Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effec...Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effects on recurrent spontaneous abortion and that can maintain pregnancy until delivery. It was hypothesized that this herbal medicine can also prolong allograft survival after organ transplantation. Here,in an in vivo study,we report the anti-rejection effect of the ethanol extract of Poria cocos Wolf (EEPCW) in rats after cardiac allograft implantation. Methods Ten normal rats were healthy controls. Eighty rats receiving homologous heart transplants were divided into 4 groups of 20 rats each based on type of treatment: olive oil 8 ml·kg -1 ·d -1 ,EEPCW 25 mg·kg -1 ·d -1 , EEPCW 50 mg·kg -1 ·d -1 or cyclosporin A 5mg·kg -1 ·d -1 . Allograft survival was observed in 10 rats from each group. On the seventh day post transplantation,pathological lesions and percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and the CD_4 +/CD_8 + ratio in peripheral blood were assessed in another 10 rats from each group and in 10 normal rats. Results The survival time of donor hearts in the two EEPCW groups was significantly prolonged,to (15.9±2.4) days and (30.0±0.0) days,respectively,compared with (6.7±0.8) days in the control group. Pathological lesions in the two EEPCW groups were also less severe,and the percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and CD_4 +/CD_8 + ratio were significantly lower in the EEPCW groups. Conclusions Acute rejection of heart transplants and cellular immune reaction can be effectively suppressed using the EEPCW. Taking advantage of novel immunosuppressants derived from Chinese medicinal herbs used to treat abnormal pregnancy provides a hopeful road for future research and treatment in organ transplantation.展开更多
为了探究茯苓配方(茯苓、山楂、黄大茶)浸膏对高脂饮食小鼠肥胖及脂质沉积的影响,将C57/BL6N小鼠随机分为对照组,模型组,奥利司他药物对照组,茯苓配方(Poria cocos Wolf formula,PWF)浸膏低、高剂量组。除对照组给予低脂饲料,其他组均...为了探究茯苓配方(茯苓、山楂、黄大茶)浸膏对高脂饮食小鼠肥胖及脂质沉积的影响,将C57/BL6N小鼠随机分为对照组,模型组,奥利司他药物对照组,茯苓配方(Poria cocos Wolf formula,PWF)浸膏低、高剂量组。除对照组给予低脂饲料,其他组均以高脂饲料喂养12周。每周记录小鼠的体重、能量摄入。给药结束后,计算各组小鼠Lee’s指数、脏器系数,采用苏木精-伊红(hematoxylin-eosin,HE)染色法观察各组小鼠肝细胞与脂肪细胞形态变化,全自动生化仪测定各组小鼠血清中TG、TC、HDL-C、LDL-C、AST及ALT水平变化。结果显示,与对照组相比,模型组小鼠体重、Lee’s指数、脏器系数、TG、TC、LDL-C、AST及ALT水平均显著升高(P<0.01或P<0.001),肝脏脂滴数量与脂肪细胞直径增加;与模型组相比,茯苓配方浸膏高剂量组小鼠体重、Lee’s指数和脏器系数均显著降低(P<0.05或P<0.01),低剂量组小鼠体重、Lee’s指数和脏器系数均没有显著变化,茯苓配方浸膏高剂量组小鼠肝脏脂滴数量与脂肪细胞直径减少,TG、TC、LDL-C水平、AST及ALT水平均显著降低(P<0.05或P<0.01或P<0.001)。综上所述,茯苓配方浸膏能够改善高脂饮食小鼠的体重及脂质沉积,为开发改善肥胖的天然物质提供理论基础。展开更多
了解近10年茯苓科学研究的现状,以中国期刊全文数据库和Web of Science为文献来源,检索了2011—2022年茯苓研究的相关文献,分别对其发表年份、被引来源、第一作者机构、区域分布及研究主题等进行统计学分析;并利用文献计量工具VOSviewe...了解近10年茯苓科学研究的现状,以中国期刊全文数据库和Web of Science为文献来源,检索了2011—2022年茯苓研究的相关文献,分别对其发表年份、被引来源、第一作者机构、区域分布及研究主题等进行统计学分析;并利用文献计量工具VOSviewer研究文献关键词共现网络特性。结果表明,近10年茯苓研究相关论文年发表量波动较大,研究机构集中在国内,与茯苓产地分布关系密切。对茯苓的研究主题广泛,其中药理是发文量最多的一个主题,且已形成较稳定的研究机构和团队。但茯苓的菌种资源退化,栽培技术和精深加工产品开发等仍限制我国茯苓产业的发展,应加强茯苓有性育种、构效关系和健康产品功效研究,促进茯苓产业的可持续发展。展开更多
Objective:Poria cocos and Polyporus umbellatus are similar medicinal fungi in traditional Chinese medicines.A method for fingerprint analysis of monosaccharide composition of polysaccharides by HPLC combined with chem...Objective:Poria cocos and Polyporus umbellatus are similar medicinal fungi in traditional Chinese medicines.A method for fingerprint analysis of monosaccharide composition of polysaccharides by HPLC combined with chemometrics methods has been developed for characterization and discrimination of them in this research.Methods:The polysaccharides were extracted by decocting in water,and then completely hydrolyzed with hydrochloride.Monosaccharides in the hydrolyzates were derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP)for HPLC analysis.More than 20 batches of P.cocos and P.umbellatus