A COMPARATIVE STUDY ON THE TREATMENT OF VASCULAR DEMENTIA BY PUNCTURING BAIHUI (GV20), SHUIGOU (GV26) AND SHENMEN (HT7)

Objective: To observe the relative specialty of the therapeutic effect of acupuncture of Baihui (百会 GV 20), Shuigou (水沟 GV 26) a nd Shenmen (神门 HT 7) in the treatment of vascular dementia (VD) patients. Methods: F ifty VD patients were randomly divided into routine treatment group (control gro up), Baihui (GV 20) group (GV 20 group), Shuigou (GV 26) group (GV 26 gr oup), Shenmen (HT 7) group (HT 7 group) a nd GV 20+GV 26+HT 7 group (joint treatment group), with 10 cases in ea ch group. In control group, acupoints used were Jianyu (肩NFDA1 LI 15), Quchi (曲池 L I 11), Waiguan (外关 TE 5), etc. (which were also used in the other four groups) on the paralyzed side. The treatment was conducted once a day except weekends, 20 session s all together. Clinical Dementia Rating Scale (CDR), Mini-Mental State Examin at ion (MMSE) test, Blessed Dementia Rating Scale (BDR) and Clinical Neurological D eficit Rating (CNDR) were used to assess the patients’ intelligence state befor e and after acupuncture treatment. Results: After acupuncture tr eatment, the VD patients’ intelligence in all the five groups was improved at different degrees . Additional acupuncture of each of GV 20, GV 26 and HT 7 had a remarkable e ffec t in improving the VD patients’ fluid intelligence, and could obviously promote their abilities of temporal orientation, spatial orientation and figure drawing ; and additional joint acupuncture of the three acupoints could improve their co gnitive and non-cognitive functions, such as reducing the severity of dementia , raising the temporal orientation, spatial orientation, calculation, short-term m emory and figure drawing abilities, and improving their activities of daily livi ng and personality. Besides, acupuncture of GV 20 could improve the abilities of calculation and short term memory, and correct the personality change of VD pat ients, while acupuncture of GV 26 could improve the abilities of naming and sh or t-term memory. Conclusion: Acupuncture of GV 20, GV 26 and HT 7 all had a certai n therapeutic effect in improving VD; the therapeutic effects of acupuncture of GV 20 and GV 26 were better than that of acupuncture of HT 7; and the effica cy of the three points used in combination was the best.

Functional Magnetic Resonance Imaging Study on Acupuncturing Shenmen (HT 7) and Sham Acupoint

Objective： To observe the conditions of the activating areas in the brain evoked by needling Shenmen （HT 7） acupoint. Methods： Twelve healthy young volunteers were needled at Shenmen （HT 7） acupoint with rotating and twisting techniques. Functional magnetic resonance imaging （fMRI） was adopted to detect the brain activating areas, TR/TE/FA=3560 ms/50 ms/90°. After scanning, the images were dealt with SPM2. Results： Needling Shenmen （HT 7） primarily activated BA2 and BA1 of the gyrus postcentralis in right-sided frontal lobe, BA47 of left-sided inferior frontal gyrus and BA43 of left-sided gyrus postcentralis, and also activated BA40 of inferior parietal lobule in right-sided parietal lobe, BA22 of gyrus temporalis superior in left-sided lobus temporalis, and BA40 of right-sided insula; however, the sham acupoints could not activate these nucleus. Conclusion： Needling Shenmen （HT 7） can activate the relevant functional areas in the brain.

