The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species （ROS） and phenylpropanoid pathway were inv...The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species （ROS） and phenylpropanoid pathway were investigated in this paper. In vitro test indicated that thiamine significantly inhibited mycelia growth and spore germination of T. roseum and A. alternata. Thiamine at 100 mmol L-1 effectively inhibited lesion development of muskmelon fruit inoculated with T. roseum or A. alternata, enhanced production rate of O2; and H2O2 content, activities of catalase （CAT）, ascorbate peroxidase （APX） and glutathione reductase （GR） in muskmelon fruit. Thiamine also affect phenylpropanoid pathway in muskmelon fruit by increasing the activities of phenylalanine ammonia lyase （PAL） and peroxidase （POD）, the content of total phenolic compounds, flavonoids and lignin. These results suggest that the effects of thiamine on pink and black spot rots in muskmelon fruits are associated with its direct fungitoxic against the pathogens and the modulation of O2- and H2O2 production, elimi- nating enzymes and phenylpropanoid pathway.展开更多
The green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 pla...The green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling has been verified according to its GFP-related fluorescence and Southern blotting analysis. The results provided direct and convincing facts in cytology and molecular biology for the pollen-tube pathway method, an efficient transformation technique used in plants.展开更多
β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of...β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.展开更多
基金supported by the National Natural Science Foundation of China(31401554)the Doctoral Initial Funding of Bohai University,China(bsqd201405)
文摘The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species （ROS） and phenylpropanoid pathway were investigated in this paper. In vitro test indicated that thiamine significantly inhibited mycelia growth and spore germination of T. roseum and A. alternata. Thiamine at 100 mmol L-1 effectively inhibited lesion development of muskmelon fruit inoculated with T. roseum or A. alternata, enhanced production rate of O2; and H2O2 content, activities of catalase （CAT）, ascorbate peroxidase （APX） and glutathione reductase （GR） in muskmelon fruit. Thiamine also affect phenylpropanoid pathway in muskmelon fruit by increasing the activities of phenylalanine ammonia lyase （PAL） and peroxidase （POD）, the content of total phenolic compounds, flavonoids and lignin. These results suggest that the effects of thiamine on pink and black spot rots in muskmelon fruits are associated with its direct fungitoxic against the pathogens and the modulation of O2- and H2O2 production, elimi- nating enzymes and phenylpropanoid pathway.
文摘The green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling has been verified according to its GFP-related fluorescence and Southern blotting analysis. The results provided direct and convincing facts in cytology and molecular biology for the pollen-tube pathway method, an efficient transformation technique used in plants.
文摘β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.
