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Radial profile of sap flow velocity in mature Xinjiang poplar(Populus alba L. var. pyramidalis) in Northwest China 被引量:10
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作者 HongZhong DANG TianShan ZHA +2 位作者 JinSong ZHANG Wei LI ShiZeng LIU 《Journal of Arid Land》 SCIE CSCD 2014年第5期612-627,共16页
Estimation of the transpiration rate for a tree is generally based on sap flow measurements within the hydro-active stem xylem. In this study, radial variation of sap flow velocity(Js) was investigated at five depth... Estimation of the transpiration rate for a tree is generally based on sap flow measurements within the hydro-active stem xylem. In this study, radial variation of sap flow velocity(Js) was investigated at five depths of the xylem(1, 2, 3, 5 and 8 cm under the cambium) in three mature Xinjiang poplar(Populus alba L. var. pyramidalis) trees grown at the Gansu Minqin National Studies Station for Desert Steppe Ecosystem from May to October 2011. Thermal dissipation probes of various lengths manufactured according to the Granier's design were installed into each tree for simultaneous observation of the radial patterns of Js through the xylem. The radial patterns were found to fit the four-parameter GaussAmp equation. The peak Js was about 27.02±0.95 kg/(dm2?d) at approximately 3 to 5 cm deep from the cambium of the three trees,and the lowest Js appeared at 1 cm deep in most of the time. Approximately 50% of the total sap flow in Xinjiang poplar occurred within one-third of the xylem from its outer radius, whereas 90% of the total sap flow occurred within two-fifth of the xylem. In addition, the innermost point of the xylem(at 8-cm depth), which appeared as the penultimate sap flow in most cases during the study period, was hydro-active with Js,8 of 7.55±3.83 kg/(dm2?d). The radial pattern of Js was found to be steeper in midday than in other time of the day, and steeper diurnal fluctuations were recorded in June, July and August(the mid-growing season). Maximum differences between the lowest Js(Js,1 or Js,8) and the highest Js(Js,3 or Js,5) from May through October were 12.41, 17.35, 16.30, 18.52, 12.60 and 16.04 g/(cm2?h), respectively. The time-dependent changes of Js along the radial profile(except at 1-cm depth) were strongly related to the reference evapotranspiration(ET0). Due to significant radial variability of Js, the mean daily sap flow at the whole-tree level could be over-estimated by up to 29.69% when only a single probe at depth of 2 cm was used. However, the accuracy of the estimation of sap flow in Xinjiang poplar could be significantly improved using a correction coefficient of 0.885. 展开更多
关键词 sap flow radial pattern populus alba L.var.pyramidalis reference evapotranspiration(ET0) Granier method
