[Objectives]This study was conducted to identify the pathogen species and dominant species of sugarcane pokkah boeng in main varieties in Yunnan sugarcane areas,so as to promote the healthy and sustainable development...[Objectives]This study was conducted to identify the pathogen species and dominant species of sugarcane pokkah boeng in main varieties in Yunnan sugarcane areas,so as to promote the healthy and sustainable development of sugarcane industry.[Methods]Specific primers Fv-F3/Fv-R3 and Fp-F4/Fp-R4 were designed based on the ribosomal DNA non-internal transcribed spacer(rDNA-ITS)gene sequences of Fusarium verticillioides and Fusarium proliferarum,the main pathogens of sugarcane pokkah boeng,and 117 typical sugarcane pokkah boeng samples collected from main varieties in different sugarcane areas of Yunnan Province were detected and analyzed by PCR.[Results]Among the 117 sugarcane pokkah boeng samples,112 samples were detected with F.verticillioides with a positive detection rate of 95.7%;103 samples were detected with F.proliferarum with a positive detection rate of 88%;103 samples were infected by F.verticillioides+F.proliferarum,and the compound infection rate was 88%;and the two pathogens were not detected in 5 samples,which might be sugarcane pokkah boeng caused by other species.PCR amplification products of 23 F.verticillioides positive samples and 19 F.proliferarum positive samples from different sugarcane varieties in different sugarcane areas were sequenced.The BLAST alignment results showed that the sequences of the 23 amplification products of F.verticillioides shared 99.45%-100.00%homology with F.verticillioides(GenBank accession number:KU508286),and the sequences of the 19 amplification products of F.proliferarum shared 99.26%-100.00%homology with the sequence of F.proliferarum(GenBank accession number:MK252904).Part of the F.verticillioids and 11 F.proliferarum sequences were selected to construct a phylogenetic tree,and the phylogenetic analysis showed that they belonged to the F.verticillioids group and F.proliferarum group,respectively.The results showed that F.verticillioides and F.proliferarum were the main pathogens causing sugarcane pokkah boeng of the main sugarcane varieties in Yunnan,and there was a common phenomenon of compound infection.F.verticillioides was the dominant species in Pu er,Lincang,Honghe and Yuxi sugarcane areas,but the detection rate of F.proliferarum was also high,and there were other species.In the future,the discovery of resistant germplasm resources and breeding of resistant varieties should be carried out aiming at these two pathogens of sugarcane pokkah boeng.[Conclusions]The study provides technical support for rapid identification of sugarcane pokkah boeng pathogens,and scientific basis for breeding resistant varieties and scientific disease prevention and control.展开更多
[Objectives]This study was conducted to determine the pathogen species and dominant species of sugarcane pokkah boeng in the sugarcane areas of Yunnan in the low-latitude plateau,so as to provide a basis for the patho...[Objectives]This study was conducted to determine the pathogen species and dominant species of sugarcane pokkah boeng in the sugarcane areas of Yunnan in the low-latitude plateau,so as to provide a basis for the pathogenic mechanism,disease resistance breeding and scientific prevention and control of pokkah boeng.[Methods]With 14 pokkah boeng samples collected from different sugarcane areas in Yunnan as materials,pokkah boeng-specific detection primers FvF4/Fv-R4 and Fp-F3/Fp-R3 were designed based on ribosomal DNA non-transcribed spacer(rDNA-ITS)sequences,respectively,and used to perform PCR on Fusarium verticillioides and F.proliferatum.[Results]In 12 of the 14 pokkah boeng samples,two species of pokkah boeng pathogens were detected,and there was a phenomenon of composite infection.Seven composite infection samples were selected for sequence alignment and phylogenetic analysis.The sequences of F.verticillioides(Gen Bank accession number:MZ126549-MZ126555)and F.proliferatum(Gen Bank accession number:MZ102259-MZ102265)from seven composite infection samples shared 98.6%-100%and 100%with F.verticillioides strain 20(Gen Bank accession number:KU508286)and F.proliferatum Dehong strain(GenBank accession number:KJ629482)published in Gen Bank,respectively.The phylogenetic tree showed that the pathogens of sugarcane pokkah boeng in Yunnan were mainly divided into the F.verticillioides group and F.proliferatum group.In the F.verticillioides group,except for ROC 25(Lancang,Yunnan)and Funong10-1405(Maitreya,Yunnan)which were on an independent branch,the remaining five composite infection samples were grouped with F.verticillioides strains from different geographical origins,and were closely related to F.oxysporum Guangxi strain.All strains of F.proliferatum from different geographic origins were clustered into another group.[Conclusions]This study provides a scientific basis for disease resistance breeding and disease prevention and control of sugarcane pokkah boeng.展开更多
基金Supported by China Agriculture Research System of MOF and MARA(CARS-170303)Yunling Industry and Technology Leading Talent Training Program(2018LJRC56)Special Fund for the Construction of Modern Agricultural Industry Technology System in Yunnan Province.
