The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown ...The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.展开更多
Development of transgenic plants with tolerance to environ- mental stress is an important goal of plant biotechnology. （LEA） proteins accumulate in seeds dur- ing late embryogenesis, where they protect cellular memb...Development of transgenic plants with tolerance to environ- mental stress is an important goal of plant biotechnology. （LEA） proteins accumulate in seeds dur- ing late embryogenesis, where they protect cellular membranes and macromolecules against drought. In this work, we transferred the Tamarix androssowii LEA gene into hybrids of Populus davidiana xp. bolleana. We compared relative rates of height growth, chlorophyll fluo- rescence kinetic parameters, and leaf Na＋ levels of six TaLEA-containing lines with non-transferred plants （NT）, all grown under 0.8% NaC1 stress condition. Survival percentages of transgenic lines were all higher than for NT controls after rehydration and the sur- vival percentage of SL2 was five-fold higher than for NT controls. Seed- ling height increased 48.7% in SL2 （from the onset of induced stress to the end of the growing season）, 31% more than for the NT controls. Chlorophyll fluorescence kinetic parameters showed a marked increase in photosynthetic capacity in SL2 and SL5. Na＋ levels in young leaves of transgenic lines were lower than in control NT leaves, but higher in yel- low and withered leaves, indicating improved salt tolerance in transgenic lines.展开更多
Soil respiration from decomposing aboveground litter is a major component of the terrestrial carbon cycle. However, variations in the contribution of aboveground litter to the total soil respiration for stands of vary...Soil respiration from decomposing aboveground litter is a major component of the terrestrial carbon cycle. However, variations in the contribution of aboveground litter to the total soil respiration for stands of varying ages are poorly understood. To assess soil respiration induced by aboveground litter, treatments of litter and no litter were applied to 5-, l0-, and 20-year-old stands of Populus davidiana Dode in the sandstorm source area of Beijing-Tianjin, equations were applied to China. Optimal nonlinear model the combined effects of soil temperature and soil water content on soil respiration. Results showed that the monthly average contribution of aboveground litter to total soil respiration were 18.46% ± 4.63%, 16.64% ± 9.31%, and 22.37% ± 8.17% for 5-, 10-, and ao-year-old stands, respectively. The relatively high contribution in 5- and 20-year-old stands could be attributed to easily decomposition products and high accumulated litter, resoectivelv. Also. it fluctuated monthly for all stand ages due to substrate availability caused by phenology and environmental factors. Litter removal significantly decreased soil respiration and soil water content for all stand ages （P 〈 0.05） but not soil temperature （P 〉 0.05）. Variations of soil respiration could be explained by soil temperature at 5-cm depth using an exponential equation and by soil water content at lo-cm depth using a quadratic equation, whereas soil respiration was better modeled using the combined parameters of soil temperature and soil water content than with either soil temperature or soil water content alone. Temperature sensitivity （Q10） increased with stand age in both the litter and the no litter treatments. Considering the effects of aboveground litter, this study provides insights for predicting future soil carbon fluxes and for accurately assessing soil carbon budgets.展开更多
Populus bolleana is a variety of P. alba, commonly used in poplar breeding programs in China. Developmental biology that involves staminate flowers, microsporogenesis and microgametogenesis ofP. bolleana is essential ...Populus bolleana is a variety of P. alba, commonly used in poplar breeding programs in China. Developmental biology that involves staminate flowers, microsporogenesis and microgametogenesis ofP. bolleana is essential for Populus improvement in cross breeding for better characteristics in sexual reproduction. Flower morphology and pollen development were described and illustrated using anatomical, sectioning and stain-clearing techniques. The results show that microsporocytes undergo a regular meiotic process, but some multi-nucleate microspores occur at the microspore stage. It takes five days for microsporocytes to develop to mature pollen by forcing flower branches under greenhouse conditions. Additionally, an important relationship was found between stages of meiosis and anther colors. Microspore tetrads formed when the anther color turned yellow, whereas, when the pollen matured, the anther was red and the tapetum degenerated completely. When mature pollen grains are formed, flower buds develop into male catkins. In the end, filament elongated and pollen grains were released from dehisced anthers.展开更多