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An improved draft genome sequence of hybrid Populus alba×Populus glandulosa 被引量:2
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作者 Xiong Huang Song Chen +9 位作者 Xiaopeng Peng Eun-Kyung Bae Xinren Dai Guiming Liu Guanzheng Qu Jae-Heung Ko Hyoshin Lee Su Chen Quanzi Li Mengzhu Lu 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第4期1663-1672,共10页
Populus alba × P.glandulosa clone 84 K,derived from South Korea,is widely cultivated in China and used as a model in the molecular research of woody plants because of hi gh gene transformation efficiency.Here,we ... Populus alba × P.glandulosa clone 84 K,derived from South Korea,is widely cultivated in China and used as a model in the molecular research of woody plants because of hi gh gene transformation efficiency.Here,we combined63-fold coverage Illumina short reads and 126-fold coverage PacBio long reads to assemble the genome.Due to the hi gh heterozygosity level at 2.1% estimated by k-mer analysis,we exploited TrioCanu for genome assembly.The PacBio clean subreads of P.alba × P.glandulosa were separated into two parts according to the similarities,compared with the parental genomes of P.alba and P.glandulosa.The two parts of the subreads were assembled to two sets of subgenomes comprising subgenome A(405.31 Mb,from P.alba)and subgenome G(376.05 Mb,from P.glandulosa) with the contig N50 size of 5.43 Mb and 2.15 Mb,respectively.A high-quality P.alba × P.glandulosa genome assembly was obtained.The genome size was 781.36 Mb with the contig N50 size of 3.66 Mb and the longest contig was 19.47 Mb.In addition,a total of 176.95 Mb(43.7%),152.37 Mb(40.5%)of repetitive elements were identified and a total of 38,701 and 38,449 protein-coding genes were predicted in subgenomes A and G,respectively.For functional annotation,96.98% of subgenome A and 96.96% of subgenome G genes were annotated with public databases.This de novo assembled genome will facilitate systematic and comprehensive study,such as multi-omics analysis,in the model tree P.alba X P.glandulosa. 展开更多
关键词 Genome assembly Gene annotation Hybrid poplar populus alba×p.glandulosa cl.84 K
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Experimental study on characteristic curves of restoring forces of Populus alba var. pyramidalis 被引量:1
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作者 ZHOU Jian-zhong 《Forestry Studies in China》 CAS 2011年第4期315-320,共6页