经皮自控电刺激耳神门穴对乳腺癌术后疼痛及恶心、呕吐的影响

目的观察经皮自控电刺激耳神门穴对乳腺癌术后疼痛及恶心、呕吐发生率的影响。方法选择2022年10月—2023年3月拟实施择期乳腺癌手术的患者80例。采用随机数字表分为耳神门穴刺激组(transcutaneous auricular Shenmen stimulation,ta-SMS组)和对照组(C组),每组40例。ta-SMS组入手术室后在预麻间行耳神门穴电刺激30 min、进入麻醉恢复室后即刻及回到病房后每隔3 h自控行经皮耳神门穴电刺激30 min。C组除不予耳穴电刺激外,其余处理同ta-SMS组。记录两组患者入室时心率(heart rate,HR)、平均无创血压(mean blood pressure,MBP)、脑电双频指数(bispectral index,BIS)及术后24、48、72 h的恶心、呕吐(post-operative nausea and vomiting,PONV)发生率;比较两组患者术后视觉模拟评分法(visual analogue scale,VAS)疼痛评分变化、镇痛泵按压总次数以及手术前后血清5-羟色胺(5-HT)表达水平。结果ta-SMS组患者较C组术后72 h内恶心、呕吐发生率显著降低(P<0.05);两组术后24、48、72 h的VAS评分差异无统计学意义(P>0.05);ta-SMS组PCIA按压总次数、镇痛复合液总消耗量降低,差异有统计学意义(P<0.05);两组术前血清5-HT水平差异无统计学意义(P>0.05);ta-SMS组术后24、48、72 h血清5-HT水平显著低于C组,差异有统计学意义(P<0.05)。结论多次经皮自控电刺激耳神门穴可降低乳腺癌手术后72 h内恶心、呕吐的发生率,缓解患者疼痛;5-HT的表达下调参与了其过程。

Comparative Study on the Effect of Baihui （GV 20）, Shuigou （GV 26） and Shenmen （HT 7） on Cognition of Patients with Vascular Dementia

Objective： To study the relative specificity of Baihui （GV 20）, Shuigou （GV 26） and Shenmen （HT 7） in improving the cognitive function of patients with vascular dementia （VD）. Method： Fifty VD cases were randomized into 5 groups, which were treated with routine points, routine points plus Baihui （GV 20）, routine points plus Shuigou （GV 26）, routine points plus Shenmen （HT 7）, and routine points plus the above-mentioned three points together respectively. The scale of elderly cognitive function （SECF） was measured before and after the treatment for statistical management. Results： The total score of SECF was markedly increased after Baihui （GV 20）, Shuigou （GV 26） and Shenmen （HT 7） were added to the routine points, more specifically, with addition of Baihui （GV 20）, the patients with VD got increased scores in such aspects as orientation, instant memory, long-term anamnesis, animal names, cancellation and calculation, and classification and analogy; with addition of Shuigou （GV 26）, the patients with VD got increased scores in such aspects as orientation, cancellation and calculation, and classification and analogy; with addition of Shenmen （HT 7）, the patients with VD got increased scores in such aspects as digital scope, animal names, and classification and analogy. Conclusion： Baihui （GV 20）, Shuigou （GV 26） and Shenmen （HT 7） could improve the cognitive function of patients with VD, and the combination of the three points got the best result despite their respective specificity.

电针内关和神门穴对脑功能成像不同影响的观察

目的 :观察针刺内关和神门对脑功能成像的影响作用。方法 :采用功能磁共振成像技术研究电针内关和神门对不同脑区的激活状态。结果 :电针刺激内关主要引起额叶的激活 ,刺激神门主要引起颞叶的激活。结论 :针刺刺激不同的穴位 ,可以激活不同的脑区 ,电针内关和神门穴激活脑区的功能与智能有关 ,提示应用内关、神门穴治疗智能障碍有一定的客观性。

电针内关、神门穴对海洛因依赖者稽延性戒断症状疗效观察

目的观察和分析针刺不同穴位对海洛因依赖者稽延性戒断症状的干预效果,为科研、临床筛选针刺治疗海洛因依赖稽延性症状的特异穴位提供临床依据。方法将60例男性海洛因依赖者随机分为对照组、针刺1组、针刺2组,采用稽延性戒断症状评分量表和汉密尔顿焦虑量表观察各组患者治疗前、治疗过程中及治疗3星期后的变化。结果针刺1组、针刺2组在控制稽延性戒断症状上与对照组相比有统计学差异(P<0.01,P<0.05);但针刺1组、针刺2组之间比较差异没有统计学意义(P>0.05)。结论 针刺可有效缓解海洛因依赖者稽延期戒断症状和精神焦虑症状;治疗海洛因依赖者稽延期诸症时,内关穴与神门穴具有同样显著疗效,并且神门穴对于心神的异常具有更快更好的显效趋势。

