A cDNA encoding melittin from a wasp (Polistes hebraeus) was amplified and cloned into the GST fusion expression vector pGEX-4T-2. The expressed protein appeared on the SDS-PAGE profiles with an about 29 kDa band. Wes...A cDNA encoding melittin from a wasp (Polistes hebraeus) was amplified and cloned into the GST fusion expression vector pGEX-4T-2. The expressed protein appeared on the SDS-PAGE profiles with an about 29 kDa band. Western blotting proved that the recombinant protein is the fusion protein of GST-PhM. The expression conditions of GST-PhM fusion protein for E. coli BL21 transformant were optimized. Thin layer scanning on the SDS-PAGE profiles showed that the expressed target protein had accumulated up to about 10%～12% of total protein of bacterial cells under the optimized expression condition. Purified and recovered recombinant melittin of Polistes hebraeus showed bioactivity in activating rabbit platelets to aggregate.展开更多
The wasps of the genus Polistes have been considered the key to understanding the evolution of social behavior in Hymenoptera. Several studies have shown that the development of organized insect societies was accompan...The wasps of the genus Polistes have been considered the key to understanding the evolution of social behavior in Hymenoptera. Several studies have shown that the development of organized insect societies was accompanied by the evolution of structures like exocrine glands, which became specialized to perform specific functions. This article investigates the ultrastructural and cytochemical features of the hypopharyngeal glands of Polistes versicolor. These glands have been studied in depth in social bees, where they occur only in nurses and produce the royal jelly. Our results＇ revealed that these glands basically did not vary among individuals or between sexes. They are constituted by spherical cells, each with a large nucleus and well-developed rough endoplasmic reticulum. Secretion vesicles are abundant, but lipid droplets were not observed, indicating that these glands may not have a role in pheromone synthesis. Acid phosphatase was detected in lysosomes, and also flee in the cytosol, but did not seem to be related with cell death. Thus, our results suggest that the hypopharyngeal glands ofP. versicolor may not have a specialized social role, but could produce digestive enzymes.展开更多
基金Item supported by national natural sciencefoundation of China(No.30271008)Foundation of school ofagriculture and biology of SJTU(AE150054)
文摘A cDNA encoding melittin from a wasp (Polistes hebraeus) was amplified and cloned into the GST fusion expression vector pGEX-4T-2. The expressed protein appeared on the SDS-PAGE profiles with an about 29 kDa band. Western blotting proved that the recombinant protein is the fusion protein of GST-PhM. The expression conditions of GST-PhM fusion protein for E. coli BL21 transformant were optimized. Thin layer scanning on the SDS-PAGE profiles showed that the expressed target protein had accumulated up to about 10%～12% of total protein of bacterial cells under the optimized expression condition. Purified and recovered recombinant melittin of Polistes hebraeus showed bioactivity in activating rabbit platelets to aggregate.
文摘The wasps of the genus Polistes have been considered the key to understanding the evolution of social behavior in Hymenoptera. Several studies have shown that the development of organized insect societies was accompanied by the evolution of structures like exocrine glands, which became specialized to perform specific functions. This article investigates the ultrastructural and cytochemical features of the hypopharyngeal glands of Polistes versicolor. These glands have been studied in depth in social bees, where they occur only in nurses and produce the royal jelly. Our results＇ revealed that these glands basically did not vary among individuals or between sexes. They are constituted by spherical cells, each with a large nucleus and well-developed rough endoplasmic reticulum. Secretion vesicles are abundant, but lipid droplets were not observed, indicating that these glands may not have a role in pheromone synthesis. Acid phosphatase was detected in lysosomes, and also flee in the cytosol, but did not seem to be related with cell death. Thus, our results suggest that the hypopharyngeal glands ofP. versicolor may not have a specialized social role, but could produce digestive enzymes.