Static tests under cyclic loading were carried out on Populus alba var. pyramidalis to determine its characteristic curves of restoring forces. The results show that P alba var. pyramidalis had good elastic performanc... Static tests under cyclic loading were carried out on Populus alba var. pyramidalis to determine its characteristic curves of restoring forces. The results show that P alba var. pyramidalis had good elastic performance. There was degradation of strength and stiffness under cyclic loading and unloading, where characteristic curves of restoring forces ofP. alba var. pyrarnidalis assumed a "flat and contrary S-shape" form. Simultaneously, P alba vat. pyramidalis showed typical characteristics of brittle destruction under large high peak loading and unloading. Furthermore, dynamic tests were carried out under wind loads to obtain dynamic displacement curves and dynamic strain curves. P alba var. pyramidalis reflected its stochastic dynamic performance in building up its resistance to the stochastic wind loads and its dynamically hysteretic properties. The dynamic response of this species was random and fuzzy under stochastic wind loads. This study has theoretical significance and reference value to research on the static characteristics and wind-induced dynamic performance of forests. 展开更多
关键词 populus alba var. pyramidalis characteristic curve of restoring forces degradation of strength and stiffness stochasticwind loads
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Waterlogging tolerance and wood properties of transgenic Populus alba×glandulosa expressing Vitreoscilla hemoglobin gene(Vgb)
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作者 Yiliang Li Weixi Zhang +3 位作者 Wenxu Zhu Bingyu Zhang Qinjun Huang Xiaohua Su 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第2期831-839,共9页
Because overexpression of Vitreoscilla hemoglobin gene(Vgb)gene in plants can enhance tolerance to waterlogging,here Vgb was inserted into Populus alba×glandulosa to investigate its expression and effects on grow... Because overexpression of Vitreoscilla hemoglobin gene(Vgb)gene in plants can enhance tolerance to waterlogging,here Vgb was inserted into Populus alba×glandulosa to investigate its expression and effects on growth and physiological responses to waterlogging stress in the transgenic poplars.Southern blotting and RT-PCR analysis of Vgb-transgenic P.alba×glandulosa showed that the Vgb gene was integrated into the genome of the V13-81 and V13-85 transgenic lines and expressed.In greenhouse waterlogging stress tests,mortality of the transgenic poplar was significant lower than that of nontransgenic plants with increasing treatment time from 2 to 22 days.The transgenic plants had higher chlorophyll content and less chloroplast damage than in the control plants.Additionally,starch accumulation increased,and growth was enhanced in the transgenic plants,suggesting that the Vgb-expressing lines had improved energy reserves.Field trials of the transgenic poplar suggested that Vgb expression promotes growth and influences wood quality.Taken together,our results suggest that the expression of Vgb can increase the accumulation of chlorophyll and starch in the transgenic poplar,improve its ability to endure flooding,and improve growth and wood quality of the transgenic plants. 展开更多
