Fertile, advanced generation hybrids derived from crosses between Texas (Poa arachnifera Torr.) and Kentucky (Poa pratensis L.) bluegrass have been selected. The hybrids are currently being evaluated for low-input tur...Fertile, advanced generation hybrids derived from crosses between Texas (Poa arachnifera Torr.) and Kentucky (Poa pratensis L.) bluegrass have been selected. The hybrids are currently being evaluated for low-input turf potential. Since they are derived from hand-harvested seed from first-generation hybrids located in field nurseries their exact genetic origin is unknown. This experiment was conducted to determine if there are still genetic relationships among the advanced generation hybrids and some of the Texas and Kentucky parents in their pedigrees. Four Inter Simple Sequence Repeat (ISSR) primer combinations resolved on 6% nondenaturing polyacrylamide gels resulted in 179 polymorphic bands that were scored to create a genetic similarity matrix and dendrogram based on Jaccard’s coefficient. The clustering of the advanced generation hybrids was generally in agreement with what would be expected based on their pedigrees and indicated it was more likely to select a fertile hybrid from an advanced generation, rather than the F1 generation.展开更多
High embryogenic calli of three cutivars of Kentucky bluegrass, Md, Bd, and Gm, were induced from mature embryos, and were proliferated on medium K3 and K5. Embryogenic calli were transformed with plamids pDM803 and p...High embryogenic calli of three cutivars of Kentucky bluegrass, Md, Bd, and Gm, were induced from mature embryos, and were proliferated on medium K3 and K5. Embryogenic calli were transformed with plamids pDM803 and pBY520 by microprojectile bombardment. Fourty-two transgenic lines had been obtained. The highest efficiency of transformation reached to 3.7% for cv. Md, 2.8% for cv. Gm, and 5 % for cv. Bd. The micro nutriment of Cupric had significant effect on transformation. The embryogenic callus cultured in dim-light condition had higher transformation efficiency than the green callus cultured in light condition for one month before transformation. The selective regime and selective pressure on the putative transgenic plants were important for obtaining the desire number of transgenic plants. It also affected the copy number of integrated genes in the genomic DNA of transgenic plants.展开更多
Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drou...Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drought resistance gene from GenBank. The amplified products were positive recon identified by using procedures of recovery, connection, transformation and enzyme separation. The length of cloned gene sequence was 324 bp, identity reached 79.27% with Barley HVA1 gene that meaned the cloned gene sequence was the partial HVA1 gene of Poapratensis.展开更多
<i><span style="font-family:"">Poa</span></i><span style="font-family:""> <i>pratensis</i> </span><span style="font-family:"&q...<i><span style="font-family:"">Poa</span></i><span style="font-family:""> <i>pratensis</i> </span><span style="font-family:"">L. (Poaceae) is a hardy, persistent, attractive forage and turf grass adapted to a wide range of soils and climate. In this study, we release and detail the complete chloroplast genome sequences of <i>P</i>. <i>pratensis</i>. The whole chloroplast genome was 135,649 bp in length and comprised 131 genes, including 85 protein-coding genes, 38 tRNA genes, 8 rRNA genes. The <i>P</i>. <i>pratensis</i> chloroplast genome had a GC content of 38.3%. The result of phylogenetic analysis showed that <i>P</i>. <i>pratensis</i> was closely related to <i>P</i>. <i>pratensis</i> cv. <i>Qinghai</i> and <i>P</i>. <i>poophagorum</i>. This study would provide useful genetic information for the protection of <i>P</i>. <i>pratensis</i> and other related species.展开更多
A protocol was discussed for high efficient plant regeneration from seven bluegrass （Poa pratensis L.） cultivars via an in- direct callus induction and somatic embryogenesis method. Mature seeds were used as explant...A protocol was discussed for high efficient plant regeneration from seven bluegrass （Poa pratensis L.） cultivars via an in- direct callus induction and somatic embryogenesis method. Mature seeds were used as explants for callus initiation. Callus induction and proliferation efficiencies were investigated on NB, modified MS （MMS） and MS media, supplemented with 2.0 mg·L^-1 2,4-dichlorophenoxyacetic acid （2,4-D）. The MMS medium performed best. Based on the MMS medium, direct and indirect callus induction effects of bluegrass from mature seeds were compared at the range of 1-5 mg·L^-1 2,4-D contained in the medium. Under the direct callus induction method, the most suitable 2,4-D concentrations varied among cultivars. Under the indirect callus induction method, a significantly high callus induction frequency （93.33%-98.33%） was obtained and there were barely any statistically sig- nificant differences among the tested genetically diverse cultivars. Somatic embryos were promoted on the MMS medium supple- mented with 3 mg·L^-1 2,4-D, 0.1 mg·L^-1 kinetin and 0.8 mg·L^-1 CuSO4. Embryogenetic calli developed into plantlets on the MMS medium containing different concentrations of thidiazuron （TDZ）, and the differentiation frequencies varied in the range from 20.15% to 77.65%. The 0.25 mg·L^-1 TDZ was generally the most suitable concentration for the tested cultivars.展开更多
