Effects of electroacupuncture at Guanyuan(CV 4)or Sanyinjiao(SP 6)on hypothalamus-pituitary-ovary axis and spatial learning and memory in female SAMP8 mice

OBJECTIVE: To investigate the effects of electroacupuncture(EA) at the Guanyuan(CV 4) or Sanyinjiao(SP 6) acupoints on the hypothalamus-pituitary-ovary(HPO) axis and spatial learning and memory in female mice.METHODS: Nine-month-old female mice with senescence-accelerated mouse prone 8(SAMP8)were divided into three groups: the disease model,EA-Guanyuan and EA-Sanyinjiao groups. Concurrently, 9-month old female mice with senescence-accelerated mouse resistance 1(SAMR1)were set as the control model group. The two treatment groups were given the same pattern of EA stimulation. Gonadotropin-releasing hormone, luteinizing hormone, follicle-stimulating hormone(FSH) and Serum estradiol levels in the Hypothalamus-pituitary-ovary axis were assessed by enzyme-linked immunosorbent assay to determinethe HPO axis function level. Spatial learning and memory were assessed by the Morris Water Maze(MWM) test.RESULTS:(a) HPO axis: compared with the control model group, the disease model group displayed a decrease in E2 levels(P < 0.01), and an increase in Gn RH, LH and FSH levels(P < 0.01). E2 levels were increased in EA treatment groups compared with the disease model group(P < 0.05). In contrast,Gn RH and LH and FSH levels were reduced(P <0.05). EA-Sanyinjiao group was superior than EA-Guanyuan group on increasing E2 and declining Gn RH levels(P < 0.01).(b) The MWM test demonstrated that the response latency in the EA-Sanyinjiao treatment group declined from day 2 to day5 compared with the disease model group(P <0.05), whereas the EA-Guanyuan treatment group showed no significant difference.CONCLUSION: EA can regulate hormone(E2, FSH,LH, Gn RH) levels in the HPO axis and the spatial learning and memory ability in female SAMP8 mice. Moreover, this effect may have been more pronounced in the EA-Sanyinjiao group than the EA-Guanyuan group. The underlying mechanism of the EA-induced changes may be related to gonadal hormone shifts in the HPO axis, followed by an improvement in spatial learning and memory.

Effect of lit-moxa stimulation of Guanyuan(CV 4) acupoint on levels of lactic acid and super-oxide dismutase in skeletal muscle of rats

OBJECTIVE:To observe the effect of lit-moxa stimulating acupoint Guanyuan(CV 4) on lactic acid and super-oxide dismutase(SOD) in skeletal muscle after exercise exhaustion.METHODS:Twenty-eight adult male Sprague-Dawley rats were randomly divided into normal control group,exhausted control group,exercise group and moxibustion group using exercise training and mild heating with lit-moxa stick as treatment methods.The exhausted control group,moxibustion group and exercise group received an exhaustive swimming after 20 days of intervention.Swimming exhausted times were recorded.Lactic acid and SOD concentration in soleus muscle were detected and compared between every two groups.RESULTS:The swimming exhausted times of the moxibustion group and the exercise group were significantly increased compare to the exhausted control group(P < 0.05).The lactic acid of the exhausted control group was significantly increased comparing with the normal control group(P <0.05),and the lactic acid of the moxibustion group and the exercise group were significantly lower than that of the exhausted control group(P < 0.05).The SOD level of the exhausted control group was significantly decreased comparing with the normal control group(P < 0.05),and the SOD level of the moxibustion group and the exercise group were significantly higher than that of the exhausted control group(both,P < 0.05).There was no statistical difference between the moxibustion group and the exercise group.CONCLUSION:With lit moxa stick,heat stimulating acupoint of Guanyuan(CV 4) decreased the levels of lactic acid and SOD in rat's skeletal muscle.

