Objective: "Same treatment for different diseases" is a unique treatment strategy under the guidance of traditional Chinese medicine(TCM) theory.Codonopsis Radix(Codonopsis pilosula,Dangshen in Chinese)with ...Objective: "Same treatment for different diseases" is a unique treatment strategy under the guidance of traditional Chinese medicine(TCM) theory.Codonopsis Radix(Codonopsis pilosula,Dangshen in Chinese)with spleen-fortifying effect was employed to understand the strategy of "Same treatment for different diseases",based on its common mechanism in the treatment of gastric diseases including gastric ulcer,gastritis and gastric cancer via network pharmacology research.Methods:Network pharmacology research methods were used to analyze the interaction network and potential mechanisms of Dangshen in treating gastric ulcer,gastritis and gastric cancer.The active components and their target proteins of Dangshen were integrated from TCMSP,BATMAN-TCM databases.The targets of gastric ulcer,gastritis and gastric cancer were collected through GeneCards,PubMed,TDD and DisGeNET Database.Through screening,the key components and the key targets of Dangshen in treating gastric ulcer,gastritis and gastric cancer were obtained.After KEGG pathway analysis and GO analysis,the important pathways and biological processes were analyzed.Results:Through data and literature mining,the common and specific pharmaceutical effects and mechanism of Dangshen were summarized in these three gastric lesions.It was shown that Dangshen mainly acted on gastric ulcer,gastritis and gastric cancer through the overall regulation of the PI3K-AkT signaling pathway.With the development of the disease,it will gradually increase the control of inflammation through TNF,NF-κB and other inflammation-related signaling pathways to reduce inflammatory damage.For tumorigenesis,it pays more attention to inhibiting the ErbB signaling pathways to reduce the proliferation and migration of tumor cells.In addition,Dangshen's regulation of HIF-1 signaling pathway may also be beneficial for the treatment of gastric ulcer,gastritis and gastric cancer.Conclusion:Dangshen achieves spleen-fortifying effect on gastric diseases including gastric ulcer,gastritis and gastric cancer through multiple targets in multiple pathways,especially PI3K-AKT pathway and HIF-1 pathway.It could provide a scientific basis for understanding the strategy of "Same treatment for different diseases" in traditional Chinese medicine.展开更多
目的构建有效的化合物-靶标网络以科学解释党参治疗胃溃疡的分子机制。方法通过中药系统药理学数据库和分析平台(TCMSP)收集党参的活性成分;通过文献挖掘和多数据库搜索收集了党参的潜在靶标;通过Gencard数据库筛选与胃溃疡、胃炎、胃...目的构建有效的化合物-靶标网络以科学解释党参治疗胃溃疡的分子机制。方法通过中药系统药理学数据库和分析平台(TCMSP)收集党参的活性成分;通过文献挖掘和多数据库搜索收集了党参的潜在靶标;通过Gencard数据库筛选与胃溃疡、胃炎、胃黏膜修复的相关基因,使用Cytoscape软件构建药物靶标-疾病靶标复合网络,通过String数据库构建药物成分潜在靶标和疾病靶标的PPI网络;并通过生物信息注释数据库(DAVID)信号路径分析目标基因的功能。结果从党参中134个化合物中筛选出具有良好ADME的21种化合物,其涉及的靶点有118个可能与胃溃疡、胃炎、胃黏膜修复相关;通过调节Fluid shear stress and atherosclerosis、AGE-RAGE、IL-17等信号通路来发挥治疗胃溃疡的作用。结论党参主要是通过多靶点多通路多途径来发挥治疗胃溃疡的作用,该研究为开展党参治疗胃溃疡的作用机制研究提供了新思路和新方法。展开更多
基金financially supported by the National Key Research and Development Program of China (2018YFC1706300 and 2018YFC17063005)the Fundamental Research Funds for the Central Universities (lzujbky-2019-92, lzujbky-2020-sp13)+2 种基金the Project for Longyuan Youth Innovation and Entrepreneurship TalentScience the Special funds for Innovation and Guidance of Gansu (2017ZX-02)the Science-Technology Plan Project from Lanzhou Science and Technology Bureau (2017-RC115)。
文摘Objective: "Same treatment for different diseases" is a unique treatment strategy under the guidance of traditional Chinese medicine(TCM) theory.Codonopsis Radix(Codonopsis pilosula,Dangshen in Chinese)with spleen-fortifying effect was employed to understand the strategy of "Same treatment for different diseases",based on its common mechanism in the treatment of gastric diseases including gastric ulcer,gastritis and gastric cancer via network pharmacology research.Methods:Network pharmacology research methods were used to analyze the interaction network and potential mechanisms of Dangshen in treating gastric ulcer,gastritis and gastric cancer.The active components and their target proteins of Dangshen were integrated from TCMSP,BATMAN-TCM databases.The targets of gastric ulcer,gastritis and gastric cancer were collected through GeneCards,PubMed,TDD and DisGeNET Database.Through screening,the key components and the key targets of Dangshen in treating gastric ulcer,gastritis and gastric cancer were obtained.After KEGG pathway analysis and GO analysis,the important pathways and biological processes were analyzed.Results:Through data and literature mining,the common and specific pharmaceutical effects and mechanism of Dangshen were summarized in these three gastric lesions.It was shown that Dangshen mainly acted on gastric ulcer,gastritis and gastric cancer through the overall regulation of the PI3K-AkT signaling pathway.With the development of the disease,it will gradually increase the control of inflammation through TNF,NF-κB and other inflammation-related signaling pathways to reduce inflammatory damage.For tumorigenesis,it pays more attention to inhibiting the ErbB signaling pathways to reduce the proliferation and migration of tumor cells.In addition,Dangshen's regulation of HIF-1 signaling pathway may also be beneficial for the treatment of gastric ulcer,gastritis and gastric cancer.Conclusion:Dangshen achieves spleen-fortifying effect on gastric diseases including gastric ulcer,gastritis and gastric cancer through multiple targets in multiple pathways,especially PI3K-AKT pathway and HIF-1 pathway.It could provide a scientific basis for understanding the strategy of "Same treatment for different diseases" in traditional Chinese medicine.
文摘目的构建有效的化合物-靶标网络以科学解释党参治疗胃溃疡的分子机制。方法通过中药系统药理学数据库和分析平台(TCMSP)收集党参的活性成分;通过文献挖掘和多数据库搜索收集了党参的潜在靶标;通过Gencard数据库筛选与胃溃疡、胃炎、胃黏膜修复的相关基因,使用Cytoscape软件构建药物靶标-疾病靶标复合网络,通过String数据库构建药物成分潜在靶标和疾病靶标的PPI网络;并通过生物信息注释数据库(DAVID)信号路径分析目标基因的功能。结果从党参中134个化合物中筛选出具有良好ADME的21种化合物,其涉及的靶点有118个可能与胃溃疡、胃炎、胃黏膜修复相关;通过调节Fluid shear stress and atherosclerosis、AGE-RAGE、IL-17等信号通路来发挥治疗胃溃疡的作用。结论党参主要是通过多靶点多通路多途径来发挥治疗胃溃疡的作用,该研究为开展党参治疗胃溃疡的作用机制研究提供了新思路和新方法。
