The Indian meal moth,Plodia interpunctella(Lepidoptera:Pyralidae),a globally distributed storage pest,relies on odors that are emitted from stored foods to select a suitable substrate for oviposition.However,the molec...The Indian meal moth,Plodia interpunctella(Lepidoptera:Pyralidae),a globally distributed storage pest,relies on odors that are emitted from stored foods to select a suitable substrate for oviposition.However,the molecular mechanism underlying the chemical communication between P.interpunctella and its host remains elusive.In this study,130 chemosensory genes were identified from the transcriptomes of 7 P.interpunctella tissues,and the quantitative expression levels of all 56 P.interpunctella odorant receptor genes(PintORs)were validated using real-time quantitative polymerase chain reaction.The functional characteristics of 5 PintORs with female antennae-biased expression were investigated using 2-electrode voltage clamp recordings in Xenopus laevis oocytes.PintOR23 was found to be specifically tuned to acetophenone.Acetophenone could elicit a significant electrophysiological response and only attracted mated females when compared with males and virgin females.In addition,molecular docking predicted that the hydrogen bonding sites,TRP-335 and ALA-167,might play key roles in the binding of PintOR23 to acetophenone.Our study provides valuable insights into the olfactory mechanism of oviposition substrate detection and localization in P.interpunctella and points toward the possibility of developing eco-friendly odorant agents to control pests of stored products.展开更多
The suitability of combining microbial pesticides and an insect parasitoid for pest management of stored cereal in China was evaluated using laboratory assays. For this purpose, interactions between Bacillus thuringie...The suitability of combining microbial pesticides and an insect parasitoid for pest management of stored cereal in China was evaluated using laboratory assays. For this purpose, interactions between Bacillus thuringiensis （Bt）, Bt-intoxicated host larvae and the parasitoid Habrobracon hebetor （Say） （Hymenoptera： Braconidae） were tested against Plodia interpunctella Hubner （Lepidoptera： Pyralidae）. Bt or H. hebetor alone caused 41.67% and 35.35% P interpunctella larval mortality respectively. The Bt- parasitoid combined treatment significantly increased mortality ofP interpunctella （86%）. Progeny development of H. hebetor was dependent upon its susceptibility to Bt. Fewer parasitoids emerged from Bt-parasitoid combined treatment than in non-Bt treatments. However, since Bt did not prevent parasitoid development, a combined treatment with Bt and parasitoid release could produce better protection against P. interpunctella than either treatments when used singly, because their lethal effects were additive to each other.展开更多
A field experiment of 4 years＇ duration was carried out to evaluate the ef- ficacy of combining the mating disruption （MD） formulation Dismate ZETA （（9Z,12E）- tetradecadienyl acetate）, with the parasitoid Habro...A field experiment of 4 years＇ duration was carried out to evaluate the ef- ficacy of combining the mating disruption （MD） formulation Dismate ZETA （（9Z,12E）- tetradecadienyl acetate）, with the parasitoid Habrobracon hebetor against the Indianmeal moth Plodia interpunctella in a chocolate factory. The experimental period began early in 2011 and ended in late 2014. Begane Dismate dispensers were placed in the facility from 2011 to 2014 and H. hebetor was released in 2014. Pheromone-baited traps were used to monitor the flight activity of the male moths and oviposition Petri dish traps were placed to assess the progeny production ofP. interpunctella females. Following the start of MD, a decrease in the number ofP. interpunctella males caught in monitoring traps was observed from 2011 to 2013. A further decline in the moth population was noted in 2014, when MD was combined with the release of parasitoids. The presence of larvae in the oviposition cups was occasionally observed throughout the monitoring period, from 2011 to 2014. This study demonstrates that the combined system of MD and parasitoids is an effective and reliable technique that can be used to successfully control P. interpunctella.展开更多
Aerosol insecticides may provide an alternative to fumigants for control of the Indianmeal moth, Plodia interpunctella （Hübner）, the Indianmeal moth, a major insect pest of stored processed food. In this study,...Aerosol insecticides may provide an alternative to fumigants for control of the Indianmeal moth, Plodia interpunctella （Hübner）, the Indianmeal moth, a major insect pest of stored processed food. In this study, eggs and larvae （5th instars） ofP. interpunctella were exposed to aerosol applications of the pyrethroid esfenvalerate and insect growth regulator methoprene, alone and in combination, in open and obstructed positions inside small sheds. When larvae were exposed to methoprene alone, adult emergence from those exposed larvae was 7.1% ± 1.5%. In contrast, adult emergence was 92.5% ± 3.5% when larvae were exposed to esfenvalerate alone. When eggs were exposed to methoprene, adult emergence of those exposed eggs was approximately 75%; however, when eggs were exposed to esfenvalerate, adult emergence was approximately 35%. In the combination treatment of methoprene plus esfenvalerate at their respective label rates, adult emergence following larval exposure was 0.91% ±0.61% compared to 16.3% ± 9.6% when eggs were exposed. Based on our results, methoprene alone is highly effective in reducing adult emergence after larval exposure. However, it is not as effective on eggs as esfenvalerate. A combination treatment of esfenvalerate plus methoprene could be used to control eggs and the wandering-phase larval stages ofP. interpunctella. An economic risk analysis also supports a strategy of combining methoprene and esfenvalerate.展开更多
基金This research was supported by the China Postdoctoral Science Foundation(2021M690588)the Fundamental Research Funds for the Central Universities(135111010)+1 种基金the Young Science and Technology Talent Support Project of Jilin Province(QT202121)The authors would like to thank all the reviewers who participated in the review,as well as MJEditor(www.mjeditor.com)for providing English editing services during the preparation of this manuscript.
