The supercritical carbon dioxide extraction was applied to obtain essential oil from Pogostemon cablin in this work.Effect of extraction parameters including temperature,pressure,extraction time and particle size on e...The supercritical carbon dioxide extraction was applied to obtain essential oil from Pogostemon cablin in this work.Effect of extraction parameters including temperature,pressure,extraction time and particle size on extraction yield was investigated,and the response surface methodology with a Box–Behnken Design was used to achieve the optimized extraction conditions.The maximum yield of essential oil was 2.4356%under the conditions of extraction temperature 47°C,pressure 24.5 MPa and extraction time 119 min.Moreover,based on the Brunauer–Emmett–Teller theory of adsorption,a mathematical modeling was performed to correlate the measured data.The model shows a function relationship between extraction yield and time by a simple equation with three significantly adjustable parameters.These model parameters have been optimized through simulated annealing algorithm.The predicted data from the mathematical model show a good agreement with the experimental data of the different extraction parameters.展开更多
Pogostemon cablin essential oil(PEO),extracted from P.cablin,has anti-oxidant,anti-inflammatory,and anti-stress properties,as well as the ability to improve gastrointestinal digestion.This study aims to evaluate the e...Pogostemon cablin essential oil(PEO),extracted from P.cablin,has anti-oxidant,anti-inflammatory,and anti-stress properties,as well as the ability to improve gastrointestinal digestion.This study aims to evaluate the effects of PEO on the performance,rumen epithelial morphology,and barrier function in heat-stressed beef cattle.Thirty-six male Jingjiang cattle at 18 months old were randomly assigned into four groups and fed a diet containing PEO at 0(control),50,100,or 150 mg/kg in the feed concentrate(n=9).All experimental cattle were fed under high temperature and humidity in summer for 60 days.The results indicated that 50 mg/kg of PEO treatment enhanced the average daily gain of beef cattle compared with the control group(P=0.032).All PEO treatments reduced the diamine oxidase activity(P=0.004)and malondialdehyde content(P=0.008)in serum.In addition,the content of 70 kDa heat shock protein in the 100 mg/kg group was increased,and the activity of glutathione peroxidase and total antioxidant capacity in both 100 mg/kg and 150 mg/kg groups were enhanced compared to the control group(P<0.05).More importantly,PEO treatment with 50 mg/kg enhanced the mRNA relative expressions of occludin in ruminal epithelia but decreased the mRNA relative expressions of c-Jun N-terminal kinase,P38 mitogen-activated protein kinases,caspase-3,Beclin1(P<0.05),and extremely significant declined the mRNA relative expressions of extracellular regulated protein kinases and ubiquitin-binding protein in contrast to the control group(P<0.01).These findings indicated that dietary PEO supplementation might be favorable to improve growth performance and repairing damaged rumen epithelium of heat-stressed cattle by down-regulating the mitogen-activated protein kinase signaling pathway.展开更多
目的基于液相色谱-串联质谱联用(liquid chromatography-tandem mass spectormetry,LC-MS/MS)技术研究广藿香对贵州小型猪血清代谢的影响,探讨其药理学作用机制。方法将9头贵州小型猪随机分为广藿香给药组(5头)和对照组(4头)。广藿香给...目的基于液相色谱-串联质谱联用(liquid chromatography-tandem mass spectormetry,LC-MS/MS)技术研究广藿香对贵州小型猪血清代谢的影响,探讨其药理学作用机制。方法将9头贵州小型猪随机分为广藿香给药组(5头)和对照组(4头)。广藿香给药组按0.5 g·头-1·d^(-1)剂量通过拌料经口饲喂广藿香中药配方颗粒,连续给药8 d;对照组正常饲喂,不额外给药。用药结束后采集各组动物的血清样品,采用LC-MS/MS技术分析各组样品的血清代谢物,对代谢组数据进行注释并与KEGG、HMDB和Lipidmaps数据库比对,通过偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)、组间聚类、差异代谢物分析和功能富集等生物信息学分析方法,筛选差异代谢生物标志物及其可能的代谢通路。结果在广藿香给药组和对照组的血清样本中共鉴定出443个血清代谢物,筛选出44个差异显著(P<0.05)的代谢物,其中显著上调(P<0.01)的代谢物为肉桂酰甘氨酸、N-苄基-N-异丙基-N'-(4-三氟甲基)苯尿素、亚牛磺酸、D-葡萄糖-6-磷酸、顺-2-癸烯酸、二十碳二烯酸(顺-11,14)、前列腺素A2和10-羟基癸烯酸,显著下调(P<0.01)的代谢物为溶血磷脂酰胆碱22∶5、溶血磷脂酸22∶6和溶血磷脂酸22∶5。差异代谢物主要富集在丙氨酸、天冬氨酸和谷氨酸代谢通路(MapID:map00250),以及牛磺酸和亚牛磺酸代谢通路(MapID:map00430)。结论广藿香能显著影响贵州小型猪的溶血磷脂酸类物质的代谢,可能通过影响天冬氨酸-谷氨酸代谢通路、牛磺酸-亚牛磺酸代谢通路,起到缓解机体氨基酸代谢紊乱、调节炎性反应发生等功能。展开更多
文摘The supercritical carbon dioxide extraction was applied to obtain essential oil from Pogostemon cablin in this work.Effect of extraction parameters including temperature,pressure,extraction time and particle size on extraction yield was investigated,and the response surface methodology with a Box–Behnken Design was used to achieve the optimized extraction conditions.The maximum yield of essential oil was 2.4356%under the conditions of extraction temperature 47°C,pressure 24.5 MPa and extraction time 119 min.Moreover,based on the Brunauer–Emmett–Teller theory of adsorption,a mathematical modeling was performed to correlate the measured data.The model shows a function relationship between extraction yield and time by a simple equation with three significantly adjustable parameters.These model parameters have been optimized through simulated annealing algorithm.The predicted data from the mathematical model show a good agreement with the experimental data of the different extraction parameters.
