Study on the regulatory effect of herbal cake-partitioned moxibustion on colonic CD206,AMPK and TSC2 in rats with Crohn disease

Objective:To explore the mechanism of herbal cake-partitioned moxibustion in Crohn disease(CD)treatment by observing the effect of herbal cake-partitioned moxibustion on protein expressions of colonic M2 macrophage marker CD206,AMP-activated protein kinase(AMPK)and tuberous sclerosis complex(TSC)2.Methods:Twenty-six specific pathogen free male rats were randomly divided into a normal group,a model group and a herbal cake-partitioned moxibustion group.The CD model was prepared by enema with the mixture of 5%(W/V)2,4,6-trinitrobenzene sulfonic acid(TNBS)and 50%ethanol at 2:1(volume ratio).After the model was successfully prepared,rats in the herbal cake-partitioned moxibustion group received herbal cake-partitioned moxibustion at Qihai(CV 6)and bilateral Tianshu(ST 25).Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of rat colon;immunohistochemical technique was used to detect the expression of colonic CD206 protein;Western blot,immuno fluorescence,and real-time fluorescence quantitative polymerase chain reaction(RTqPCR)technologies were used to detect the protein and mRNA expressions of colonic AMPK and TSC2.Results:Compared with the normal group,rats in the model group showed damaged colonic mucosa,missing of the epithelial layer;thickened submucosa,vascular proliferation,massive infiltration of monocytes and lymphocytes,and cracked ulcers that reached the muscle layer.Rats in the herbal cake-partitioned moxibustion group showed reduced intestinal inflammation and healing intestinal epithelium ulcers.Compared with the normal group,rat colonic CD206 protein expression,and the protein and mRNA expressions of colonic AMPK and TSC2 were decreased in the model group(all P<0.Ol);compared with the model group,rat colonic CD206 protei n expression was in creased(P<Q.Q1),as well as the protein and mRNA expressions of AMPK and TSC2 in the herbal cake-partitioned moxibustion(all P<0.05).Conclusion:Herbal cake-partitioned moxibustion can reduce intestinal inflammation in CD rats,increase colonic CD206 protein expression,and up-regulate the protein and mRNA expressions of colonic AMPK and TSC2.

曲骨穴古今文献应用特点分析

利用数据挖掘技术总结分析古今文献中曲骨的临床应用特点。以第5版《中华医典》为古代文献检索源,以中国期刊全文数据库(CNKI)、万方学术期刊全文数据库(Wanfang)、维普资讯中文期刊服务平台(VIP)为现代文献检索源,对曲骨单穴及配伍治疗病证、配伍腧穴、刺灸法等进行系统整理。纳入古代文献条文140条,曲骨单穴常见主治病证为癃闭、带下过多、疝气;配伍常见主治病证为带下过多、淋证、疝气;配伍腧穴64个,主要与任脉、膀胱经、肝经腧穴配伍,高频配穴为中极、关元、三阴交;配伍特定穴多使用五输穴;常用灸法。纳入现代文献73篇,曲骨单穴常见主治病证为尿潴留、勃起功能障碍、慢性前列腺炎;配伍常见主治病证为尿潴留、勃起功能障碍、前列腺增生;配伍腧穴36个,主要与任脉、肾经、脾经配伍腧穴,高频配穴为中极、关元、足三里;配伍特定穴多使用募穴;常用针刺疗法。古代曲骨主治病证广泛,重视腧穴的远治作用;现代曲骨治疗局部病证为主,重视腧穴的近治作用。

电针治疗良性前列腺增生及对患者雌雄激素水平的影响

目的:探讨电针对良性前列腺增生患者的疗效及对激素水平的影响。方法:将60例患者随机分为电针组和药物组,每组30例。电针组采用电针中极、曲骨穴治疗,每日1次,每周5次;药物组口服盐酸坦洛新缓释胶囊0.2 mg,每日1次,两组均治疗6周。观察两组治疗前后血清睾酮(T)、雌二醇(E_2)水平及雌雄激素比(E_2/T)的变化,采用国际标准进行前列腺症状评分(IPSS)及阴茎勃起功能评分(IIEF5),并检测血清前列腺特异抗原(PSA)及两组患者的不良反应情况,比较两组的临床疗效。结果:电针组治疗前后血清T、E_2、E_2/T比较差异均无统计学意义(均P>0.05),药物组治疗前后E_2/T比较差异有统计学意义(P<0.05);两组间血清T、E_2、E_2/T比较差异均无统计学意义(均P>0.05)。电针组IPSS评分较治疗前降低(P<0.05),药物组IPSS评分与治疗前比较差异无统计学意义(P>0.05);治疗后电针组IPSS评分低于药物组(P<0.05);电针组治疗后症状严重者明显减少,整体情况优于药物组(P<0.05)。两组IIEF5评分、PSA值治疗前后及组间比较差异均无统计学意义(均P>0.05)。电针组总有效率为60.7%(17/28),明显优于药物组的30.8%(8/26,P<0.05)。结论:电针中极、曲骨穴能明显减轻患者临床症状,疗效优于口服盐酸坦洛新缓释胶囊,对患者血清T、E_2水平未见明显影响。

Efficacy observation on acupuncture for diabetic gastroparesis

Objective： To observe the effect of acupuncture on gastric motility, plasma motilin and serum gastrin in patients with diabetic gastroparesis （DGP） and evaluate its clinical efficacy. Methods： A total of 100 eligible cases were randomly allocated into an acupuncture group （n=50） and a control group （n=50）. Patients in the acupuncture group were treated by needling Zhongwan （CV 12）, Zusanli （ST 36） and Neiguan （PC 6）, whereas patients in the control group were treated with oral administration of Domperidone. The clinical efficacies of the two groups were compared; and changes in gastric motility, plasma motilin and serum gastrin in both groups were observed before and after treatment. Results： After treatment, the symptom scores, gastric motility and contents of plasma motilin and serum gastrin were significantly improved in both groups （P〈0.05）. There were between-group statistically significant differences in symptom scores, gastric motility and levels of plasma motilin and serum gastrin after treatment （all P〈0.05）. The total effective rate was 96% in the treatment group, versus 78% in the control group, showing a statistically significant difference （P〈0.05）. Conclusion： Acupuncture is effective for DGP and can reduce the levels of plasma motilin and serum gastrin.