This standard operating procedure stipulated the natural condition of pro- ducing area, cultivation technique, disease and pest control, harvest, quality stan- dard, packaging, transportation and storage of H. cordata...This standard operating procedure stipulated the natural condition of pro- ducing area, cultivation technique, disease and pest control, harvest, quality stan- dard, packaging, transportation and storage of H. cordata, in order to provide a ba- sis for the standard cultivation of H. cordata.展开更多
[Objective] The study was to screen the active part from roots of Macleaya cordata. [Method] Dipping method was used to study the contact activity of ethanol extract and 4 kinds of extracts by petroleum, ethyl acetate...[Objective] The study was to screen the active part from roots of Macleaya cordata. [Method] Dipping method was used to study the contact activity of ethanol extract and 4 kinds of extracts by petroleum, ethyl acetate, n-butanol and water from the root of M. cordata on 2nd and 6th instars larvae of T. motlitorlin（L.）. [Result] The corrected mortality rate of T. motlitorlin increased with the increase of extract concentration. When the concentration was 100 mg/ml, the contact activity of ethyl acetate extract against 2nd instar larvae of T. motlitorlin was the highest, the corrected mortality rate at 48 h reached 80.95%, and LC50 was 65.532 9 mg/ml. The overall performance of insecticidal activity was as follows： ethyl acetate layer petroleum layer = n-butanol layer total extracts （ethanol） water layer. The contact toxicity of ethanol extract and petroleum extract against 6th instar larvae of T. motlitorlin was the highest, and the corrected mortality rate at 48 h reached 71.42% and 66.67%, LC50 were 83.899 8 and 86.687 7 mg/ml, respectively. The overall performance of insecticidal activity was as follows： total extracts （ethanol） petroleum layer ethyl acetate layer n-butanol layer water layer. [Conclusion] The insecticidal active components of M. cordata root were distributed within the polarity range of petroleum and ethyl acetate, and the part could be further separated and identified.展开更多
Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB ir...Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB irradiation. The results showed that the extracts had strong UVA and UVB absorption ability and hydroxyl free radical scavenging ability. The S. japonica L. polysaccharide showed strong anti-UVB irradiation ability according to the cell viability experiment. It suggests that the H. cordata flavonoid and S. japonica L. polysaccharide could be combined as a candidate of anti-UV sun-screening supplement and compensate for the shortcomings of chemical sunscreens against UVA ray.展开更多
Background Eutrophication and acid rain are two threats that many water bodies must contend with.Eutrophication and climate change have accelerated widespread outbreaks of cyanobacterial blooms as both have become mor...Background Eutrophication and acid rain are two threats that many water bodies must contend with.Eutrophication and climate change have accelerated widespread outbreaks of cyanobacterial blooms as both have become more severe.Pontederia cordata,a garden ornamental plant,can inhibit some algae growth and remove total nitrogen(TN)and total phosphorus(TP)from the water.In this study,we investigated how simulated acid rain and eutrophication co-pollution affected P.cordata’s growth physiology and ability to inhibit algae growth.Results Under mild eutrophication(2 mg·L^(−1)TN,0.4 mg·L^(−1)TP,and 15 mg·L^(−1)CODMn)or weak acid rain(pH=5.0),P.cordata alleviated the degree of cell membrane lipid peroxidation by stabilizing superoxide dismutase(SOD)and catalase(CAT)activities in the leaves,allowing for normal plant growth.Under mild eutrophication and acid rain conditions,cultured P.cordata water samples maintained strong algae inhibition by reducing the Chl a content and SOD activity of Microcystis aeruginosa cells.Compound stress where acid rain was the primary inhibitory factor along with moderate or severe eutrophication inhibited P.cordata growth,which probably reduced the input of algae-inhibiting allelochemicals,thus reducing its ability to inhibit algae.Conclusions In summary,P.cordata has application potential in mild eutrophic water and acid rain(pH≥4).These findings provide guidance for further research on phytoremediation and algae control in scenarios of compound pollution.展开更多
基金Supported by Key Research and Development Plan(Agricultural Support Field)of Hunan Province in 2015(2015NK3016)
文摘This standard operating procedure stipulated the natural condition of pro- ducing area, cultivation technique, disease and pest control, harvest, quality stan- dard, packaging, transportation and storage of H. cordata, in order to provide a ba- sis for the standard cultivation of H. cordata.
