A new tdterpenoid glycoside, 3-β-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabipyranosyl（1 → 3）-6-β- xylopyranosyl（1 → 4）-[β-D-apiofuranosyl（1 → 3）]-Ct-L-rhamnopyranosyl（1 → 2）-[α-L-rhamnopyranosyl...A new tdterpenoid glycoside, 3-β-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabipyranosyl（1 → 3）-6-β- xylopyranosyl（1 → 4）-[β-D-apiofuranosyl（1 → 3）]-Ct-L-rhamnopyranosyl（1 → 2）-[α-L-rhamnopyranosyl（1 → 3）]-β-D-fucopyranosyl ester （1） was isolated from the Polygala tenuifolia Willd., together with two known saponins, including polygalasaponinXXIV （2） and polygalasaponinXXVIII （3）. The structure of new compound was elucidated by spectroscopic methods.展开更多
[Objectives] Taking Polygala tenuifolia Willd. seeds as experimental material, the changes of physiological and biochemical characteristics of seeds under different storage temperatures and seed moisture contents were...[Objectives] Taking Polygala tenuifolia Willd. seeds as experimental material, the changes of physiological and biochemical characteristics of seeds under different storage temperatures and seed moisture contents were studied. [Methods] The seeds of P. tenuifolia Willd. with moisture contents of 3%, 5% and 15% were stored at-10 ℃, 0 ℃, 20 ℃ and 36 ℃, respectively, and the storage time was one year. The physiological and biochemical characteristics of the seeds, such as conductivity, SOD activity, POD activity, and soluble sugar content were studied.[Results] After one-year storage, as the storage temperature and seed moisture content increased, the relative conductivity, MDA content and soluble sugar content of P. tenuifolia Willd. seeds increased, while the SOD activity, POD activity, and protein content declined. This phenomenon was most obvious in the P. tenuifolia Willd. seeds with moisture content of 15%. [Conclusions] This study will provide reference for seed storage of medicinal plants.展开更多
The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifo/ia Willd （PTW） in vitro. The root of PTW was extracted in distil...The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifo/ia Willd （PTW） in vitro. The root of PTW was extracted in distilled water. Normal human spermatozoa were used to assess the spermicidal activity （Sander-Cramer assay） of the extract from the PTW root. The hypo-osmotic swelling （HOS） test and the eosin Y （EY） staining were used to detect the integrity of sperm membrane and vitality. The sperm chromatin dispersion （SCD） test was performed to determine sperm DNA integrity. N-9 was used as a reference standard and semen added to physiological saline was used as the control. Semen samples were donated by 42 healthy fertile men. The crude extract from the root of PTW could immobilize and kill 100% spermatozoa within 20 s in vitro at the concentrations of 20.0 and 10.0 mg/ml; at the concentration of 5.0 mg/ml, spermatozoa were immobilized in （39.5±3.2） s. In the groups of the crude extract from the root of PTW and N-9 solution the rate of the normal HOS （tails swollen） and the white head （unstained） was 0%, and the rate of the abnormal HOS （tails unswollen） and red head （stained） was 100%. Sperm DNA fragmentation showed no change in exposure to the crude extract from the root of PTW and N-9 solution. The sperm revival test did not show any spermatozoa that recovered their motilities. The rapid spermicidal activity of the crude extract from the root of PTW in vitro may occur by the disruption of the sperm membrane integrity.展开更多
基金the National Nature Science Foundation of China (No.30772890)the Collaborative Education,Research and Development Project of Beijing Municipal Commission of Education and the Key Project of Chinese Ministry of Education (No.108132).
文摘A new tdterpenoid glycoside, 3-β-O-β-D-glucopyranosyl presenegenin 28-O-α-L-arabipyranosyl（1 → 3）-6-β- xylopyranosyl（1 → 4）-[β-D-apiofuranosyl（1 → 3）]-Ct-L-rhamnopyranosyl（1 → 2）-[α-L-rhamnopyranosyl（1 → 3）]-β-D-fucopyranosyl ester （1） was isolated from the Polygala tenuifolia Willd., together with two known saponins, including polygalasaponinXXIV （2） and polygalasaponinXXVIII （3）. The structure of new compound was elucidated by spectroscopic methods.
文摘[Objectives] Taking Polygala tenuifolia Willd. seeds as experimental material, the changes of physiological and biochemical characteristics of seeds under different storage temperatures and seed moisture contents were studied. [Methods] The seeds of P. tenuifolia Willd. with moisture contents of 3%, 5% and 15% were stored at-10 ℃, 0 ℃, 20 ℃ and 36 ℃, respectively, and the storage time was one year. The physiological and biochemical characteristics of the seeds, such as conductivity, SOD activity, POD activity, and soluble sugar content were studied.[Results] After one-year storage, as the storage temperature and seed moisture content increased, the relative conductivity, MDA content and soluble sugar content of P. tenuifolia Willd. seeds increased, while the SOD activity, POD activity, and protein content declined. This phenomenon was most obvious in the P. tenuifolia Willd. seeds with moisture content of 15%. [Conclusions] This study will provide reference for seed storage of medicinal plants.
基金Project (No.2008-01) supported by the Foundation of Shandong Provincial Committee Family Planning Development,China
文摘The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifo/ia Willd （PTW） in vitro. The root of PTW was extracted in distilled water. Normal human spermatozoa were used to assess the spermicidal activity （Sander-Cramer assay） of the extract from the PTW root. The hypo-osmotic swelling （HOS） test and the eosin Y （EY） staining were used to detect the integrity of sperm membrane and vitality. The sperm chromatin dispersion （SCD） test was performed to determine sperm DNA integrity. N-9 was used as a reference standard and semen added to physiological saline was used as the control. Semen samples were donated by 42 healthy fertile men. The crude extract from the root of PTW could immobilize and kill 100% spermatozoa within 20 s in vitro at the concentrations of 20.0 and 10.0 mg/ml; at the concentration of 5.0 mg/ml, spermatozoa were immobilized in （39.5±3.2） s. In the groups of the crude extract from the root of PTW and N-9 solution the rate of the normal HOS （tails swollen） and the white head （unstained） was 0%, and the rate of the abnormal HOS （tails unswollen） and red head （stained） was 100%. Sperm DNA fragmentation showed no change in exposure to the crude extract from the root of PTW and N-9 solution. The sperm revival test did not show any spermatozoa that recovered their motilities. The rapid spermicidal activity of the crude extract from the root of PTW in vitro may occur by the disruption of the sperm membrane integrity.