关键词 populus alba×glandulosa vgb gene Waterlogging stress GROWTH MICROSCOPY
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Survival and development of immature Harmonia axyridis (Pallas) feeding on Chaitophorus populeti (Panzer) propagated on transgenic Populus alba × P. glandulosa
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作者 ZHANG Xiao-fen CHEN Min +3 位作者 ZHANG Bin-yu LUO You-qing HOU Ying-jie SU Xiao-hua 《Forestry Studies in China》 CAS 2009年第3期164-167,共4页
Laboratory feeding experiments with the poplar aphid, Chaitophorus populeti (Panzer), feeding on transgenic poplar (P. alba × P. glandulosa) varieties C13-5 and C013-5, were carried out to study the effect of... Laboratory feeding experiments with the poplar aphid, Chaitophorus populeti (Panzer), feeding on transgenic poplar (P. alba × P. glandulosa) varieties C13-5 and C013-5, were carried out to study the effect of transgenic poplar on the ladybird Harmonia axyridis (Pallas). The mortality and development time of the immature stages, the eclosion rate and body mass of H. axyridis were measured. The results indicated that C. populeti feeding on different varieties of transgenic plants had no statistically significant ef- fect on the mortality ofH. axyridis larvae. The development time of larval and pupal stages were not significantly different between the two transgenic poplars and a non-transgenic poplar. Furthermore, the body mass and eclosion rate did not show any difference between the H. axyridis feeding on aphids reared on transgenic plants and those from non-transgenic plants. It is suggested that transgenic plants have no deleterious effect on the predatory ladybird. 展开更多
关键词 transgenic populus alba × P. glandulosa Harmonia axyridis (Pallas) Chaitophorus populeti (Panzer) ecological risk
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Single-cell RNA sequencing reveals a high-resolution cell atlas of xylem in Populus 被引量:7
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作者 Hui Li Xinren Dai +5 位作者 Xiong Huang Mengxuan Xu Qiao Wang Xiaojing Yan Ronald R.Sederoff Quanzi Li 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2021年第11期1906-1921,共16页
High-throughputsingle-cellRNAsequencing(sc RNA-seq) has advantages over traditional RNA-seq to explore spatiotemporal information on gene dynamic expressions in heterogenous tissues. We performed Drop-seq, a method fo... High-throughputsingle-cellRNAsequencing(sc RNA-seq) has advantages over traditional RNA-seq to explore spatiotemporal information on gene dynamic expressions in heterogenous tissues. We performed Drop-seq, a method for the dropwise sequestration of single cells for sequencing, on protoplasts from the differentiating xylem of