Effect of acupuncture at Lieque(列缺LU 7) on vertebral-basilar artery hemodynamics in patients with cervical vertigo

Objective To explore the therapeutic efficacy and mechanism of acupuncture at Lieque （列缺 LU 7） in treatment of cervical vertigo （CV）. Methods Forty CV patients met the inclusion criteria were enrolled and treated with acupuncture at bilateral LU 7 points, once daily with 10 times as a course. The changes of blood flow velocity in bilateral vertebral artery and basilar artery were observed through transcranial doppler sonography （TCD） before treatment, after deqi with acupuncture and after a course of treatment, respectively. The scores and therapeutic efficacy on clinical symptoms were assessed according to CV symptoms and the functional assessment scale as well as the Criteria-for Diagnosis and Curative Effect in TCM Syndromes before treatment and after a course of treatment. Results Before treatment, after deqi and after a course of treatment, the blood flow velocity of basilar artery （BA）, left vertebral artery （LVA） and right vertebral artery （RVA） in 22 patients with a decreased blood flow velocity were respectively 23.20±4.84 vs 26.30 ± 4.17 vs 29.20 ± 4.20 （mm/s, BA）, 21.65 ± 3.62 vs 24.20 ± 2.89 vs 26.40 ± 3.62 （mm/s, LVA） and 21.90±3.04 vs 24.25±3.01 vs 26.50_±3.95 （mm/s, RVA）, while in 18 patients with an increased blood flow velocity were respectively 39.94 ± 8.24 vs 35.17 ± 4.84 vs 32.06 ± 3.49 （mm/s, BA）, 41.83 ± 5.64 vs 37.28 ± 2.32 vs 35.61 ± 2.09 （mm/s, LVA） and 37.11 ± 9.83 vs 32.22 ± 6.13 vs 28.11 ± 4.12 （mm/s, RVA）. Except that the difference of blood flow velocity of RVA was not significant in patients with an increased blood flow velocity before treatment and after deqi （P 〉 0.05）, the Vm of all vessels in 40 patients was improved after deqi with acupuncture and a course of treatment （P〈0.01, P〈0.05）. According to CV symptoms and the functional assessment scale, before treatment and after a course of treatment, the scores were 15.68 ± 5.35 and 26.30 ± 3.76, respectively, indicating that after a course of treatment, the symptoms were significantly improved （P 〈 0.01）. According to the Criteria for Diagnosis and Curative E.f]ect in TCM Syndromes, after a course of treatment, the total effective rate was 100% （40/40） and the cured rate was 55.0% （22/40）. Conclusion Acupuncture at LU 7 not only improves the blood supply of vertebral-basilar artery in CV patients, but also has a significant curative effect on improving the clinical symptoms.

针刺列缺、照海配合药物治疗慢性咽炎疗效观察

目的观察针刺列缺、照海配合药物治疗慢性咽炎的临床疗效。方法将60例慢性咽炎患者随机分为治疗组和对照组,每组30例。治疗组采用针刺列缺、照海穴配合口服清咽甘露丸治疗,对照组采用口服清咽甘露丸治疗。比较两组治疗前后证候积分及临床疗效。结果治疗组总有效率为96.7%,对照组为83.3%,两组比较差异具有统计学意义(P<0.05)。两组治疗后证候积分与同组治疗前比较,差异均具有统计学意义(P<0.05)。治疗组治疗后证候积分与对照组比较,差异具有统计学意义(P<0.05)。结论针刺列缺、照海配合口服清咽甘露丸是一种治疗慢性咽炎的有效方法。

列缺穴治疗落枕疗效观察

目的观察列缺穴单穴和配伍及落枕后患者就诊的时间对治疗效果的影响。方法将84例患者随机分为单穴组与配伍组,按患者就诊时间单穴组42例分为24 h内21例,24 h外21例;配伍组42例,分为24 h内21例与24 h外21例。单穴组运用一侧发病取对侧列缺穴,双侧发病取两侧列缺穴;配伍组列缺穴加风池、大杼、天柱、天窗、肩中俞。两组均每天针刺1次,每次留针30 min。对发病后24 h内和24 h外就诊患者进行治疗1次、2次、3次的疗效观察。结果 24 h内就诊患者两组治疗效果1次治愈率为单穴组95.2%,配伍组100%;24 h外就诊者1次治愈率为单穴组28.6%,配伍组38.1%;3次治愈率单穴组76.2%,配伍组95.2%。结论 列缺穴治疗落枕疗效与发病后就诊时间及配伍有关。