针刺神门穴和养老穴激活不同脑功能区的功能性磁共振成像研究

目的:通过功能性磁共振成像(fMRI)来了解针刺神门穴和养老穴的脑激活区情况。方法:选取健康青年志愿者12例,采用组块模式(静息阶段与刺激阶段交替出现,重复3次,分为3个组块),手法捻转行针针刺右侧的神门穴或养老穴(捻转角度180°,频率1.5Hz)。评价针刺的感觉,使用SPM2对扫描后图像进行后处理以观察激活图、激活区的差别,并观察安全指标。结果:针刺捻转时,操作者指下均有得气感,所有受试者也均有"酸、麻、重、胀"的得气感。在校正像情况下(组分析,FWE,0.05,K≥10),神门穴主要激活了右侧额叶的中央后回BA2、BA1、BA43,左侧额下回BA47,其次为右侧顶叶的顶下小叶BA40、右侧额叶的额下回BA44、左侧颞叶的颞上回BA22、右侧脑岛BA40;养老穴主要激活了左顶叶的顶下小叶BA40,右侧额叶的额下回BA45、BA46,其次为左侧颞中回BA37、颞下回BA37、额上回BA10。结论:针刺神门穴和养老穴的激活脑区是不同的,可能与本经相关的经脉、脏腑联系密切相关。

电针“神门”、“太溪”穴对急性心肌缺血家兔的效应比较

目的:探讨电针"神门"、"太溪"穴对急性心肌缺血家兔心功能的影响,比较同名经原穴间的效应差异。方法:健康青紫蓝家兔40只,随机选择8只健康家兔作为正常对照组,余家兔经股静脉一次性注射垂体后叶素3U/kg进行模型复制。将模型复制成功的家兔随机分为模型对照组、电针"神门"组、电针"太溪"组和电针非经穴组,每组8只。观察电针前后各组家兔心功能变化情况。结果:电针"神门"组家兔的+dp/dt max、-dp/dt max、LVSP与模型组、电针非经穴组及电针"太溪"组比较差异有统计学意义(P<0.05)。电针"太溪"穴及电针非经穴组的+dp/dt max、-dp/dt max及LVSP与正常组比较差异有显著性意义(P<0.05),与模型比较差异无统计学意义(P>0.05)。结论:手少阴心经的原穴"神门"对心脏功能的调节作用优于其同名经的原穴"太溪"。

电针对急性心肌缺血家兔心功能及心交感神经电活动的影响

目的:通过观察电针不同经穴对急性心肌缺血家兔心功能及心交感神经放电的影响,探讨原、络穴作用的相对特异性。方法:50只家兔随机分为正常对照组、模型对照组、电针神门组和电针支正组,采用静脉注射脑垂体后叶素复制急性心肌缺血模型。用Biopac生物信号采集系统记录神经电信号和心功能各指标;同时接入SKY-A4生物信号处理系统进行统计分析,记录电针"神门""支正"穴前后急性心肌缺血家兔心功能及心交感神经电活动的变化。结果:电针神门组、电针支正组在停针即刻心功能指标和心交感神经电活动与模型对照组比较均有统计学意义(P<0.05)。两组间比较,电针神门组各项指标改善优于电针支正组(P<0.05)。结论:电针"神门""支正"穴均可显著改善急性心肌缺血家兔的心功能并可提高心肌缺血状态下心交感神经的兴奋性,且电针"神门"穴的作用优于"支正"穴。