The root segments selected from dominant trees of Populus davidiana Dode were taken as reproductive material and were buried in different depths to carry out the reproduction of root turion sprout. The affecting facto...The root segments selected from dominant trees of Populus davidiana Dode were taken as reproductive material and were buried in different depths to carry out the reproduction of root turion sprout. The affecting factors of germination rate, survival rate, and height growth for cutting wood of root sprout were comparatively analyzed. The results showed that the best suitable substrate for burying root is pearlite, with a germination rate of 15.16%. 3-4-cm root segments has the highest rate of germination (12.4%). The mixture of sand and soil (2:1) is the best cutting substrate, with a survival rate of 81.3%, while as to height growth of cutting wood, the mixture of turfy and soil (1:1) is the best. The cutting woods selected from different positions of stem show difference in height growth. The cutting wood from top stem is higher obviously than those from middle or low stem.展开更多
The effect of water state of soil on eco-boundary over leaf surface was disscussed by takingPopulus davidiana as experimental material. The results show that to change soil water state by some methods was to adjust an...The effect of water state of soil on eco-boundary over leaf surface was disscussed by takingPopulus davidiana as experimental material. The results show that to change soil water state by some methods was to adjust and control the forming quality of eco-boundary in fact. The plant in good water condition must be accompanied by thinner eco-boundary. This eco-boundary is beneficial to exchange and flow of the substances between plant and environment.展开更多
A new diterpenoid, named populusol A （1）, was isolated from the methanolic extraction of the stem bark of Populus davidiana. The structure was elucidated on the basis of extensive 1D and 2D NMR as well as HRFAB-MS s...A new diterpenoid, named populusol A （1）, was isolated from the methanolic extraction of the stem bark of Populus davidiana. The structure was elucidated on the basis of extensive 1D and 2D NMR as well as HRFAB-MS spectroscopic analysis.展开更多
Phytochemical study on the BuOH-soluble fraction of the stem bark of Populus davidiana resulted in the isolation of a new salicin derivative （1）, named davidianoside. The structure was elucidated on the basis of ext...Phytochemical study on the BuOH-soluble fraction of the stem bark of Populus davidiana resulted in the isolation of a new salicin derivative （1）, named davidianoside. The structure was elucidated on the basis of extensive physicochemical and spectroscopic analyses.展开更多
The rough extent of leaf surface may be described by protruding of waxes which like as wart and of stomatal aperture and of width of the cuticular ledge around guard cells. Because the morphology of the leaf surface h...The rough extent of leaf surface may be described by protruding of waxes which like as wart and of stomatal aperture and of width of the cuticular ledge around guard cells. Because the morphology of the leaf surface has obviously similar itseIf, so one can use the theory of the fractal dimension to deal with the problems of leaf surface rough. The paper studied the rough extent and the result showed: with the leaf growing up, the waxes accumulate more and more, the leaf surface is more rough and the dimension is larger. The dimension D which indicates the rough extent of warts protruding is about 2. 10-2.20 on the above epidermis; On the beneath epidermis the dimension D of stomatal opening protruding is about 2.00-2.24, Maximum width L of cuticular ledge is about 1.0-7.4μm.展开更多
基金part of the Liaoning Provincial Department of Education project LJKZ0684supported by the National Natural Science Foundation of China (31800542)
文摘The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.
基金supported by the National High-Tech Research and Development Program of China,863 Program(Molecular Breeding and New Species Creating of Poplar)(Grant No.2011AA100201)
文摘Development of transgenic plants with tolerance to environ- mental stress is an important goal of plant biotechnology. （LEA） proteins accumulate in seeds dur- ing late embryogenesis, where they protect cellular membranes and macromolecules against drought. In this work, we transferred the Tamarix androssowii LEA gene into hybrids of Populus davidiana xp. bolleana. We compared relative rates of height growth, chlorophyll fluo- rescence kinetic parameters, and leaf Na＋ levels of six TaLEA-containing lines with non-transferred plants （NT）, all grown under 0.8% NaC1 stress condition. Survival percentages of transgenic lines were all higher than for NT controls after rehydration and the sur- vival percentage of SL2 was five-fold higher than for NT controls. Seed- ling height increased 48.7% in SL2 （from the onset of induced stress to the end of the growing season）, 31% more than for the NT controls. Chlorophyll fluorescence kinetic parameters showed a marked increase in photosynthetic capacity in SL2 and SL5. Na＋ levels in young leaves of transgenic lines were lower than in control NT leaves, but higher in yel- low and withered leaves, indicating improved salt tolerance in transgenic lines.