Objective] The aim was to explain the accumulation characteristic of mineral elements in alpine grassland plants and the effect of supplementary supply on the nutrient changes of mineral elements. [Method] Potted Poa ...Objective] The aim was to explain the accumulation characteristic of mineral elements in alpine grassland plants and the effect of supplementary supply on the nutrient changes of mineral elements. [Method] Potted Poa crymophila cv. Qinghai plants were regularly applied with liquid mineral mixed fertilizers, and samples were collected for analysis and detection. [Result] After the regular spraying of liquid mineral mixed fertilizers, the mineral elements in potted P. crymophila and its soil reduced （ P 〈0.05）, but the accumulation of corresponding mineral elements of P. crymophila in the control group was increased due to the ＂Starvation Effect＂, which was one of the endogenetic forces driving the increase in the accumulation of mineral elements. [Conclusion] This study is of great significance for the study of the accumulation of mineral elements in degraded grassland plants and its dynamic mechanism, as well as testing the hypothesis of the Starvation Effect of mineral elements. In addition, it also provides scientific bases and technical support for the restoration and remediation of degraded grassland and the development of ecological livestock husbandry.展开更多
为探究草地早熟禾无融合生殖胚囊发育过程中基因表达谱的变化,本研究利用高通量Illu-mina Hiseq测序技术对‘清水’草地早熟禾无融合生殖子房发育的无孢子起始细胞发生时期(8DBA)和无孢子生殖胚囊发育时期(6DBA、4DBA和2DBA)共4个阶段...为探究草地早熟禾无融合生殖胚囊发育过程中基因表达谱的变化,本研究利用高通量Illu-mina Hiseq测序技术对‘清水’草地早熟禾无融合生殖子房发育的无孢子起始细胞发生时期(8DBA)和无孢子生殖胚囊发育时期(6DBA、4DBA和2DBA)共4个阶段的小穗进行转录组测序。结果显示,不同差异分组(2DBA vs 4DBA、2DBA vs6DBA和2DBA vs 8DBA)共有13575个DEGs,包括7191个上调基因和6384个下调基因。GO富集分析发现,3个差异分组的DEGs显著富集在过氧化氢的响应、蛋白质重折叠、营养水库活动、错误折叠蛋白结合和双糖生物合成等过程中。KEGG通路分析表明,3个差异分组的DEGs显著富集在代谢途径、次生代谢产物的生物合成、淀粉和蔗糖代谢及内质网中的蛋白加工等通路上,在2DBA vs 8DBA分组中发现植物激素信号转导途径的富集。4个时期占比最多的转录因子家族是NAC、AP2/ERF-ERF和MYB-related等。将在4个发育阶段特异性高表达的基因OPR1、ASN2、SAMS2、HSP81-2、PXG4和FPK2作为与‘清水’草地早熟禾无融合生殖胚囊发育相关的候选基因。qRTPCR分析表明,除WOX2在4DBA上调,与RNA-seq的表达存在差异外,其余16个DEGs的表达量变化趋势与高通量测序结果基本一致。展开更多
文摘Fertile, advanced generation hybrids derived from crosses between Texas (Poa arachnifera Torr.) and Kentucky (Poa pratensis L.) bluegrass have been selected. The hybrids are currently being evaluated for low-input turf potential. Since they are derived from hand-harvested seed from first-generation hybrids located in field nurseries their exact genetic origin is unknown. This experiment was conducted to determine if there are still genetic relationships among the advanced generation hybrids and some of the Texas and Kentucky parents in their pedigrees. Four Inter Simple Sequence Repeat (ISSR) primer combinations resolved on 6% nondenaturing polyacrylamide gels resulted in 179 polymorphic bands that were scored to create a genetic similarity matrix and dendrogram based on Jaccard’s coefficient. The clustering of the advanced generation hybrids was generally in agreement with what would be expected based on their pedigrees and indicated it was more likely to select a fertile hybrid from an advanced generation, rather than the F1 generation.
基金This project is supported by the Foundation of State Key Laboratory of Freshwater Ecology and Biotechnology,China(2001FB10)Young Foundation of Shanxi,China(20001038)+2 种基金Shanxi Scholarship Foundation,Chinathe Hational Natural Science Foundation of China(30270204)DLF-Trifolium,Research Division,Denmark.
文摘High embryogenic calli of three cutivars of Kentucky bluegrass, Md, Bd, and Gm, were induced from mature embryos, and were proliferated on medium K3 and K5. Embryogenic calli were transformed with plamids pDM803 and pBY520 by microprojectile bombardment. Fourty-two transgenic lines had been obtained. The highest efficiency of transformation reached to 3.7% for cv. Md, 2.8% for cv. Gm, and 5 % for cv. Bd. The micro nutriment of Cupric had significant effect on transformation. The embryogenic callus cultured in dim-light condition had higher transformation efficiency than the green callus cultured in light condition for one month before transformation. The selective regime and selective pressure on the putative transgenic plants were important for obtaining the desire number of transgenic plants. It also affected the copy number of integrated genes in the genomic DNA of transgenic plants.