三维预处理技术初探

本文应用高阶近似LU分解法对三维椭圆型方程的正规七点差分矩阵给出了预处理阵,并对典型问题给出了预处理共轭梯度法的计算结果。文中所采用的分阶方论以及阶矩阵等概念,适用于一般的非奇异对角优势稀疏矩阵。对不同网格点数的计算结果表明,三维预处理共轭梯度法仍具有超线性的收敛速率,在高阶情形下超线性的特征尤为突出,由于非零对角线数增长过快,高阶的三维预处理方法不宜采用,零阶方法和一阶方法是值得推荐的。

基于数据挖掘分析针灸治疗桡骨茎突狭窄性腱鞘炎的选穴规律

目的:运用数据挖掘分析针灸治疗桡骨茎突狭窄性腱鞘炎的选穴共性和规律。方法:检索中国知网、万方数据库、维普中文期刊数据库和Pubmed数据库建库至2020年12月收录的针灸治疗桡骨茎突狭窄性腱鞘炎的临床研究文献,用Excel 2019软件建立针灸处方主穴数据库,采用数据挖掘技术中的关联规则方法,分析针灸处方经穴运用的特点和规律。结果:最终得到45篇符合纳入标准的文献,共纳入针灸处方45张。使用频次最高的穴位、经络和部位分别为阿是穴、手阳明大肠经和上肢腕关节。关联规则结果显示,置信度最高的穴位组合为手三里-列缺-阳溪,支持度最高的穴位组合是列缺-阳溪。聚类分析结果显示,存在2个有效聚类群,分别为“列缺-阳溪-合谷”“鱼际-温溜-太渊-外关-曲池-手三里”。结论:临床治疗桡骨茎突狭窄性腱鞘炎应以阿是穴为主穴,并结合具体情况配合使用列缺、阳溪、合谷等穴位进行施治。

肽能神经元对颈部急性炎性痛模型大鼠穴位敏化的调节作用

Objective:Pain is a health problem frequently reported in the clinical and general populations.Acupoint therapy is one of the most effective ways to treat pain;however,its mechanism remains unclear.Therefore,this study aimed to explore the relationship between distal acupoints,dorsal root ganglion(DRG)neurons,and spinal cord dorsal horn(SDH) neurons in neck acute inflammatory pain(NAIP).Methods:NAIP model rats were used to explore the relationship between acupoint sensitization and pain.Out of fourteen rats,ten rats were grouped into control and NAIP groups,five rats in control and five rats in NAIP.Mustard oil was subcutaneously injected on one side between the C4 and C7 vertebrae of the neck to establish an NAIP model.Evans blue(EB) was injected through the tail vein to detect sensitized acupoints after NAIP modeling.EB exudation in the body,Liè qūe(列缺 LU7),and Língdào(灵道 HT4) were evaluated.An immunofluorescence assay was conducted to detect the expression of calcitonin generelated peptide(CGRP),isolectin B4(IB4),and c-Fos in the dorsal root ganglion(DRG)and spinal cord dorsal medullae spinalis(CDMS).Four rats were used for the retrograde labeling of neurons of the LU7 region to the DRG and CDMS using CTB-488 and CTB-555 microinjection.Results:NAIP was shown to lead to oozing and pain sensitization in the LU7 and HT4 and increased the proportion of c-Fos^(+)/CGRP^(+)and c-Fos^(+)/IB4^(+)cells in both the DRG and CDMS.CTB-488 and CTB-555injected into the LU7 and sensitization point areas were observed in the DRG and CDMS regions of NAIP rats.Conclusions:Our study revealed that NAIP could lead to oozing and pain sensitization in the LU7 and HT4regions and that the pain point around LU7 might result from the transfer of peptidergic(CGRP-positive)and non-peptidergic(IB4-positive) neurons in the DRG and CDMS.

