The expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients

Objective:The coal-fired pollution in Xuanwei area has been considered to be local main reason for high incidence of female lung cancers.The aim of the study was to investigate the expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients, and to explore the relationship between the large number of coal-fired pollution PAHs materials and the high incidence of Xuanwei female lung cancers.Methods:We totally collected each 20 cases of Xuanwei female lung cancer patients, Xuanwei male lung cancer patients, non-Xuanwei female lung cancer patients and collected each 10 cases of Xuanwei, non-Xuanwei female patients with benign lung lesions.The cancer tissues, adjacent cancer tissues and normal lung tissues were collected in lung cancer patients and only the normal tissues were collected in benign lung lesion patients.There were total 80 cases and 200 tissues.Using immunofluorescence, we detected the expression of PAH-DNA adducts in each group.Image pro-plus 6.0 software was used to analyze the images and did part quantified analysis.SPSS 13.0 statistical software was used to analyze the data.Results:The positive expressions of PAH-DNA adducts in lung cancer tissues, adjacent cancer tissues and normal lung tissues of Xuanwei female lung cancer patients were 90%, 80% and 65%, respectively.They were higher than the positive expressions of PAH-DNA adducts in Xuanwei male lung cancer patients (35%, 30% and 30%) and non-Xuanwei female lung cancer patients (20%, 15% and 10%; P<0.01).The expression of PAH-DNA adducts in lung tissues of Xuanwei female benign lung lesion patients (70%) was higher than that in non-Xuanwei female benign lung lesion patients (10%).With the direction changing from cancer tissues, adjacent cancer tissues to normal lung tissues, the expression of PAH-DNA adducts was decreased but had no statistical difference (P>0.05).Conclusion:The expression of PAH-DNA adducts in lung tissues of Xuanwei females was higher than that in Xuanwei males and non-Xuanwei females.

Impact of Air Pollution on Skin

Recent articles reviewed the molecular damaging pathway of various pollutants especially PM2.5 particles and polycylic aromatic hydrocarbon. AhR （aryl hydrocarbon receptor） pathway, tobacco-similar pathway, oxidation, as well as epigenetics pathway were reviewed. Based on the working mechanism, the reported study method and methodology used in Ashland lab were reviewed, including cell level experiment, through reconstructed human skin to in vivo study.

Chiral Pool Guided Syntheses of Polycyclic Natural Products

Chiral pools have been commonly utilized as inexpensive and versatile building blocks in organic synthesis.In this account,we provide an overview of our recent synthetic efforts toward polycyclic natural products using chiral natural small molecules(chiral pool)as starting materials.Key achievements during our syntheses include intermolecular Diels-Alder/intramolecular hetero-Diels-Alder cascade to enable modification of previously proposed biosynthetic pathway of bolivianine,Diels-Alder-type dimerization through a conjugated furanyl diene intermediate to achieve three types of lindenane[4+2]dimers,Iron-catalyzed reductive aldol reaction to construct core of rumphellclovane E and[3,3]-rearrangement along with vinylo-gous Pummerer rearrangement to make epoxyquinol substructure of JBIR-23/-24.In the final,the outlook is also presented.