Populus alba × Populus glandulosa. The sc RNA-seq profiled9,798 cells, which were grouped into 12 clusters.Through characterization of differentially expressed genes in each cluster and RNA in situ hybridizations,we identified vessel cells, fiber cells, ray parenchyma cells and xylem precursor cells. Diffusion pseudotime analyses revealed the differentiating trajectory of vessels, fiber cells and ray parenchyma cells and indicated a different differentiation process between vessels and fiber cells, and a similar differentiation process between fiber cells and ray parenchyma cells. We identified marker genes for each cell type(cluster) and key candidate regulators during developmental stages of xylem cell differentiation. Our study generates a high-resolution expression atlas of wood formation at the single cell level and provides valuable information on wood formation. 展开更多
关键词 differentiating trajectory differentiating xylem marker genes populus alba×populus glandulosa single-cell RNA-seq wood formation
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胡杨PeERF1基因提高转基因银腺杨84K耐旱性研究
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作者 葛晓兰 杜久军 +2 位作者 张磊 曲冠证 胡建军 《林业科学研究》 CSCD 北大核心 2023年第5期83-90,共8页
[目的]分析PeERF1基因在胡杨干旱胁迫下的作用和PeERF1转基因植株抗旱的生理适应机制,为进一步研究该基因在木本植物中的抗旱调控机制奠定基础。[方法]以胡杨为材料进行20%PEG6000模拟干旱(0、12和24 h)处理,对胡杨PeERF1基因进行时空... [目的]分析PeERF1基因在胡杨干旱胁迫下的作用和PeERF1转基因植株抗旱的生理适应机制,为进一步研究该基因在木本植物中的抗旱调控机制奠定基础。[方法]以胡杨为材料进行20%PEG6000模拟干旱(0、12和24 h)处理,对胡杨PeERF1基因进行时空表达模式分析。以非转基因(WT)、过表达35S::PeERF1转基因植株(PE)、显性抑制35S::PeERF1-SRDX转基因植株(SE)为试验材料,采用不同浓度PEG6000(对照组,20%)处理WT、PE和SE模拟干旱胁迫,并对其进行表达模式、生长性状和生理指标分析。[结果]研究结果表明PeERF1基因在胡杨叶中的表达水平最高,其次是茎和根。在正常状态下,转基因植株和WT生长性状、叶绿素含量、过氧化氢酶(CAT)、丙二醛(MDA)和过氧化物酶(POD)含量变化不大。在20%PEG6000处理后,PE转基因植株比WT表现出更好的生长状态,PE转基因植株的叶绿素含量、CAT和POD含量高于WT,PE转基因植株MDA含量低于WT。而SE转基因植株则表现出相反的性状。[结论]本研究结果初步显示干旱胁迫下,PeERF1基因转基因植株生长状态、叶绿素含量、过氧化氢酶、丙二醛和过氧化物酶等相关生理指标均发现显著变化。PeERF1对转基因杨树响应干旱起到了正向调控的作用。 展开更多
关键词 银腺杨 转基因 干旱胁迫 生长性状 生理指标
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银腺杨不同部位叶片形态、生理及转录差异分析 被引量:2
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作者 毕楷杰 孙耀国 +1 位作者 杨敏生 张军 《植物遗传资源学报》 CAS CSCD 北大核心 2023年第3期829-842,共14页
本研究以银腺杨成年树木为对象,测定了不同类型叶片形态、生理和基因表达差异,为揭示银腺杨的成熟效应提供参考。主要研究结果如下:(1)长枝叶和短枝叶的叶片形态差异显著,不同部位的叶片有很大程度的重叠。(2)不同部位叶片所含化合物差... 本研究以银腺杨成年树木为对象,测定了不同类型叶片形态、生理和基因表达差异,为揭示银腺杨的成熟效应提供参考。主要研究结果如下:(1)长枝叶和短枝叶的叶片形态差异显著,不同部位的叶片有很大程度的重叠。(2)不同部位叶片所含化合物差异显著,即:下部叶片的叶绿素a和叶绿素总含量显著大于上部和中部;下部叶片的SOD酶活性显著高于上部和中部,MDA含量表现为中部>上部>下部;下部叶片的淀粉含量显著低于上部和中部;不同部位叶片的IAA含量随着高度的降低而上升,ABA含量表现为中部>上部>下部;长枝叶的IAA和IAA/ABA显著大于短枝叶,ZR含量呈现相反的变化趋势。(3)基于转录组数据对差异显著基因进行筛选,不同部位叶片之间的差异显著基因数量少于不同类型叶片之间差异显著基因数量。(4)对各比较组的差异显著基因进行GO和KEGG富集分析,结果显示上部长枝叶相对于下部长枝叶(SC-vs-XC)、上部短枝叶相对于下部短枝叶(SD-vs-XD)差异显著基因显著富集到光合作用通路上,对其中的2个基因进行基因注释,发现这2个基因均编码PsbR蛋白,且下部叶片中基因表达量上调;对生长素转导过程进行分析,发现短枝叶中相关基因表达量均上调。 展开更多
关键词 银腺杨(populus alba×P.glandulosa) 叶片形态 生理特性 转录差异
