2种野生岩生植物叶片可溶性蛋白含量对低温胁迫的响应

[目的]研究植物叶片可溶性蛋白质含量与植物抗寒性的关系。[方法]以野生岩生植物金发草和丛毛羊胡子草作为研究材料,并以目前最常用的护坡植物狗牙根作为对照,研究低温胁迫下2种野生岩生植物叶片可溶性蛋白含量的变化情况,并对蛋白质进行SDS-PAGE分析。[结果]结果表明:在低温胁迫下,丛毛羊胡子草、金发草和狗牙根的可溶性蛋白含量随处理温度的降低而增大;在各处理温度下丛毛羊胡子草可溶性蛋白含量均明显高于金发草和狗牙根,并且3种草可溶性蛋白含量12月高于8月;3种草12月蛋白质SDS-PAGE分析表明,丛毛羊胡子草蛋白质条带数最多且条带着色深,金发草次之,狗牙根蛋白质条带数最少且条带着色浅。[结论]3种草种中,丛毛羊胡子草具有较强的抗寒性。

两种野生岩生植物的抗旱适应性研究

采用盆栽试验研究干旱胁迫下两种野生岩生植物金发草[Pogonatherumpanideum(Lam.)Hack]、丛毛羊胡子草(ErioophorumcomosumNees)的抗旱形态、生理表现。结果表明随着干旱胁迫程度加强,两种野生岩生植物的叶片相对电导率增大;胁迫初期可溶性蛋白含量小幅度增加,随后呈缓慢下降;金发草丙二醛(MDA)含量呈上升趋势,羊胡子草则先降后升;金发草可溶性糖含量先升后降,羊胡子草则一直呈现缓慢上升趋势;游离脯氨酸(Pro)含量均呈先升后降趋势。综合各项指标,丛毛羊胡子草抗旱性强于金发草。

两种岩生植物种子鉴定及播种量研究

以两种岩生植物金发草和丛毛羊胡子草为研究对象,进行了种子鉴定与播种量分析研究,结果表明:两种岩生植物具有种子产业化研究价值;两种岩生植物的合理播种量为:金发草0.3043￣0.4226g/m2,丛毛羊胡子草0.2722￣0.3778g/m2。

Comparison of Drought-Resistance about Three Wild Rocky-Grasses

In this experiment, using the methods of drought-stress in the pot and PEG simulative drought-stress, three native rocky-slope grasses Pogonatherum panideum（Lam.） Hack, Erioophorum comosum nees and Cynodon dactylon cultivated in the pots were selected as materials to study their drought-resistance by analyzing the indexes such as the leaf area index, the drying roots accumulation, the holding water ability of leaf, the relative conductance of leaf, the soluble protein, chlorophyll. At the same time, by comparing with membership function value of each index, the order of each grass drought-resistance was decided. The results showed that the drought-resistance of Erioophorum comosum nees was stronger than Cynodon dactylon and drought-resistance of Cynodon dactylon was stronger than Pogonatherum panideum （Lam.） Hack. The purpose of the experimental results was to find theoretical foundations for selecting and cultivating native protecting-slope vegetations to adapt to rocky slopes.