乳腺增生病不同证型关元穴红外辐射光谱探讨

目的:比较乳腺增生病患者不同证型任脉经穴关元穴体表红外辐射光谱特性。方法:采用高灵敏度PHE201体表红外光谱仪,观察了60例乳腺增生病患者不同证型关元穴1.5～16μm波段体表红外辐射光谱的差异。结果:乳腺增生病肝郁痰凝型患者与冲任失调型患者比较,两组关元穴体表红外辐射强度存在显著性差异。具有统计学意义差异的波段为:1.5～2.25μm,2.75μm,3μm,3.5μm,3.75～5μm,6～6.5μm,7μm,7.75μm,10.5～14μm,(P<0.05)。结论:关元穴红外辐射强度的差异可能与乳腺增生病不同证型有关。

针刺老年大鼠“足三里”“关元”穴对NO、SOD、MDA以及免疫影响的实验研究

目的 :探讨针刺抗衰老的作用机理。方法 :针刺老年大鼠的“足三里”“关元”穴 ,观察对脑、心、肝、肾组织中一氧化氮 (NO)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)变化以及对单核巨噬系统吞噬功能的影响。结果 :针刺能调节脑心肝肾组织中NO的含量 ;能提高脑心肝肾组织中抗氧化能力 ,使SOD的含量增高 ,MDA的含量降低 ;能提高单核巨噬系统的吞噬指数。结论 :针刺“足三里”“关元”

大椎、关元对腰椎间盘突出症临床疗效的影响

目的:探讨大椎、关元对腰椎间盘突出症临床疗效的影响,以及任督脉在腰椎间盘突出症临床治疗上的指导意义。方法:将427例腰椎间盘突出症患者随机单盲分为常规(循经取穴)组、常规加大椎组和常规加关元组进行治疗观察,采用u检验评定比较各组间疗效。结果:加大椎组和加关元组的治愈率与常规组比较,差异有显著或非常显著性意义(P<0.05,P<0.01),而有效率组间比较差异无显著性意义(P>0.05)。结论:大椎、关元可以提高腰椎间盘突出症的治愈率,调节任督、温补阳气可能是治疗腰椎间盘突出症的重要手段和方法之一。

经前隔姜灸治疗寒凝血瘀型原发性痛经疗效观察

目的观察经前隔姜灸神阙、关元穴治疗寒凝血瘀型原发性痛经的临床疗效。方法 65例寒凝血瘀型原发性痛经患者均在经前5 d采用隔姜灸神阙、关元穴治疗,共治疗3个月经周期。结果 65例患者经治疗后痊愈34例,显效23例,有效5例,总有效率为95.4%。65例患者治疗前痛经症状积分为(11.02±3.76)分,治疗后为(3.45±4.13)分,两者比较差异具有统计学意义(P<0.01)。结论经前隔姜灸是一种治疗寒凝血瘀型原发性痛经的有效方法。

艾箱灸用于无痛人工流产术后恢复的临床效果观察

目的:探讨艾箱灸在无痛人工流产术后恢复的临床疗效。方法:将180例早孕行无痛人工流产术者,随机分为治疗组和对照组各90例,进行前瞻性随机对照研究。治疗组术后30min及第1天分别予艾箱灸关元、中极穴30min;对照组不予艾箱灸,其余治疗方法相同。结果:治疗组术后1h和第1天宫缩痛的VAS(视觉模拟)评分,术后1h和第1天出血量,术后总出血量,出血持续时间,术后腹痛VAS评分和腹痛持续时间均低于对照组(P<0.01)。术后首次月经来潮时间及经期腹痛情况均优于对照组(P<0.01)。结论:无痛人工流产术后使用艾箱灸能明显减轻术后宫缩痛及腹痛的程度,缩短术后腹痛持续时间及出血时间,减少出血量,促进术后月经周期恢复,减轻经期腹痛,且安全无不良反应,值得临床推广应用。

针刺三阴交单穴及多穴对原发性痛经即刻镇痛效应临床观察

目的通过选用两组不同穴位对原发性痛经患者进行治疗,观察其对原发性痛经患者即刻镇痛效应的差异,为针刺治疗原发性痛经的优化治疗方案提供客观依据。方法将符合纳入标准的60例原发性痛经患者随机分为3组,其中A组(针刺三阴交)、B组(针刺三阴交、关元)、C组(空白组)各20例。观察本次月经周期针刺治疗前、针刺后及起针后3组的疼痛视觉模拟评分(Visual Analogue Scale,VAS)的变化情况。结果治疗后,A组及B组VAS评分与治疗前比较差异均有统计学意义(P<0.05);A组与B组VAS评分比较差异无统计学意义(P>0.05)。结论对于原发性痛经患者疼痛发作时,针刺三阴交及针刺三阴交、关元均有镇痛效应,但从优化方案角度考虑,单针三阴交穴即可。

Acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome from 2007 to 2016

Objective： To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome（PMS） from 2007 to 2016. Methods： Clinical literatures related to PMS treated with acupuncture-moxibustion published from 2007 to 2016 were collected from Chinese Biomedical Literature Database（CBM）, Chongqing VIP Database（CQVIP）, China National Knowledge Infrastructure（CNKI）, and Wanfang Academic Journal Full-text Database（Wanfang）. The retrieved data underwent descriptive analysis, cluster analysis and association pattern analysis to determine the acupoints selection principle in acupuncture-moxibustion treatment of PMS. Results： The top five acupoints used in acupuncture-moxibustion treatment of PMS were Sanyinjiao（SP 6）, Shenshu（BL 23）, Guanyuan（CV 4）, Baihui（GV 20）, and Shenmen（HT 7）. The leading 4 meridians were Bladder Meridian, Conception Vessel, Spleen Meridian, and Governor Vessel. The clustering analysis showed that the 5 core acupoint groups were：（1） Sanyinjiao（SP 6）;（2） Shenshu（BL 23） and Guanyuan（CV 4）;（3） Baihui（GV 20）, Shenmen（HT 7）, Zusanli（ST 36）, Ganshu（BL 18） and Taichong（LR 3）;（4） Taixi（KI 3）, Pishu（BL 20）, Xinshu（BL 15）, Qihai（CV 6） and Neiguan（PC 6）;（5） Sishencong（EX-HN 1）, Zhongwan（CV 12）, Hegu（LI 4）, Yintang（GV 29）, Fengchi（GB 20）, Zhongji（CV 3） and Feishu（BL 13）. The three most significant acupoints were Sanyinjiao（SP 6）, Shenshu（BL 23） and Guanyuan（CV 4）. Acupoint groups based on syndrome differentiation included：（1） Hegu（LI 4）, Zhongwan（CV 12） and Sishencong（EX-HN 1）;（2） Feishu（BL 13）, Zhongji（CV 3）, Fengchi（GB 20） and Yintang（GV 29）;（3） Xinshu（BL 15）, Pishu（BL 20）, Qihai（CV 6）, Neiguan（PC 6） and Taixi（KI 3）;（4） Ganshu（BL 18）, Zusanli（ST 36）, Shenmen（HT 7）, Taichong（LR 3） and Baihui（GV 20）. The analysis of association pattern elaborated that Shenshu（BL 23） and Sanyinjiao（SP 6） won the highest support rate in the paired groups; Ganshu（BL 18）, Shenshu（BL 23） and Sanyinjiao（SP 6） had the highest support rate among the acupoint groups. Conclusion： The data mining results of acupuncture-moxibustion treatment of PMS substantially conform to the general principle in traditional acupuncture-moxibustion theories, able to reflect the acupoints selection and grouping pattern and provide references for acupuncture-moxibustion treatment of PMS.

Assessment of Heat-sensitization at Guanyuan （CV4） in Patients with Primary Dysmenorrhea： A Comparative Study between Moxibustion Sensation and Infrared Thermography

Objective： To compare moxibustion sensation and infrared thermography by the assessment of heat-sensitization on Guanyuan （CV4） in patients with primary dysmenorrhea, and to prove the possibility of adopting infrared thermography as an objective demonstration of acupoints heat-sensitization. Methods： Seventy-one patients with primary dysmenorrhea were enrolled to receive moxibustion and infrared to detect the heat-sensitization of Guanyuan （CV 4）. The results were then analyzed and compared. Results： The infrared radiation showed a lower temperature when the acupoint Guanyuan （CV4） was sensitized in patients with primary dysmenorrhea. Compared with moxibustion sensation, its sensitivity rate （real positive rate） was 76.6%, divergence rate （real negative rate） was 70.1%, and the accuracy rate was 74.6%. After giving moxibustion to Guanyuan （CV4）, the infrared radiation area was significantly expanded longitudinally and transversely. Compared with moxibustion test, the sensitivity rate （real positive rate） of infrared test was 78.7%, divergencerate （real negative rate） was 83.3%, and the accuracy rate was 80.3%. Conclusion： To a certain extent, the heat-sensitization of the acupoint Guanyuan （CV4） in patients with primary dysmenorrhea can be revealed by infrared thermography. The acupoint heat-sensitization phenomena （heat extension or transmission） after giving moxibustion to the heat-sensitized acupoints can also be demonstrated objectively by infrared thermography, besides experienced subjectively by the receivers.

