In recent years,the development of functional packaging from natural active compounds has been a promising strategy for microbial control in foods.In this study,the basil essential oil(BEO)was applied as the principal...In recent years,the development of functional packaging from natural active compounds has been a promising strategy for microbial control in foods.In this study,the basil essential oil(BEO)was applied as the principal antibacterial agent,and a controlled-release nanofibrous system was engineered with responsiveness to bacterial phospholipase.It indicated that encapsulation by cationic liposome significantly improved the dispersity and stability of BEO during the electrospinning process,and the prepared BEO-loaded cationic liposomes(BCLs)could maintain structural integrity in polymer nanofibers.The resulting BCLs-loaded nanofibers showed an effective antibacterial activity against L.monocytogenes.Additionally,it is worth noting that incorporation of soybean lecithin in nanofiber substrate could accelerate the release of loaded antibacterial agents when exposed to L.monocytogenes,thus obtaining an enhanced antibacterial activity.This could be attributed to the increased sensitivity of nanofibers to the degradation by bacterial phospholipases after incorporation with soybean lecithin.Finally,the application test showed that the prepared antibacterial nanofibrous mats could help maintain the quality of chilled pork during 4-days storage,which indicated their promising potential as active packaging.Given the serious threat of L.monocytogenes to food safety,the present antibacterial nanofibers have wide application prospect for improving microbial safety of chilled meat.展开更多
基金This research project was financially supported by State Key Laboratory of Utilization of Woody Oil Resource(Grant no.2019XK 2002)National Natural Science Foundation of China(Grant no.31972172)+2 种基金Natural Science Foundation of Jiangsu Province(Grant no.BK20201417)Jiangsu Province Research Fund(Grant no.JNHB-131)Jiangsu University Research Fund(Grant no.11JDG050).
文摘In recent years,the development of functional packaging from natural active compounds has been a promising strategy for microbial control in foods.In this study,the basil essential oil(BEO)was applied as the principal antibacterial agent,and a controlled-release nanofibrous system was engineered with responsiveness to bacterial phospholipase.It indicated that encapsulation by cationic liposome significantly improved the dispersity and stability of BEO during the electrospinning process,and the prepared BEO-loaded cationic liposomes(BCLs)could maintain structural integrity in polymer nanofibers.The resulting BCLs-loaded nanofibers showed an effective antibacterial activity against L.monocytogenes.Additionally,it is worth noting that incorporation of soybean lecithin in nanofiber substrate could accelerate the release of loaded antibacterial agents when exposed to L.monocytogenes,thus obtaining an enhanced antibacterial activity.This could be attributed to the increased sensitivity of nanofibers to the degradation by bacterial phospholipases after incorporation with soybean lecithin.Finally,the application test showed that the prepared antibacterial nanofibrous mats could help maintain the quality of chilled pork during 4-days storage,which indicated their promising potential as active packaging.Given the serious threat of L.monocytogenes to food safety,the present antibacterial nanofibers have wide application prospect for improving microbial safety of chilled meat.