文摘[Objectives]This study was conducted to identify the pathogen species and dominant species of sugarcane pokkah boeng in main varieties in Yunnan sugarcane areas,so as to promote the healthy and sustainable development of sugarcane industry.[Methods]Specific primers Fv-F3/Fv-R3 and Fp-F4/Fp-R4 were designed based on the ribosomal DNA non-internal transcribed spacer(rDNA-ITS)gene sequences of Fusarium verticillioides and Fusarium proliferarum,the main pathogens of sugarcane pokkah boeng,and 117 typical sugarcane pokkah boeng samples collected from main varieties in different sugarcane areas of Yunnan Province were detected and analyzed by PCR.[Results]Among the 117 sugarcane pokkah boeng samples,112 samples were detected with F.verticillioides with a positive detection rate of 95.7%;103 samples were detected with F.proliferarum with a positive detection rate of 88%;103 samples were infected by F.verticillioides+F.proliferarum,and the compound infection rate was 88%;and the two pathogens were not detected in 5 samples,which might be sugarcane pokkah boeng caused by other species.PCR amplification products of 23 F.verticillioides positive samples and 19 F.proliferarum positive samples from different sugarcane varieties in different sugarcane areas were sequenced.The BLAST alignment results showed that the sequences of the 23 amplification products of F.verticillioides shared 99.45%-100.00%homology with F.verticillioides(GenBank accession number:KU508286),and the sequences of the 19 amplification products of F.proliferarum shared 99.26%-100.00%homology with the sequence of F.proliferarum(GenBank accession number:MK252904).Part of the F.verticillioids and 11 F.proliferarum sequences were selected to construct a phylogenetic tree,and the phylogenetic analysis showed that they belonged to the F.verticillioids group and F.proliferarum group,respectively.The results showed that F.verticillioides and F.proliferarum were the main pathogens causing sugarcane pokkah boeng of the main sugarcane varieties in Yunnan,and there was a common phenomenon of compound infection.F.verticillioides was the dominant species in Pu er,Lincang,Honghe and Yuxi sugarcane areas,but the detection rate of F.proliferarum was also high,and there were other species.In the future,the discovery of resistant germplasm resources and breeding of resistant varieties should be carried out aiming at these two pathogens of sugarcane pokkah boeng.[Conclusions]The study provides technical support for rapid identification of sugarcane pokkah boeng pathogens,and scientific basis for breeding resistant varieties and scientific disease prevention and control.
基金Supported by China Agriculture Research System of MOF and MARA(CARS-170303)“Yunling Industry and Technology Leading Talent”Training Program"Prevention and Control of Sugarcane Pests"(2018LJRC56)Special Fund for the Construction of Modern Agricultural Industry Technology System in Yunnan Province(YNGZTX-4-92)。
文摘[Objectives]This study was conducted to determine the pathogen species and dominant species of sugarcane pokkah boeng in the sugarcane areas of Yunnan in the low-latitude plateau,so as to provide a basis for the pathogenic mechanism,disease resistance breeding and scientific prevention and control of pokkah boeng.[Methods]With 14 pokkah boeng samples collected from different sugarcane areas in Yunnan as materials,pokkah boeng-specific detection primers FvF4/Fv-R4 and Fp-F3/Fp-R3 were designed based on ribosomal DNA non-transcribed spacer(rDNA-ITS)sequences,respectively,and used to perform PCR on Fusarium verticillioides and F.proliferatum.[Results]In 12 of the 14 pokkah boeng samples,two species of pokkah boeng pathogens were detected,and there was a phenomenon of composite infection.Seven composite infection samples were selected for sequence alignment and phylogenetic analysis.The sequences of F.verticillioides(Gen Bank accession number:MZ126549-MZ126555)and F.proliferatum(Gen Bank accession number:MZ102259-MZ102265)from seven composite infection samples shared 98.6%-100%and 100%with F.verticillioides strain 20(Gen Bank accession number:KU508286)and F.proliferatum Dehong strain(GenBank accession number:KJ629482)published in Gen Bank,respectively.The phylogenetic tree showed that the pathogens of sugarcane pokkah boeng in Yunnan were mainly divided into the F.verticillioides group and F.proliferatum group.In the F.verticillioides group,except for ROC 25(Lancang,Yunnan)and Funong10-1405(Maitreya,Yunnan)which were on an independent branch,the remaining five composite infection samples were grouped with F.verticillioides strains from different geographical origins,and were closely related to F.oxysporum Guangxi strain.All strains of F.proliferatum from different geographic origins were clustered into another group.[Conclusions]This study provides a scientific basis for disease resistance breeding and disease prevention and control of sugarcane pokkah boeng.
