A sensitive and specific reversed-phase high performance liquid chromatography （RP-HPLC） method was established for the simultaneous quantitative determination of eight active components, two stilbenes （resveratrol...A sensitive and specific reversed-phase high performance liquid chromatography （RP-HPLC） method was established for the simultaneous quantitative determination of eight active components, two stilbenes （resveratrol, polydatin） and six flavonoids （rutin, quercilrin, quercetin, luteotin, isoorientin, kaempferol）, in Polygonum multiflorum Thunb. The chromatographic separation was performed on a Kromasil C18 column （250 mm^4.6 mm, 5 ~tm）, and gradient elution was carried out with water-methanol as the mobile phase at a flow rate of 1 mL/min. Stilbenes and flavonoids were respectively detected at 320 nm and 350 nm with DAD. The correlation coefficients of all the calibration curves were found to be higher than 0.9995. The average recoveries were ranged from 96.8% to 102.5% with RSD less than 4.8% for these components. RP-HPLC was validated to be a robust method for the quantitative determination of active components in Polygonum multiflorum Thunb, and it could be used in the quality control of this traditional medicine.展开更多
目的：建立以高效液相法测定何首乌水提液中二苯乙烯苷含量的方法。方法：色谱柱为Agilent Eclipse Plus C18（100 mm ×4.6 mm,3.5μm）,柱温为25℃,流动相为乙腈∶水=18∶82,流速为1.0 ml·min^-1,检测波长为320 nm,进样量为8...目的：建立以高效液相法测定何首乌水提液中二苯乙烯苷含量的方法。方法：色谱柱为Agilent Eclipse Plus C18（100 mm ×4.6 mm,3.5μm）,柱温为25℃,流动相为乙腈∶水=18∶82,流速为1.0 ml·min^-1,检测波长为320 nm,进样量为8μl。结果：二苯乙烯苷在38~237.5μg·ml^-1（r=0.9994）范围内线性关系良好,回收率为98.32%,RSD=1.59%。结论：本法简单、灵敏、专属性强,可用于何首乌中二苯乙烯苷的含量测定。展开更多
文摘A sensitive and specific reversed-phase high performance liquid chromatography （RP-HPLC） method was established for the simultaneous quantitative determination of eight active components, two stilbenes （resveratrol, polydatin） and six flavonoids （rutin, quercilrin, quercetin, luteotin, isoorientin, kaempferol）, in Polygonum multiflorum Thunb. The chromatographic separation was performed on a Kromasil C18 column （250 mm^4.6 mm, 5 ~tm）, and gradient elution was carried out with water-methanol as the mobile phase at a flow rate of 1 mL/min. Stilbenes and flavonoids were respectively detected at 320 nm and 350 nm with DAD. The correlation coefficients of all the calibration curves were found to be higher than 0.9995. The average recoveries were ranged from 96.8% to 102.5% with RSD less than 4.8% for these components. RP-HPLC was validated to be a robust method for the quantitative determination of active components in Polygonum multiflorum Thunb, and it could be used in the quality control of this traditional medicine.
文摘目的：建立以高效液相法测定何首乌水提液中二苯乙烯苷含量的方法。方法：色谱柱为Agilent Eclipse Plus C18（100 mm ×4.6 mm,3.5μm）,柱温为25℃,流动相为乙腈∶水=18∶82,流速为1.0 ml·min^-1,检测波长为320 nm,进样量为8μl。结果：二苯乙烯苷在38~237.5μg·ml^-1（r=0.9994）范围内线性关系良好,回收率为98.32%,RSD=1.59%。结论：本法简单、灵敏、专属性强,可用于何首乌中二苯乙烯苷的含量测定。
