Inactivated cell catalysis is one of several central techniques in green biomanufacturing realm.However,the instability and leakage of enzymes in inactivated cell severely restrict the practical applications of inacti...Inactivated cell catalysis is one of several central techniques in green biomanufacturing realm.However,the instability and leakage of enzymes in inactivated cell severely restrict the practical applications of inactivated cell catalysis.Constructing armor on the surface of inactivated cells affords a feasible and effective strategy to enhance the stability of cells while commonly lowering the permeability.Herein,polymer-silica hybrid armor(PSHA)is directly generated on the surface of enzyme-containing cells.The branched structure of PEI enables higher porosity of cell@PSHA,exhibiting 1.52-fold enhancement in substrate permeability by contrast with cell@silica armor(SA).The electrostatic interactions(NH_(3)^(+)with O^(−))and hydrogen bonding(N⋯H or O⋯H)interactions between structural units enables higher stability of cell@PSHA,showing 3.13-fold elevation in Young's modulus compared with cell@SA.As a result,the cell@PSHA can catalyze continuous conversion of starch to tagatose for 15 batches over 969 h,with an average yield of 77.76 g L^(−1).展开更多
Hollow silica nanospheres were synthesized by silyl functionalized poly(vinyl benzyl chloride)(PVBC) latex nanoparticles via surface-initiated atom transfer radical polymerization(ATRP) of 3-(trimethoxysilyl)propyl me...Hollow silica nanospheres were synthesized by silyl functionalized poly(vinyl benzyl chloride)(PVBC) latex nanoparticles via surface-initiated atom transfer radical polymerization(ATRP) of 3-(trimethoxysilyl)propyl methacrylate(TMSPM),followed by polycondensation with tetraethoxysi-lane(TEOS) in the ethanol-ammonia and removal of the PVBC cores by thermal decomposition.Transmission electron microscopy(TEM) were used to characterize the intermediate products and the hollow nanospheres.展开更多
基金the National Key Research and Development Program of China(grant No.2021YFC2102300)National Natural Science Foundation of China(grant No.22122809)+2 种基金Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(grant No.TSBICIP-KJGG-003)Open Funding Project of the State Key Laboratory of Biochemical Engineering,China(grant No.2020 KF-06)Haihe Laboratory of Sustainable Chemical Transformations,and Program of Introducing Talents of Discipline to。
文摘Inactivated cell catalysis is one of several central techniques in green biomanufacturing realm.However,the instability and leakage of enzymes in inactivated cell severely restrict the practical applications of inactivated cell catalysis.Constructing armor on the surface of inactivated cells affords a feasible and effective strategy to enhance the stability of cells while commonly lowering the permeability.Herein,polymer-silica hybrid armor(PSHA)is directly generated on the surface of enzyme-containing cells.The branched structure of PEI enables higher porosity of cell@PSHA,exhibiting 1.52-fold enhancement in substrate permeability by contrast with cell@silica armor(SA).The electrostatic interactions(NH_(3)^(+)with O^(−))and hydrogen bonding(N⋯H or O⋯H)interactions between structural units enables higher stability of cell@PSHA,showing 3.13-fold elevation in Young's modulus compared with cell@SA.As a result,the cell@PSHA can catalyze continuous conversion of starch to tagatose for 15 batches over 969 h,with an average yield of 77.76 g L^(−1).
文摘Hollow silica nanospheres were synthesized by silyl functionalized poly(vinyl benzyl chloride)(PVBC) latex nanoparticles via surface-initiated atom transfer radical polymerization(ATRP) of 3-(trimethoxysilyl)propyl methacrylate(TMSPM),followed by polycondensation with tetraethoxysi-lane(TEOS) in the ethanol-ammonia and removal of the PVBC cores by thermal decomposition.Transmission electron microscopy(TEM) were used to characterize the intermediate products and the hollow nanospheres.