“头项寻列缺”的中医机制探讨

列缺穴位于前臂桡侧缘,为肺经络穴、八脉交会穴,具有沟通表里两经、舒筋活络止痛等作用。《四总穴歌》载"头项寻列缺",指出头项诸疾可通过针刺列缺穴治疗,并能取得良好的疗效,但对针刺列缺穴治疗头项诸疾的机理目前尚未明确。因此,笔者现从穴性、经脉循行、五运六气理论、经筋理论以及现代肌筋膜等角度对"头项寻列缺"的作用机理进行探析,为"头项寻列缺"的临床应用提供理论依据,进一步指导临床。

基于红外热像技术验证“头项寻列缺”理论

目的:验证列缺穴治疗头项部疾病是否通过表里同治这一作用途径实现。方法:选取甘肃中医药大学健康学生志愿者36例,采用随机数字表法随机分为列缺组和经渠组,每组18例。列缺组针刺受试者单侧列缺穴;经渠组采用与列缺同经且位置相近的经渠穴作为对照点针刺,使用红外热像技术测量两组针刺前后手太阴肺经上穴位(经渠、列缺、孔最、尺泽、天府)和手阳明大肠经上穴位(温溜、手三里、曲池、手五里、臂臑)的温度变化。结果:针刺列缺穴后,手太阴肺经上穴位(经渠、列缺、孔最、尺泽、天府)和手阳明大肠经上穴位(温溜、手三里、曲池、手五里、臂臑)的温度均明显高于针刺前(均P<0.05);针刺经渠穴后,手太阴肺经的穴位温度比针刺前明显上升(均P<0.05),手阳明大肠经的温度差异无统计学意义(均P>0.05)。结论:列缺穴治疗头项疾患可一穴通两经,起到表里同治的作用。

Clinical observation of Zhen'ai needling method in Nei Jing (Classic of Internal Medicine) on improving quality of life in patients with allergic rhinitis

Objective:To observe the effect of Zhen'ai needling method in Nei Jing (Classic of Internal Medicine) on quality of life in patients with allergic rhinitis (AR).Methods:A total of 99 patients with AR who met the inclusion criteria were randomized into a conventional needling group (51 cases) and a Zhen'ai needling group (48 cases).The conventional needling group was treated with acupuncture at Zusanli (ST 36),Hegu (LI 4),Juliao (ST 3),Yingxiang (LI 20),Yintang (GV 29),Shangxing (GV 23) and Baihui (GV 20).The Zhen'ai needling group added acupoints of Zhen'ai needling method {Lieque (LU 7),Shanglianquan [Extra,located at 1 cun above Lianquan (CV 23)] and Tianrong (SI 17)} in addition to points in the conventional needling group.The needles were retained for 30 min.The treatment was performed twice a week.All patients were assessed by total nasal symptom score (TNSS),total ocular symptom score (TOSS) and sino-nasal outcome test-20 (SNOT-20) before and after 15 treatments to evaluate the quality of life.The clinical efficacy was evaluated after 15 treatments.Results:During the treatment,8 cases dropped out in the conventional needling group and 7 cases in the Zhen'ai needling group.There were no significant differences in TNSS,TOSS and SNOT-20 scores between the two groups before treatment (all P＞0.05).After treatment,the total effective rate of the Zhen'ai needling group was 92.7％,versus 88.4％ in the conventional needling group.There was no significant difference between the two groups (P＞0.05).After treatment,there were no significant differences between the two groups in TNSS and TOSS scores (both P＞0.05).The SNOT-20 score of the Zhen'ai needling group was lower than that of the conventional needling group,and the difference was statistically significant (P＜0.05).There were statistical differences between the two groups in the scores of cough and nasal reflux (to the throat) of the NOT-20 scale (both P＜0.05).Conclusion:Both Zhen'ai needling method and conventional acupuncture are effective in the treatment of AR.Zhen'ai needling method has a better effect than conventional acupuncture in improving cough and nasal reflux (to the throat) in AR patients.