电针对心肌缺血细胞G蛋白信号通路的影响

目的:分析电针对缺血心肌细胞G蛋白信号通路的影响,揭示电针抗心肌缺血的分子作用途径。方法:健康SD雄性大鼠20只,随机分为正常组、模型组、电针正常“神门”组、电针模型“神门”组,每组5只。采用冠状动脉结扎法复制心肌缺血模型,然后电针“神门”穴,予以1.3 mA、2 Hz方波刺激,每日1次,3 d后动物麻醉状态下活体取材,用基因芯片技术筛选各组G蛋白及其相关基因。结果:筛选到表达G蛋白15个,通过cAMP或Ca2+途径影响下游基因表达,调节细胞转录和应答。电针“神门”特异性G蛋白2个,主要是G蛋白γ亚型通过cAMP影响下游蛋白激酶Aβ2调节细胞转录。结论:电针抗心肌缺血可以通过调节多种G蛋白影响缺血心肌细胞内的基因表达,从而发挥保护心肌的作用,以Gi和Gq最为重要;心经与心脏间具有相对特异的分子联系途径,可能主要是Gγ和PKAβ2途径。

“内关”“神门”“心俞”间协同作用与拮抗作用实验研究

目的 :探讨作用相似的腧穴间是否存在协同或拮抗作用。方法 :选用乌头碱诱发的家兔室性心律失常模型 ,以心律失常持续时间为指标 ,观察“内关”、“神门”、“心俞”单用及合用后的疗效差异。结果 :“内关”、“神门”、“心俞”单用有显著疗效 ,且作用相近 ,合用后疗效无显著提高或下降。结论 :作用相似且作用强度相近的腧穴间不易出现拮抗作用 。

针刺对睡眠剥夺大鼠学习记忆功能及前额叶皮质BDNF表达的影响

目的观察针刺对睡眠剥夺(SD)大鼠学习记忆功能及前额叶皮质BDNF表达的影响,为针刺疗法用于临床治疗SD提供理论依据。方法将60只大鼠随机分为对照组、模型组和针刺组,每组20只。模型组大鼠通过水上站立法建立SD大鼠模型,针刺组通过针刺神门穴进行干预治疗,对照组大鼠不做任何处理。分别于SD24 h、72 h后利用Morris水迷宫方法检测各组大鼠学习记忆功能,然后处死取脑,通过免疫组化方法检测大鼠前额叶皮质BDNF表达量的变化。结果针刺组和模型组SD24 h、72 h后体重、定位航行时间、空间搜索实验结果及前额叶皮质内BDNF表达量与对照组比较,差异均具有统计学意义(P<0.05)。针刺组SD24 h、72 h后定位航行时间和空间搜索实验结果与模型组比较,差异均具有统计学意义(P<0.05)。针刺组SD72 h后体重及前额叶皮质内BDNF表达量与模型组比较,差异具有统计学意义(P<0.05)。结论针刺可明显改善SD导致大鼠学习记忆功能下降,并可改善大鼠前额叶皮质因SD导致的BDNF表达下降。

百会、水沟、神门影响血管性痴呆患者认知功能的比较研究

目的:探讨百会、水沟、神门改善血管性痴呆(VD)患者认知功能的相对特异性。方法:50例VD患者,随机分为5组,分别施以针对偏瘫的常规穴位针刺,常规穴位加用百会穴,加用水沟穴,加用神门穴,加用百会、水沟和神门穴。治疗前后运用老年认知功能量表(SECF)进行测评,评分结果纳入统计学处理。结果:常规穴位加用百会、水沟、神门等穴位治疗后,SECF总分有显著提高;加用百会穴治疗后,VD患者的定向、瞬间记忆、长回忆、动物名、划销和计算、分类与类同等方面评分有明显提高;加用水沟穴治疗后,VD患者的定向、划销和计算、分类与类同等方面评分有明显提高;加用神门穴治疗后,VD患者的数字广度、动物名、分类与类同等方面评分有明显提高。结论:百会、水沟、神门能够改善VD患者认知功能,三穴虽然分别有各自的相对特异性,但联合运用效果最佳。