文摘The Indian meal moth,Plodia interpunctella(Lepidoptera:Pyralidae),a globally distributed storage pest,relies on odors that are emitted from stored foods to select a suitable substrate for oviposition.However,the molecular mechanism underlying the chemical communication between P.interpunctella and its host remains elusive.In this study,130 chemosensory genes were identified from the transcriptomes of 7 P.interpunctella tissues,and the quantitative expression levels of all 56 P.interpunctella odorant receptor genes(PintORs)were validated using real-time quantitative polymerase chain reaction.The functional characteristics of 5 PintORs with female antennae-biased expression were investigated using 2-electrode voltage clamp recordings in Xenopus laevis oocytes.PintOR23 was found to be specifically tuned to acetophenone.Acetophenone could elicit a significant electrophysiological response and only attracted mated females when compared with males and virgin females.In addition,molecular docking predicted that the hydrogen bonding sites,TRP-335 and ALA-167,might play key roles in the binding of PintOR23 to acetophenone.Our study provides valuable insights into the olfactory mechanism of oviposition substrate detection and localization in P.interpunctella and points toward the possibility of developing eco-friendly odorant agents to control pests of stored products.
文摘The suitability of combining microbial pesticides and an insect parasitoid for pest management of stored cereal in China was evaluated using laboratory assays. For this purpose, interactions between Bacillus thuringiensis （Bt）, Bt-intoxicated host larvae and the parasitoid Habrobracon hebetor （Say） （Hymenoptera： Braconidae） were tested against Plodia interpunctella Hubner （Lepidoptera： Pyralidae）. Bt or H. hebetor alone caused 41.67% and 35.35% P interpunctella larval mortality respectively. The Bt- parasitoid combined treatment significantly increased mortality ofP interpunctella （86%）. Progeny development of H. hebetor was dependent upon its susceptibility to Bt. Fewer parasitoids emerged from Bt-parasitoid combined treatment than in non-Bt treatments. However, since Bt did not prevent parasitoid development, a combined treatment with Bt and parasitoid release could produce better protection against P. interpunctella than either treatments when used singly, because their lethal effects were additive to each other.
文摘A field experiment of 4 years＇ duration was carried out to evaluate the ef- ficacy of combining the mating disruption （MD） formulation Dismate ZETA （（9Z,12E）- tetradecadienyl acetate）, with the parasitoid Habrobracon hebetor against the Indianmeal moth Plodia interpunctella in a chocolate factory. The experimental period began early in 2011 and ended in late 2014. Begane Dismate dispensers were placed in the facility from 2011 to 2014 and H. hebetor was released in 2014. Pheromone-baited traps were used to monitor the flight activity of the male moths and oviposition Petri dish traps were placed to assess the progeny production ofP. interpunctella females. Following the start of MD, a decrease in the number ofP. interpunctella males caught in monitoring traps was observed from 2011 to 2013. A further decline in the moth population was noted in 2014, when MD was combined with the release of parasitoids. The presence of larvae in the oviposition cups was occasionally observed throughout the monitoring period, from 2011 to 2014. This study demonstrates that the combined system of MD and parasitoids is an effective and reliable technique that can be used to successfully control P. interpunctella.
文摘Aerosol insecticides may provide an alternative to fumigants for control of the Indianmeal moth, Plodia interpunctella （Hübner）, the Indianmeal moth, a major insect pest of stored processed food. In this study, eggs and larvae （5th instars） ofP. interpunctella were exposed to aerosol applications of the pyrethroid esfenvalerate and insect growth regulator methoprene, alone and in combination, in open and obstructed positions inside small sheds. When larvae were exposed to methoprene alone, adult emergence from those exposed larvae was 7.1% ± 1.5%. In contrast, adult emergence was 92.5% ± 3.5% when larvae were exposed to esfenvalerate alone. When eggs were exposed to methoprene, adult emergence of those exposed eggs was approximately 75%; however, when eggs were exposed to esfenvalerate, adult emergence was approximately 35%. In the combination treatment of methoprene plus esfenvalerate at their respective label rates, adult emergence following larval exposure was 0.91% ±0.61% compared to 16.3% ± 9.6% when eggs were exposed. Based on our results, methoprene alone is highly effective in reducing adult emergence after larval exposure. However, it is not as effective on eggs as esfenvalerate. A combination treatment of esfenvalerate plus methoprene could be used to control eggs and the wandering-phase larval stages ofP. interpunctella. An economic risk analysis also supports a strategy of combining methoprene and esfenvalerate.