Effect of a comprehensive therapy plus Gushenyutai plaster administered at Guanyuan(CV 4) on male infertility associated with semen non-liquefaction

OBJECTIVE: To observe the clinical effect of Gushenyutai plaster administered at the Guanyuan(CV4) acupoint on male infertility associated with semen non-liquefaction.METHODS: Sixty-two male patients with infertility caused by semen non-liquefaction were random-ized into a treatment and control group. The control group received comprehensive therapy, which included oral administration of clarithromycin sustained release tablets, hip bath with Zhongyaoxiaoyan granules, prostate massage, and transurethral microwave treatment. The treatment group was administered Gushenyutai plaster in addition to the comprehensive therapy. Both groups were treated for 8 weeks. After treatment, the clinical effect and pregnancy rate were assessed and compared between the two groups.RESULTS: The effective rate of the treatment group was significantly higher than that of the control group(96.77% vs 70.97%, P<0.05) and the pregnancy rates of the treatment group and control groups were 38.71% and 16.13%, respectively(P<0.05).CONCLUSION: The effect of Gushenyutai plaster plus comprehensive therapy was better than that of the comprehensive therapy alone on male infertility induced by semen non-liquefaction.

基于“艾灸可治热证”理论探析湿热型溃疡性结肠炎的治疗

灸法可治热证,其基本理论支撑主要有火郁发之论、甘温除热论、热因热用论和双向调节论等。湿热型溃疡性结肠炎的病位在大肠,与肝、脾、肾三脏相关,与脾关系最为密切,其主要病机为脾虚湿盛,湿郁化热,证型中湿热为患居多。该病在血分,气血壅滞,病久及肺肾,本虚标实,脾肾亏虚为本,湿、热、痰、瘀、毒为标。以艾灸可治热证为理论基础,提出湿热型溃疡性结肠炎当以艾灸法温通经络,燥湿运脾,透发郁热,温助脾阳,以固后天之本,取关元、足三里进行艾灸,为中医治疗溃疡性结肠炎提供了新的思路与方法。

隔药饼灸对子宫内膜异位症大鼠过氧化物酶增殖体激活受体-γ和细胞间黏附分子-1表达的影响

目的:观察隔药饼灸对子宫内膜异位症大鼠过氧化物酶增殖体激活受体-γ(Peroxisome Proliferator-activated Receptor-γ,PPAR-γ)和细胞间黏附分子-1(ICAM-1)表达的影响。方法:选取34只大鼠,将其随机分为空白对照组(8只)和造模组(26只),采用改良的造模法建立子宫内膜异位症大鼠模型。将造模成功的24只大鼠随机分为模型组、隔药饼灸组和丹那唑组,每组8只。采用Western blot检测各组样本ICAM-1和PPAR-γ的表达。结果:与空白对照组比较,模型组、隔药饼灸组和丹那唑组大鼠的ICAM-1的表达显著上升(均P<0.05)。与模型组比较,隔药饼灸组和丹那唑组的ICAM-1的表达显著降低(均P<0.05);与空白对照组比较,模型组、隔药饼灸组和丹那唑组PPAR-γ的表达显著降低(均P<0.05),与模型组比较,隔药饼灸组和丹那唑组的PPAR-γ的表达显著增强(均P<0.05)。结论:隔药饼灸能抑制ICAM-1表达,促进PPAR-γ的表达,从而抑制子宫内膜细胞的黏附,阻止血管生成。

Anti-aging effects of moxa cone moxibustion As a free radical scavenger complement

Therapies that complement free radical scavenging are an important approach for treating aging in the brain. In the present study, two formulations of moxa cone moxibustion were applied at acupoints Zusanfi （ST 36） and Xuanzhong （GB 39）, and at acupoints Baihui （DU 20） and Guanyuan （RN 4）, in D-galactose-induced senile mice. The results revealed that moxa cone moxibustion improved total superoxide dismutase and Cu/Zn-superoxide dismutase activity in the homogenates of the cerebral tissue, as well as ameliorating deficits in neuronal morphology and neuronal density in the cerebral cortex and hippocampal CA3. Moxa cone moxibustion also enhanced learning and memory functions of senile mice. Moxa cone moxibustion at Zusanli, Xuanzhong, Baihuiand Guanyuan acupoints can thus be used to complement free radical scavengers, with efficacy that is equal to that of electroacupuncture at Zusanliand Xuanzhong, and superior to that of nimodipine treatment.