from different regions were analyzed.Results:The fingerprints of P.cocos showed five common characteristic peaks,which were identified by comparing with the reference substances.The five peaks corresponded to the derivatives of mannose,ribose,glucose,galactose,and fucose.At the same time,the fingerprints of P.umbellatus showed eight common characteristic peaks,of which seven were identified as the derivatives of mannose,ribose,rhamnose,glucose,galactose,xylose,and fucose.Moreover,the similarity of their fingerprints was respectively calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM published by China Pharmacopoeia Committee(Version 2004 A).And the data were further processed by hierarchical cluster analysis(HCA)and principal component analysis(PCA).The similarity evaluation and HCA indicated that there were no significant difference in P.cocos or P.umbellatus samples from different geographical regions,but PCA was performed to characterize the difference in monosaccharide constituents between P.cocos and P.umbellatus,and linear discriminant analysis(LDA)showed the overall correct classification rate was 100%.Conclusion:The fingerprint analysis method of monosaccharide composition of water-soluble polysaccharides can distinguish P.cocos and P.umbellatus,and can be applied for the authentication or quality control for P.cocos and P.umbellatus.展开更多
目的研究茯苓原生质体制备及其再生条件。方法采用不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生条件进行研究。结果不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生产生不同影响。结论Z10-2原生质体...目的研究茯苓原生质体制备及其再生条件。方法采用不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生条件进行研究。结果不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生产生不同影响。结论Z10-2原生质体制备时菌丝最适菌龄为72 h,酶液浓度为3%,稳渗剂以0.5 m o l/L的甘露醇为佳。Z10-3原生质体制备时菌丝最适菌龄为56 h,酶液浓度为3%,稳渗剂以0.6 m o l/L的甘露醇为佳。Z10-2和Z10-3原生质体均以甘露醇作稳渗剂再生率较高,其最佳浓度为0.6 m o l/L。在该条件下Z10-2原生质体再生率为7.5×1-0 3,Z10-3为1.3×10-2。展开更多
文摘Background A living fetus within the maternal uterus provides an example of allogene tolerance in mammals. Poria cocos Wolf is the main component of many Chinese medicinal combination drugs that have therapeutic effects on recurrent spontaneous abortion and that can maintain pregnancy until delivery. It was hypothesized that this herbal medicine can also prolong allograft survival after organ transplantation. Here,in an in vivo study,we report the anti-rejection effect of the ethanol extract of Poria cocos Wolf (EEPCW) in rats after cardiac allograft implantation. Methods Ten normal rats were healthy controls. Eighty rats receiving homologous heart transplants were divided into 4 groups of 20 rats each based on type of treatment: olive oil 8 ml·kg -1 ·d -1 ,EEPCW 25 mg·kg -1 ·d -1 , EEPCW 50 mg·kg -1 ·d -1 or cyclosporin A 5mg·kg -1 ·d -1 . Allograft survival was observed in 10 rats from each group. On the seventh day post transplantation,pathological lesions and percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and the CD_4 +/CD_8 + ratio in peripheral blood were assessed in another 10 rats from each group and in 10 normal rats. Results The survival time of donor hearts in the two EEPCW groups was significantly prolonged,to (15.9±2.4) days and (30.0±0.0) days,respectively,compared with (6.7±0.8) days in the control group. Pathological lesions in the two EEPCW groups were also less severe,and the percentages of CD_3 +,CD_4 +,and CD_8 + lymphocytes and CD_4 +/CD_8 + ratio were significantly lower in the EEPCW groups. Conclusions Acute rejection of heart transplants and cellular immune reaction can be effectively suppressed using the EEPCW. Taking advantage of novel immunosuppressants derived from Chinese medicinal herbs used to treat abnormal pregnancy provides a hopeful road for future research and treatment in organ transplantation.
