The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using th...The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized PsneIFSA2 and PsneIFSA4 from young poplar （P. simonii × P. nigra） leaves. The deduced amino acid sequences of PsneIF5A2 and PsneIF5A4 were 98 % similar to each other, and they are orthologs of eIF5A 1 in Arabidopsis. In a subcellular localization analysis, PsneIF5A2 and PsneIF5A4 proteins were localized in the nucleus and cytoplasm, qRT-PCR analysis showed that PsneIF5A2 and PsneIF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing PsneIF5A2 and PsneIF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that PsneIF5A2 and PsneIF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants.展开更多
[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating t...[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar.展开更多
Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.py...Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.pyramidalis ＇Opera 8277＇ cuttings were examined by using a gas chromatography/mass spectrometry（GC/MS） technique at three levels of light intensity（400,800 and 1 200 μmol·m-2·s-1）.A positive correlation between the emissions of these aldehydes and light intensity was found.Moreover,nordi-hydroguaiaretic acid（NDGA）,a special inhibitor of lipoxygenase（LOX）,significantly inhibited the emissions of C6-C9 aldehydes at three levels of light intensity,but did not influence the emission of decanal（C10）.The emissions of C6-C10 aldehydes in NDGA treated poplar cuttings,exhibited the same positive correlation with light intensity.The results indicated that LOX pathway contributes to the emissions of C6-C9 aldehydes,whereas some pathways regulated by light intensity might be a universal mechanism for emissions of C6-C10 aldehydes.展开更多
A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase （LOX）, a key enzyme ofjasmonate （JA） pathway, and phenylalanine ammonia lya...A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase （LOX）, a key enzyme ofjasmonate （JA） pathway, and phenylalanine ammonia lyase （PAL）, a rate-limiting enzyme of phenyl- propanoid pathway, were measured in the leaves of one-year-old poplar （Populus simonii × P. pyramidalis ＇Opera 8277＇） cuttings after Clostera anachoreta larvae attack. The results show that the increased activities of LOX and PAL were found not only in the leaves wounded by C. anachoreta larvae but also in their tipper systemic leaves, indicating that JA and phenylpropanoid pathways were activated, and the defense response was stimulated systemically. The increase in LOX and PAL activities in neighboring intact poplar cuttings sug- gested that there exists the interplant communication between poplar plants mediated by the herbivore-induced volatiles. Methyl jasmonate （MeJA） was also proved to be an airborne signal to induce defense response in P simonii × P pyramidalis ＇Opera 8277＇ cuttings.展开更多
The aim of this study was to investigate the role of rhizosphere organic phosphorus （P） in soil P supply in semiarid forests and the effects of tree species on rihizosphere organic P. We examined organic P fractions...The aim of this study was to investigate the role of rhizosphere organic phosphorus （P） in soil P supply in semiarid forests and the effects of tree species on rihizosphere organic P. We examined organic P fractions in rhizosphere and bulk soils of mono-specific Simon poplar （Populus simonii） and Mongolian pine （Pinus sylvestris var. mongolica） plantations in a semiarid sandy soil of Horqin Sandy Land in Northeast China. Total organic P （TPo） accounted for 76% of total P across the two stands. The concentration of organic P （Po） fractions decreased in the order of NaOH-Po〉Res-Po〉HCI-Po〉NaHCO3-Po in both plantations. The concentration of NaHCO3-Po was 38% and 43% lower in rhizosphere soil than in bulk soil in Simon poplar and Mongolian pine plantations, respectively. In contrast, total P, TPo and NaOH-Po significantly accumulated in rhizosphere soil in Simon poplar plantations, but no change in Mongolian pine plantations. Soil recalcitrant organic P fractions were positively correlated with soil organic carbon. The results suggest that rhizosphere labile organic P was an important source of plant-available P in this semiarid region, but the dynamic of rhizosphere recalcitrant organic P fractions varied with tree species and was correlated to organic carbon dynamics.展开更多
基金supported by the Program for New Century Excellent Talents in University(No.NCET-12-0808)National Natural Science Foundation of China(No.31370661)the Fundamental Research Funds for the Central Universities(No.2572014AA26)
文摘The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized PsneIFSA2 and PsneIFSA4 from young poplar （P. simonii × P. nigra） leaves. The deduced amino acid sequences of PsneIF5A2 and PsneIF5A4 were 98 % similar to each other, and they are orthologs of eIF5A 1 in Arabidopsis. In a subcellular localization analysis, PsneIF5A2 and PsneIF5A4 proteins were localized in the nucleus and cytoplasm, qRT-PCR analysis showed that PsneIF5A2 and PsneIF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing PsneIF5A2 and PsneIF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that PsneIF5A2 and PsneIF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants.
