梁丘穴联合足三里穴电针刺激时的fMRI研究

目的:利用f MRI观察足阳明胃经上的梁丘穴联合足三里穴电针刺激时大脑的活动规律。方法:选取25例体型中等、右利手的健康成年人,其中男13例,女12例;年龄22～50岁,选取受试者右侧梁丘穴联合右侧足三里穴进行电针刺激,同时行f MRI。结果:梁丘穴联合足三里穴电针刺激时,所有受试者均产生酸、麻、胀、钝痛、重和热的复杂针感,且强度不一。f MRI表现为以激活或负激活峰值为中心的不规则簇状区,且多个相近的激活或负激活脑功能区相连成片。其中脑功能激活区的峰值位于:(1)左侧尾状核体部和右侧豆状核;(2)左侧颞下回(BA20)、左侧海马旁回及左侧尾状核尾;(3)右侧海马旁回、右侧海马、右侧海马旁回(杏仁核包含在其中);(4)左侧海马旁回(BA30)、左侧后扣带回(BA29、30);(5)右侧中央前回(BA6)、右侧角回(BA39)。而脑功能负激活区的峰值位于:(1)小脑右半球前叶、小脑左半球前叶蚓部;(2)左侧颞上回(BA22)、左侧屏状核、左丘脑;(3)右侧中央后回(BA2)、右侧顶下小叶(BA40)、右侧中央前回(BA44);(4)左侧顶下小叶(BA40);(5)左侧中央前回(BA5、BA6);(6)右侧颞下回(BA37)。结论:梁丘穴联合足三里穴进行电针刺激时,激活脑区和负激活脑区较单独针刺足三里穴时明显集中、激活或负激活程度明显增高;大部分活动脑区与足三里穴单独针刺时相同;部分活动脑区与足三里穴单独针刺时不同。

温针灸治疗膝骨性关节炎疗效研究

目的:观察温针灸治疗膝骨性关节炎各期临床疗效。方法:将140例膝骨性关节炎患者随机分为对照组和观察组各70例。对照组采用常规西药治疗,观察组采用温针灸治疗,连续治疗3个疗程后,观察两组患者临床治疗疗效。结果:观察组治疗总有效率94.28%明显高于75.71%P<0.05);治疗后两组患者WOMAC骨关节炎指数明显降低,与治疗前对比(P<0.05),观察组降低明显优于对照组(P<0.05);治疗后两组患者疼痛评分明显降低,与治疗前对比(P<0.05),观察组降低明显优于对照组(P<0.05);两组患者临床症状评分明显降低,与治疗前对比(P<0.05),观察组降低明显优于对照组(P<0.05);治疗两组亚健康期、早期患者临床总有效率对比(P>0.05),观察组中期、晚期患者临床治疗总有效率与对照组对比(P>0.05),中期、晚期患者痊愈率、显效率明显高于对照组(P<0.05);治疗和观察期间两组患者均未发生严重不良反应(P>0.05)。结论:温针灸治疗膝骨性关节炎各期疗效确切,随着病情的严重,病期的深入,温针灸治疗更具有针对性,可显著减轻患者疼痛,改善临床症状,促进膝关节功能恢复。

Instant effect of moxibustion at different points on electrogastrogram of patients with functional dyspepsia

Objective： To observe different effects of moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） on electrogastrogram （EGG） of patients with functional dyspepsia （FD）. Methods： A total of 65 cases were randomized into a Zusanli （ST 36） group （n=33） and a Yanglingquan （GB 34） group （n=32） by random serial number, to receive moxibustion therapy to the corresponding point respectively. The 8-lead EGG was used before and after treatment to collect EGG parameter of the gastric body, antrum of stomach, lesser curvature and greater curvature, including the dominant frequency, slow wave percentage, average frequency, response area and motility index. Results： After treatment, the dominant frequency and slow wave percentage were improved in nearly the whole stomach area in Zusanli （ST 36） group, showing statistical significances （both P〈0.05）; the slow wave percentage and average frequency were improved in stomach body and antrum of stomach in Yanglingquan （GB 34） group, showing statistical significances （both P〈0.05）; moxibustion at Zusanli （ST 36） had a significantly higher influence on EGG than Yanglingquan （GB 34）, in which the lifting of slow wave percentage was significantly higher than Yanglingquan （GB 34） group （P〈0.05）. Conclusion： Moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） has the effect of adjusting abnormal EGG pattern in FD patients. Compared with Yanglingquan （GB 34）, Zusanli （ST 36） has a superior effect in improving EGG in stomach area.

Effect of electroacupuncture on JAK2/STAT3 pathway in synovial tissues of rats with rheumatoid arthritis

Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (RA) and to explore the action mechanism of EA on RA. Methods: Twelve of the 48 SPF male Sprague-Dawley (SD) rats were assigned to a normal group by the random number table method. The remaining 36 rats were subjected to RA model preparation by intradermal injection of the Freund's complete adjuvant into the right hind foot pad of each rat under sterile conditions. After the model was successfully prepared, rats were then divided into a model group, a drug group and an EA group according to a random number table method (n=12). Rats in the drug group were treated with 2 mL aqueous solution of tripterygium glycosides [8.1 mg/(kg?bw)];rats in the EA group were treated with EA at bilateral Yanglingquan (GB 34) and Zusanli (ST 36), for 30 min each time;rats in the normal group and the model group were placed in a special rat fixation tank for 30 min each time, and received the same dose of normal saline as those in the drug group. Rats in all groups received intervention once a day for 4 weeks. Diameter of rat ankle joint and rat arthritis index were measured before and after the intervention. At the end of the experiment, the expressions of phospho-JAK2 and phospho-STAT3 were determined by immunohistochemistry. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect JAK2 and STAT3 mRNAs expressions. Results: After the model was produced, the arthritis index >2 was considered successful in model preparation. Compared with the model group, the ankle joint diameters and arthritis indexes of rats in the drug group and the EA group were significantly lower (all P<0.01);immunohistochemical staining cells with phospho-JAK2 and phospho-STAT3 were significantly decreased (all P<0.01);the expression levels of JAK2 and STAT3 mRNAs were decreased with statistical differences (all P<0.01). There were no significant differences between the EA group and the drug group (all P>0.05). Conclusion: EA can alleviate the inflammatory response of RA rats, improve their pathological conditions, reduce the expressions of phospho-JAK2 and phospho-STAT3 in the synovial tissue of knee joint, and decrease the expressions of JAK2 and STAT3 mRNAs. The therapeutic effect of EA is comparable to that of the tripterygium glycosides. The mechanism of EA treatment may be related to the inactivation of the JAK2/STAT3 pathway.