Using complete and orthogonal design, fertilization trials were conducted for the young growth of Populus deltoids cv. Zhonghua hongye in the fluviogenic soil in Xindu District, Chengdu City. The results showed that t...Using complete and orthogonal design, fertilization trials were conducted for the young growth of Populus deltoids cv. Zhonghua hongye in the fluviogenic soil in Xindu District, Chengdu City. The results showed that the application ef- fect of the single nutrient elements ranked as K〉N〉P, and that of the fertilization combinations ranked as N＋P＋K〉N＋K〉N＋P, P＋K. The optimum fertilization formula was urea [（NH2）2CO, 150 g/plant] ＋ calcium superphosphate [Ca（H2PO4）2.H20, 220 g/plant] ＋ potassium sulphate （K2SO4, 75 g/plant）.展开更多
The new emerging stems and buds of poplar 110(Populus deltoids×P.maximowiczii cv ’Eridano’) were used as explants and incubated on MS media supplemented with various hormone combinations.Once the successive and...The new emerging stems and buds of poplar 110(Populus deltoids×P.maximowiczii cv ’Eridano’) were used as explants and incubated on MS media supplemented with various hormone combinations.Once the successive and rooting plants were obtained through selection of cultural medium formula,leaves and internodals were excised from tubed-culture planlets and induced to form regenerated plants.The result showed MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+TDZ 0.02 mg·L-1 was beneficial for leaves to inducing adventitious buds;The proper medium for using internodals as explants was MS+6-BA 0.3 mg·L-1+NAA 0.05 mg·L-1+TDZ 0.02 mg·L-1;while the medium of MS+6-BA 0.3 mg·L-1+NAA 0.1 mg·L-1 was proper to culturing the stronger tubed-planlets.The optimum medium for producing roots was 1/2MS+IBA 0.5 mg·L-1.The effect of different incising mode and growth state of leaves and internodals on inducing adventitious was discussed.In vitro vegetative propagation and plant rooting were optimized to establish the high-efficient tissue culture reproduction system of poplar 110,which make it possible for tree breeding and genetic transformation of this species.展开更多
基金Supported by Special Scientific Research Fund of Forestry Public Welfare Profession of China(201104026)~~
文摘Using complete and orthogonal design, fertilization trials were conducted for the young growth of Populus deltoids cv. Zhonghua hongye in the fluviogenic soil in Xindu District, Chengdu City. The results showed that the application ef- fect of the single nutrient elements ranked as K〉N〉P, and that of the fertilization combinations ranked as N＋P＋K〉N＋K〉N＋P, P＋K. The optimum fertilization formula was urea [（NH2）2CO, 150 g/plant] ＋ calcium superphosphate [Ca（H2PO4）2.H20, 220 g/plant] ＋ potassium sulphate （K2SO4, 75 g/plant）.
文摘The new emerging stems and buds of poplar 110(Populus deltoids×P.maximowiczii cv ’Eridano’) were used as explants and incubated on MS media supplemented with various hormone combinations.Once the successive and rooting plants were obtained through selection of cultural medium formula,leaves and internodals were excised from tubed-culture planlets and induced to form regenerated plants.The result showed MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+TDZ 0.02 mg·L-1 was beneficial for leaves to inducing adventitious buds;The proper medium for using internodals as explants was MS+6-BA 0.3 mg·L-1+NAA 0.05 mg·L-1+TDZ 0.02 mg·L-1;while the medium of MS+6-BA 0.3 mg·L-1+NAA 0.1 mg·L-1 was proper to culturing the stronger tubed-planlets.The optimum medium for producing roots was 1/2MS+IBA 0.5 mg·L-1.The effect of different incising mode and growth state of leaves and internodals on inducing adventitious was discussed.In vitro vegetative propagation and plant rooting were optimized to establish the high-efficient tissue culture reproduction system of poplar 110,which make it possible for tree breeding and genetic transformation of this species.
文摘转基因技术是当今林木分子育种的手段之一,但其生物安全性问题广受关注。本研究选择进入田间试验的转PeTLP(类甜蛋白)基因‘南林895’杨Populus deltoides×P. euramericana cv.‘Nanlin 895’为材料,开展转基因杨树对土壤微生物影响及外源基因分子检测等分析。结果显示,转基因杨树在进入田间试验一年后,外源基因仍稳定存在于转基因植株基因组中;转基因与非转基因植株根系周围可培养土壤微生物菌落数量无显著差异,表明外源基因对土壤微生物无显著影响;对土壤微生物总DNA进行分子检测也显示外源基因未有向周围土壤微生物扩散现象;叶片化感作用试验显示转基因植株叶片未对生菜Lactuca sativa L. var. ramose Hort.种子的生长造成显著影响。初步分析结果表明,转PeTLP基因‘南林895’杨在进入田间试验后未出现外源基因水平转移,也未对周围生态环境造成显著影响。