Clinical observation of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis accompanied by adenoid hypertrophy

Objective:To observe the clinical efficacy of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis(AR)accompanied by adenoid hypertrophy(AH).Methods:A total of 74 children who met the screening criteria were divided into a Zhen'ai group and a control group by the random number table method,with 37 cases in each group.The control group was treated with acupuncture at Zusanli(ST 36),Hegu(LI 4),Yingxiang(LI 20),Juliao(ST 3),Yintang(GV 29),Shangxing(GV 23)and Baihui(GV 20).The Zhen'ai group added points of Zhen'ai needling method{Shanglianquan[Extra,located at 1 cun above Lianquan(CV 23)],Tianrong(SI 17)and Lieque(LU 7)}in addition to the points in the control group.The needles were retained for 30 min.The treatment was performed twice a week.The total nasal symptom score(TNSS),sino-nasal outcome test-20(SNOT-20)and symptom scale for AH(SSAH)were assessed before and after 10 treatments.The clinical efficacy of the two groups was compared after treatment.Results:During the treatment,5 cases dropped out in the control group and 2 cases in the Zhen'ai group.After treatment,the total effective rate of the Zhen'ai group was 94.3%,versus 93.8%in the control group.There was no significant difference between the two groups(P>0.05).The markedly effective rate of the Zhen'ai group was 42.9%,versus 12.5%in the control group,and the difference was statistically significant(P<0.05).After treatment,there were significant intra-group differences in the scores of TNSS,SNOT-20 and SSAH in both groups(all P<0.05);the scores of SNOT-20 and SSAH in the Zhen'ai group were lower than those in the control group,and the differences between the groups were statistically significant(both P<0.05).Conclusion:Both conventional acupuncture and conventional acupuncture plus Zhen'ai needling method can improve clinical symptoms of children with AR accompanied by AH;and conventional acupuncture plus Zhen'ai needling method has a better effect than conventional acupuncture in improving AH symptoms.

Coronary arteriography under acupuncture anesthesia:a case report

Acupuncture anesthesia is a technique by partially or completely replacing anesthetics with acupuncture in surgery based on the traditional acupuncture analgesia mechanism.It emerged in the 1950s,went viral in the 1970s and then gradually fell into decline.In the recent years,this technique has regained attention and further research.Acupuncture anesthesia can be classified as either pure acupuncture anesthesia or acupuncture-medication combined anesthesia.To expand the application of this technique,a patient with non-ST elevation acute coronary syndrome in urgent need of percutaneous coronary intervention (PCI) received pure acupuncture anesthesia because of an allergy to lidocaine,and the operation went successfully.This is the first time that pure acupuncture anesthesia and coronary arteriography were combined,which is of great significance in further study and development of acupuncture anesthesia.

Protein and RNA expression of acid-sensing ion channels 2 and 3 in myocardial ischemia rats induced by isoproterenol

OBJECTIVE:To investigate the impact of electro-acupuncture at the Neiguan(PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2(ASIC2) and ASIC3 in myocardial ischemia rats.METHODS:Fifty male Sprague-Dawley rats were used,weighing(230 ± 50) g.The rats were randomized into a normal group A,model group B,Neiguan(PC 6) group C,Lieque(LU 7) group D,and A-shi points group E.There were 10 rats in each group.Rats were continuously administered 85 mg/kg intravenous isoproterenol daily to establish the model.Successfully modeled rats in groups C,D,and E were given electro-acupuncture treatment.Each group of rats was sacrificed with chloral hydrate(1 mL/100 g) intraperitoneal injection.The left ventricular myocardium was extracted and placed at- 70 ℃ until use.Western blot analysis and real-time PCR were performed to assay protein and RNA expressions of ASIC2 and ASIC3,respectively.Fold changes in RNA expression were quantified with the 2~^(-△△Ct) method.Blood samples were drawn from the aorta abdominalis and tested for creatine kinase-MB(CK-MB) and lactate dehydrogenase(LDH) levels using enzyme-linked immunosorbent assay.RESULTS:Myocardial ischemia rats given electro-acupuncture at the Neiguan(PC 6) acupoint had significantly lower protein and RNA expression of ASIC2 and ASIC3,and CK-MB and LDH levels,compared with model rats(P < 0.01).CONCLUSION:Electro-acupuncture at the Neiguan(PC 6) acupoint can not only decrease the protein and RNA expression of ASIC2 and ASIC3,but also inhibit the opening of ASICs and reduce the cardiomyocyte damage in myocardial ischemia rats.