Clinical Application of Moxibustion on Guanyuan(CV 4)

Guanyuan （CV 4） is located 3 cun below the umbilicus, on the midline of abdomen. It is the Front-Mu Point of the Small Intestine, a crossing point of Three Yin Meridians of Foot and Conception Vessel. It can warm the kidney to invigorate yang, tonify the Yuan-Primordial qi, dredge and regulate Thoroughfare and Conception Vessels,

压灸百会、关元对轻中度阿尔茨海默病患者认知障碍及血清Aβ_(1-42)、tau、P-tau水平的影响

目的:比较压灸百会、关元联合盐酸多奈哌齐片与单纯盐酸多奈哌齐片治疗轻中度阿尔茨海默病(AD)患者认知障碍的临床疗效,从血清β淀粉样蛋白1-42(Aβ_(1-42))、微管相关蛋白tau、磷酸化tau(P-tau)水平探讨压灸治疗轻中度AD的机制。方法:将76例轻中度AD患者随机分为观察组(38例,脱落4例)和对照组(38例,脱落2例)。对照组予口服盐酸多奈哌齐片(每次5 mg,每天1次);观察组在对照组基础上予压灸百会、关元治疗,每次每穴施灸5壮,隔日1次,每周3次。两组均连续治疗8周。比较两组患者治疗前后及治疗结束后4、12周简易精神状态检查量表(MMSE)评分、蒙特利尔认知评估量表(MoCA)评分,治疗前后血清Aβ_(1-42)、tau、P-tau水平,并进行安全性评价。结果:治疗后各时间点,两组患者MMSE、MoCA评分均较治疗前升高(P<0.05),且观察组高于对照组(P<0.05)。治疗后,两组患者血清Aβ_(1-42)、tau、P-tau水平较治疗前降低(P<0.05),且观察组低于对照组(P<0.05)。两组患者安全等级比较差异无统计学意义(P>0.05)。结论:压灸百会、关元联合盐酸多奈哌齐片改善轻中度AD认知障碍的近远期疗效优于单纯盐酸多奈哌齐片,且可降低血清Aβ_(1-42)、tau、P-tau水平,这可能是压灸改善认知障碍的机制之一。

穴位埋线关元穴、肾俞穴对慢性前列腺炎大鼠IgG和血清Zn的影响

目的:探讨简易埋线关元穴和肾俞穴对琼脂所致慢性非细菌性前列腺炎大鼠的前列腺匀浆IgG和血清Zn的影响。方法:将40只雄性SD大鼠定时定量喂养1周后,随机分为假手术组、模型对照组、肾俞组、关元组,共4组,每组10只,除假手术组外,其余各组大鼠均予以注射琼脂溶液建立模型;假手术组于同样部位、以同样方法注入氯化钠溶液。造模24 h后每7天埋线1次,连续4周。28 d后取材,采用单项免疫扩散法检测各组大鼠前列腺匀浆中IgG水平;原子吸收分光光度法检测各组大鼠前列腺血清Zn含量。结果:与假手术组相比,模型对照组的大鼠小便浑浊、活动度低、精神状态差,前列腺组织有水肿充血、粘连和硬结,前列腺匀浆中IgG含量增高,血清Zn含量降低,差异有统计学意义(P<0.05);与模型对照组对比,肾俞组和关元组的大鼠活动度、饮水量增高,小便浑浊度降低,前列腺组织仅见软性结节、粘连减少,前列腺匀浆中的IgG含量均降低,Zn含量均升高,差异有统计学意义(P<0.05)。结论:简易埋线肾俞穴和关元穴可以改善大鼠小便浑浊度和前列腺组织粘连程度,可能与降低慢性前列腺炎大鼠匀浆IgG的含量、升高血清Zn的含量有关。

“双固一通”温针灸治疗膝骨性关节炎疗效观察及对血清炎性因子表达的影响

目的:探讨"双固一通"温针灸治疗膝骨性关节炎(KOA)的临床疗效及作用机制。方法:选取KOA患者90例,随机分为观察组和对照组各45例,对照组口服双氯芬酸钠缓释片治疗,观察组给予"双固一通"温针灸法治疗,观察并比较两组患者治疗前后膝关节功能、血清炎性因子水平,评定临床疗效。结果:治疗后观察组疼痛评分、关节僵硬评分、日常生活评分及总分均低于对照组(P<0.01);血清转化生长因子β(TGF-β)、胰岛素样生长因子-1(IGF-1)、成纤维细胞生长因子-2(FGF-2)水平均高于对照组(P<0.01),治疗愈显率高于对照组(P<0.05)。结论:"双固一通"温针灸法可有效改善KOA患者膝关节功能,抑制炎症反应,疗效显著。