针刺安神六穴配合四关穴治疗偏头痛的临床研究

目的观察针刺安神六穴配合四关穴治疗偏头痛的临床疗效。方法将128例符合纳入标准的偏头痛患者随机分为治疗组和对照组,每组64例。治疗组采用针刺安神六穴配合四关穴治疗,对照组采用常规针刺治疗。观察并比较两组治疗前后及随访时疼痛数字评分法(NRS)评分、头痛发作次数、头痛持续时间、头痛程度、伴随症状、国际抑郁自评量表(SDS)评分及国际焦虑自评量表(SAS)评分的变化情况。结果两组治疗2、4星期后及随访时NRS评分及各项头痛症状及功能评分与同组治疗前比较,差异均具有统计学意义(P<0.01,P<0.05)。治疗组治疗4星期后及随访时NRS评分、头痛持续时间及头痛程度与对照组比较,差异均具有统计学意义(P<0.05,P<0.01)。治疗组治疗2、4星期后及随访时SDS评分与SAS评分与同组治疗前比较,差异均具有统计学意义(P<0.01)。对照组治疗4星期后SDS评分和SAS评分与同组治疗前比较,差异均具有统计学意义(P<0.01)。两组治疗2、4星期后及随访时SDS评分和SAS评分比较,差异均具有统计学意义(P<0.05,P<0.01)。治疗组随访时SDS评分和SAS评分与同组治疗2星期后比较,差异均具有统计学意义(P<0.01)。结论针刺安神六穴配合四关穴是一种治疗偏头痛的有效方法,能减轻患者头痛程度,改善焦虑和抑郁情况。

Electroacupuncture modulates the activity of the hippocampus-nucleus tractus solitarius-vagus nerve pathway to reduce myocardial ischemic injury

The hippocampus is involved in the regulation of the autonomic nervous system,together with the hypothalamus and brainstem nuclei,such as the paraventricular nucleus and nucleus tractus solitarius.The vagus nerve-nucleus tractus solitarius pathway has an important role in cardiovascular reflex regulation.Myocardial ischemia has been shown to cause changes in the autonomic nervous system,affecting the dynamic equilibrium of the sympathetic and vagal nerves.However,it remains poorly understood how the hippocampus communicates with brainstem nuclei to regulate the autonomic nervous system and alleviate myocardial ischemic tissue damage.A rat model of acute myocardial ischemia（AMI） was made by ligating the left anterior descending branch of the coronary artery.Three days before ischemia,the hippocampal CA1 region was damaged.Then,3 days after ischemia,electroacupuncture（EA） at Shenmen（HT7）-Tongli（HT5） was performed（continuous wave,1 m A,2 Hz,duration of 30 minutes）.Cluster analysis of firing patterns showed that one type of neuron was found in rats in the sham and AMI groups.Three types of neurons were observed in the AMI ＋ EA group.Six types of neurons were found in the AMI ＋ EA ＋ Lesion group.Correlation analysis showed that the frequency of vagus nerve discharge in each group was negatively correlated with heart rate（HR）（P 〈 0.05,r =-0.424）,and positively correlated with mean arterial pressure（MAP）（P 〈 0.05,r = 0.40987） and the rate-pressure product（RPP）（P 〈 0.05,r = 0.4252）.The total frequency of the nucleus tractus solitarius discharge in each group was positively correlated with vagus nerve discharge（P 〈 0.01,r = 0.7021）,but not with hemodynamic index（HR： P 〉 0.05,r =-0.03263; MAP： P 〉 0.05,r =-0.08993; RPP： P 〉 0.05,r =-0.03263）.Some neurons（Neuron C） were negatively correlated with vagus nerve discharge,HR,MAP and RPP in the AMI ＋ EA group（vagus nerve discharge： P 〈 0.05,r =-0.87749; HR： P 〈 0.01,r =-0.91902; MAP： P 〈 0.05,r =-0.85691; RPP： P 〈 0.01,r =-0.91902）.Some neurons（Neurons C,D and E） were positively correlated with vagus nerve discharge,HR,MAP and RPP in the AMI ＋ EA ＋ Lesion group（vagus nerve discharge： P 〈 0.01,r = 0.8905,P 〈 0.01,r = 0.9725,P 〈 0.01,r = 0.9054; HR： P 〈 0.01,r = 0.9347,P 〈 0.01,r = 0.9089,P 〈 0.05,r = 0.8247; MAP： P 〈 0.05,r = 0.8474,P 〈 0.01,r = 0.9691,P 〈 0.01,r = 0.9027; RPP： P 〈 0.05,r = 0.8637,P 〈 0.01,r = 0.9407,P 〈 0.01,r = 0.9027）.These findings show that the hippocampus-nucleus tractus solitarius-vagus nerve pathway is involved in the cardioprotective effect of EA at the heart meridian.Some interneurons in the nucleus tractus solitarius may play a particularly important role in the cardiomodulatory process.