基金Supported by Hebei Science and Technology Research and Development Project(10220302D)
文摘[Objective] The study was to screen the active part from roots of Macleaya cordata. [Method] Dipping method was used to study the contact activity of ethanol extract and 4 kinds of extracts by petroleum, ethyl acetate, n-butanol and water from the root of M. cordata on 2nd and 6th instars larvae of T. motlitorlin（L.）. [Result] The corrected mortality rate of T. motlitorlin increased with the increase of extract concentration. When the concentration was 100 mg/ml, the contact activity of ethyl acetate extract against 2nd instar larvae of T. motlitorlin was the highest, the corrected mortality rate at 48 h reached 80.95%, and LC50 was 65.532 9 mg/ml. The overall performance of insecticidal activity was as follows： ethyl acetate layer petroleum layer = n-butanol layer total extracts （ethanol） water layer. The contact toxicity of ethanol extract and petroleum extract against 6th instar larvae of T. motlitorlin was the highest, and the corrected mortality rate at 48 h reached 71.42% and 66.67%, LC50 were 83.899 8 and 86.687 7 mg/ml, respectively. The overall performance of insecticidal activity was as follows： total extracts （ethanol） petroleum layer ethyl acetate layer n-butanol layer water layer. [Conclusion] The insecticidal active components of M. cordata root were distributed within the polarity range of petroleum and ethyl acetate, and the part could be further separated and identified.
基金Supported by College Students'Practice Innovation Training Project in Henan Province(No.201711834006)Henan Provincial Department of Education(No.15A350008)
文摘Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB irradiation. The results showed that the extracts had strong UVA and UVB absorption ability and hydroxyl free radical scavenging ability. The S. japonica L. polysaccharide showed strong anti-UVB irradiation ability according to the cell viability experiment. It suggests that the H. cordata flavonoid and S. japonica L. polysaccharide could be combined as a candidate of anti-UV sun-screening supplement and compensate for the shortcomings of chemical sunscreens against UVA ray.
基金supported by the National Natural Science Foundation of China(No.31670698)the Project Funded by China Postdoctoral Science Foundation(No.2020M671509)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX23_1255).
文摘Background Eutrophication and acid rain are two threats that many water bodies must contend with.Eutrophication and climate change have accelerated widespread outbreaks of cyanobacterial blooms as both have become more severe.Pontederia cordata,a garden ornamental plant,can inhibit some algae growth and remove total nitrogen(TN)and total phosphorus(TP)from the water.In this study,we investigated how simulated acid rain and eutrophication co-pollution affected P.cordata’s growth physiology and ability to inhibit algae growth.Results Under mild eutrophication(2 mg·L^(−1)TN,0.4 mg·L^(−1)TP,and 15 mg·L^(−1)CODMn)or weak acid rain(pH=5.0),P.cordata alleviated the degree of cell membrane lipid peroxidation by stabilizing superoxide dismutase(SOD)and catalase(CAT)activities in the leaves,allowing for normal plant growth.Under mild eutrophication and acid rain conditions,cultured P.cordata water samples maintained strong algae inhibition by reducing the Chl a content and SOD activity of Microcystis aeruginosa cells.Compound stress where acid rain was the primary inhibitory factor along with moderate or severe eutrophication inhibited P.cordata growth,which probably reduced the input of algae-inhibiting allelochemicals,thus reducing its ability to inhibit algae.Conclusions In summary,P.cordata has application potential in mild eutrophic water and acid rain(pH≥4).These findings provide guidance for further research on phytoremediation and algae control in scenarios of compound pollution.