A Systematic Review and Meta-Analysis of Herb-Partitioned Moxibustion in the Treatment of Primary Dysmenorrhea

OBJECTIVE: To systematically evaluate the clinical effects of herb-partitioned moxibustion on patients with primary dysmenorrhea. METHODS: Randomized controlled trials of herb-partitioned moxibustion for primary dysmenorrhea published from the year the database was established to November, 2017 were searched from CNKI,Wanfang Database, VIP database, CBM, PubMed, Embase, and Cochrane Library. Data extraction and quality evaluation were performed in papers meeting inclusion criteria. The outcomes measurements included clinical effect rate, visual analogue score(VAS), plasma PGF2α, adverse events, recurrence rate, and quality of life. The Rev Man 5.3 software was applied for meta-analysis. RESULTS:(1) A total of 16 studies were eligible and included in the meta-analysis involved1126 patients.(2) A statistically significant increasing in clinical effective rate was found in HMP group compared with control group [OR=3.49, 95%CI(2.25, 5.39), P<0.00001].(3) There was significant difference between two groups in visual analogue score [MD=–7.48, 95%CI(–9.93, –5.02), P<0.00001], which suggested the patients who received HPM had lower VAS scales than those who received no HPM.(4) There was significantly difference in serum PGF2α between the groups receiving HPM and the control group [MD=–4.61, 95%CI(–9.19, –0.03), P=0.05].(5) 2 trials reported followup duration, and all included studies did not mention the recurrence of PD during the follow-up stage, and all included studies did not mention the improvement of quality of life of PD during period of management and follow-up stage.CONCLUSIONS: Herb-partitioned moxibustion is beneficial for primary dysmenorrhea. However, the conclusion is not completely accurate with limitations of the current RCTs. The safety of herb-partitioned moxibustion remains to be defined by future studies. Attention should be paid to long-term effects of herb-partitioned moxibustion in clinical research. High-quality RCTs are required to confirm the effectiveness of herb-partitioned moxibustion for primary dysmenorrhea.

电针不同腧穴对心肌缺血模型大鼠细胞凋亡及心肌细胞miRNAs表达的影响

目的:观察电针不同组腧穴对心肌缺血大鼠细胞凋亡相关血清、微小核糖核酸(microRNA,miRNA)表达的影响,探讨其作用机制。方法:48只Wistar雄性大鼠随机分为正常组、模型组、内关电针组(内关组)及标本配穴电针组(配穴组),每组12只。模型组、内关组和配穴组大鼠以盐酸异丙肾上腺素(isoprenaline hydrochloride,ISO)2mg/(kg·d)背部皮下注射14d的方法制备心肌缺血模型;正常组注射等量0.9%NaCl溶液。造模后,内关组选取"内关",配穴组选取"关元""足三里""内关"分别进行电针治疗;正常组、模型组大鼠只抓取固定,每天1次,共21d。采用酶联免疫吸附试验(ELISA)检测各组大鼠血清肌酸激酶同工酶(creatine phosphate enzyme isozyme,CK-MB)、血管内皮细胞黏附分子-1(vascular cell adhesion molecule,VCAM-1)及内皮素1(ET-1)的含量;DNA断裂的原位末端标记法(TUNEL法)检测心肌细胞凋亡指数(apoptosisindex,AI);实时定量荧光PCR法检测心肌细胞miRNA-1、miRNA-133、miRNA-208、miRNA-499的表达量。结果:与正常组比较,模型组、内关组、配穴组大鼠血清CK-MB、VCAM-1、ET-1升高(均P<0.01),心肌细胞凋亡指数增多(均P<0.01);内关组、配穴组CK-MB、VCAM-1、ET-1均明显低于模型组(均P<0.01),心肌细胞凋亡指数下降(均P<0.01),且配穴组低于内关组(P<0.05)。与正常组比较,模型组心肌细胞miRNA-133表达降低(P<0.01),miRNA-208、miRNA-1、miRNA-499表达升高(均P<0.01);与模型组比较,内关组、配穴组心肌细胞miRNA-133表达增加(均P<0.01),miRNA-208、miRNA-1、miRNA-499表达降低(均P<0.01);与内关组比较,配穴组miRNA-133表达增加(P<0.01),miRNA-208、miRNA-1、miRNA-499表达降低(P<0.01,P<0.05)。结论:电针腧穴尤其是配穴组可有效防治心肌缺血,其保护机制可能与上调miRNA-133的表达,抑制心肌miRNA-1、miRNA-208、miRNA-499表达的双重调控作用有关。