莱阳市九个南瓜品种的果实品质比较

选取9个南瓜[Cucurbita moschata(Duchesne ex Lam.)Duchesne ex Poir.]品种进行果实品质及营养成分含量的比较,筛选出适宜山东省莱阳市种植的优质南瓜品种。结果表明,红板栗南瓜最轻,德丰银栗和红板栗南瓜的果实品质较好;甜蜜蜜红蜜南瓜的维生素C含量最高,红板栗南瓜次之;德丰银栗的可溶性糖含量最高。可见,德丰银栗和红板栗南瓜在物理性状和营养成分上表现的综合品质较优,是莱阳市值得推广和发展的优良品种。

微波辅助提取凤尾蕨中总黄酮的工艺研究

以黄酮提取率为考察指标,采用单因素试验和正交试验,进行了微波辅助乙醇提取凤尾蕨(Pteris multifide Poir)中总黄酮的研究,得到微波辅助提取凤尾蕨中总黄酮的最佳工艺条件为乙醇体积分数60%,料液比1∶60(g∶m L),微波功率320 W,微波提取时间60 s。此工艺条件下,凤尾蕨中总黄酮的提取率为3.318%。

New Chemical Compound from Caragana jubata(pall.) Poir.

A new pterocarpan glycoside, （-）-maackiain 3-O-6＇-O-acrylyl-β-D-galactopyranoside（1） was isolated from the whole parts of Caragana jubata（pall.） Poir., together with three known pterocarpan glycosides, （-）-maackiain 3-O-6＇-O-acetyl-β-D-glucopyranoside（2）, （-）-maackiain 3-O-β-D-glucopyranoside（3） and （-）-maackiain 3-O-β-D-galactopyranoside（4）. All the compounds were isolated from the title plant for the first time. The structure of the new compound was established on the basis of detailed 1D, 2D NMR and circular dichroism spectroscopic analyses. All the compounds were evaluated for their cytotoxic activities against three tumor cell lines, A549, HL-60 and P388.

Determination of Total Flavonoids in Leaves of Paliurus ramosissimus and Orthogonal Experiment Optimization Process

[Objectives] To screen the optimal extraction conditions of flavonoids from leaves of Paliurus ramosissimus( Lour.) Poir.,measure the content of flavonoids in leaves of P. ramosissimus. using the Microplate Reader,and provide experimental basis for the development of medicinal resources of P. ramosissimus. [Methods] The ultrasonic extraction method was adopted and orthogonal design was carried out to study the effects of ethanol concentration( A),ultrasonic time( B),the solid-to-liquid ratio( C) on the extraction of flavonoids from leaves of P. ramosissimus. The Microplate Reader was used to measure the content of flavonoids in leaves of P. ramosissimus. in different extraction conditions,P. ramosissimus. and the optimal extraction conditions were used to measure the content of flavonoids from leaves of P. ramosissimus.[Results]Effects of the ultrasonic extraction method on the extraction rate of flavonoids from leaves of P. ramosissimus: extraction times >ethanol concentration > Ultrasound time > solid-liquid ratio. The screened optimal extraction process was: ethanol concentration of 65%,the solid-to-liquid ratio of 1∶ 25,ultrasonic time of 2 h and extraction times Under this condition,the extraction rate of total flavonoids in leaves of P. ramosissimus. was 2. 56%. [Conclusions] This method is simple and feasibility,reliable,and suitable for rapid measurement with large samples.

气相色谱-质谱联用测定马甲子果脂溶性成分

用气相色谱-质谱联用技术鉴定马甲子果脂溶性成分,马甲子果用95%乙醇提取,浓缩液分别用石油醚、乙酸乙酯和正丁醇萃取,将石油醚层减压浓缩近干后用正己烷溶解,用气相色谱——质谱联用技术对其分析鉴定,以峰面积归一化法计算各组分的相对含量。结果检出51种化学组分,鉴定出其中28种,含量占总量的84.71%,其中甾类物质和酯类物质分别为65.13%和11.38%。甾体是马甲子果的主要成分。

Molecular Cloning and Expression of PoIR2,a Novel Gene Involved in Immune Response in Japanese Flounder (Paralichthys olivaceus)

A novel immune-related gene was expressed in Japanese flounder （Paralichthys olivaceus） injected with Vibrio anguillarum. The complete cDNA contained a 169 bp 5＇UTR, a 336 bp open reading frame （ORF） encoding 111 amino acids and a 556bp 3＇UTR. Six exons and five introns were identified in the PoIR2 gene. Blastp similarity comparison showed its encoding protein had 50% similarity to Danio rerio neuromedin S （NMS）, but further alignment indicated they did not have NMS C-terminal conservational signature domain. So it was not defined as an NMS homologue. Protein structure analysis indicated it had a 26aa sig- nal peptide and was a secretory pathway protein. RT-PCR demonstrated that the expression of PoIR2 was quickly induced and drastically increased in liver, kidney, spleen, gills, intestine, heart, and skeletal muscle after infected with V. anguillarurn. These results indicated that the PolR2 might play some important role in Japanese flounder immune response system. This gene was named PolR2 （P.olivaceus immune-related gene 2, GenBank accession number： EU224372）. The mature PoIR2 peptide was expressed in BL21 （DE3） pLysS using pET-32a（＋） vector and a great part of the recombinant mature peptide existed as soluble type.

Research on Materia Medica of Mongolian and Tibetan Medicinal Herb Caomaoxing

[Objectives] To investigate the basic source of the traditional Mongolian and Tibetan herb Caomaoxing. [Methods] The basic source of materia medica of Mongolian and Tibetan herb Caomaoxing was investigated through the collection of ancient books and modern literature about Mongolian and Tibetan medicine,combined with the current situation of clinical application. [Results] Through careful collection of literature and data,Caomaoxing recorded in Mongolian and Tibetan Meteria Medica is the red xylem heartwood of Caragana jubata( Pall.)Poir. [Conclusions] Through investigation of the basic source of Caomaoxing recorded in Mongolian and Tibetan Meteria Medica,this study is expected to provide literature reference for further research and development of Caomaoxing.

Digital imaging approaches for phenotyping whole plant nitrogen and phosphorus response in Brachypodium distachyon

This work evaluates the phenotypic response of the model grass （Brachypodium distacbyon （L.） P. Beauv.） to nitrogen and phosphorus nutrition using a combination of imaging techniques and destructive harvest of shoots and roots. Reference line Bd21-3 was grown in pots using 11 phosphorus and 11 nitrogen concentrations to establish a dose-response curve. Shoot biovolume and biomass, root length and biomass, and tissue phosphorus and nitrogen concentrations increased with nutrient concentration. Shoot biovolume, estimated by imaging, was highly correlated with dry weight （R2 〉 0.92） and both biovolume and growth rate responded strongly to nutrient availability. Higher nutrient supply increased nodal root length more than other root types. Photochemical efficiency was strongly reduced by low phosphorus concentrations as early as 1 week after germination, suggesting that this measurement may be suitable for high throughput screening of phosphorus response. In contrast, nitrogen concentration had little effect on photochemical efficiency. Changes in biovolume over time were used to compare growth rates of four accessions in response tonitrogen and phosphorus supply. We demonstrate that a time series image-based approach coupled with mathematical modeling provides higher resolution of genotypic response to nutrient supply than traditional destructive techniques and shows promise for high throughput screening and determina- tion of genomic regions associated with superior nutrient use efficiency.