BACKGROUND Colon cancer remains a leading cause of death globally.Pomolic acid(PA)can be separated from the ethyl acetate fraction of achyrocline satureioides.AIM To determine the effects of PA and its glucopyranose e...BACKGROUND Colon cancer remains a leading cause of death globally.Pomolic acid(PA)can be separated from the ethyl acetate fraction of achyrocline satureioides.AIM To determine the effects of PA and its glucopyranose ester,pomolic acid-28-O-β-D-glucopyranosyl ester(PAO),on colon cancer HT-29 cells.METHODS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was used to measure cell viability.Apoptosis was detected via hoechst 33342 staining.PI single staining was identified by flow cytometry to determine the cycle and scratch assay was used to observe the migration of HT-29 cells.The levels of mRNA and proteins were evaluated by q polymerase chain reaction and western blotting,respectively.RESULTS PA and PAO considerably inhibited the growth of the HT-29 cell line in a time and dose-dependent manner.After the administration of PA and PAO for 24 and 48 h,cell apoptosis was significantly promoted and HT-29 cells were arrested in the G0/G1 stage.The Bax/Bcl2 ratio was also increased,which activated cysteinyl aspartate specific proteinase 3,leading to apoptosis;it also increased the expression of light chain 3 II/I and Beclin1,which activated autophagy and caused cell death.This in turn increased the expression of p62 to promote cell apoptosis,inhibiting the levels of signal transducer and activator of transcription 3(STAT3)and p-STAT3,suppressing the level of Bcl2,and promoting cell.CONCLUSION Both PA and PAO provide novel therapeutic strategies for treating colorectal cancer.展开更多
Herbal medicinal products have a long-standing therapeutic record. To ensure the quality of herbal products specific identification tests which allow experts to discriminate related species and/or potential adulterant...Herbal medicinal products have a long-standing therapeutic record. To ensure the quality of herbal products specific identification tests which allow experts to discriminate related species and/or potential adulterants/substitutes are required. The purpose of the research was to recommend an original and simple method for the separation of closely related five triterpenic acids (ursolic, oleanolic, tormentic, euscaphic, pomolic acid) and its application to chemotaxonomy studies. 17 standard samples of Potentilla species and 3 test samples were chromatographed (with or without prechromatographic derivatization) on silica gel plates using the mobile phase: chloroform-diethyl ether-methanol-formic acid (30:10:1:0.2 v/v/v/v);they were subsequently derivatized, and visualized in UV 366 nm light. After images received pre-treatment (montaging stack, removal of noise, background subtract, horizontal equalization, two types of warping) exploratory analysis of the investigated Potentilla species fingerprints was processed. The method without prechromatographic derivatization was used to create differential fingerprints. Good results were obtained by the application of r-project iterative algorithm for subtract background and our simple algorithm for warping to image processing of TLC chromatograms for the first time.展开更多
Objective To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods The compounds were isolated and purified by column chromatography and their structures were identified on ...Objective To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. Results Five compounds were isolated and identified as 3β-hydroxy-28-norurs-17,19,21-trien (1), 3β-hydroxy-28-norurs-12,17-dien (2), 3β,19α-dihydroxyurs-13(18)- en-28-oic acid (3), 3β-[(α-L-arabin-opyranosyl) oxy]-28-norurs-12,17-dien (4), and pomolic acid (5). Conclusion Compounds 1, 3, and 4 are novel compounds belong to triterpenoids and triterpenoid saponins, named as sanguisorbigenins Z, Y1 , and Y2 , respectively.展开更多
文摘BACKGROUND Colon cancer remains a leading cause of death globally.Pomolic acid(PA)can be separated from the ethyl acetate fraction of achyrocline satureioides.AIM To determine the effects of PA and its glucopyranose ester,pomolic acid-28-O-β-D-glucopyranosyl ester(PAO),on colon cancer HT-29 cells.METHODS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was used to measure cell viability.Apoptosis was detected via hoechst 33342 staining.PI single staining was identified by flow cytometry to determine the cycle and scratch assay was used to observe the migration of HT-29 cells.The levels of mRNA and proteins were evaluated by q polymerase chain reaction and western blotting,respectively.RESULTS PA and PAO considerably inhibited the growth of the HT-29 cell line in a time and dose-dependent manner.After the administration of PA and PAO for 24 and 48 h,cell apoptosis was significantly promoted and HT-29 cells were arrested in the G0/G1 stage.The Bax/Bcl2 ratio was also increased,which activated cysteinyl aspartate specific proteinase 3,leading to apoptosis;it also increased the expression of light chain 3 II/I and Beclin1,which activated autophagy and caused cell death.This in turn increased the expression of p62 to promote cell apoptosis,inhibiting the levels of signal transducer and activator of transcription 3(STAT3)and p-STAT3,suppressing the level of Bcl2,and promoting cell.CONCLUSION Both PA and PAO provide novel therapeutic strategies for treating colorectal cancer.
文摘Herbal medicinal products have a long-standing therapeutic record. To ensure the quality of herbal products specific identification tests which allow experts to discriminate related species and/or potential adulterants/substitutes are required. The purpose of the research was to recommend an original and simple method for the separation of closely related five triterpenic acids (ursolic, oleanolic, tormentic, euscaphic, pomolic acid) and its application to chemotaxonomy studies. 17 standard samples of Potentilla species and 3 test samples were chromatographed (with or without prechromatographic derivatization) on silica gel plates using the mobile phase: chloroform-diethyl ether-methanol-formic acid (30:10:1:0.2 v/v/v/v);they were subsequently derivatized, and visualized in UV 366 nm light. After images received pre-treatment (montaging stack, removal of noise, background subtract, horizontal equalization, two types of warping) exploratory analysis of the investigated Potentilla species fingerprints was processed. The method without prechromatographic derivatization was used to create differential fingerprints. Good results were obtained by the application of r-project iterative algorithm for subtract background and our simple algorithm for warping to image processing of TLC chromatograms for the first time.
基金National Natural Science Foundation of China-Study on the Mechanism of Diyutan Processing (30772786)
文摘Objective To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. Results Five compounds were isolated and identified as 3β-hydroxy-28-norurs-17,19,21-trien (1), 3β-hydroxy-28-norurs-12,17-dien (2), 3β,19α-dihydroxyurs-13(18)- en-28-oic acid (3), 3β-[(α-L-arabin-opyranosyl) oxy]-28-norurs-12,17-dien (4), and pomolic acid (5). Conclusion Compounds 1, 3, and 4 are novel compounds belong to triterpenoids and triterpenoid saponins, named as sanguisorbigenins Z, Y1 , and Y2 , respectively.