-induced mice hepatosteatosis model,PC decreased serum aminotransferase and triglyceride accumulation.Upregulated sterol-regulatory element binding protein1(Srebp1),purinergic ligand-gated ion channel 7 receptor(P2 X7 R) and downregulated sirtuin1(SIRT1),adenosine 5′-monophosphate(AMP)-activated protein kinase α(AMPKα) caused by acute and chronic alcohol intake were modulated by PC.In ethanol-exposed HepG2 cells,PC reduced lipid accumulation in a concentration-dependent manner and exhibited superior ability in controlling lipid accumulation compared with metformin.CONCLUSION PC could abolish hepatic lipid accumulation through regulating SIRT1-AMPKα signaling in acute and chronic alcohol-induced hepatic steatosis.展开更多
In the course to find a stimulating compound for melanin biosynthesis, which should be useful for a gray and a white hair-preventive agent or tanning agent, we evaluated the effects of the methanol extract from mushro...In the course to find a stimulating compound for melanin biosynthesis, which should be useful for a gray and a white hair-preventive agent or tanning agent, we evaluated the effects of the methanol extract from mushroom of Pleurotus citrinopileatus on melanin production in B16 melanoma cells without theophylline. Activity-guided fractionation led to isolate myo-inositol (3) and D-mannitol (4) as the stimulating compounds on melanin production in B16 melanoma cells. Also, ergosterol (1), uracil (2), and D-glucose (5) have been isolated from the methanol extract of P. citrinopileatus and showed no effect on melanin production in B16 melanoma cells. These results indicated that myo-inositol (3) and D-mannitol (4) are potential candidates that could be useful such as a gray and a white hair-preventive agent or tanning agent.展开更多
对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和...对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和芳香族氨基酸总量的比值为2.63;经氨基酸评分(amino acid score,AAS)、化学评分(chemical score,CS)、氨基酸比值系数评分(score of ratio coefficient of amino acid,SRCAA)3种指标评分,其第一限制氨基酸均为胱氨酸+蛋氨酸(Cys+Met);以FAO/WHO模式评价,桑枝榆黄蘑子实体所含蛋白质是优质蛋白,且营养价值较高。展开更多
基金supported by National Natural Science Foundation of China (81560597 81260664+1 种基金 81360658 81660689)
文摘OBJECTIVE The current study was designed to investigate the anti-steatosis effect of Pleurotus citrinopileatus extract(PC) and the underlying mechanism in vivo and in vitro.METHODS Acute and chronic alcoholic hepatosteatosis murine models and ethanol-treated HepG2 cells were applied.RESULTS In vitro,the anti-steatosis effect of PC was further confirmed via Nile red staining in HepG2 cells treated with ethanol.Both of acute and chronic alcohol-induced mice hepatosteatosis model,PC decreased serum aminotransferase and triglyceride accumulation.Upregulated sterol-regulatory element binding protein1(Srebp1),purinergic ligand-gated ion channel 7 receptor(P2 X7 R) and downregulated sirtuin1(SIRT1),adenosine 5′-monophosphate(AMP)-activated protein kinase α(AMPKα) caused by acute and chronic alcohol intake were modulated by PC.In ethanol-exposed HepG2 cells,PC reduced lipid accumulation in a concentration-dependent manner and exhibited superior ability in controlling lipid accumulation compared with metformin.CONCLUSION PC could abolish hepatic lipid accumulation through regulating SIRT1-AMPKα signaling in acute and chronic alcohol-induced hepatic steatosis.
文摘In the course to find a stimulating compound for melanin biosynthesis, which should be useful for a gray and a white hair-preventive agent or tanning agent, we evaluated the effects of the methanol extract from mushroom of Pleurotus citrinopileatus on melanin production in B16 melanoma cells without theophylline. Activity-guided fractionation led to isolate myo-inositol (3) and D-mannitol (4) as the stimulating compounds on melanin production in B16 melanoma cells. Also, ergosterol (1), uracil (2), and D-glucose (5) have been isolated from the methanol extract of P. citrinopileatus and showed no effect on melanin production in B16 melanoma cells. These results indicated that myo-inositol (3) and D-mannitol (4) are potential candidates that could be useful such as a gray and a white hair-preventive agent or tanning agent.
文摘对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和芳香族氨基酸总量的比值为2.63;经氨基酸评分(amino acid score,AAS)、化学评分(chemical score,CS)、氨基酸比值系数评分(score of ratio coefficient of amino acid,SRCAA)3种指标评分,其第一限制氨基酸均为胱氨酸+蛋氨酸(Cys+Met);以FAO/WHO模式评价,桑枝榆黄蘑子实体所含蛋白质是优质蛋白,且营养价值较高。