Effects of acupuncture stimulation on brain activation induced by cue-elicited alcohol craving

Acupuncture has been shown to be effective on alcohol use disorder.However,the underlying mechanism remains poorly understood.To investigate the effects of Shenmen(HT7)acupoint on brain activation induced by cue-elicited alcohol craving,30 right-handed healthy light to moderate alcohol drinkers were recruited from the community.They were randomly assigned to undergo acupuncture either at HT7(experimental acupoint,n=15)or Jingqu(LU8,control acupoint,n=15)acupoints.This randomized controlled study was performed in Daegu Haany University and Daegu-Gyeongbuk Medical Innovation Foundation,Republic of Korea.Recruitment and data collection were conducted from December 2018 to May 2019.The results showed that after acupuncture at HT7 acupoint,the activation of orbitofrontal cortex and dorsolateral prefrontal cortex was greatly increased,while the activation of dorsolateral prefrontal cortex was obviously reduced,and subject's craving for alcohol was reduced when he/she seeing alcohol-related video clips involving various alcohols(beer,wine,or soju)or drinking scenarios.Acupuncture at HT7 more greatly reduced subject's alcohol cravings than acupuncture at LU8 acupoint.These findings suggest that acupuncture can improve the self-control of mild to moderate social drinkers through the activation of the orbitofrontal cortex and dorsolateral prefrontal cortex,thereby reducing the craving for alcohol.The study protocol was approved by the Institutional Review Board of Daegu Haany University Korean Medicine Hospital,Republic of Korea(approval No.DHUMC-D-18026-PRO-02)on November 30,2018.

“醒脑安神调补肝肾”针刺法治疗绝经期综合征选穴应用

"醒脑安神调补肝肾"针刺法主穴选择:小醒脑(内关、印堂、三阴交)、四神聪、神门、关元、肾俞、肝俞、太溪、足三里、太冲。辨证配穴:肝肾阴虚加阴谷、照海;脾肾阳虚加命门、腰阳关、脾俞;辨病配穴:失眠配申脉、照海;精神不振、烦躁不安配大陵;潮热盗汗配合谷、复溜;头晕耳鸣配百会、风池、听会;大便溏薄配天枢、阴陵泉;浮肿配水分等。失眠、潮热汗出、情绪改变是绝经期阶段最显著的症状。"醒脑安神调补肝肾"针刺法重在醒脑安神,调补肝肾,调节机体阴阳平衡。

Specific Effects of Needling Different Acupoints of Patients with Vascular Dementia