基金Supported by National Natural Science Foundation of China(31370661)
文摘[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar.
基金supported by National Natural Science Foundation of China (30871727 31071817)+1 种基金the Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality (PXM20090142076309)the Beijing Science and Technology Plan Program (Z080005032508017)
文摘Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.pyramidalis ＇Opera 8277＇ cuttings were examined by using a gas chromatography/mass spectrometry（GC/MS） technique at three levels of light intensity（400,800 and 1 200 μmol·m-2·s-1）.A positive correlation between the emissions of these aldehydes and light intensity was found.Moreover,nordi-hydroguaiaretic acid（NDGA）,a special inhibitor of lipoxygenase（LOX）,significantly inhibited the emissions of C6-C9 aldehydes at three levels of light intensity,but did not influence the emission of decanal（C10）.The emissions of C6-C10 aldehydes in NDGA treated poplar cuttings,exhibited the same positive correlation with light intensity.The results indicated that LOX pathway contributes to the emissions of C6-C9 aldehydes,whereas some pathways regulated by light intensity might be a universal mechanism for emissions of C6-C10 aldehydes.
基金supported by the Pro-gramme for Changjiang Scholars and the Innovative Research Team in Universities of China (PCSIRT0607)by the National Natural Science Foundation of China (30871727+2 种基金 30872037)the National Key Project of Scientific and Technical Supporting Programmes Funded by the Ministry of Science & Technology of China (2006BAD01A15 2006BAD24B04)
文摘A study was conducted to explore the defense response in woody plants after insect herbivory. The activities of two enzymes, lipoxygenase （LOX）, a key enzyme ofjasmonate （JA） pathway, and phenylalanine ammonia lyase （PAL）, a rate-limiting enzyme of phenyl- propanoid pathway, were measured in the leaves of one-year-old poplar （Populus simonii × P. pyramidalis ＇Opera 8277＇） cuttings after Clostera anachoreta larvae attack. The results show that the increased activities of LOX and PAL were found not only in the leaves wounded by C. anachoreta larvae but also in their tipper systemic leaves, indicating that JA and phenylpropanoid pathways were activated, and the defense response was stimulated systemically. The increase in LOX and PAL activities in neighboring intact poplar cuttings sug- gested that there exists the interplant communication between poplar plants mediated by the herbivore-induced volatiles. Methyl jasmonate （MeJA） was also proved to be an airborne signal to induce defense response in P simonii × P pyramidalis ＇Opera 8277＇ cuttings.
基金funded by the National Natural Science Foundation of China (41373087, 30800887)the State Key Laboratory of Forest and Soil Ecology (LFSE2013-11)
文摘The aim of this study was to investigate the role of rhizosphere organic phosphorus （P） in soil P supply in semiarid forests and the effects of tree species on rihizosphere organic P. We examined organic P fractions in rhizosphere and bulk soils of mono-specific Simon poplar （Populus simonii） and Mongolian pine （Pinus sylvestris var. mongolica） plantations in a semiarid sandy soil of Horqin Sandy Land in Northeast China. Total organic P （TPo） accounted for 76% of total P across the two stands. The concentration of organic P （Po） fractions decreased in the order of NaOH-Po〉Res-Po〉HCI-Po〉NaHCO3-Po in both plantations. The concentration of NaHCO3-Po was 38% and 43% lower in rhizosphere soil than in bulk soil in Simon poplar and Mongolian pine plantations, respectively. In contrast, total P, TPo and NaOH-Po significantly accumulated in rhizosphere soil in Simon poplar plantations, but no change in Mongolian pine plantations. Soil recalcitrant organic P fractions were positively correlated with soil organic carbon. The results suggest that rhizosphere labile organic P was an important source of plant-available P in this semiarid region, but the dynamic of rhizosphere recalcitrant organic P fractions varied with tree species and was correlated to organic carbon dynamics.