基金funded by the National Natural Science Foundation of China (Grant No.31170414)the 100 Talents Program of Chinese Academy of Sciences,and the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No.XDA05060600)
文摘Soil respiration from decomposing aboveground litter is a major component of the terrestrial carbon cycle. However, variations in the contribution of aboveground litter to the total soil respiration for stands of varying ages are poorly understood. To assess soil respiration induced by aboveground litter, treatments of litter and no litter were applied to 5-, l0-, and 20-year-old stands of Populus davidiana Dode in the sandstorm source area of Beijing-Tianjin, equations were applied to China. Optimal nonlinear model the combined effects of soil temperature and soil water content on soil respiration. Results showed that the monthly average contribution of aboveground litter to total soil respiration were 18.46% ± 4.63%, 16.64% ± 9.31%, and 22.37% ± 8.17% for 5-, 10-, and ao-year-old stands, respectively. The relatively high contribution in 5- and 20-year-old stands could be attributed to easily decomposition products and high accumulated litter, resoectivelv. Also. it fluctuated monthly for all stand ages due to substrate availability caused by phenology and environmental factors. Litter removal significantly decreased soil respiration and soil water content for all stand ages （P 〈 0.05） but not soil temperature （P 〉 0.05）. Variations of soil respiration could be explained by soil temperature at 5-cm depth using an exponential equation and by soil water content at lo-cm depth using a quadratic equation, whereas soil respiration was better modeled using the combined parameters of soil temperature and soil water content than with either soil temperature or soil water content alone. Temperature sensitivity （Q10） increased with stand age in both the litter and the no litter treatments. Considering the effects of aboveground litter, this study provides insights for predicting future soil carbon fluxes and for accurately assessing soil carbon budgets.
基金supported by the National Natural Science Foundation of China(Grant No.30471407)the Graduate Student Foundation of Beijing Forestry University(No.0@008).
文摘Populus bolleana is a variety of P. alba, commonly used in poplar breeding programs in China. Developmental biology that involves staminate flowers, microsporogenesis and microgametogenesis ofP. bolleana is essential for Populus improvement in cross breeding for better characteristics in sexual reproduction. Flower morphology and pollen development were described and illustrated using anatomical, sectioning and stain-clearing techniques. The results show that microsporocytes undergo a regular meiotic process, but some multi-nucleate microspores occur at the microspore stage. It takes five days for microsporocytes to develop to mature pollen by forcing flower branches under greenhouse conditions. Additionally, an important relationship was found between stages of meiosis and anther colors. Microspore tetrads formed when the anther color turned yellow, whereas, when the pollen matured, the anther was red and the tapetum degenerated completely. When mature pollen grains are formed, flower buds develop into male catkins. In the end, filament elongated and pollen grains were released from dehisced anthers.
文摘The root segments selected from dominant trees of Populus davidiana Dode were taken as reproductive material and were buried in different depths to carry out the reproduction of root turion sprout. The affecting factors of germination rate, survival rate, and height growth for cutting wood of root sprout were comparatively analyzed. The results showed that the best suitable substrate for burying root is pearlite, with a germination rate of 15.16%. 3-4-cm root segments has the highest rate of germination (12.4%). The mixture of sand and soil (2:1) is the best cutting substrate, with a survival rate of 81.3%, while as to height growth of cutting wood, the mixture of turfy and soil (1:1) is the best. The cutting woods selected from different positions of stem show difference in height growth. The cutting wood from top stem is higher obviously than those from middle or low stem.
文摘The effect of water state of soil on eco-boundary over leaf surface was disscussed by takingPopulus davidiana as experimental material. The results show that to change soil water state by some methods was to adjust and control the forming quality of eco-boundary in fact. The plant in good water condition must be accompanied by thinner eco-boundary. This eco-boundary is beneficial to exchange and flow of the substances between plant and environment.
基金a grant of the Biogreen 21 program(2006),Rural Development Administration,Republic of Koreathe Korea Basic Science Institute(KBSI)for supplying the NMR spectra.
文摘A new diterpenoid, named populusol A （1）, was isolated from the methanolic extraction of the stem bark of Populus davidiana. The structure was elucidated on the basis of extensive 1D and 2D NMR as well as HRFAB-MS spectroscopic analysis.
基金supported by grants of Biogreen 21 project(2006,Rural Development Administration,Republic of Korea)and Scientific research development fund of Zhejiang Forestry University(2007)
文摘Phytochemical study on the BuOH-soluble fraction of the stem bark of Populus davidiana resulted in the isolation of a new salicin derivative （1）, named davidianoside. The structure was elucidated on the basis of extensive physicochemical and spectroscopic analyses.
文摘The rough extent of leaf surface may be described by protruding of waxes which like as wart and of stomatal aperture and of width of the cuticular ledge around guard cells. Because the morphology of the leaf surface has obviously similar itseIf, so one can use the theory of the fractal dimension to deal with the problems of leaf surface rough. The paper studied the rough extent and the result showed: with the leaf growing up, the waxes accumulate more and more, the leaf surface is more rough and the dimension is larger. The dimension D which indicates the rough extent of warts protruding is about 2. 10-2.20 on the above epidermis; On the beneath epidermis the dimension D of stomatal opening protruding is about 2.00-2.24, Maximum width L of cuticular ledge is about 1.0-7.4μm.