Objective： To observe the effects of needling Baihui （GV 20）, Shuigou （GV 26） and Shenmen （HT 7） on cerebral glucose metabolism in patients with vascular dementia （VD） by cerebral functional imaging technique. Methods： Twenty-five patients with VD were divided into 5 groups （Group A, B, C, D and E） randomly. Patients in the Group A were treated by needling routine acupoints for hemiplegia （Acupoints of the three yang meridians of hand and foot）, and besides the routine acupoints, patients in the Group B were treated by needling Baihui（GV 20）, the Group C by Shuigou（GV 26）, the Group D by Shenmen（HT 7）, and the Group E by Baihui（GV 20）, Shuigou（GV 26）, and Shenmen（HT 7）. All the patients were examined by Positron Emission Tomography （PET） to detect the cerebral glucose metabolism in the bilateral frontal lobes（orbital gyri）, parietal lobes, temporal lobes（hippocampus and hippocampal gyrus）, occipital lobes, thalamus, lentiform nucleus, caudate nuclei, cingulate gyrus and cerebellum before treatments and after treatments. Results： After needling the routine acupoints for hemiplegia, glucose metabolism increased in lentiform nucleus and temporal lobe; needling Baihui（GV 20）, increased in frontal lobe, temporal lobe and lentiform nucleus; needling Shuigou（GV 26）, increased in frontal lobe, thalamus and lentiform nucleus; needling Shenmen（HT 7）, increased in parietal lobe and lentiform nucleus; and needling these three acupoints, increased in frontal lobe, temporal lobe, thalamus and lentiform nucleus. Conclusion： Needling Baihui（GV 20）, Shuigou（GV 26） and Shenmen（HT 7） affect glucose metabolism in different functional regions of the brain, and Baihui（GV 20）, Shuigou（GV26） and Shenmen（HT 7） relate to different functional regions of the brain.

电针对失眠模型大鼠焦虑状态及交感-肾上腺髓质系统的影响

目的:探讨电针"神门""三阴交"治疗失眠症的作用机制。方法:30只SD大鼠随机分为空白组、模型组、电针组,每组10只,模型组、电针组采用限制性制动法建立失眠模型。造模后,空白组和模型组同步抓取固定但不进行治疗,电针组电针"神门""三阴交"治疗,每天1次,每次15min,治疗4d。治疗后检测各组大鼠昼夜活动量、高架十字迷宫试验进入开放臂百分数和开放臂停留时间百分数,采用酶联免疫法检测各组大鼠血浆去甲肾上腺素(noradrenaline,NE)、多巴胺(dopamine,DA)、肾上腺素(epinephrine,EPI)和丘脑、脑干中NE、DA含量。结果:模型组较空白组白昼活动量明显增多(P<0.05),夜晚活动量显著减少(P<0.05),高架十字迷宫试验进入开放臂百分数和开放臂停留时间百分数均较空白组明显减小(均P<0.05),模型组血浆NE、DA、EPI以及丘脑和脑干中NE、DA含量均较空白组明显升高(均P<0.05);电针组较模型组白昼活动量明显减少(P<0.05),夜晚活动量显著增多(P<0.05),电针组进入开放臂百分数和开放臂停留时间百分数较模型组明显增大(均P<0.05),血浆NE、DA、EPI以及丘脑和脑干中NE、DA含量均较模型组明显降低(均P<0.05)。结论:电针"神门""三阴交"通过抑制交感-肾上腺髓质系统的过度兴奋治疗失眠。

神门、大陵针刺效应特异性探讨

目的:通过对功能性核磁共振成像(fMRI)技术下针刺神门穴和大陵穴激活不同脑功能区的观察,探讨两穴效应的相对特异性。方法:纳入右利手健康志愿者10例,分别针刺受试者的右侧神门和大陵穴,在fMRI下采用组块刺激模式进行扫描,所获得数据通过Matlab软件SPM5模块分析,观察两穴的脑激活区。结果:针刺神门穴主要激活的brodmann分区为:左侧BA10、BA13、BA47、BA22;右侧BA40、BA44。针刺大陵穴主要激活的brodmann分区为:左侧BA46、BA47、BA22、BA10、BA45;右侧BA44、BA9、BA6、BA40。结论:针刺神门穴与大陵穴激活的脑功能区不完全相同,两穴共同激活区为语言、认知功能等相关区域;神门穴特异激活情绪控制等区域,大陵穴特异激活自主神经功能支配等区域,两穴针刺效应各具特异性。