基金Supported by Committee of Nature Science Foundation of Heilongjiang Province (C0207)
文摘Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drought resistance gene from GenBank. The amplified products were positive recon identified by using procedures of recovery, connection, transformation and enzyme separation. The length of cloned gene sequence was 324 bp, identity reached 79.27% with Barley HVA1 gene that meaned the cloned gene sequence was the partial HVA1 gene of Poapratensis.
文摘<i><span style="font-family:"">Poa</span></i><span style="font-family:""> <i>pratensis</i> </span><span style="font-family:"">L. (Poaceae) is a hardy, persistent, attractive forage and turf grass adapted to a wide range of soils and climate. In this study, we release and detail the complete chloroplast genome sequences of <i>P</i>. <i>pratensis</i>. The whole chloroplast genome was 135,649 bp in length and comprised 131 genes, including 85 protein-coding genes, 38 tRNA genes, 8 rRNA genes. The <i>P</i>. <i>pratensis</i> chloroplast genome had a GC content of 38.3%. The result of phylogenetic analysis showed that <i>P</i>. <i>pratensis</i> was closely related to <i>P</i>. <i>pratensis</i> cv. <i>Qinghai</i> and <i>P</i>. <i>poophagorum</i>. This study would provide useful genetic information for the protection of <i>P</i>. <i>pratensis</i> and other related species.
文摘A protocol was discussed for high efficient plant regeneration from seven bluegrass （Poa pratensis L.） cultivars via an in- direct callus induction and somatic embryogenesis method. Mature seeds were used as explants for callus initiation. Callus induction and proliferation efficiencies were investigated on NB, modified MS （MMS） and MS media, supplemented with 2.0 mg·L^-1 2,4-dichlorophenoxyacetic acid （2,4-D）. The MMS medium performed best. Based on the MMS medium, direct and indirect callus induction effects of bluegrass from mature seeds were compared at the range of 1-5 mg·L^-1 2,4-D contained in the medium. Under the direct callus induction method, the most suitable 2,4-D concentrations varied among cultivars. Under the indirect callus induction method, a significantly high callus induction frequency （93.33%-98.33%） was obtained and there were barely any statistically sig- nificant differences among the tested genetically diverse cultivars. Somatic embryos were promoted on the MMS medium supple- mented with 3 mg·L^-1 2,4-D, 0.1 mg·L^-1 kinetin and 0.8 mg·L^-1 CuSO4. Embryogenetic calli developed into plantlets on the MMS medium containing different concentrations of thidiazuron （TDZ）, and the differentiation frequencies varied in the range from 20.15% to 77.65%. The 0.25 mg·L^-1 TDZ was generally the most suitable concentration for the tested cultivars.
基金Supported by the General Program of Natural Science Foundation of Qinghai Province(2016-ZJ-906)
文摘Objective] The aim was to explain the accumulation characteristic of mineral elements in alpine grassland plants and the effect of supplementary supply on the nutrient changes of mineral elements. [Method] Potted Poa crymophila cv. Qinghai plants were regularly applied with liquid mineral mixed fertilizers, and samples were collected for analysis and detection. [Result] After the regular spraying of liquid mineral mixed fertilizers, the mineral elements in potted P. crymophila and its soil reduced （ P 〈0.05）, but the accumulation of corresponding mineral elements of P. crymophila in the control group was increased due to the ＂Starvation Effect＂, which was one of the endogenetic forces driving the increase in the accumulation of mineral elements. [Conclusion] This study is of great significance for the study of the accumulation of mineral elements in degraded grassland plants and its dynamic mechanism, as well as testing the hypothesis of the Starvation Effect of mineral elements. In addition, it also provides scientific bases and technical support for the restoration and remediation of degraded grassland and the development of ecological livestock husbandry.
文摘为探究草地早熟禾无融合生殖胚囊发育过程中基因表达谱的变化,本研究利用高通量Illu-mina Hiseq测序技术对‘清水’草地早熟禾无融合生殖子房发育的无孢子起始细胞发生时期(8DBA)和无孢子生殖胚囊发育时期(6DBA、4DBA和2DBA)共4个阶段的小穗进行转录组测序。结果显示,不同差异分组(2DBA vs 4DBA、2DBA vs6DBA和2DBA vs 8DBA)共有13575个DEGs,包括7191个上调基因和6384个下调基因。GO富集分析发现,3个差异分组的DEGs显著富集在过氧化氢的响应、蛋白质重折叠、营养水库活动、错误折叠蛋白结合和双糖生物合成等过程中。KEGG通路分析表明,3个差异分组的DEGs显著富集在代谢途径、次生代谢产物的生物合成、淀粉和蔗糖代谢及内质网中的蛋白加工等通路上,在2DBA vs 8DBA分组中发现植物激素信号转导途径的富集。4个时期占比最多的转录因子家族是NAC、AP2/ERF-ERF和MYB-related等。将在4个发育阶段特异性高表达的基因OPR1、ASN2、SAMS2、HSP81-2、PXG4和FPK2作为与‘清水’草地早熟禾无融合生殖胚囊发育相关的候选基因。qRTPCR分析表明,除WOX2在4DBA上调,与RNA-seq的表达存在差异外,其余16个DEGs的表达量变化趋势与高通量测序结果基本一致。