文摘为了探究茯苓配方(茯苓、山楂、黄大茶)浸膏对高脂饮食小鼠肥胖及脂质沉积的影响,将C57/BL6N小鼠随机分为对照组,模型组,奥利司他药物对照组,茯苓配方(Poria cocos Wolf formula,PWF)浸膏低、高剂量组。除对照组给予低脂饲料,其他组均以高脂饲料喂养12周。每周记录小鼠的体重、能量摄入。给药结束后,计算各组小鼠Lee’s指数、脏器系数,采用苏木精-伊红(hematoxylin-eosin,HE)染色法观察各组小鼠肝细胞与脂肪细胞形态变化,全自动生化仪测定各组小鼠血清中TG、TC、HDL-C、LDL-C、AST及ALT水平变化。结果显示,与对照组相比,模型组小鼠体重、Lee’s指数、脏器系数、TG、TC、LDL-C、AST及ALT水平均显著升高(P<0.01或P<0.001),肝脏脂滴数量与脂肪细胞直径增加;与模型组相比,茯苓配方浸膏高剂量组小鼠体重、Lee’s指数和脏器系数均显著降低(P<0.05或P<0.01),低剂量组小鼠体重、Lee’s指数和脏器系数均没有显著变化,茯苓配方浸膏高剂量组小鼠肝脏脂滴数量与脂肪细胞直径减少,TG、TC、LDL-C水平、AST及ALT水平均显著降低(P<0.05或P<0.01或P<0.001)。综上所述,茯苓配方浸膏能够改善高脂饮食小鼠的体重及脂质沉积,为开发改善肥胖的天然物质提供理论基础。
文摘了解近10年茯苓科学研究的现状,以中国期刊全文数据库和Web of Science为文献来源,检索了2011—2022年茯苓研究的相关文献,分别对其发表年份、被引来源、第一作者机构、区域分布及研究主题等进行统计学分析;并利用文献计量工具VOSviewer研究文献关键词共现网络特性。结果表明,近10年茯苓研究相关论文年发表量波动较大,研究机构集中在国内,与茯苓产地分布关系密切。对茯苓的研究主题广泛,其中药理是发文量最多的一个主题,且已形成较稳定的研究机构和团队。但茯苓的菌种资源退化,栽培技术和精深加工产品开发等仍限制我国茯苓产业的发展,应加强茯苓有性育种、构效关系和健康产品功效研究,促进茯苓产业的可持续发展。
基金supported by Shanghai Biotechnology Support Project(Grant No.16401900800)National Science and Technology Project(Grant No.2018ZX09721003-009-011).
文摘Objective:Poria cocos and Polyporus umbellatus are similar medicinal fungi in traditional Chinese medicines.A method for fingerprint analysis of monosaccharide composition of polysaccharides by HPLC combined with chemometrics methods has been developed for characterization and discrimination of them in this research.Methods:The polysaccharides were extracted by decocting in water,and then completely hydrolyzed with hydrochloride.Monosaccharides in the hydrolyzates were derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP)for HPLC analysis.More than 20 batches of P.cocos and P.umbellatus from different regions were analyzed.Results:The fingerprints of P.cocos showed five common characteristic peaks,which were identified by comparing with the reference substances.The five peaks corresponded to the derivatives of mannose,ribose,glucose,galactose,and fucose.At the same time,the fingerprints of P.umbellatus showed eight common characteristic peaks,of which seven were identified as the derivatives of mannose,ribose,rhamnose,glucose,galactose,xylose,and fucose.Moreover,the similarity of their fingerprints was respectively calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM published by China Pharmacopoeia Committee(Version 2004 A).And the data were further processed by hierarchical cluster analysis(HCA)and principal component analysis(PCA).The similarity evaluation and HCA indicated that there were no significant difference in P.cocos or P.umbellatus samples from different geographical regions,but PCA was performed to characterize the difference in monosaccharide constituents between P.cocos and P.umbellatus,and linear discriminant analysis(LDA)showed the overall correct classification rate was 100%.Conclusion:The fingerprint analysis method of monosaccharide composition of water-soluble polysaccharides can distinguish P.cocos and P.umbellatus,and can be applied for the authentication or quality control for P.cocos and P.umbellatus.
文摘目的研究茯苓原生质体制备及其再生条件。方法采用不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生条件进行研究。结果不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生产生不同影响。结论Z10-2原生质体制备时菌丝最适菌龄为72 h,酶液浓度为3%,稳渗剂以0.5 m o l/L的甘露醇为佳。Z10-3原生质体制备时菌丝最适菌龄为56 h,酶液浓度为3%,稳渗剂以0.6 m o l/L的甘露醇为佳。Z10-2和Z10-3原生质体均以甘露醇作稳渗剂再生率较高,其最佳浓度为0.6 m o l/L。在该条件下Z10-2原生质体再生率为7.5×1-0 3,Z10-3为1.3×10-2。