基金The National Natural Science Foundation of China (32060768)Central guidance for local scientifc and technological development funding projects of China (20231zdf03083)the China Agriculture Research System of MOF and MARA (CARS-37)provided financial support for this study.
文摘Pogostemon cablin essential oil(PEO),extracted from P.cablin,has anti-oxidant,anti-inflammatory,and anti-stress properties,as well as the ability to improve gastrointestinal digestion.This study aims to evaluate the effects of PEO on the performance,rumen epithelial morphology,and barrier function in heat-stressed beef cattle.Thirty-six male Jingjiang cattle at 18 months old were randomly assigned into four groups and fed a diet containing PEO at 0(control),50,100,or 150 mg/kg in the feed concentrate(n=9).All experimental cattle were fed under high temperature and humidity in summer for 60 days.The results indicated that 50 mg/kg of PEO treatment enhanced the average daily gain of beef cattle compared with the control group(P=0.032).All PEO treatments reduced the diamine oxidase activity(P=0.004)and malondialdehyde content(P=0.008)in serum.In addition,the content of 70 kDa heat shock protein in the 100 mg/kg group was increased,and the activity of glutathione peroxidase and total antioxidant capacity in both 100 mg/kg and 150 mg/kg groups were enhanced compared to the control group(P<0.05).More importantly,PEO treatment with 50 mg/kg enhanced the mRNA relative expressions of occludin in ruminal epithelia but decreased the mRNA relative expressions of c-Jun N-terminal kinase,P38 mitogen-activated protein kinases,caspase-3,Beclin1(P<0.05),and extremely significant declined the mRNA relative expressions of extracellular regulated protein kinases and ubiquitin-binding protein in contrast to the control group(P<0.01).These findings indicated that dietary PEO supplementation might be favorable to improve growth performance and repairing damaged rumen epithelium of heat-stressed cattle by down-regulating the mitogen-activated protein kinase signaling pathway.
文摘目的基于液相色谱-串联质谱联用(liquid chromatography-tandem mass spectormetry,LC-MS/MS)技术研究广藿香对贵州小型猪血清代谢的影响,探讨其药理学作用机制。方法将9头贵州小型猪随机分为广藿香给药组(5头)和对照组(4头)。广藿香给药组按0.5 g·头-1·d^(-1)剂量通过拌料经口饲喂广藿香中药配方颗粒,连续给药8 d;对照组正常饲喂,不额外给药。用药结束后采集各组动物的血清样品,采用LC-MS/MS技术分析各组样品的血清代谢物,对代谢组数据进行注释并与KEGG、HMDB和Lipidmaps数据库比对,通过偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)、组间聚类、差异代谢物分析和功能富集等生物信息学分析方法,筛选差异代谢生物标志物及其可能的代谢通路。结果在广藿香给药组和对照组的血清样本中共鉴定出443个血清代谢物,筛选出44个差异显著(P<0.05)的代谢物,其中显著上调(P<0.01)的代谢物为肉桂酰甘氨酸、N-苄基-N-异丙基-N'-(4-三氟甲基)苯尿素、亚牛磺酸、D-葡萄糖-6-磷酸、顺-2-癸烯酸、二十碳二烯酸(顺-11,14)、前列腺素A2和10-羟基癸烯酸,显著下调(P<0.01)的代谢物为溶血磷脂酰胆碱22∶5、溶血磷脂酸22∶6和溶血磷脂酸22∶5。差异代谢物主要富集在丙氨酸、天冬氨酸和谷氨酸代谢通路(MapID:map00250),以及牛磺酸和亚牛磺酸代谢通路(MapID:map00430)。结论广藿香能显著影响贵州小型猪的溶血磷脂酸类物质的代谢,可能通过影响天冬氨酸-谷氨酸代谢通路、牛磺酸-亚牛磺酸代谢通路,起到缓解机体氨基酸代谢紊乱、调节炎性反应发生等功能。