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新疆杨树干液流的径向变化及时滞特征 被引量:17
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作者 党宏忠 杨文斌 +2 位作者 李卫 刘世增 张友焱 《生态学报》 CAS CSCD 北大核心 2015年第15期5110-5120,共11页
利用热扩散技术,对绿洲农田防护林新疆杨(Populus alba L.var.pyramidalis)大树边材5个深度处(1、2、3、5 cm和8 cm)的液流速率(Js)开展了连续两年的监测,结果表明:(1)以标准长度2 cm的探针测得的液流速率(Js-2)为参照,形成层下1、3、5... 利用热扩散技术,对绿洲农田防护林新疆杨(Populus alba L.var.pyramidalis)大树边材5个深度处(1、2、3、5 cm和8 cm)的液流速率(Js)开展了连续两年的监测,结果表明:(1)以标准长度2 cm的探针测得的液流速率(Js-2)为参照,形成层下1、3、5、8 cm处的液流速率(Js-1,Js-3,Js-5,Js-8)与Js-2间具有显著的相关性,回归系数分别为0.24—0.27、1.18—1.61、0.81—1.64和0.38—0.75,液流速率最大的位点在形成层下3—5 cm处,液流速率最小的位点在最外侧(1 cm)或最内侧(8 cm)处,径向差异明显。边材不同深处的液流传输具有较一致的日变化过程。(2)在同步观测的5项气象要素中,大气水汽压亏缺(VPD)和太阳辐射(Ra)与Js的回归系数均较大,是驱动液流进程的主导气象要素。大气蒸发潜力(ET0)集合了多种气象要素的信息,具有与Ra一致的日变化进程(启动、峰值时刻相同),可作为分析液流昼、夜过程的综合气象变量。(3)新疆杨边材中五个深度处Js的峰值时刻基本相同(Js-1的峰值较其它层次提前4—123 min),均明显滞后于Ra(时滞)并提前于VPD,在7月份的晴天,ET0、Js和VPD峰值出现的时刻分别大致在12:30、14:00和15:00。新疆杨时滞的大小存在有规律的季节变化,从6到10月份,Js与ET0峰值的时滞(ΔJ-E)逐渐增加,变化在70—110 min(2011)、70—128 min(2012)之间,但VPD与Js峰值的时滞(ΔJ-V)逐渐降低,变化在73—20 min(2011)、63—8 min(2012)之间,这表明在生长季的早期,热量因子(Ra)对新疆杨液流变化的驱动较强,而在生长季的末期,大气水汽因子VPD的驱动效应更突出。 展开更多
关键词 新疆杨(populus alba L.var.pyramidalis) 绿洲 树干液流 时滞
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液流—株间微型蒸渗仪法测定新疆杨蒸发蒸腾量适用性分析 被引量:11
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作者 屈艳萍 康绍忠 +1 位作者 王素芬 孙洪泉 《干旱地区农业研究》 CSCD 北大核心 2014年第3期88-94,共7页
采用液流—株间微型蒸渗仪法测定人工种植环境下新疆杨蒸发蒸腾量,并对此方法的适用性进行了分析。结果表明:在生长季5—10月,新疆杨蒸腾量具有明显的季节变化规律,总蒸发蒸腾量为508.26mm,蒸发量、蒸腾量所占比率分别为37.1%和62.9%;... 采用液流—株间微型蒸渗仪法测定人工种植环境下新疆杨蒸发蒸腾量,并对此方法的适用性进行了分析。结果表明:在生长季5—10月,新疆杨蒸腾量具有明显的季节变化规律,总蒸发蒸腾量为508.26mm,蒸发量、蒸腾量所占比率分别为37.1%和62.9%;树干液流速率与叶片蒸腾速率变化表现出较好的一致性,热脉冲法能够用于新疆杨蒸腾测定;液流—株间微型蒸渗仪法和水量平衡法测算结果的相对误差在±15%之内,液流—株间微型蒸渗仪法能够适用于新疆杨蒸发蒸腾量测定。 展开更多
关键词 新疆杨 蒸发蒸腾量 热脉冲法 液流-株间微型蒸渗仪法 水量平衡法 适用性 populus alba var
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转果聚糖蔗糖转移酶基因银腺杨的获得(英文) 被引量:21
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作者 张冰玉 苏晓华 +2 位作者 黄秦军 张香华 胡赞民 《林业科学》 EI CAS CSCD 北大核心 2005年第3期48-53,共6页
采用农杆菌介导的遗传转化方法,将来自枯草杆菌的果聚糖蔗糖转移酶基因(SacB)导入银腺杨,以提高杨树对水分胁迫的抗性。以来自无菌培养的叶片为外植体,通过大约10 0 0个叶盘与农杆菌LBA4 4 0 4共培养,将植物双元表达载体pKP中SacB基因... 采用农杆菌介导的遗传转化方法,将来自枯草杆菌的果聚糖蔗糖转移酶基因(SacB)导入银腺杨,以提高杨树对水分胁迫的抗性。以来自无菌培养的叶片为外植体,通过大约10 0 0个叶盘与农杆菌LBA4 4 0 4共培养,将植物双元表达载体pKP中SacB基因导入银腺杨基因组,经卡那霉素筛选后,共获得10 2株卡那霉素抗性植株。经PCR特异性扩增和Southern点杂交分析,证明其中97株再生植株基因组DNA中整合了SacB基因。对其中的6 2个无性系进行RT PCR分析,结果表明SacB基因在其中的5 0个无性系中获得表达。温室生长观察表明,转基因无性系外部形态与对照相比没有稳定的显著差异,少数部分转基因无性系的生长明显受到抑制,其他转基因无性系生长正常。这些转基因无性系的获得为培育抗旱转基因杨树奠定了基础。 展开更多
关键词 银腺杨 果聚糖蔗糖转移酶基因 转基因 农杆菌介导
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外源SacB基因在银腺杂种杨基因组中的表达及抗旱性分析 被引量:13
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作者 李义良 苏晓华 +1 位作者 张冰玉 张志毅 《北京林业大学学报》 CAS CSCD 北大核心 2007年第2期1-6,共6页
利用转基因技术培育抗旱杨树新品种是改善干旱地区生态环境的有效途径之一.该研究对已整合外源SacB基因的银腺杂种杨株系进行半定量RT-PCR和温室水分胁迫试验.研究结果表明,在所测定的7个转基因株系中,外源SacB基因均成功转录mRNA;转基... 利用转基因技术培育抗旱杨树新品种是改善干旱地区生态环境的有效途径之一.该研究对已整合外源SacB基因的银腺杂种杨株系进行半定量RT-PCR和温室水分胁迫试验.研究结果表明,在所测定的7个转基因株系中,外源SacB基因均成功转录mRNA;转基因株系叶片中积累了SacB基因表达产物(果聚糖);在干旱胁迫条件下,转基因株系的生长量、生物量和叶片含水量明显高于对照.相关分析表明转基因株系生长量、生物量和叶片含水量与果聚糖积累量呈极显著正相关,说明SacB基因的导入提高了转基因杨树对水分胁迫的抗性. 展开更多
关键词 SAC B基因 银腺杂种杨 转基因 抗旱性
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新疆杨高效遗传转化系统的建立 被引量:19
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作者 诸葛强 王婕琛 +2 位作者 陈英 黄敏仁 王明庥 《植物资源与环境学报》 CAS CSCD 2003年第4期6-10,共5页
选择新疆杨(PopulusalbaL.var.pyramidalisBge.)为遗传转化受体材料,为建立根癌农杆菌介导新疆杨高效遗传转化系统,从预培养时间、侵染时间、共培养时间、添加乙酰丁香酮(AS)的时机、共培养培养基中添加乙酰丁香酮浓度、侵染菌液的制备... 选择新疆杨(PopulusalbaL.var.pyramidalisBge.)为遗传转化受体材料,为建立根癌农杆菌介导新疆杨高效遗传转化系统,从预培养时间、侵染时间、共培养时间、添加乙酰丁香酮(AS)的时机、共培养培养基中添加乙酰丁香酮浓度、侵染菌液的制备方法、外植体继代方式等7个方面优化筛选。结果显示较合适的转化系统为:预培养8h,农杆菌菌液(OD600=0.4)侵染15min,共培养5d,侵染菌液的最优制备方法是液体培养活化农杆菌2次加离心收集菌体重悬,共培养培养基中添加乙酰丁香酮80μmol/L。新疆杨叶盘转化频率可达38.10%。 展开更多
关键词 新疆杨 遗传转化系统 根癌农杆菌 农杆菌
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转基因银腺杂种杨对土壤微生物的影响 被引量:13
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作者 侯英杰 苏晓华 +2 位作者 焦如珍 黄秦军 褚延广 《林业科学》 EI CAS CSCD 北大核心 2009年第5期148-152,共5页
In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples f... In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms. 展开更多
关键词 转基因银腺杂种杨 土壤微生物 生态安全
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新疆杨叶肉原生质体游离和纯化的研究 被引量:19
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作者 景艳春 康向阳 +1 位作者 王君 李代丽 《西北植物学报》 CAS CSCD 北大核心 2007年第3期509-514,共6页
以新疆杨(Populus alba L.var.pyramidalis)无菌苗叶片为材料,就其原生质体游离、纯化方法及影响因素等进行了研究.结果表明,cellulase R-10、hemicellulase和pectolase Y-23对原生质体产量有极显著影响.适宜新疆杨叶片原生质体游离... 以新疆杨(Populus alba L.var.pyramidalis)无菌苗叶片为材料,就其原生质体游离、纯化方法及影响因素等进行了研究.结果表明,cellulase R-10、hemicellulase和pectolase Y-23对原生质体产量有极显著影响.适宜新疆杨叶片原生质体游离的条件为CPW+KM8P+3.0%cellulase R-10+0.5%~1.5% macerozyme R-10+0.5%hemicellulase+0.1%~0.5% pectolase Y-23+0.6 mol/L甘露醇,酶解温度27℃,酶解时间8 h.在此条件下,原生质体产量达1.57×107 个/gFW,活力79.41%,而且蔗糖等密度离心法中的上浮法纯化原生质体效果最佳,最适蔗糖浓度为30%. 展开更多
关键词 新疆杨 原生质体 游离 纯化
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豇豆胰蛋白酶抑制剂 (CpTI)抗虫转基因新疆杨的获得 被引量:16
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作者 诸葛强 王婕琛 +4 位作者 陈英 伍宁丰 黄敏仁 范云六 王明庥 《分子植物育种》 CAS CSCD 2003年第4期491-496,共6页
利用根癌农杆菌介导法将广谱抗虫基因豇豆胰蛋白酶抑制剂 (CpTI)基因导入新疆杨 (Populusal bavar pyramidalis) ,经筛选获得了大量转基因植株。对转基因植株经PCR鉴定、PCR Southern和点杂交分子检测结果证实CpTI基因已整合进杨树基因... 利用根癌农杆菌介导法将广谱抗虫基因豇豆胰蛋白酶抑制剂 (CpTI)基因导入新疆杨 (Populusal bavar pyramidalis) ,经筛选获得了大量转基因植株。对转基因植株经PCR鉴定、PCR Southern和点杂交分子检测结果证实CpTI基因已整合进杨树基因组中。部分转基因植株的饲虫实验表明 。 展开更多
关键词 豇豆胰蛋白酶抑制剂 抗虫基因 新疆杨 转基因 根癌农杆菌 CPTI基因
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银腺杨与毛新杨正反交三倍体选育 被引量:26
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作者 康向阳 朱之悌 张志毅 《北京林业大学学报》 EI CAS CSCD 北大核心 2000年第6期8-11,共4页
采用花粉染色体人工加倍获得的银腺杨 (Populusalba×P .glandulosa)、毛新杨 (P .tomentosa×P .bolleana)未减数 2n花粉 ,施加一定剂量的辐射处理后杂交 ,两杂交组合共获得 16株三倍体 .其中银腺杨×毛新杨组合以1470rad... 采用花粉染色体人工加倍获得的银腺杨 (Populusalba×P .glandulosa)、毛新杨 (P .tomentosa×P .bolleana)未减数 2n花粉 ,施加一定剂量的辐射处理后杂交 ,两杂交组合共获得 16株三倍体 .其中银腺杨×毛新杨组合以1470rad处理最佳 ,三倍体得率 3 .8% ;而毛新杨×银腺杨 (16 80rad)则获得了高达 12 .9%的三倍体 .三倍体整体生长水平优势明显 ,单株生长更为突出 ,2年生实生苗的苗高和地径最高 4.9m和 5 .8cm ,分别超出群体平均值的5 8%和 176 % ;并且正反交三倍体的遗传效应差异明显 .利用未减数 2n花粉 ,经花粉辐射处理杂交选育杂种三倍体 ,将是今后白杨乃至杨属植物育种的最有效途径之一 . 展开更多
关键词 银腺杨 毛新杨 2N花粉 花粉辐射 三倍体 选育
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新疆杨不同混交模式对天牛危害和林木生长的影响 被引量:9
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作者 王涛 温俊宝 +4 位作者 许志春 骆有庆 宗世祥 曹川健 宝山 《林业科学研究》 CSCD 北大核心 2006年第4期504-508,共5页
为验证多树种合理配置抗御杨树天牛灾害的效果,在宁夏新华桥种苗场的试验林内,选取受光肩星天牛危害的3种配置模式的林分为样地,通过对新疆杨标准木做树干解析。比较3种配置模式中新疆杨的单株总生长量,结果除胸径外,树高和材积的总生... 为验证多树种合理配置抗御杨树天牛灾害的效果,在宁夏新华桥种苗场的试验林内,选取受光肩星天牛危害的3种配置模式的林分为样地,通过对新疆杨标准木做树干解析。比较3种配置模式中新疆杨的单株总生长量,结果除胸径外,树高和材积的总生长量均存在显著差异;单株材积总生长量大小顺序为新疆杨和复叶槭混交林>新疆杨和合作杨混交林>新疆杨纯林,即混交林中天牛对新疆杨危害不同程度地小于新疆杨纯林。比较3种模式林分在单位面积上的总材积生长量,结果为新疆杨和复叶槭混交林>新疆杨纯林>新疆杨和合作杨的混交林。同时考虑目标树种的单株材积生长量和该配置模式单位面积上的总材积生长量,新疆杨和复叶槭混交林是3种模式中最理想的。 展开更多
关键词 光肩星天牛 配置模式 新疆杨 生长量
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一株能拮抗3种土传病害病原真菌的长枝木霉 被引量:17
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作者 常媛 杨兴堂 +4 位作者 姜传英 姚志红 贾让 任龙辉 张荣沭 《草业科学》 CAS CSCD 北大核心 2017年第2期246-254,共9页
为了丰富生防木霉菌(Trichodermaspp.)种资源,在园林植物八宝景天(Sedum spectabile)根际土壤中分离得到一株优势木霉。根据该菌株菌落的形态特征、形态学显微观察、菌株rDNA-ITS序列分析以及邻接法同源性比对等结果判定木霉菌株种类。... 为了丰富生防木霉菌(Trichodermaspp.)种资源,在园林植物八宝景天(Sedum spectabile)根际土壤中分离得到一株优势木霉。根据该菌株菌落的形态特征、形态学显微观察、菌株rDNA-ITS序列分析以及邻接法同源性比对等结果判定木霉菌株种类。采用平板对峙法将该菌株与3种土传病害病原菌[(Sclerotinia sclerotiorum)、立枯丝核菌(Rhizoctonia solani)和细链格孢菌(Alternaria alternata)]进行对峙培养。并用该木霉诱导山新杨(Populus davidiana×P.albavar.Pyramidalis)组培苗,观察离体叶片抵抗细链格孢菌侵染的能力。结果表明,此木霉为长枝木霉(Trichoderma longibrachiatum),命名为Tl-70。该菌株对3种土传病害病原真菌均有较明显的拮抗作用。对核盘菌的抑菌率最高为77.71%,显著高于对细链格孢菌和立枯丝核菌的抑制率(P<0.05);对立枯丝核菌的抑菌率为58.56%,显著高于对细链格孢菌的抑制率(P<0.05);对细链格孢菌的抑菌率最低,为53.32%。并且,该木霉能提高山新杨组培苗叶片拮抗细链格孢菌侵染的能力。说明此菌株是具有潜力的生防菌株。 展开更多
关键词 木霉菌 形态特征 ITS序列分析 系统发育树 拮抗作用 山新杨
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转vgb基因银腺杂种杨的耐涝性 被引量:6
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作者 李义良 苏晓华 +2 位作者 张冰玉 黄秦军 张香华 《林业科学》 EI CAS CSCD 北大核心 2009年第7期26-31,共6页
对导入透明颤菌血红蛋白(vitreoscilla hemoglobin,VHb)基因vgb银腺杂种杨进行Southern印记杂交和RT-PCR检测,证实vgb基因已整合到杨树基因组中,并在转录水平上得到表达。在温室对4个转基因株系及对照盆栽扦插苗进行模拟持续淹水胁迫试... 对导入透明颤菌血红蛋白(vitreoscilla hemoglobin,VHb)基因vgb银腺杂种杨进行Southern印记杂交和RT-PCR检测,证实vgb基因已整合到杨树基因组中,并在转录水平上得到表达。在温室对4个转基因株系及对照盆栽扦插苗进行模拟持续淹水胁迫试验,研究淹水条件下植株的生长及生理性状。结果表明,随着淹水时间的延长苗木逐渐死亡,对照植株在淹水17天后死亡率达66.67%,而4个转基因株系死亡率均低于50.00%,其中T81最低,只有25.00%;同时4个转基因株系在淹水胁迫时能够保持较高的叶绿素含量,并且膜质过氧化物MDA含量均低于对照植株;涝渍胁迫下转基因株系生长受抑制程度低于对照植株。说明在淹水胁迫下vgb基因的表达增强了银腺杂种杨叶绿素含量的积累,进而促进了转基因植株的生长,提高了转基因株系对淹水的忍受能力。 展开更多
关键词 透明颤菌血红蛋白基因(vgb) 耐涝性 银腺杂种杨
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